Article
Chemistry, Physical
Cecilia Zaza, German Chiarelli, Ludovit P. Zweifel, Mauricio Pilo-Pais, Evangelos Sisamakis, Fabio Barachati, Fernando D. Stefani, Guillermo P. Acuna
Summary: Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions and functional states. However, conventional FLIM and FRET imaging provide average information over an ensemble of molecules within a diffraction-limited volume, which limits the spatial information, accuracy, and dynamic range of the observed signals. Here, an approach to obtain super-resolved FRET imaging based on single-molecule localization microscopy using an early prototype of a commercial time-resolved confocal microscope is demonstrated. DNA Points Accumulation for Imaging in Nanoscale Topography with fluorogenic probes provides a suitable combination of background reduction and binding kinetics compatible with the scanning speed of usual confocal microscopes. A single laser is used to excite the donor, a broad detection band is employed to retrieve both donor and acceptor emission, and FRET events are detected from lifetime information.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Nanoscience & Nanotechnology
Yuan Luo, Ming Lun Tseng, Sunil Vyas, Ting-Yu Hsieh, Jui-Ching Wu, Shang-Yang Chen, Hsiao-Fang Peng, Vin-Cent Su, Tzu-Ting Huang, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Jia-Wern Chen, Yu-Chun Chen, Kuang-Yuh Huang, Chieh-Hsiung Kuan, Xu Shi, Hiroaki Misawa, Din Ping Tsai
Summary: Light-sheet fluorescent microscopy (LSFM) is the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. This study demonstrates the successful integration of a nanophotonic meta-lens as the illumination component for LSFM, addressing the challenges of high image resolution and optical sectioning. With the meta-lens, the LSFM system complexity is significantly reduced, enabling multicolor fluorescent imaging of live biological specimens with cellular resolution.
Article
Urology & Nephrology
Jesus Z. Villarreal, Javiera Perez-Anker, Susana Puig, Marc Xipell, Gerard Espinosa, Esther Barnadas, Ana B. Larque, J. Malvehy, Ricard Cervera, Arturo Pereira, Antonio Martinez-Pozo, Luis F. Quintana, Adriana Garcia-Herrera
Summary: This study demonstrates the feasibility of using ex vivo confocal microscopy for the analysis of basic patterns of lesion in nephropathology. FuCM proves to be as effective as conventional techniques in evaluating acute tubular necrosis and interstitial fibrosis changes, facilitating rapid evaluation of kidney biopsies and restructuring the clinical workflow in renal histopathology.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Urology & Nephrology
Jesus Z. Villarreal, J. Perez-Anker, S. Puig, G. Pellacani, M. Sole, J. Malvehy, Luis F. Quintana, A. Garcia-Herrera
Summary: This study evaluated the feasibility of using ex vivo confocal microscopy in non-neoplastic kidney biopsies, and found that confocal images correlated well with conventional histological sections without introducing artifacts to the samples. The ease and speed of image acquisition, along with the quality of digitally stained images obtained, suggest promise for clinical applications in nephrology and renal transplantation.
JOURNAL OF NEPHROLOGY
(2021)
Article
Chemistry, Multidisciplinary
Guankui Wang, Markella Zannikou, Laren Lofchy, Yue Li, Hanmant Gaikwad, Irina Balyasnikova, Dmitri Simberg
Summary: The type of fluorescent label on liposomes significantly impacts their extravasation and accumulation in tumors. In animal experiments, indocarbocyanine lipids (ICLs) were found to spread and accumulate in tumor tissue more easily compared to fluorescent phospholipids (FPLs).
Article
Chemistry, Multidisciplinary
Wenbo Zhang, Luca Bertinetti, Kerstin G. Blank, Rumiana Dimova, Changyou Gao, Emanuel Schneck, Peter Fratzl
Summary: The study introduces a novel osmotic pressure sensor based on liposomes loaded with water-soluble fluorescent dyes exhibiting resonance energy transfer. These sensors show high sensitivity to osmotic pressure in the biologically relevant range and can be used for investigating osmotic pressure dynamics in biology and technology using FRET microscopy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Anayat Bhat, Shuang Li, Daniel Hammler, Martin J. Winterhalder, Andreas Marx, Andreas Zumbusch
Summary: The hydrolysis of nucleotides is important for cellular energy, and the transfer of phosphates to proteins is crucial for post-translational modification. A novel fluorogenic adenosine 5'-tetraphosphate (Ap4) analog has been developed for real-time monitoring of ATP hydrolysis within live cells, with potential applications in studying cellular processes involving Ap4 hydrolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Jun-Fang Wang, Chun-Miao Zhao, Jian-Xing Yang, Xu He, Xiao-Liu Li, Jin-Mei Li, Ke-Rang Wang
Summary: A mannose-modified perylene monoimide derivative PMI-Man has been developed, which exhibits highly selective binding to double-stranded DNA molecules, potent live/dead cell imaging, and histological imaging via both confocal and light microscopies. This approach may be utilized to create a universal colorful staining method for human tissues suitable for both confocal and light microscopies.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Pratibha Agarwala, Turban Bera, Dibyendu K. Sasmal
Summary: This study reports the mechanism of interaction between curcumin and bovine serum albumin (BSA) as well as live E. coli cell membrane. The results show the formation of complexes between curcumin and BSA and the bacterial membrane, indicating a strong hydrophobic interaction. These findings shed light on the potential applications of curcumin as a natural therapeutic lead in clinical trials against life-threatening diseases.
Article
Medicine, General & Internal
Veronika Shavlokhova, Christa Flechtenmacher, Sameena Sandhu, Michael Vollmer, Andreas Vollmer, Maximilian Pilz, Juergen Hoffmann, Oliver Ristow, Michael Engel, Christian Freudlsperger
Summary: The study demonstrates the potential of ex vivo fluorescence confocal microscopy in providing rapid real-time assessment of oral pathologies, showing high sensitivity and specificity in detecting oral leukoplakia.
Review
Pharmacology & Pharmacy
Jeffrey Momoh, Dimitrios Kapsokalyvas, Michael Vogt, Sjoerd Hak, Fabian Kiessling, Marc van Zandvoort, Twan Lammers, Alexandros Marios Sofias
Summary: Intravital microscopy (IVM) has broad applications in various fields, including biology, physiology, immunology, and drug development. This review provides an overview of the modalities, methods, and model organisms used in IVM, as well as its applications in nanomedicine and biological process studies.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Biochemical Research Methods
Shan Gao, Mengzhou Li, Jason T. Smith, Xavier Intes
Summary: We report on a novel time-domain mesoscopic fluorescence molecular tomography (TD-MFMT) system designed for multiplexed molecular imaging in turbid media, demonstrating high sensitivity and specificity. Phantom studies and ex vivo tumor imaging validate the system's potential for imaging deep targets and resolving intra-tumoral heterogeneity.
BIOMEDICAL OPTICS EXPRESS
(2022)
Review
Immunology
Anika Gruneboom, Oliver Aust, Zuelal Cibir, Flora Weber, Dirk M. Hermann, Matthias Gunzer
Summary: Innate immune cells play a crucial role in defense against infections and sterile inflammation, with recent advances in microscopy providing insights into their biology. Neutrophils and macrophages have protective roles during tumor growth and ischemia-reperfusion damage, but can also exacerbate diseases. Future directions include enhancing knowledge about innate immune cells and their potential applications in disease diagnostics.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Carmelo Puglia, Debora Santonocito, Giuseppe Romeo, Sebastiano Intagliata, Giovanni Luca Romano, Enrica Strettoi, Elena Novelli, Carmine Ostacolo, Pietro Campiglia, Eduardo Maria Sommella, Rosario Pignatello, Claudio Bucolo
Summary: Lipid-based nanocarriers have been developed to improve the bioavailability of ophthalmic drugs, with a novel formulation LN-ODAF showing promising potential in diffusing through different ocular compartments and reaching the back of the eye.
Review
Pharmacology & Pharmacy
Thomas Briot, Emilie Roger, Sylvain Thepot, Frederic Lagarce
DRUG DISCOVERY TODAY
(2018)
Article
Nanoscience & Nanotechnology
Thomas Briot, Emilie Roger, Naila Bou Haidar, Jerome Bejaud, Nolwenn Lautram, Catherine Guillet, Sylvain Thepot, Samuel Legeay, Frederic Lagarce
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2019)
Review
Nanoscience & Nanotechnology
Anne Clavreul, Milad Pourbaghi-Masouleh, Emilie Roger, Philippe Menei
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2019)
Article
Chemistry, Physical
Florence Dumas, Emilie Roger, Johanna Rodriguez, Lazhar Benyahia, Jean-Pierre Benoit
COLLOID AND POLYMER SCIENCE
(2020)
Article
Pharmacology & Pharmacy
Norraseth Kaeokhamloed, Emillie Roger, Jerome Bejaud, Nolwenn Lautram, Florence Manero, Rodolphe Perrot, Chadi Abbara, Marie Briet, Samuel Legeay
Summary: The study developed a coculture model of Caco-2 epithelium and HMEC-1 for evaluating the intestinal absorption of drugs and nanoparticles. Surface modification was found to alter the absorption mechanism of lipid nanocapsules.
Article
Chemistry, Physical
Florence Dumas, Jean-Pierre Benoit, Patrick Saulnier, Emilie Roger
Summary: A novel method for stabilizing ATPS was proposed by adsorbing semi-solid particles at the interface between the two aqueous phases and forming microparticles with a gelled shell through polyelectrolyte complexation. This approach improved the stability and long-term stability of ATPS, making it possible to use them for delivery systems encapsulating hydrophilic molecules.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
Samuel Bonnet, Rana Elfatairi, Florence Franconi, Emilie Roger, Samuel Legeay
Summary: Studying the pharmacokinetics (PK) of NPs in vivo is crucial, and among the various tracking methods, Forster resonance energy transfer (FRET) shows great potential for tracking NPs' integrity. However, validated methods are still needed for extracting and quantifying NPs in biological fluids and tissues.
Article
Pharmacology & Pharmacy
Velaphi C. Thipe, Alice Raphael Karikachery, Pinar Cakilkaya, Umer Farooq, Hussein H. Genedy, Norraseth Kaeokhamloed, Dieu-Hien Phan, Refaya Rezwan, Gozde Tezcan, Emilie Roger, Kattesh Katti
Summary: This review highlights recent advances in green nanotechnology, particularly in the synthesis of biocompatible and medically relevant gold nanoparticles. It provides detailed information on synthetic protocols and characterization tools for gold nanoparticles, as well as their applications in diagnostic imaging, drug delivery, and cancer therapy. The review also emphasizes the importance of green processes and the role of nanomedicine in human health and hygiene.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Vincent Lebreton, Norraseth Kaeokhamloed, Anastasiia Vasylaki, Gregory Hilairet, Adelie Mellinger, Jerome Bejaud, Patrick Saulnier, Frederic Lagarce, Florence Gattacceca, Samuel Legeay, Emilie Roger
Summary: This study investigated the pharmacokinetics of intact lipid nanocapsules after intravenous administration in rats, revealing that the elimination of FRET-LNCs was non-linear and dependent on size and surface modification, while the distribution was dependent on size. LNCs with PEG coating displayed a lower clearance than other formulations.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Pharmacology & Pharmacy
Anne Clavreul, Emilie Roger, Milad Pourbaghi-Masouleh, Laurent Lemaire, Clement Tetaud, Philippe Menei
Review
Chemistry, Multidisciplinary
Zhiyuan Shi, Yong Hu, Xin Li
Summary: Controlled drug delivery systems that can respond to mechanical force offer a unique solution for on-demand drug activation and release. Among various mechanical stimuli, ultrasound (US) has advantages in achieving spatiotemporally controlled drug release. Traditional US-triggered drug release relies on heat-induced phase transitions or chemical transformations, while the cutting-edge approach of Sonopharmacology leverages polymer mechanochemistry. The remaining challenges and potential future directions in this field are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Lijie Zheng, Yuanzheng Chen, Xun Gu, Yingying Li, Hanqing Zhao, Wenjun Shao, Tao Ma, Chuanbin Wu, Qingqing Wang
Summary: In this study, a novel dosage form consisting of dissolving microneedles and an adhesive transdermal patch was developed for the treatment of rheumatoid arthritis. In vitro and in vivo experiments demonstrated that the combination of drugs delivered by this dosage form effectively reduced joint inflammation and damage.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Kyungjik Yang, Hwa Seung Han, Seung Hwan An, Kyung Hoon Park, Keonwook Nam, Shinha Hwang, Yuyeon Lee, Sung Yeon Cho, Taehyung Kim, Deokyeong Choe, Sang Won Kim, Wonkyu Yu, Hyunah Lee, Jiyong Park, Sangguan You, Dong- Gyu Jo, Ki Young Choi, Young Hoon Roh, Jae Hyung Park
Summary: This study developed CP-loaded CS microcapsules to enhance the oral bioavailability of CP through controlled gastrointestinal delivery. The optimized microcapsules exhibited desirable physicochemical properties, showed anti-photoaging effects via antioxidant activity, and achieved controlled release in the gastrointestinal tract. This research provides a simple and economical approach for enhancing the oral bioavailability of CP for customized bioactive compound administration.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Huiyang Li, Shuo Liu, Wenjin Dai, Bingmei Yao, Yong Zhou, Sujia Si, Hairong Yu, Riguang Zhao, Fang Jin, Liqun Jiang
Summary: Changes in bodily fluid pressures are crucial in diseases like high-altitude pulmonary edema (HAPE). Researchers have developed hydrostatic pressure-sensitive multivesicular liposomes (PSMVLs) that can release drugs in response to pressure changes, with potential applications in HAPE treatment. Animal experiments showed that this system provides better protection for lung tissues and respiratory function, reducing the occurrence of pulmonary edema.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Qian Hu, Hongbing Lan, Yinmei Tian, Xiaonan Li, Mengmeng Wang, Jiao Zhang, Yulin Yu, Wei Chen, Li Kong, Yuanyuan Guo, Zhiping Zhang
Summary: Coacervate droplets formed through liquid-liquid phase separation have potential as delivery vesicles for therapeutics. However, their lack of physiological stability and membranes are challenges. In this study, polylysine-polynucleotide complex coacervate droplets with favorable stability were formulated to concentrate molecules and nanoparticles. Phospholipid membranes were further coated on the droplets to create coacervate-based artificial protocells (ArtPC) with membrane-like structures. These biofunctional ArtPC effectively reduced blood uric acid levels and prevented renal injuries.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Xiaowei Wang, Hongwei Lu, Fang Luo, Dan Wang, Apeng Wang, Xuelei Wang, Wenkai Feng, Xiaobo Wang, Jiayi Su, Mingliang Liu, Guimin Xia
Summary: Four novel lipid-like GEM diesters were synthesized and encapsulated into liposomes to improve the antitumor efficacy of Gemcitabine. The liposomes loaded with dimyristoyl GEM (LipodmGEM) showed enhanced cellular uptake, improved inhibition of cell migration, and a greatly extended half-life compared to free Gemcitabine. LipodmGEM successfully enriched the drug in the tumor and exhibited excellent anticancer efficacy in vivo with negligible systemic toxicity.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Yajing Sun, Ze Lu, John A. Taylor, Jessie L. S. Au
Summary: A recent development in cancer chemotherapy is the use of cytotoxics to induce tumor-specific immune response through immunogenic cell death (ICD). This study describes a method that utilizes immunostaining and machine-learning to identify cells with ecto-CRT in intact 3-dimensional tissues. The method was successfully applied to study drug-induced ICD in human bladder cancer.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Chemistry, Multidisciplinary
Rafat Ali, Shantanu Sen, Rohil Hameed, Aamir Nazir, Sandeep Verma
Summary: This review provides a focused overview of emerging strategies for delivering gasotransmitters in a controlled and sustained manner to reestablish neurophysiological homeostasis.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Jing Chen, Xinyi Zhang, Jinshen Zhang, Zhaoxia Wang, Guilan Zhu, Ming Geng, Jinmiao Zhu, Yajun Chen, Wei Wang, Youcui Xu
Summary: In this study, a multifunctional responsive hydrogel system was developed for synergistic reoxygenation and chemo/photothermal therapy. The hydrogel system showed both therapeutic effects against metastatic breast cancer and wound infection, making it a promising strategy for treating and preventing tumor recurrence and associated wound infection.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Baoshan Huang, Na Zhang, Xinying Qiu, Rui Zeng, Shuimiao Wang, Mengxia Hua, Qing Li, Kaihui Nan, Sen Lin
Summary: This study revealed that robust ROS can oxidize mitochondrial DNA (ox-mtDNA) and cause its release into the cytosol, resulting in the activation of NLRP3 inflammasome. By using the mitochondria-targeted antioxidant SkQ1 and a novel mitochondria-targeted nanoparticle (SkQ1 NP), it was found that mitochondrial ROS scavenging could in situ inhibit DED-induced mtDNA oxidation and suppress NLRP3-mediated inflammation.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Wenqi Liu, Cheng Hu, Linyu Long, Shuyi He, Wen Zhang, Zhicun Wang, Li Yang, Yunbing Wang
Summary: Myocardial infarction is the leading cause of cardiovascular mortality, and current treatment methods have limitations. This study developed a smart carrier that can release different therapeutic substances for different pathological processes, effectively improving cardiac function, promoting cardiac repair, and preventing ventricular remodeling.
JOURNAL OF CONTROLLED RELEASE
(2024)