Article
Immunology
Robert Ossendorff, Sibylle Grad, Tobias Tertel, Dieter C. Wirtz, Bernd Giebel, Verena Boerger, Frank A. Schildberg
Summary: Characterized mesenchymal stem/stromal cell-derived extracellular vesicles (MSC-EVs) have a positive effect on chondrocyte matrix production and show potential for treating osteoarthritis (OA). However, the functional heterogeneity of MSC-EV preparations needs to be addressed.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Simon Rosowski, Caroline Remmert, Maren Marder, Misao Akishiba, Judith Bushe, Annette Feuchtinger, Alina Platen, Siegfried Ussar, Fabian Theis, Sandra Wiedenmann, Matthias Meier
Summary: This article presents a comprehensive single-cell transcriptomic study on in vitro neovascularization. Human induced pluripotent stem cells were differentiated into endothelial cells in 3D suspension culture, and various 3D microenvironmental approaches were investigated. The study provides insights into cell type development, stability, and plasticity, as well as cell-cell interactions and angiogenic signals during tubulogenesis.
Article
Biochemistry & Molecular Biology
Rimma Sergeevna Kamentseva, Marianna Viktorovna Kharchenko, Gulnara Vladikovna Gabdrahmanova, Michael Alexandrovich Kotov, Vera Vladislavovna Kosheverova, Elena Sergeevna Kornilova
Summary: This study compared the effects of EGF, TGF-α, and AREG on physiological responses in endometrial MSCs and HeLa cells, and investigated the internalization and degradation of EGFR stimulated by these ligands. The study found that while EGF and TGF-α stimulated cell proliferation and prevented differentiation in enMSCs in an EGFR-dependent manner, AREG had no effect. Additionally, differences were observed in the degradation and recovery of EGFR in different cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Maryana Teufelsbauer, Clemens Lang, Adelina Plangger, Barbara Rath, Doris Moser, Clement Staud, Christine Radtke, Christoph Neumayer, Gerhard Hamilton
Summary: Metformin is found to inhibit the proliferation of BM-MSCs and reduce the migration of breast cancer cell lines. The effects of conditioned media from drug-pretreated BM-MSCs on breast cancer cell lines vary depending on the specific combinations. Metformin may affect metastasis by altering the expression of adipokines and increasing osteogenic signaling in BM-MSCs' conditioned media.
Review
Cell & Tissue Engineering
Ahmed Lotfy, Noha M. AboQuella, Hongjun Wang
Summary: Mesenchymal stromal/stem cells (MSCs) are widely used in cell therapy due to their immunomodulatory and regenerative properties. The production of various factors via extracellular vesicles, especially exosomes, governs the efficacy of MSCs. MSC-derived exosomes (MSC-Exos) show advantages over MSCs themselves by reducing adverse effects. Clinical studies are assessing the therapeutic effect of MSC-Exos in different diseases, and production methods, dosage, and administration routes are being explored.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Chemistry, Multidisciplinary
J. Vos, H. Tejeda-Mora, A. Merino, L. Wu, W. W. Woud, J. A. A. Demmers, W. F. J. van IJcken, M. E. J. Reinders, M. J. Hoogduijn
Summary: This study examines the bio-distribution and longevity of membrane particles (MP) derived from human mesenchymal stromal cells (MSC) after intravenous administration in mice. It is found that MP mainly distribute in plasma and leukocytes, remaining in circulation for up to 24 hours, and impact liver function inducing metabolic pathways.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Cell Biology
Cansu Gorgun, Chiara Africano, Maria Chiara Ciferri, Nadia Bertola, Daniele Reverberi, Rodolfo Quarto, Silvia Ravera, Roberta Tasso
Summary: This study demonstrates that EVs derived from MSCs preconditioned with hypoxia inflammatory cytokines can attenuate inflammaging and alleviate age-related inflammation and mitochondrial dysfunction.
Review
Biology
Arulkumar Nallakumarasamy, Madhan Jeyaraman, Nicola Maffulli, Naveen Jeyaraman, Veerasivabalan Suresh, Srinath Ravichandran, Manu Gupta, Anish G. Potty, Saadiq F. El-Amin, Manish Khanna, Ashim Gupta
Summary: This article discusses the role of stem cell therapy and exosomes in promoting tissue regeneration in the wound healing process, as well as the application of different types of exosomes on three-dimensional bioscaffolds, providing new approaches for the management of chronic wounds.
Article
Pharmacology & Pharmacy
Isabel Hamshaw, Marco M. D. Cominetti, Wing-Yee Lai, Mark Searcey, Anja Mueller
Summary: Cancer metastasis is a major cause of cancer-related death. The CXCR4 receptor and its ligand CXCL12 play important roles in promoting metastasis, making them attractive therapeutic targets. In this study, a novel CXCR4 antagonist was developed and used for CXCR4-based imaging. The results showed that these antagonists were non-toxic and effectively inhibited cancer cell migration and calcium release.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wu-Xun Chen, Nai-Chen Cheng, Yan-Jun Chen, I-Chi Lee
Summary: In this study, silk fibroin-based microneedle patches were fabricated as carriers for adipose stem cell recruitment. The crystallinity of silk fibroin-based microneedles was determined after water annealing treatment. The results showed that the microneedle patches had sufficient strength to penetrate the skin and demonstrated sustained release of the model drug. Dual-layer microneedles loaded with SDF-1 were prepared to enhance drug release for stem cell recruitment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Immunology
Michele Aramburu Serafini, Dienifer Hermann Sirena, Ana Beatriz Tittoni da Silveira, Monique Franco-da-Silva, Mariana Rauback Aubin, Tuane Nerissa Alves Garcez, Anelise Araujo, Fernanda dos Santos Pereira, Martin J. Hoogduijn, Fabiany da Costa Goncalves, Ana Helena Paz
Summary: Inflammatory bowel diseases (IBD), including ulcerative colitis, are chronic inflammations of the gastrointestinal tract. Mesenchymal stromal cells (MSC) have shown promise as a therapy for IBD, but practical complexities arise from using living cells. This study investigated the potential of MSC-derived membrane particles (MP) and conditioned media (CM) as cell-free therapies for IBD, and found that they ameliorated colitis symptoms in a mouse model.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Cell & Tissue Engineering
Davod Pashoutan Sarvar, Hosein Effatpanah, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: The study highlights the important role of MSC-derived extracellular vesicles (MSC-EVs) in hematopoietic regulation and immune response after transplantation. MSC-EVs directly fuse with hematopoietic stem cell membranes, affecting their fate, and regulate the proliferation and differentiation of hematopoietic stem cells through the release of various bioactive factors.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Pharmacology & Pharmacy
Chiara Valsecchi, Stefania Croce, Elisa Lenta, Gloria Acquafredda, Patrizia Comoli, Maria Antonietta Avanzini
Summary: Clinical applications of Mesenchymal Stromal Cells (MSCs) have been widely reported, showing therapeutic potential in various diseases. MSCs, isolated from human tissues and easily expanded in vitro, have the ability to differentiate into various cell types and interact with immunological cells, exhibiting immunosuppressive and tissue repair properties. Extracellular Vesicles (EVs) released by MSCs have shown great potential in the treatment of damaged tissues and organs, as well as modulation of the immune system. EV-based therapies have advantages in crossing barriers and are not influenced by the surrounding environment.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Materials Science, Biomaterials
Jong Keun Seon, Sree Samanvitha Kuppa, Ju Yeon Kang, Jun Sik Lee, Su A. Park, Taek Rim Yoon, Kyung Soon Park, Hyung Keun Kim
Summary: Introduces a novel peptide called bone-forming peptide-5 (BFP-5), derived from SDF-1, that promotes osteogenesis of MSCs and bone formation and healing. MSCs treated with BFP-5 showed enhanced alizarin red S staining and higher ALP activity. Animal models transplanted with PCL scaffolds coated with BFP-5 demonstrated significantly greater bone formation compared to animals transplanted with PCL scaffolds alone. These findings suggest the potential use of BFP-5 in the development of therapies for bone-related conditions.
BIOMATERIALS SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Kayla Templeton, Meiby Ramos, Jacqueline Rose, Bryan Le, Qingwen Zhou, Amin Cressman, Stephanie Ferreyra, Carlito B. Lebrilla, Fernando Antonio Fierro
Summary: The study demonstrates that sialylation of N-glycans in MSCs is tightly regulated in response to environmental cues, with the inhibition of sialylation by 3F-Neu5Ac resulting in increased adhesion, migration, and survival of MSCs, while also inhibiting osteogenic and adipogenic differentiation. Additionally, the secretion of key trophic factors by MSCs is variably affected upon exposure to 3F-Neu5Ac, suggesting glycoengineering MSCs to reduce sialylated N-glycans could enhance their therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Natasha M. J. Perepelkin, Kirsten Hayward, Tumelo Mokoena, Michael J. Bentley, Lisa U. Ross-Rodriguez, Leah Marquez-Curtis, Locksley E. McGann, Jelena L. Holovati, Janet A. W. Elliott
CELL AND TISSUE BANKING
(2016)
Article
Biology
Richelle C. Prickett, Leah A. Marquez-Curtis, Janet A. W. Elliott, Locksley E. McGann
Review
Biology
Leah A. Marquez-Curtis, Anna Janowska-Wieczorek, Locksley E. McGann, Janet A. W. Elliott
Article
Biology
Leah A. Marquez-Curtis, A. Billal Sultani, Locksley E. McGann, Janet A. W. Elliott
Article
Multidisciplinary Sciences
A. Billal Sultani, Leah A. Marquez-Curtis, Janet A. W. Elliott, Locksley E. McGann
SCIENTIFIC REPORTS
(2016)
Article
Biotechnology & Applied Microbiology
Megan Dodd, Leah Marquez-Curtis, Anna Janowska-Wieczorek, Gonzalo Hortelano
JOURNAL OF GENE MEDICINE
(2014)
Article
Oncology
Hilal Gul-Uludag, Juliana Valencia-Serna, Cezary Kucharski, Leah A. Marquez-Curtis, Xiaoyan Jiang, Loree Larratt, Anna Janowska-Wieczorek, Hasan Uludag
Article
Biotechnology & Applied Microbiology
Bahareh Sayyar, Megan Dodd, Leah Marquez-Curtis, Anna Janowska-Wieczorek, Gonzalo Hortelano
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Bahareh Sayyar, Megan Dodd, Leah Marquez-Curtis, Anna Janowska-Wieczorek, Gonzalo Hortelano
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2015)
Article
Biology
Nasim Eskandari, Leah A. Marquez-Curtis, Locksley E. McGann, Janet A. W. Elliott
Article
Multidisciplinary Sciences
Leah A. Marquez-Curtis, Xiao-Qing Dai, Yan Hang, Jonathan Y. Lam, James Lyon, Jocelyn E. Manning Fox, Locksley E. McGann, Patrick E. MacDonald, Seung K. Kim, Janet A. W. Elliott
Summary: The objective of this study was to optimize the cryopreservation of dissociated islet cells and obtain functional cells for single-cell transcriptome studies on diabetes. The optimized procedure maintained the integrity, molecular, and functional phenotypes of the cells. These findings are important for investigating cellular mechanisms in diabetes and for bio-engineering applications.
Article
Biology
Lanah Mohammed, Leah A. Marquez-Curtis, Janet A. W. Elliott
Summary: The cryopreservation of hCMEC using 5% Me2SO and 6% HES resulted in over 90% cell viability. Non-toxic glycerol was tested as a cryoprotective agent and a protocol was developed to optimize its effectiveness. The viability and functionality of cryopreserved cells were confirmed through various experiments.
Article
Biology
Sergio Enrique Tapia Lishner, Leah A. Marquez-Curtis, Janet A. W. Elliott
Summary: The cornea is a transparent tissue that helps the eye focus by bending light. Cryopreservation can increase availability of corneas for transplants, but suitable donor tissue is scarce. Understanding the transport of water and cryoprotectants in the tissue is crucial for developing effective preservation protocols.
Article
Biology
Leah A. Marquez-Curtis, Locksley E. McGann, Janet A. W. Elliott
Meeting Abstract
Biotechnology & Applied Microbiology
Laura Rose, Cezary Kucharski, Dulan Jayasooriya, Leah A. Marquez-Curtis, Hilal Gul-Uludag, Anna Janowska-Wieczorek, Hasan Uludag