Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Ivana Roberta Romano, Floriana D'Angeli, Nunzio Vicario, Cristina Russo, Carlo Genovese, Debora Lo Furno, Giuliana Mannino, Serena Tamburino, Rosalba Parenti, Rosario Giuffrida
Summary: This review discusses the osteogenic and chondrogenic differentiation ability of adipose-derived mesenchymal stromal cells (ASCs) and their potential therapeutic applications in bone and cartilage defects. ASCs can easily differentiate into chondrocyte-like and osteocyte-like elements and they can be used for tissue repair. Various strategies involving ASCs, such as transplantation of freshly harvested cells, in vitro expanded cells, or predifferentiated cells, are being developed globally. Combining ASCs with scaffolds can further enhance their positive effects and contribute to their osteogenic or chondrogenic differentiation.
Review
Cell Biology
Zi-Yi Feng, Shu-De Yang, Ting Wang, Shu Guo
Summary: Melatonin, synthesized in the pineal gland, controls the body's circadian rhythm and has various effects including regulating metabolism, providing neuroprotection, and acting as an anti-inflammatory and free radical scavenger. Research has shown that melatonin can play a role in regulating MSCs, which have the potential for tissue regeneration through paracrine signaling. MSC-derived extracellular vesicles (EVs) could potentially offer advantages over MSCs in regenerative medicine due to their ability to respond to injury and promote tissue regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Raphael Gorodetsky, Wilhelm K. Aicher
Summary: MSCs from different sources can release pro-regenerative and anti-inflammatory factors, with hPSCs from the fresh human placenta being a preferred source for abundant cell harvesting. The f-hPSCs from neonate tissues of the placenta can undergo extended expansion with low risk of senescence, making them competent cells for therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Thibault Canceill, Geraldine Jourdan, Philippe Kemoun, Christophe Guissard, Yanad Abou Monsef, Marion Bourdens, Benoit Chaput, Sandrine Cavalie, Louis Casteilla, Valerie Planat-Benard, Paul Monsarrat, Isabelle Raymond-Letron
Summary: Adipose-derived mesenchymal stromal cells (ASC) transplantation is a promising strategy for tissue regeneration. This study aimed to optimize the generation phase of a platelet-lysate-based fibrin hydrogel (PLFH) as a proper carrier for in situ ASC implantation, and comprehensively assess its in vitro properties and in vivo safety profile.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Niyaz Al-Sharabi, Reinhard Gruber, Mariano Sanz, Samih Mohamed-Ahmed, Einar K. Kristoffersen, Kamal Mustafa, Siddharth Shanbhag
Summary: The secretome of human bone marrow MSC (BMSC) was compared to that of leukocyte- and platelet-rich fibrin (L-PRF), in terms of wound healing and regeneration. The results showed that BMSC secretome had more differentially expressed proteins involved in wound healing, suggesting its potential for periodontal and bone regeneration. Further evaluation of the in vivo efficacy of this therapy is needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Giuliana Minani Bertolino, Marie Maumus, Christian Jorgensen, Daniele Noel
Summary: Extracellular vesicles (EVs) isolated from induced pluripotent stem cells (iMSCs) show promise in regenerative medicine for the treatment of various diseases. However, the heterogeneity between MSC donors may lead to batch-to-batch variability in the use of EVs as cell-free therapeutic agents.
Review
Cell Biology
Maria I. Quinones-Vico, Raquel Sanabria de la Torre, Manuel Sanchez-Diaz, Alvaro Sierra-Sanchez, Trinidad Montero-Vilchez, Ana Fernandez-Gonzalez, Salvador Arias-Santiago
Summary: The skin, the largest organ of the human body, has important functions including protection and maintenance. MSCs and their secretome, particularly exosomes, are believed to play a significant role in skin repair and regeneration, indicating the need for further research for clinical translation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Maximilian Handke, Anastasia Rakow, Debora Singer, Lea Miebach, Frank Schulze, Sander Bekeschus, Janosch Schoon, Georgi I. Wassilew
Summary: Bone marrow obtained during periacetabular osteotomy (PAO) may serve as a promising novel source for BM-MSCs from young donors. Compared to THA patients, PAO patients are younger and BM-MSCs have higher proliferation capacity and secrete more growth factors.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biology
Jerome Laloze, Alexis Desmouliere
Summary: Advances in regenerative medicine are closely connected to the use of adipose tissue-derived mesenchymal stem/stromal cells, with the primary importance of paracrine secretion. Exosomes, possessing similar properties as their source cells, are likely to become the main focus in tomorrow's cell therapy, offering advantages over direct cell use.
Article
Cell Biology
Tomoko Ishikura, Hideaki Shiga, Yuka Nakamura, Takako Kanitani, Yasuhito Ishigaki, Takaki Miwa
Summary: This study aimed to determine whether nasally administered murine adipose-derived stem cells (ADSCs) could support olfactory regeneration in vivo. The results showed that ADSCs can promote the repair of olfactory epithelium damage and enhance olfactory perception. This finding indicates that nasally administered ADSCs secreting neurotrophic factors can stimulate the regeneration of olfactory epithelium, thereby improving odor aversion behavior in vivo.
Review
Biochemistry & Molecular Biology
Katarzyna Czerwiec, Malgorzata Zawrzykraj, Milena Deptula, Aneta Skoniecka, Agata Tyminska, Jacek Zielinski, Adam Kosinski, Michal Pikula
Summary: Adipose-derived mesenchymal stromal cells (AD-MSCs) have gained significant attention due to their easy availability from fat tissue and their regenerative and immunomodulatory properties. These cells show great potential in stem cell-based therapies for wound healing, orthopedic, cardiovascular, and autoimmune diseases. Numerous clinical trials have provided evidence for the effectiveness of AD-MSCs. This article presents current knowledge on AD-MSCs, their applications in pre-clinical models and clinical studies, and highlights the potential for chemically or genetically modified AD-MSCs as the next generation of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Gabriele Storti, Evaldo Favi, Francesca Albanesi, Bong-Sung Kim, Valerio Cervelli
Summary: Kidney transplantation is the gold standard treatment for end-stage renal disease, but long-term allograft survival has not significantly improved. Ischemia-reperfusion injury is a major complication that affects transplant outcomes. Adipose stem/stromal cells are being studied for their potential in mitigating IRI impact and promoting tolerance in pre-clinical and clinical models of renal IRI and allograft rejection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Parisa Kangari, Leila Roshangar, Aida Iraji, Tahereh Talaei-Khozani, Mahboobeh Razmkhah
Summary: Shilajit promotes osteogenic differentiation of human adipose-derived mesenchymal stem cells in both 3D and 2D cultures. The combination of Shilajit and Alg hydrogel initiates osteogenic differentiation in a short period of time.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Letter
Critical Care Medicine
Sicheng Li, Xiuwen Wu, Jianan Ren
CRITICAL CARE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ye Liu, Jinjian Huang, Ziyan Xu, Sicheng Li, Yungang Jiang, Gui Wen Qu, Zongan Li, Yun Zhao, Xiuwen Wu, Jianan Ren
Summary: Polyacrylamide/gelatin/silver nanoparticle (PAAm-GelatinAgNPs) ink was designed to improve gelatin-based hydrogels by enhancing their mechanical properties and providing antibacterial capabilities. The ink's shear thinning property enabled the printing of complex 3D scaffolds for potential applications in customized therapy for antimicrobial and anti-UV damage to tissues.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Immunology
Wenbin Gong, Peizhao Liu, Tao Zheng, Xiuwen Wu, Yun Zhao, Jianan Ren
Summary: Syk, a cytoplasmic non-receptor protein tyrosine kinase, plays crucial roles in signal transduction in various cells, especially in gut diseases. Inhibitors targeting Syk show great potential for the treatment of gut diseases.
INTERNATIONAL REVIEWS OF IMMUNOLOGY
(2022)
Article
Chemistry, Physical
Guopu Chen, Yunru Yu, Xiao Fu, Gefei Wang, Zhiming Wang, Xiuwen Wu, Jianan Ren, Yuanjin Zhao
Summary: Metal organic frameworks (MOFs) are proposed as attractive materials for their adjustable structures and physicochemical properties, with a focus on developing functional materials with catalytic capabilities for biomedical values. Biocompatible manganese metal organic framework (Mn-MOF)-based catalase mimetics with microfluidic microcapsule encapsulation were presented for intravital inflammatory bowel disease (IBD) treatment. These Mn-MOF-encapsulated microcapsules showed high performance in treating spontaneous IBD in interleukin-10-deficient mice by relieving oxidative stress and reducing inflammation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Cell Biology
Qinjie Liu, Jie Wu, Xufei Zhang, Xuanheng Li, Xiuwen Wu, Yun Zhao, Jianan Ren
Summary: The STING pathway and its induction of autophagy play a crucial role in immune defense response against pathogenic DNA, but excessive activation of STING can exacerbate organ damage. Increased circulating mtDNA levels and STING activation in sALI patients contribute to lung injury by triggering an inflammatory response and disrupting autophagy. STING activation interferes with lysosomal acidification in an IFN-dependent manner, worsening sepsis.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Fan Zhao, Tao Zheng, Wenbin Gong, Jie Wu, Haohao Xie, Weijie Li, Rui Zhang, Peizhao Liu, Juanhan Liu, Xiuwen Wu, Yun Zhao, Jianan Ren
Summary: Exosomal dsDNA was found to promote intestinal inflammation via activating the STING pathway in macrophages, and STING deficiency significantly ameliorated colitis. The inhibitor GW4869 showed potential therapeutic effects by inhibiting EVs release in colitis.
CELL DEATH & DISEASE
(2021)
Article
Materials Science, Multidisciplinary
Canwen Chen, Yuetong Wang, Dagan Zhang, Xiuwen Wu, Yun Zhao, Luoran Shang, Jianan Ren, Yuanjin Zhao
Summary: This study introduced a multicompartment polysaccharide core-shell microparticle system for enhancing wound healing, exhibiting excellent antibacterial properties and angiogenesis effects.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Applied
Rong Yang, Jinjian Huang, Wenjie Zhang, Wenliang Xue, Yungang Jiang, Sicheng Li, Xiuwen Wu, Hong Xu, Jianan Ren, Bo Chi
Summary: Injectable hydrogels with tunable stiffness were developed to regulate fibroblast migration and infiltration. The hydrogel exhibited good biocompatibility, biodegradability, and self-healing property in in vitro studies. Animal experiments demonstrated its ability to promote wound healing by increasing collagen deposition and vascularization.
CARBOHYDRATE POLYMERS
(2021)
Review
Materials Science, Multidisciplinary
Jinjian Huang, Shaojun Xia, Zongan Li, Xiuwen Wu, Jianan Ren
Summary: Four-dimensional (4D) printing technology extends the capabilities of three-dimensional (3D) printing by enabling dynamic changes in shape over time. The technology has seen initial progress in various industrial fields, and ongoing research and breakthroughs point to a wide range of potential applications in the future.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2021)
Article
Infectious Diseases
Jie Wu, Mengxuan Li, Qinjie Liu, Xinjian Xu, Xiuming Lu, Gang Ma, Chi Ma, Xiaocheng Zhu, Xiuwen Wu, Jianan Ren
Summary: This study describes and analyzes the current status of the diagnosis and management of acute appendicitis in China. Non-operative management (NOM) is considered a viable treatment option for non-complicated acute appendicitis with lower complication rates and reasonable success rates, while not recommended for complicated cases due to higher complication rates and lower success rates.
SURGICAL INFECTIONS
(2021)
Correction
Cell Biology
Fan Zhao, Tao Zheng, Wenbin Gong, Jie Wu, Haohao Xie, Weijie Li, Rui Zhang, Peizhao Liu, Juanhan Liu, Xiuwen Wu, Yun Zhao, Jianan Ren
CELL DEATH & DISEASE
(2021)
Review
Immunology
Yangguang Li, Wenbin Gong, Weizhen Li, Peizhao Liu, Juanhan Liu, Haiyang Jiang, Tao Zheng, Jie Wu, Xiuwen Wu, Yun Zhao, Jianan Ren
Summary: This article reviews the activation factors of the IRG1-Itaconate axis and its role in regulating the inflammatory pathway in macrophages. It also summarizes the relationship between the axis and the metabolism of macrophages. Furthermore, it highlights the connections between the IRG1-Itaconate axis and inflammatory diseases.
INTERNATIONAL REVIEWS OF IMMUNOLOGY
(2023)
Article
Cell Biology
Jie Wu, Qinjie Liu, Xufei Zhang, Miaomiao Tan, Xuanheng Li, Peizhao Liu, Lei Wu, Fan Jiao, Zhaoyu Lin, Xiuwen Wu, Xin Wang, Yun Zhao, Jianan Ren
Summary: The mechanism by which STING causes lethal sepsis through inducing macrophage ferroptosis in an interferon-independent manner is explored. The interaction between STING and NCOA4 is found to be critical in this process. The selective 20-HETE synthase inhibitor, HET0016, is shown to reduce STING-induced ferroptosis and mortality in septic mice. These findings provide insights into sepsis and potential therapeutic targets.
CELL DEATH & DISEASE
(2022)
Article
Gastroenterology & Hepatology
Wenbin Gong, Kun Guo, Tao Zheng, Haohao Xie, Weijie Li, Mengxuan Li, Zhiwu Hong, Huajian Ren, Guosheng Gu, Gefei Wang, Xiuwen Wu, Jianan Ren
Summary: The JINLING score was found to be significantly correlated with FCP, CRP, and hemoglobin. In the validation cohort, patients with high inflammation grade (JINLING >= 4) had higher GHAS, CRP, and FCP than low inflammation grade patients. High JINLING score was associated with an increased risk of treatment failure.
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
(2021)
Article
Chemistry, Physical
Jinjian Huang, Ye Liu, Xiang Chi, Yungang Jiang, Ziyan Xu, Guiwen Qu, Yun Zhao, Zongan Li, Canwen Chen, Guopu Chen, Xiuwen Wu, Jianan Ren
Summary: This study developed an electronic skin with antibacterial, color-variable, UV-sensitive, shape-adaptive, and immunoregulatory functions, which can simulate the comprehensive functions of real skin. By combining single- and dual-network hydrogel structures with chemical reactions, it achieved the perception of UV and human motions.
JOURNAL OF MATERIALS CHEMISTRY A
(2021)
