Article
Biochemistry & Molecular Biology
Nilufar Ali, Mukta S. Sane, Huiyuan Tang, Jadon Compher, Quinlan Mclaughlin, Christopher D. Jones, Shivani Kaushal Maffi
Summary: This study demonstrates that acute exposure to 6-hydroxydopamine (6-OHDA) activates multiple cell death pathways and has significant effects on the expression of mitochondrial complexes and apoptotic cell death cascade. The research provides a basis for further investigation into the impact of 6-OHDA on the ubiquitin proteasome system in PD therapies.
NEUROCHEMISTRY INTERNATIONAL
(2023)
Article
Neurosciences
Mareike Fauser, Manuel Ricken, Franz Markert, Nikolai Weis, Oliver Schmitt, Jan Gimsa, Christine Winter, Kathrin Badstuebner-Meeske, Alexander Storch
Summary: The study revealed that animals treated with STN-DBS had significantly increased numbers of dopamine neurons and fibers, potentially improving sensorimotor neglect. This therapy not only affected the nigrostriatal system, but also had a significant impact on the mesolimbic system.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Behavioral Sciences
Bita Firouzan, Farideh Iravanpour, Fatemeh Abbaszadeh, Valery Akparov, Jalal Zaringhalam, Rasoul Ghasemi, Nader Maghsoudi
Summary: This study investigated the effect of BHME on 6-OHDA-induced motor anomalies. Results showed that BHME prevented motor dysfunction and DA cell death following 6-OHDA injection, which was accompanied by an enhancement in Akt activity, decrement of P38 phosphorylation, and reduction in Bax/Bcl-2 ratio.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Review
Clinical Neurology
Richard M. Kostrzewa
Summary: Neonatal 6-OHDA-lesioned rats treated with desipramine can be used as a viable animal model for Parkinson's disease, as it accurately reproduces the dopaminergic neuronal destruction and the associated behavioral outcomes.
JOURNAL OF NEURAL TRANSMISSION
(2022)
Article
Cell Biology
Xin He, Yue Xie, Qiongping Zheng, Zeyu Zhang, Shanshan Ma, Junyu Li, Mingtao Li, Qiaoying Huang
Summary: The transcription factor TFE3 has been found to play a crucial role in maintaining autophagy and the survival of dopaminergic neurons in Parkinson's disease. Activation of TFE3 may serve as a promising strategy for PD therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Victor Silva da Fonseca, Valeria de Cassia Goncalves, Mario Augusto Izidoro, Antonio-Carlos Guimaraes de Almeida, Fernando Luiz Affonso Fonseca, Fulvio Alexandre Scorza, Josef Finsterer, Carla Alessandra Scorza
Summary: Parkinson's disease is an incurable neurodegenerative disease with an increasing global disability and mortality rate. Non-motor symptoms are the main cause of disability and decreased quality of life. This study used metabolomics to assess changes in cardiac metabolism in a PD model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuening Luo, Siqi Zhou, Reiko Takeda, Kazuma Okazaki, Marie Sekita, Kazuichi Sakamoto
Summary: In this study, amber extract was found to protect neuronal cells from apoptosis in a Parkinson's disease model by decreasing ROS levels and promoting autophagy.
Article
Hematology
Bryanna Reinhardt, Omar Habib, Kit L. Shaw, Elizabeth Garabedian, Denise A. Carbonaro-Sarracino, Dayna Terrazas, Beatriz Campo Fernandez, Satiro De Oliveira, Theodore B. Moore, Alan K. Ikeda, Barbara C. Engel, Gregory M. Podsakoff, Roger P. Hollis, Augustine Fernandes, Connie Jackson, Sally Shupien, Suparna Mishra, Alejandra Davila, Jack Mottahedeh, Andrej Vitomirov, Wenzhao Meng, Aaron M. Rosenfeld, Aoife M. Roche, Pascha Hokama, Shantan Reddy, John Everett, Xiaoyan Wang, Eline T. Luning Prak, Kenneth Cornetta, Michael S. Hershfield, Robert Sokolic, Suk See De Ravin, Harry L. Malech, Frederic D. Bushman, Fabio Candotti, Donald B. Kohn
Summary: Gene therapy for ADA SCID has shown long-term efficacy in immune reconstitution, with younger patients and those receiving higher doses of gene-marked CD34(+) cells generally having better outcomes. However, risks of genotoxicity with gammaretroviral vectors have also been highlighted.
Article
Multidisciplinary Sciences
Andrea Slezia, Panna Hegedus, Evgeniia Rusina, Katalin Lengyel, Nicola Solari, Attila Kaszas, Diana Balazsfi, Boris Botzanowski, Emma Acerbo, Florian Missey, Adam Williamson, Balazs Hangya
Summary: Studying animal models allows us to better understand the mechanisms of Parkinson's disease. However, current models mainly focus on late-stage symptoms, leaving a gap in our knowledge of early-stage changes. In this study, a graded-dose unilateral 6-OHDA model was established in mice, and the behavioral, neuronal, and ultrastructural features were analyzed within the first two weeks of lesion induction. The results showed that mild degeneration of dopaminergic neurons can lead to motor deficits shortly after striatal lesions, suggesting that this model can be used to study the early stages of Parkinson's disease in mice.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Nina I. Uspalenko, Alexei A. Mosentsov, Natalia V. Khmil, Lyubov L. Pavlik, Natalia V. Belosludtseva, Natalia V. Khunderyakova, Maria I. Shigaeva, Vasilisa P. Medvedeva, Anton E. Malkov, Valentina F. Kitchigina, Galina D. Mironova
Summary: Uridine has a protective effect on Parkinson's disease and activates the mitoK-ATP channel. It can restore mitochondrial damage, oxidative stress, and increased lactate dehydrogenase activity induced by PD. The protective effect of uridine is closely related to the mitoK-ATP channel.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Shu-Chun Chen, Hsi Chen, Seong-Jin Yu, Yun-Hsiang Chen, Yun Wang
Summary: This study aimed to examine the therapeutic effect of NAc lesioning by a selective dopaminergic toxin in Meth-sensitized animals. The results showed that Meth behavioral sensitization was significantly antagonized after the lesioning. Treatment with 6-OHDA significantly antagonized the upregulation of TH and Shati in the NAc. These findings suggest a new surgical treatment strategy for chronic Meth abuse.
CELL TRANSPLANTATION
(2021)
Article
Neurosciences
Ying Lyu, Yiying Huang, Guiying Shi, Xuepei Lei, Keya Li, Ran Zhou, Lin Bai, Chuan Qin
Summary: This study revealed gene expression changes in the five brain regions following exposure to 6-hydroxydopamine in PD rats, identifying key pathways and cascade involved in synaptic damage. The dopaminergic synapse, retrograde endocannabinoid signaling, and Gi/o-GIRK were highlighted as potential key pathways. Potential candidate genes such as Ephx2, Fam111a, and Gng2 were identified for further studies on the pathogenesis of PD.
CNS NEUROSCIENCE & THERAPEUTICS
(2021)
Article
Integrative & Complementary Medicine
Wen Jing, Zhang Yuan-Qiang, Liu Dong-Qing, Yao Xu-Tao, Jiang Hua, Zhang Yu-Bin
Summary: This study aimed to explore the neuroprotective effects and pharmacological mechanism of Demethylenetetrahydroberberine (DMTHB) on Parkinson's disease. The results showed that DMTHB could alleviate behavioral disorders, protect dopaminergic neurons, and exhibit multiple-target effects including anti-apoptotic, anti-inflammatory, and antioxidant properties.
CHINESE JOURNAL OF NATURAL MEDICINES
(2022)
Article
Immunology
Shuangjun Liu, Feng Huo, Guorui Dai, Jie Wu, Maoquan Qin, Huawei Mao, Quan Wang
Summary: This case report describes a patient who developed immune reconstitution inflammatory syndrome (IRIS) after hematopoietic stem cell transplantation, following severe disseminated infection caused by bacillus Calmette-Guerin (BCG) vaccine. The patient experienced complications including veno-occlusive disease and acute graft-versus-host disease post-transplant, with symptoms of IRIS emerging after immunosuppressants were withdrawn.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Leonie M. Heger, Rachel M. Wise, J. Tabitha Hees, Angelika B. Harbauer, Lena F. Burbulla
Summary: This review assesses mitochondrial phenotypes in iPSC-derived neurons from PD patients, focusing on the role of mitochondrial respiration, morphology, trafficking, mitophagy, and calcium handling in health and disease. It also summarizes the unique advantages and challenges of iPSC disease modeling for future mechanistic and therapeutic applications, highlighting the importance of studying mitochondrial dynamics in PD pathophysiology.
Article
Biochemistry & Molecular Biology
Koichi Oshima, Norikazu Saiki, Michihiro Tanaka, Hiromi Imamura, Akira Niwa, Ayako Tanimura, Ayako Nagahashi, Akiyoshi Hirayama, Keisuke Okita, Akitsu Hotta, Shuichi Kitayama, Mitsujiro Osawa, Shin Kaneko, Akira Watanabe, Isao Asaka, Wataru Fujibuchi, Kohsuke Imai, Hiromasa Yabe, Yoshiro Kamachi, Junichi Hara, Seiji Kojima, Masaru Tomita, Tomoyoshi Soga, Takafumi Noma, Shigeaki Nonoyama, Tatsutoshi Nakahata, Megumu K. Saito
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Review
Physiology
Peter Karagiannis, Kazutoshi Takahashi, Megumu Saito, Yoshinori Yoshida, Keisuke Okita, Akira Watanabe, Haruhisa Inoue, Jun K. Yamashita, Masaya Todani, Masato Nakagawa, Mitsujiro Osawa, Yoshimi Yashiro, Shinya Yamanaka, Kenji Osafune
PHYSIOLOGICAL REVIEWS
(2019)
Article
Cell & Tissue Engineering
Huaigeng Xu, Bo Wang, Miyuki Ono, Akihiro Kagita, Kaho Fujii, Noriko Sasakawa, Tatsuki Ueda, Peter Gee, Misato Nishikawa, Masaki Nomura, Fumiyo Kitaoka, Tomoko Takahashi, Keisuke Okita, Yoshinori Yoshida, Shin Kaneko, Akitsu Hotta
Article
Cell & Tissue Engineering
Masaki Yagi, Mio Kabata, Tomoyo Ukai, Sho Ohta, Akito Tanaka, Yui Shimada, Michihiko Sugimoto, Kimi Araki, Keisuke Okita, Knut Woltjen, Konrad Hochedlinger, Takuya Yamamoto, Yasuhiro Yamada
Article
Cell & Tissue Engineering
Yutaka Arai, Miho Takami, Yuri An, Mami Matsuo-Takasaki, Yasuko Hemmi, Tamami Wakabayashi, Jun Inoue, Michiya Noguchi, Yukio Nakamura, Keisuke Sugimoto, Tsukasa Takemura, Keisuke Okita, Kenji Osafune, Minoru Takasato, Tadayoshi Hayata, Yohei Hayashi
STEM CELL RESEARCH
(2020)
Article
Neurosciences
Yoshifumi Miyawaki, Bumpei Samata, Tetsuhiro Kikuchi, Kaneyasu Nishimura, Jun Takahashi
JOURNAL OF NEUROSCIENCE RESEARCH
(2020)
Article
Multidisciplinary Sciences
Daisuke Doi, Hiroaki Magotani, Tetsuhiro Kikuchi, Megumi Ikeda, Satoe Hiramatsu, Kenji Yoshida, Naoki Amano, Masaki Nomura, Masafumi Umekage, Asuka Morizane, Jun Takahashi
NATURE COMMUNICATIONS
(2020)
Article
Cell & Tissue Engineering
Takahiro Kitahara, Hideya Sakaguchi, Asuka Morizane, Tetsuhiro Kikuchi, Susumu Miyamoto, Jun Takahashi
Article
Biochemistry & Molecular Biology
Tatsuya Shimizu, Shin-Ichi Mae, Toshikazu Araoka, Keisuke Okita, Akitsu Hotta, Kunihiro Yamagata, Kenji Osafune
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Xinyue Yuan, Manuel Schroter, Marie Engelene J. Obien, Michele Fiscella, Wei Gong, Tetsuhiro Kikuchi, Aoi Odawara, Shuhei Noji, Ikuro Suzuki, Jun Takahashi, Andreas Hierlemann, Urs Frey
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Takayuki Hamabata, Katsutsugu Umeda, Kagehiro Kouzuki, Takayuki Tanaka, Tomoo Daifu, Seishiro Nodomi, Satoshi Saida, Itaru Kato, Shiro Baba, Hidefumi Hiramatsu, Mitsujiro Osawa, Akira Niwa, Megumu K. Saito, Yasuhiko Kamikubo, Souichi Adachi, Yoshiko Hashii, Akira Shimada, Hiroyoshi Watanabe, Kenji Osafune, Keisuke Okita, Tatsutoshi Nakahata, Kenichiro Watanabe, Junko Takita, Toshio Heike
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Yuta Yamamura, Kengo Furuichi, Yasuhiro Murakawa, Shigeki Hirabayashi, Masahito Yoshihara, Keisuke Sako, Shinji Kitajima, Tadashi Toyama, Yasunori Iwata, Norihiko Sakai, Kazuyoshi Hosomichi, Philip M. Murphy, Atsushi Tajima, Keisuke Okita, Kenji Osafune, Shuichi Kaneko, Takashi Wada
Summary: This study analyzed gene expression in iPSCs from patients with RCS carrying PAX2 mutations, identifying differentially activated candidate promoters and enhancers, and prioritized 17 candidate genes, with PBX1, POSTN, and ITGA9 ranked as the top three. The identification of these genes may provide important information for clarifying the pathogenesis of RCS, human kidney development, and kidney regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Shihori Yokobayashi, Yukihiro Yabuta, Masato Nakagawa, Keisuke Okita, Bo Hu, Yusuke Murase, Tomonori Nakamura, Guillaume Bourque, Jacek Majewski, Takuya Yamamoto, Mitinori Saitou
Summary: The research demonstrates significant epigenomic heterogeneity in hiPSCs, with different germline potential correlating with X chromosome inactivation. This heterogeneity is primarily determined by genomic properties.
Article
Cell & Tissue Engineering
Yoshinori Endo, Ken-ichiro Kamei, Kouichi Hasegawa, Keisuke Okita, Hideyuki Ito, Shiho Terada, Miho Inoue-Murayama
Summary: This study generated iPSCs from the endangered Grevy's zebra and described their molecular characteristics. The results suggest evolutionary conservation between these iPSCs and those from humans and mice, to some extent.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Cell & Tissue Engineering
Takafumi Shimogawa, Hideya Sakaguchi, Tetsuhiro Kikuchi, Ryosuke Tsuchimochi, Noritaka Sano, Sadaharu Torikoshi, Akira Ito, Tomoki Aoyama, Koji Iihara, Jun Takahashi
NPJ REGENERATIVE MEDICINE
(2019)
Article
Biochemical Research Methods
Aline Silva da Cruz, Maria Margarida Drehmer, Wagner Baetas-da-Cruz, Joao Carlos Machado
Summary: This study quantified microcirculation cerebral blood flow in a rat model of ischemic stroke using ultrasound biomicroscopy and ultrasound contrast agents. The results showed high sensitivity and specificity of this method, making it a valuable tool for preclinical studies.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Christina Dalla, Ivana Jaric, Pavlina Pavlidi, Georgia E. Hodes, Nikolaos Kokras, Anton Bespalov, Martien J. Kas, Thomas Steckler, Mohamed Kabbaj, Hanno Wuerbel, Jordan Marrocco, Jessica Tollkuhn, Rebecca Shansky, Debra Bangasser, Jill B. Becker, Margaret McCarthy, Chantelle Ferland-Beckham
Summary: Many funding agencies have emphasized the importance of considering sex as a biological variable in experimental design to improve the reproducibility and translational relevance of preclinical research. Omitting the female sex from experimental designs in neuroscience and pharmacology can result in biased or limited understanding of disease mechanisms. This article provides methodological considerations for incorporating sex as a biological variable in in vitro and in vivo experiments, including the influence of age and hormone levels, and proposes strategies to enhance methodological rigor and translational relevance in preclinical research.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Wenyu Gu, Dongxu Li, Jia-Hong Gao
Summary: We developed a precise and rapid method for positioning and labelling triaxial OPMs on a wearable magnetoencephalography (MEG) system, improving the efficiency of OPM positioning and labelling.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Kai Lin, Linhang Zhang, Jing Cai, Jiaqi Sun, Wenjie Cui, Guangda Liu
Summary: The article introduces an EEG feature map processing model for emotion recognition, which achieves significantly improved accuracy by fusing EEG information at different spatial scales and introducing a channel attention mechanism.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
John E. Parker, Asier Aristieta, Aryn H. Gittis, Jonathan E. Rubin
Summary: This work presents a toolbox that implements a methodology for automated classification of neural responses based on spike train recordings. The toolbox provides a user-friendly and efficient approach to detect various types of neuronal responses that may not be identified by traditional methods.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Yun Liang, Ke Bo, Sreenivasan Meyyappan, Mingzhou Ding
Summary: This study compared the performance of SVM and CNN on the same datasets and found that CNN achieved consistently higher classification accuracies. The classification accuracies of SVM and CNN were generally not correlated, and the heatmaps derived from them did not overlap significantly.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Antonino Visalli, Maria Montefinese, Giada Viviani, Livio Finos, Antonino Vallesi, Ettore Ambrosini
Summary: This study introduces an analytical strategy that allows the use of mixed-effects models (LMM) in mass univariate analyses of EEG data. The proposed method overcomes the computational costs and shows excellent performance properties, making it increasingly important in the field of neuroscience.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Xavier Cano-Ferrer, Alexandra Tran -Van -Minh, Ede Rancz
Summary: This study developed a novel rotation platform for studying neural processes and spatial navigation. The platform is modular, affordable, and easy to build, and can be driven by the experimenter or animal movement. The research demonstrated the utility of the platform, which combines the benefits of head fixation and intact vestibular activity.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
