Article
Food Science & Technology
Lin Wang, Liu Liu, Xiaotong Zhou
Summary: This study discovered that silver nanomaterial AgDAMP has antibacterial effects on Salmonella by damaging the cell membrane, inhibiting biofilm formation, and altering protein expression. It also found that AgDAMP significantly affects regulators associated with cell functions, such as DNA replication, protein synthesis, membrane transport, cell motility, and oxidative stress. These findings open up new possibilities for the treatment of foodborne pathogens using AgDAMP.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Immunology
Laia Fernandez-Barat, Nil Vazquez Burgos, Victoria Alcaraz, Leticia Bueno-Freire, Ruben Lopez-Aladid, Roberto Cabrera, Albert Gabarrus, Andrea Palomeque, Patricia Oscanoa, Adrian Ceccato, Ana Motos, Rosanel Amaro, Thierry Bernardi, Christian Provot, Alba Soler-Comas, Laura Munoz, Jordi Vila, Antoni Torres
Summary: The aim of this study was to validate and identify additional applications of the BioFilm Ring Test (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE), and evaluate its relationship with antimicrobial stewardship. The results showed that a BPI value below 14.75 at 5 hours predicted the mucoid PA phenotype. In conclusion, the BRT has the potential to reveal biofilm features with clinical implications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Mark C. Butcher, Jason L. Brown, Donald Hansom, Rebecca Wilson-Van Os, Craig Delury, Phillip A. Laycock, Gordon Ramage
Summary: Candida auris poses a substantial global nosocomial threat and can colonize the skin, particularly in surgical patients. This study found that antifungal-loaded calcium sulfate beads have the potential to interfere with the growth of C.auris.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Engineering, Biomedical
Leman Buzoglu Kurnaz, Swagatam Barman, Xiaoming Yang, Claire Fisher, F. Wayne Outten, Prakash Nagarkatti, Mitzi Nagarkatti, Chuanbing Tang
Summary: Inspired by the facial amphiphilic nature and antimicrobial efficacy of antimicrobial peptides, this study developed a new class of facial amphiphilic bicyclic naphthoic acid derivatives that were attached to poly(glycidyl methacrylate). These polymers exhibited potent antibacterial activity against multi-drug resistant gram-negative pathogens and showed low hemolysis and high antimicrobial selectivity. They were also effective in eradicating biofilms and killing dormant cells, suggesting a mechanism of action through membrane disruption. Overall, these facial amphiphiles have the potential to address antimicrobial resistance in both planktonic and biofilm-related infections.
Article
Microbiology
Mark C. Butcher, Jason L. Brown, Donald Hansom, Rebecca Wilson-van Os, Craig Delury, Phillip A. Laycock, Gordon Ramage
Summary: The study showed that antifungal-loaded CS beads effectively inhibit pathogenic fungi growth within 7 days, reducing biofilm formation and providing sustained antimicrobial effect against clinically relevant fungal species.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Environmental Sciences
Samuel Namonyo, Karen D. Weynberg, Jianhua Guo, Gilda Carvalho
Summary: This study investigated the role of phages in disrupting and inactivating clinical P. aeruginosa biofilms. Four previously isolated phages were effective in disrupting the formed biofilms at a multiplicity of infection (MOI) of 10. Phage treatments reduced the biofilms' biomass and led to the detachment of cells. The phages were also able to kill the cells from the biofilms, reducing the living cell counts.
ENVIRONMENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Jianye Zeng, Dandan Chen, Chunli Lv, Kening Qin, Qin Zhou, Na Pu, Shanshan Song, Xiaomin Wang
Summary: The aqueous extract of Polygonum chinense L. has a significant bacteriostatic effect on Staphylococcus aureus and can destroy its cell wall as well as inhibit biofilm formation. Flow cytometry results show that the extract can disrupt the cell membrane and promote apoptosis. Confocal laser scanning microscopy images demonstrate the dose-dependent inhibition of biofilm formation by the extract.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Farheen Akhtar, Asad U. Khan
Summary: Biofilm mediated infections, particularly those caused by Staphylococcus aureus, have a significant clinical impact. The emergence of multidrug-resistant strains makes treatment more challenging, necessitating the development of new strategies to combat such infections. In this study, curcumin-mediated antimicrobial photodynamic therapy showed promise in disrupting preformed VRSA biofilms and enhancing phagocytosis, providing a potential alternative treatment approach.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2021)
Article
Chemistry, Applied
Maria Aparecida Alexandre Josino, Cecilia Rocha da Silva, Jose Batista de Andrade Neto, Fatima Daiana Dias Barroso, Lisandra Juvencio da Silva, Bruno Coelho Cavalcanti, Manoel Odorico de Moraes, Debora Hellen Almeida Brito, Nagila Maria Pontes Silva Ricardo, Helio Vitoriano Nobre Junior
Summary: The study aims to evaluate the efficacy of fluoxetine microparticles against strains of Staphylococcus aureus sensitive and resistant to methicillin. The research showed that fluoxetine encapsulated in microparticles has the potential to act as an effective antimicrobial agent.
CARBOHYDRATE POLYMERS
(2021)
Article
Environmental Sciences
Bing Li, Yongjie Zhang, Qi Guo, Siyuan He, Junsheng Fan, Liyun Xu, Zhemin Zhang, Wenye Wu, Haiqing Chu
Summary: The study investigated the anti-biofilm activity of the antibacterial peptide RP557 against Mycobacterium abscessus, demonstrating that RP557 significantly increased the antibiotic sensitivity of the bacteria in biofilms. Scanning electron microscope and fluorescence staining were used to visualize the inhibitory effects of RP557 on biofilm formation and increased bacterial death.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Aditya Upadhyay, Dharm Pal, Awanish Kumar
Summary: Biofilm is a closed bacterial structure that provides survival capacity to bacterial community and creates resistance against antibiotics. Conventional therapies are not effective in controlling biofilm, making the need for a novel approach urgent. Combinatorial enzyme therapy shows promise in disrupting biofilm and combating multi-drug resistance effectively. This review discusses the potential and future perspective of combinatorial enzyme therapies in disrupting bacterial biofilm.
PROCESS BIOCHEMISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
John S. Colombo, Hammad Malik, Christian Ariana Caranto, Patrina K. Allen, Jerry Howard, Krista Carlson
Summary: This technology efficiently produces oxidants that eliminate bacteria without harming native pulp cells after relatively brief exposures. Incomplete disinfection of inflamed dental pulp is a significant cause of pulp destruction, leading to devitalization or extraction.
JOURNAL OF DENTISTRY
(2022)
Article
Biochemistry & Molecular Biology
Min Ji Hong, Min Kyung Kim, Yoonkyung Park
Summary: The analog peptides derived from Hp1404 exhibit antimicrobial activity against multidrug-resistant Acinetobacter baumannii with lower cytotoxicity. They effectively permeate bacterial membranes and inhibit biofilm formation, showing potential as therapeutic agents for A. baumannii infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Haiyan Gong, Lijuan He, Zhilong Zhao, Xinmin Mao, Chen Zhang
Summary: The study indicates that PU has significant antibacterial and antibiofilm formation activity against E. coli K1, inhibiting biofilm formation and destroying mature biofilm. The research also identified pgaA as a potential target of PU in E. coli.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Immunology
Dibyajit Lahiri, Moupriya Nag, Ritwik Banerjee, Dipro Mukherjee, Sayantani Garai, Tanmay Sarkar, Ankita Dey, Hassan I. Sheikh, Sushil Kumar Pathak, Hisham Atan Edinur, Siddhartha Pati, Rina Rani Ray
Summary: Biofilm is a symbiotic association of microbial cells that adhere to surfaces with the help of pili and EPS, which also prevent penetration of antimicrobials. Enzymes, particularly amylase, play important roles in removing biofilms by inhibiting EPS formation. However, salivary amylase can initiate biofilm formation by binding to specific proteins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Michael Paterson, William Johnston, Andrea Sherriff, Shauna Culshaw
Summary: This post hoc analysis aimed to compare the clinical outcomes of non-surgical treatment for periodontal disease using hand instruments, ultrasonic instruments, or a combination approach. The study found that ultrasonic instruments had shorter treatment time on average and were more cost-effective.
BRITISH DENTAL JOURNAL
(2023)
Article
Immunology
J. Malcolm, M. H. Nyirenda, J. L. Brown, A. Adrados-Planell, L. Campbell, J. P. Butcher, D. G. Glass, K. Piela, C. S. Goodyear, A. J. Wright, I. B. McInnes, O. R. Millington, S. Culshaw
Summary: The emergence of anti-citrullinated autoimmunity in rheumatoid arthritis is associated with specific complexes. It has been discovered that C-terminal citrullination by Porphyromonas gingivalis can generate autoantigens and breach T cell tolerance. This study identifies the mechanisms and pathways involved in the breach of tolerance and provides new insights into the development of anti-citrullinated autoimmunity in rheumatoid arthritis.
JOURNAL OF AUTOIMMUNITY
(2023)
Article
Cell & Tissue Engineering
Saeed Alqahtani, Mark C. C. Butcher, Gordon Ramage, Matthew J. J. Dalby, William McLean, Christopher J. J. Nile
Summary: Mesenchymal stem cells (MSCs) have the potential to regenerate tissues. However, the signaling mechanisms and pathways that control their proliferation and differentiation are not fully understood. Recent studies suggest that the non-neuronal cholinergic signaling system, specifically acetylcholine (ACh), plays a significant role in cell-to-cell communication and stem cell function. ACh receptors (AChRs) have been identified in MSCs and have been found to impact their regenerative potential. This review summarizes the expression and function of AChRs in different types of MSCs, highlighting their pivotal role in regulating MSC regenerative function.
STEM CELLS AND DEVELOPMENT
(2023)
Article
Microbiology
Bryn Short, Ahmed Bakri, Abdullah Baz, Craig Williams, Jason Brown, Gordon Ramage
Summary: The management of chronic wounds is a challenging task and a burden on healthcare professionals and services. Chronic wounds are susceptible to microbial infection, which worsens their management and clinical outcomes. However, the role of fungi in chronic wounds is largely ignored, and their influence is not fully understood.
CURRENT CLINICAL MICROBIOLOGY REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Nishtha Gaur, Bethany L. Patenall, Bhagirath Ghimire, Naing T. Thet, Jordan E. Gardiner, Krystal E. Le Doare, Gordon Ramage, Bryn Short, Rachel A. Heylen, Craig Williams, Robert D. Short, Toby A. Jenkins
Summary: We introduce a versatile drug-loaded composite hydrogel that can simultaneously deliver a drug and CAP-generated molecules in a tissue target when activated by an argon-based cold atmospheric plasma jet. We demonstrated this concept by encapsulating gentamicin in sodium polyacrylate particles dispersed within a poly(vinyl alcohol) hydrogel matrix. The resulting gentamicin-PAA-PVA composite hydrogel can release gentamicin and effectively eradicate bacteria, including those in planktonic state and biofilm, when activated by CAP. The concept of the composite hydrogel can also be applied to other antimicrobial agents like cetrimide and silver, and activated by any dielectric barrier discharge CAP device.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Immunology
Gordon Ramage, Elisa Borghi, Celia Fortuna Rodrigues, Ryan Kean, Craig Williams, Jose Lopez-Ribot
Summary: Clinically we have long been aware of the concept of Candida biofilms, which have the ability to colonize surfaces and form tenacious structures. These infections, found in various body sites, are highly resistant to antifungal therapies. This review provides a comprehensive overview of current clinical understanding, as well as existing and emerging antifungal therapies and strategies.
Article
Immunology
Christopher Delaney, Chandra Lekha Ramalingam Veena, Mark C. Butcher, William McLean, Suror Mohamad Ahmad Shaban, Christopher J. Nile, Gordon Ramage
Summary: A new era of next-generation sequencing has revealed the role of the oral microbiome in oral squamous cell carcinoma (OSCC). This study conducted a meta-analysis of 58 studies to identify trends and differences in the 16S rRNA oral microbiome between OSCC cases and healthy controls. The results showed no significant differences in diversity between health and OSCC, but certain species were found to be indicative of disease. Further standardization of study design and methodology is needed to identify biomarkers for screening and diagnostics.
Article
Dentistry, Oral Surgery & Medicine
William Johnston, Bob T. Rosier, Miguel Carda-Dieguez, Michael Paterson, Paddy Watson, Krystyna Piela, Marilyn Goulding, Gordon Ramage, Divyashri Baranyia, Tsute Chen, Nezar N. Al-Hebshi, Alex Mira, Shauna Culshaw
Summary: This study investigated the early and later changes in subgingival biofilm following periodontal treatment and found that hand and ultrasonic instruments had similar impacts on the composition of the subgingival plaque microbiome. There were marked changes in biofilm composition in the early stages after treatment, but limited evidence of these changes being associated with treatment outcomes.
JOURNAL OF PERIODONTOLOGY
(2023)
Editorial Material
Immunology
Gordon Ramage, Ase Bengard Andersen
Review
Biochemistry & Molecular Biology
Susanth Alapati, Giulio Fortuna, Gordon Ramage, Christopher Delaney
Summary: This systematic review explores the application of metabolomics in identifying biomarkers and metabolic pathways for oral squamous cell carcinoma (OSCC) across multiple studies. Thirteen longitudinal studies involving OSCC patients and metabolic marker analysis were included. The dysregulated metabolites in OSCC primarily involve amino acids and are associated with enriched pathways such as aminoacyl-tRNA biosynthesis, glutathione metabolism, and arginine biosynthesis. This review highlights the potential of metabolomics for early diagnosis and therapeutic targeting of OSCC.
Meeting Abstract
Gastroenterology & Hepatology
K. Gerasimidis, B. Nichols, R. Papadopoulou, U. Ijaz, R. Rajendran, C. Quince, R. K. Russell, R. Hansen, G. Ramage
JOURNAL OF CROHNS & COLITIS
(2023)
Article
Microbiology
Olabayo H. Ajetunmobi, Hamid Badali, Jesus A. Romo, Gordon Ramage, Jose L. Lopez-Ribot
Summary: Virtually all Candida species linked to clinical candidiasis are capable of forming highly resistant biofilms on different types of surfaces, which poses an additional significant threat and further complicates therapy of these infections. The scarcity and limited effectiveness of antifungal agents, particularly against biofilms, are major challenges in the treatment of Candida biofilms. However, there is optimism that these challenges can be overcome in a reasonable timeframe.
Article
Microbiology
Abdullah Baz, Ahmed Bakri, Mark Butcher, Bryn Short, Bhagirath Ghimire, Nishtha Gaur, Toby Jenkins, Robert D. Short, Marcello Riggio, Craig Williams, Gordon Ramage, Jason L. Brown
Summary: The clinical and socioeconomic impact of chronic wounds is significant. The main challenge in treating chronic wounds is the risk of infection, particularly from biofilms that are resistant to antibiotics. Cold atmospheric plasma (CAP) has shown promising antimicrobial and immunomodulatory properties and can effectively treat biofilm infections. This study evaluated the efficacy of CAP in treating different biofilm models and found that it effectively killed Candida albicans, Pseudomonas aeruginosa, and Candida auris, but Staphylococcus aureus showed some degree of tolerance.
Article
Microbiology
Christopher Delaney, Bryn Short, Ranjith Rajendran, Ryan Kean, Karl Burgess, Craig Williams, Carol A. Munro, Gordon Ramage
Summary: Candida albicans is a prevalent and notorious fungus involved in bloodstream infections with the ability to form robust biofilms. The mechanisms underlying adaptive biofilm heterogeneity have not yet been fully explored. In this study, we assessed the susceptibility of different populations of C. albicans isolates to antifungal treatments and studied the transcriptional and metabolic reprogramming in response to biofilm formation.
Article
Microbiology
William Johnston, Alicia Ware, Willemijn Frederique Kuiters, Christopher Delaney, Jason Lee Brown, Suzanne Hagen, David Corcoran, Matthew Cummings, Gordon Ramage, Ryan Kean
Summary: Bacterial vaginosis (BV) affects a significant percentage of women globally and increases the risk of miscarriage and pre-term birth. Antibiotics are usually ineffective in treating BV due to the formation of resistant biofilms. In this study, a four-species biofilm was developed to mimic BV infection, and a Gardnerella-specific endolysin showed efficacy against G. vaginalis within the biofilms. This study highlights the resilience of biofilm-embedded pathogens and provides a model for more effective screening of BV therapies.