Review
Chemistry, Multidisciplinary
Norraseth Kaeokhamloed, Samuel Legeay, Emilie Roger
Summary: Advanced drug delivery system using nanocarriers is a major application of nanotechnology in pharmacotherapeutics. However, the clinical translation of nanomedicine faces challenges, and this review focuses on utilizing Fo•rster resonance energy transfer (FRET) as a method to track intact nanocarriers in vivo for studying pharmacokinetics.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biochemical Research Methods
Agnes Batta, Timea Hajdu, Peter Nagy
Summary: Forster resonance energy transfer (FRET) is a radiationless interaction used to study protein oligomerization and structure with distance dependence. This study presents a method that improves FRET measurement precision by using microbeads with a calibrated number of antibody binding sites and a donor-acceptor mixture. The proposed method demonstrates superior reproducibility compared to the conventional approach and can be widely applied for the quantification of FRET experiments in biological research.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Polymer Science
Agustin Maceda, Teresa Terrazas
Summary: Various methods for analyzing lignin autofluorescence have been developed, including fluorescence microscopy and other semi-quantitative methods, which efficiently characterize lignin. These methods can be used to analyze the transport of lignin monomers, distribution of lignin types in different plant tissues, changes in wood degradation, and purity of cellulose nanofibers in lignocellulosic biomass.
Article
Engineering, Electrical & Electronic
Damian Tohl, Meseret N. Teferra, Angus Wallace, Anh Tran Tam Pham, Youhong Tang
Summary: Point-of-care (POC) medical devices have gained popularity in diagnosing and monitoring health conditions without specialized staff or attendance at medical centers. However, the accuracy and repeatability of light intensity-based measurements are affected by variations within and between devices. To address this, a universal method for device calibration and ratiometric measurement is presented, providing a robust measurement in different light intensity measurement devices.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Horticulture
Subhankar Mandal, Christopher S. Cramer
Summary: This study successfully quantified the development of FBR symptoms on onion bulbs' basal plate using digital image analysis and developed a relatively accurate automatic image segmentation method.
Article
Biochemistry & Molecular Biology
Nawar Sakr, Olga Glazova, Liudmila Shevkova, Nikita Onyanov, Samira Kaziakhmedova, Alena Shilova, Maria V. Vorontsova, Pavel Volchkov
Summary: Tissue autofluorescence in fixed tissue sections is a major issue in fluorescence microscopy. The intense intrinsic fluorescence emitted by the adrenal cortex interferes with fluorescent labels, resulting in poor-quality images. Various tissue treatment methods were evaluated for reducing autofluorescence, and TrueBlack (TM) Lipofuscin Autofluorescence Quencher and MaxBlock (TM) Autofluorescence Reducing Reagent Kit were found to be the most effective, reducing autofluorescence intensity by 89-93% and 90-95%, respectively. These treatments preserved specific fluorescence signals and tissue integrity, allowing reliable detection of fluorescent labels in the adrenal cortex tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Keith E. Duncan, Kirk J. Czymmek, Ni Jiang, August C. Thies, Christopher N. Topp
Summary: This study presents technical advances in using lab-based X-ray microscopy for high-resolution 3D imaging of plant samples at multiple scales. Serial imaging and improved sample preparation methods allow for the generation of sub-micron 3D volumes co-registered with lower magnification scans, providing explicit contextual reference. This method bridges the imaging gap between light and electron microscopy and can be applied to various economically and scientifically important plant systems.
Article
Chemistry, Physical
Cecilia Zaza, German Chiarelli, Ludovit P. Zweifel, Mauricio Pilo-Pais, Evangelos Sisamakis, Fabio Barachati, Fernando D. Stefani, Guillermo P. Acuna
Summary: Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions and functional states. However, conventional FLIM and FRET imaging provide average information over an ensemble of molecules within a diffraction-limited volume, which limits the spatial information, accuracy, and dynamic range of the observed signals. Here, an approach to obtain super-resolved FRET imaging based on single-molecule localization microscopy using an early prototype of a commercial time-resolved confocal microscope is demonstrated. DNA Points Accumulation for Imaging in Nanoscale Topography with fluorogenic probes provides a suitable combination of background reduction and binding kinetics compatible with the scanning speed of usual confocal microscopes. A single laser is used to excite the donor, a broad detection band is employed to retrieve both donor and acceptor emission, and FRET events are detected from lifetime information.
Article
Chemistry, Physical
Pratibha Agarwala, Turban Bera, Dibyendu K. Sasmal
Summary: This study reports the mechanism of interaction between curcumin and bovine serum albumin (BSA) as well as live E. coli cell membrane. The results show the formation of complexes between curcumin and BSA and the bacterial membrane, indicating a strong hydrophobic interaction. These findings shed light on the potential applications of curcumin as a natural therapeutic lead in clinical trials against life-threatening diseases.
Article
Materials Science, Multidisciplinary
Evgenii Vasilev, Jie Wang, Marko Knezevic
Summary: This study introduces a new metric to quantitatively assess fracture surfaces, successfully distinguishing between fracture in fatigue and fracture in monotonic tension. The metric is also sensitive to the ductility of tested alloys and the presence of critical defects.
MATERIALS CHARACTERIZATION
(2022)
Article
Engineering, Environmental
David Wertheim, Lisa Miyashita, Gary Foley, Ian Gill, Richard Giddens, Gavin Gillmore, Ruaraidh Dobson, Sean Semple, Ana Rule, Jonathan Grigg
Summary: Using confocal microscopy, this study examined the three-dimensional appearances and elemental characteristics of particulate matter found in the air at roadside and underground railway stations. The results showed that some particles have sharp surfaces, and the particles at the railway station primarily consist of iron, calcium, and silicon.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2023)
Review
Immunology
Anika Gruneboom, Oliver Aust, Zuelal Cibir, Flora Weber, Dirk M. Hermann, Matthias Gunzer
Summary: Innate immune cells play a crucial role in defense against infections and sterile inflammation, with recent advances in microscopy providing insights into their biology. Neutrophils and macrophages have protective roles during tumor growth and ischemia-reperfusion damage, but can also exacerbate diseases. Future directions include enhancing knowledge about innate immune cells and their potential applications in disease diagnostics.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Biology
Kathrin Malfeld, Nina Armbrecht, Holger A. Volk, Thomas Lenarz, Verena Scheper
Summary: Recent research has found that auditory synaptopathies play a crucial role in sensorineural hearing loss, and cochlear implant procedures may lead to physiological hearing loss in patients with residual hearing. The study developed a new method to examine the inner ear at the synapse level, aiming to better understand the cascade of hearing pathologies with the goal of developing targeted therapeutics.
Article
Environmental Sciences
Kat Austen, Joana MacLean, Daniel Balanzategui, Franz Hoelker
Summary: Microplastic beads tagged with fluorescent dye were introduced into the soil of potted silver birch saplings, and after five months, the presence of microplastic in root tissues was confirmed. This study suggests the potential of woody plants like birch in phytoremediation of soil contaminated with microplastic.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Agnes Angyal, Zsofia Penzes, Shahrzad Alimohammadi, Dorottya Horvath, Lili Takacs, Gyorgy Vereb, Barbara Zsebik, Tamas Biro, Kinga Fanni Toth, Erika Lisztes, Balazs Istvan Toth, Attila Olah, Attila Gabor Szollosi
Summary: The study found that human corneal epithelial cells express members of the endocannabinoid system, playing a role in inflammatory responses. Despite previous reports on the anti-inflammatory potential of endocannabinoids, their effects on the immune phenotype of human corneal epithelium may be more complex and context dependent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Lisa Maria Mustachio, Jason Roszik
Summary: Single-cell sequencing technologies have become increasingly important in cancer research, allowing scientists to analyze the genome, transcriptome, epigenome, and proteome of individual cells. This review summarizes the various techniques used in single-cell sequencing and provides examples of recent high-impact findings. It also discusses the translational relevance of these technologies and their potential use in clinical trials.
Article
Oncology
Jason Roszik, Lisa Maria Mustachio, John A. Livingston, Roman Groisberg, Roberto Carmagnani Pestana, Vivek Subbiah, Anthony P. Conley
Summary: Soft tissue sarcomas, rare diseases of mesenchymal origin, display heterogeneity and varying clinical behaviors, making standardized therapy difficult. Studies show high potential of immunotherapy for sarcoma treatment, especially in subtypes with specific genomic alterations and immune cell markers. Sarcomas with high mutational load may be more sensitive to immunotherapy, with potential for developing optimal treatments based on immune composition.
Article
Oncology
Caitlyn B. Brashears, Bethany C. Prudner, Richa Rathore, Katharine E. Caldwell, Carina A. Dehner, Jane L. Buchanan, Sara E. S. Lange, Neal Poulin, Jennifer K. Sehn, Jason Roszik, Dirk Spitzer, Kevin B. Jones, Regis O'Keefe, Torsten O. Nielsen, Eric B. Taylor, Jason M. Held, William Hawkins, Brian A. Van Tine
Summary: The study investigates the metabolism of synovial sarcoma (SS) and highlights the impact of malic enzyme 1 absence on SS redox homeostasis. The findings suggest that targeting redox homeostasis in ME1-null cancers could be a potential therapeutic approach, with increased susceptibility to ferroptosis induction. These results provide a preclinical rationale for conducting a phase I trial of ACXT-3102 in SS patients.
CLINICAL CANCER RESEARCH
(2022)
Review
Oncology
Jacob J. Adashek, Arjun K. Menta, Neha K. Reddy, Aakash P. Desai, Jason Roszik, Vivek Subbiah
Summary: BRAF V600 mutations are prevalent in various nonmelanoma malignancies and can be clinically targeted with BRAF inhibitor therapy, suggesting their potential application in the treatment of nonmelanoma rare malignancies in both adults and children.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Oncology
Fan Fan, Jason Roszik, Ling Xia, Susmita Ghosh, Rui Wang, Xiangcang Ye, David Hawke, Lee M. Ellis, Rajat Bhattacharya
Summary: This study demonstrates the effects of depleting CYTSA protein on the proliferation and migration of colorectal cancer cells, suggesting that targeting CYTSA may be a novel therapeutic strategy for CRC.
Article
Oncology
Mihaly Cserepes, Gyorgyi A. Nelhubel, Monika Meilinger-Dobra, Adrienn Herczeg, Dora Turk, Zita Hegedus, Laura Svajda, Erzsebet Raso, Andrea Ladanyi, Kristof Gyorgy Csiko, Istvan Kenessey, Arpad Szoor, Gyorgy Vereb, Eva Remenar, Jozsef Tovari
Summary: In this study, the effects of EGFRvIII and EGFR R521K variants on cetuximab efficacy were investigated. The results showed that the frequency of the EGFRvIII allele in head and neck squamous cell carcinoma patients is less than 1%, indicating that it does not lead to common resistance. EGFR R521K, present in 42% of the patients, was found to reduce cell sensitivity in vitro and in vivo experiments, but this preclinical difference did not correlate with patients' progression-free or overall survival.
Article
Biochemical Research Methods
Istvan Rebenku, Ferenc A. Bartha, Tamas Katona, Barbara Zsebik, Geza Antalffy, Lili Takacs, Bela Molnar, Gyorgy Vereb
Summary: The emergence and fast advance of digital pathology allows the acquisition, digital storage, interactive recall and analysis of tissue-level morphology. The rise of fluorescence pathology scanners expands the detection of molecules based on multiplex labeling. The Pannoramic Confocal provides sensitive, quantitative widefield and confocal detection of multiplexed fluorescence signals, with optical sectioning and 3D reconstruction.
Article
Oncology
Justin T. Moyers, Roberto Carmagnani Pestana, Jason Roszik, David S. Hong, Aung Naing, Siqing Fu, Sarina Piha-Paul, Timothy A. Yap, Daniel Karp, Jordi Rodon, Andy Livingston, Maria Alejandra Zarzour, Vinod Ravi, Shreyaskumar Patel, Robert S. Benjamin, Joseph Ludwig, Cynthia Herzog, Ravin Ratan, Neeta Somaiah, Anthony Conley, Richard Gorlick, Funda Meric-Bernstam, Vivek Subbiah
Summary: In this study, the outcomes of patients with ultrarare sarcomas in Phase 1 trials were assessed. The results showed that the median overall survival of ultrarare sarcomas was similar to common sarcomas, but the objective response rate to treatment was higher. Genomic selection played a significant role in identifying molecular subsets likely to benefit from targeted therapy in Phase 1 trials.
CLINICAL CANCER RESEARCH
(2023)
Article
Oncology
Chao-Hsien Chen, Renee L. Chin, Genevieve P. Hartley, Spencer T. Lea, Brian J. Engel, Cheng-En Hsieh, Rishika Prasad, Jason Roszik, Takashi Shingu, Gregory A. Lizee, Amy B. Heimberger, Steven W. Millward, Jian Hu, David S. Hong, Michael A. Curran
Summary: This study developed murine glioblastoma stem cell lines with clinically relevant genetic mutations and evaluated their immune sensitivities in immunocompetent hosts. The results showed that different cell lines have varying sensitivities to T cell immune checkpoint blockade, and there are differences between the brain and flank. Immunological analysis revealed that upregulation of PD-L1 on myeloid stroma establishes immune privilege in the brain. However, QPP7 cell line showed response to immunotherapy even in the brain due to its elevated neoantigen burden. These mouse models can be used for mechanistic discovery and evaluation of immune therapies.
Article
Oncology
Chandrani Chattopadhyay, Rajat Bhattacharya, Jason Roszik, Fatima S. Khan, Gabrielle A. Wells, Hugo Villanueva, Yong Qin, Rishav Bhattacharya, Sapna P. Patel, Elizabeth A. Grimm
Summary: Researchers found that inhibiting the IGF-1 signaling pathway can reduce cell survival and migration, while promoting apoptosis in UM. In vivo models also showed a decrease in tumor growth with this treatment.
Article
Oncology
Susmita Ghosh, Fan Fan, Reid T. Powell, Jason Roszik, Yong Sung Park, Clifford Stephan, Manu Sebastian, Lin Tan, Alexey Sorokin, Philip L. Lorenzi, Scott Kopetz, Lee M. Ellis, Rajat Bhattacharya
Summary: Mutations in KRAS are common in metastatic colorectal cancer (mCRC) and direct targeting of these mutations is difficult. In this study, we found that the combination of trametinib and vincristine showed significant efficacy in inhibiting tumor growth and inducing apoptosis in KRAS-mutant colorectal cancer. The combination also inhibited the RAS-RAF-MEK and PI3K-AKT-mTOR survival pathways.
MOLECULAR CANCER THERAPEUTICS
(2023)
Article
Oncology
Yasunari Fukuda, Sun-Hee Kim, Matias A. Bustos, Sung-Nam Cho, Jason Roszik, Jared K. Burks, Hong Kim, Dave S. B. Hoon, Elizabeth A. Grimm, Suhendan Ekmekcioglu
Summary: The arachidonic acid pathway, specifically microsomal prostaglandin E2 synthase 1 (mPGES-1), plays important roles in regulating tumor immunity in melanoma. The study suggests that inhibiting mPGES-1 can reduce collagen deposition and T-cell exhaustion, thereby reinforcing immunotherapy in melanoma.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Kyaw Z. Thein, Amadeo B. Biter, Kimberly C. Banks, Andrew W. Duda, Jennifer Saam, Jason Roszik, Filip Janku, Ferdinandos Skoulidis, John V. Heymach, Scott Kopetz, Funda Meric-Bernstam, David S. Hong
Summary: This study conducted a comprehensive analysis of KRAS(G12C) mutations in different types of cancer through liquid biopsy. The results showed that KRAS(G12C) mutations were detected in various cancers, with the highest detection rate in lung cancer.
JCO PRECISION ONCOLOGY
(2022)
Article
Oncology
Jason Roszik, J. Jack Lee, Yi-Hung Wu, Xi Liu, Masanori Kawakami, Jonathan M. Kurie, Anas Belouali, Simina M. Boca, Samir Gupta, Robert A. Beckman, Subha Madhavan, Ethan Dmitrovsky
Summary: This study investigates whether NSAIDs improve overall survival in non-small cell lung cancer (NSCLC) through real-world studies and natural language processing (NLP). The results show a statistically significant association between NSAID use and increased NSCLC survival across different stages, histopathology, gender, smoking history, and demographic groups. These findings provide a rationale for future randomized trials of NSAIDs in NSCLC.
CANCER RESEARCH COMMUNICATIONS
(2022)
