Article
Biology
Kavitha Chinnaiya, Sarah Burbridge, Aragorn Jones, Dong Won Kim, Elsie Place, Elizabeth Manning, Ian Groves, Changyu Sun, Matthew Towers, Seth Blackshaw, Marysia Placzek, Elisabeth Knust
Summary: This study reveals that the tuberal hypothalamus is generated from hypothalamic floor plate-like cells. A stream of tuberal progenitors develops in the neural tube as a wave of BMP signalling sweeps through the hypothalamic floor plate. The BMP signalling initiates tuberal progenitor specification, but must be eliminated for these to progress to anterior neurogenic progenitors. This study provides an integrated account of the development of the tuberal hypothalamus.
Article
Multidisciplinary Sciences
Rachel K. Lex, Weiqiang Zhou, Zhicheng Ji, Kristin N. Falkenstein, Kaleigh E. Schuler, Kathryn E. Windsor, Joseph D. Kim, Hongkai Ji, Steven A. Vokes
Summary: The study challenges the current understanding that GLI3 repression is established before Hedgehog (HH) signaling and demonstrates that GLI3 is inert in the limb bud before HH onset. The loss of Gli3 does not increase target gene expression or accessibility, contrasting with post-HH signaling. GLI repression is established independently of HH signaling, but after its onset.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Max D. Knickmeyer, Juan L. Mateo, Stephan Heermann
Summary: The study demonstrates that BMP plays a significant role in optic chiasm formation and RGC axon pathfinding in zebrafish, potentially through an interaction with midline guidance cues and optic stalk morphogenesis. Induction of BMP4 at different time points results in distinct neurodevelopmental abnormalities, severely affecting RGC axon projections in different ways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Huishan Wang, Chencheng Yang, Yuwei Li, Shuhua Zhao, Pengcheng Ma, Bingyu Mao
Summary: The study revealed that RNF220 fine tunes Shh signaling by regulating Glis polyubiquitination and nuclear translocation, while also affecting Shh signaling through EED in cerebellar development. In the cytoplasm, RNF220-induced Gli accumulation in the cytoskeletal fraction and degradation via autophagy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Neurosciences
Elsie Place, Elizabeth Manning, Dong Won Kim, Arisa Kinjo, Go Nakamura, Kyoji Ohyama
Summary: Pro-opiomelanocortin (POMC)-expressing neurons in the hypothalamic arcuate nucleus (ARC) play important roles in feeding and energy homeostasis. The expression and regulation of transcription factors implicated in epithelial-to-mesenchymal transitions (EMTs) were characterized during POMC neurogenesis in the embryonic chick. The study showed that SOX2, SOX9, PROX1, ISL1, and SOX11 were sequentially expressed in the developmental process, and that Sonic Hedgehog (SHH) and Notch signaling pathways converged to regulate hypothalamic cellular homeostasis.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Oncology
Somanath Kundu, Mohan S. Nandhu, Sharon L. Longo, John A. Longo, Shawn Rai, Lawrence S. Chin, Timothy E. Richardson, Mariano S. Viapiano
Summary: DLG5 is upregulated in malignant gliomas and promotes invasion, viability, and self-renewal of glioblastoma stem cells. It regulates Sonic Hedgehog (Shh) signaling, making DLG5-deficient cells insensitive to Shh ligand, and its loss increases the proteasomal degradation of Gli1, leading to tumor stem cell sensitization. These findings reveal a novel and pro-tumoral role for DLG5 in glioblastoma, distinct from its proposed tumor-suppressor role in other solid tumors.
Article
Agriculture, Dairy & Animal Science
Xiaojing Qin, Xinfeng Liu, Xingyue Yan, Meizhen Long, Zixu Wang, Yulan Dong, Yaoxing Chen, Jing Cao
Summary: The study found that melatonin plays a role in regulating the secretion of somatostatin in chicks under different monochromatic lights, with exposure to red light leading to higher levels of somatostatin in the hypothalamus and pituitary.
Article
Neurosciences
Osher Ben-Nun, Tatiana Kisliouk, Asaf Marco, Tali Rosenberg, Noam Meiri
Summary: Stressful environmental events in early life have lasting consequences on later stress responses. Previous heat conditioning during the critical period of heat-response development in 3-day-old chicks leads to heat vulnerability later in life. Early-life heat stress affects the inflammatory response in the chick anterior hypothalamus, specifically impacting hypothalamic microglia.
Article
Pediatrics
Pu Siyu, Wang Junxiang, Wang Qi, Zhang Yimao, Jin Shuguang
Summary: The nuclear transcription factor GLI1/GLI2 of the Shh signaling pathway plays a regulatory role in epithelial-mesenchymal transition (EMT) related to hepatic fibrosis in patients with biliary atresia (BA).
FRONTIERS IN PEDIATRICS
(2022)
Article
Pathology
Julian Henao-Restrepo, Yudys Anggelly Caro-Urrego, Lina Marcela Barrera-Arenas, Juan Carlos Arango-Viana, Maria Bermudez-Munoz
Summary: The differential expression of proteins from the PI3K/Akt/mTORC1 and SHH/GLI pathways in human gliomas suggests that activation of these signaling networks is associated with glioma progression, varying according to tumor type and grade.
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2021)
Review
Cell & Tissue Engineering
Xi-Min Hu, Zhi-Xin Li, Dan-Yi Zhang, Yi-Chao Yang, Shen-ao Fu, Zai-Qiu Zhang, Rong-Hua Yang, Kun Xiong
Summary: Hair follicle stem cells (HFSCs) are valuable resources for studying adult stem cells and hair loss therapy, with potential therapeutic targets for skin and hair disorders. Multiple signaling pathways are involved in regulating the fate of HFSCs, providing a better understanding of pathophysiological changes in the HFSC niche.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Hung-Yu Shih, Chia-Wei Chang, Yi-Chieh Chen, Yi-Chuan Cheng
Summary: Bone morphogenetic protein (BMP) signaling plays a crucial role in the regulation of neural induction, neuronal specification, and neuronal differentiation. However, its role in neural progenitors is still not well understood. By manipulating BMP signaling in zebrafish embryos, researchers discovered that inhibiting BMP signaling during gastrulation can increase the proliferation of neural progenitors and decrease the number of neuronal precursors. This study also revealed that there is a fine balance between BMP and Notch signaling in regulating the proliferation of neural progenitor cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Julie Gamart, Iros Barozzi, Frederic Laurent, Robert Reinhardt, Laurene Ramos Martins, Thomas Oberholzer, Axel Visel, Rolf Zeller, Aimee Zuniga
Summary: SMAD4 regulates gene expression in early limb buds, primarily mediating BMP signal transduction and playing a crucial role in upregulating target genes in anterior limb bud mesenchyme, while also repressing posterior gene expression. This study reveals opposing trans-regulatory inputs from SHH- and SMAD4-mediated BMP signal transduction during digit patterning and outgrowth in early limb buds.
Article
Biochemistry & Molecular Biology
Bailey Halter, Nicholas Ildari, Mark A. Cline, Elizabeth R. Gilbert
Summary: Ferulic acid (FA) was found to have transient appetite suppressing effects on food intake in chicks, possibly mediated through the hypothalamic arcuate nucleus (ARC) involving POMC. This effect did not impact defecation frequency or other behaviors in the chicks.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Anatomy & Morphology
Taiki Katsuyama, Minori Kadoya, Manabu Shirai, Noriaki Sasai
Summary: Sox14 transcription factor plays a crucial role in promoting neuronal differentiation in the chick neural tube, acting as a modulator of cell proliferation. Its expression is initially in the progenitor regions before becoming confined to the V2a region, where it is involved in regulating the differentiation of neurons. Overexpression of Sox14 restricts progenitor cell proliferation, while blocking its expression inhibits neuronal differentiation in the V2a region.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Developmental Biology
Pamela S. Ellis, Sarah Burbridge, Sandrine Soubes, Kyoji Ohyama, Nadav Ben-Haim, Canhe Chen, Kim Dale, Michael M. Shen, Daniel Constam, Marysia Placzek
Article
Developmental Biology
Victor Muthu, Helen Eachus, Pam Ellis, Sarah Brown, Marysia Placzek
Article
Biochemistry & Molecular Biology
Helen Eachus, Charlotte Bright, Vincent T. Cunliffe, Marysia Placzek, Jonathan D. Wood, Penelope J. Watt
HUMAN MOLECULAR GENETICS
(2017)
Article
Biology
Ioannis Kasioulis, Raman M. Das, Kate G. Storey
Correction
Neurosciences
Fang Liu, Marysia Placzek, Hong Xu
NEUROSCIENCE LETTERS
(2014)
Article
Multidisciplinary Sciences
Raman M. Das, Kate G. Storey
Article
Endocrinology & Metabolism
Travis Fu, Caroline Pearson, Matthew Towers, Marysia Placzek
JOURNAL OF NEUROENDOCRINOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Christine M. Tait, Kavitha Chinnaiya, Elizabeth Manning, Mariyam Murtaza, John-Paul Ashton, Nicholas Furley, Chris J. Hill, C. Henrique Alves, Jan Wijnholds, Kai S. Erdmann, Andrew Furley, Penny Rashbass, Raman M. Das, Kate G. Storey, Marysia Placzek
Article
Multidisciplinary Sciences
Gabriela Toro-Tapia, Raman M. Das
Review
Biology
Ian Groves, Marysia Placzek, Alexander G. Fletcher
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Article
Developmental Biology
Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Summary: This study identified the signals guiding the transition of human NMP-like cells towards thoracic ventral spinal cord neurectoderm and defined an optimized protocol for generating thoracic MNs, facilitating the comparison of hPSC-derived spinal cord cells of distinct axial identities.
Article
Cell Biology
Helen Eachus, Lara Oberski, Jack Paveley, Irina Bacila, John-Paul Ashton, Umberto Esposito, Fayaz Seifuddin, Mehdi Pirooznia, Eran Elhaik, Marysia Placzek, Nils P. Krone, Vincent T. Cunliffe
Summary: This article investigates glucocorticoid resistance commonly observed in depression, which has been linked to reduced expression and/or function of the glucocorticoid receptor (GR). By comparing the brain methylomes and transcriptomes of wild-type and GR-mutant zebrafish, the authors identify 249 differentially methylated regions (DMRs) regulated by GR. They also find GR-regulated genes associated with chaperone-mediated protein folding, circadian rhythm regulation, metabolism regulation, and behavior, which are known to be related to unipolar depression and anxiety. The findings provide insights into the molecular mechanisms through which GR mediates stress responses in the adult zebrafish brain and support the use of the zebrafish GR mutant as a model for studying affective disorders.
DISEASE MODELS & MECHANISMS
(2023)
Article
Multidisciplinary Sciences
Sofia Sedas Perez, Caitlin Mcqueen, Holly Stainton, Joseph Pickering, Kavitha Chinnaiya, Patricia Saiz-Lopez, Marysia Placzek, Maria A. Ros, Matthew Towers
Summary: This study demonstrates the important role of fibroblast growth factors in triggering a mesodermal program that is essential for the timing of limb patterning. Additionally, it uncovers additional functions of Fgf signaling in maintaining gene expression and suppressing muscle development.
NATURE COMMUNICATIONS
(2023)
Article
Developmental Biology
Travis Fu, Matthew Towers, Marysia A. Placzek
Article
Physiology
Sarah Burbridge, Iain Stewart, Marysia Placzek
COMPREHENSIVE PHYSIOLOGY
(2016)