Article
Microbiology
Gaku Harata, Kazutoyo Yoda, Ruipeng Wang, Kenji Miyazawa, Masayuki Sato, Fang He, Akihito Endo
Summary: This study found age/generation-related differences in the adhesion properties of B. bifidum and other strains, with B. bifidum showing stronger adhesion to neonatal intestinal mucus, suggesting a deeper symbiotic relationship with infants, particularly neonates.
Article
Biotechnology & Applied Microbiology
Veera Kainulainen, Carina Von Schantz-Fant, Ruusu-Maria Kovanen, Swapnil Potdar, Karoliina Laamanen, Jani Saarela, Reetta Satokari
Summary: In this study, a high-throughput screening assay was developed to explore the binding receptors of intestinal epithelial cells for Bifidobacterium bifidum. Four cell surface proteins, SERPINB3, LGICZ1, PKD1, and PAQR6, were identified as potential receptors. Blocking these host cell proteins significantly decreased the binding of B. bifidum to the intestinal cell lines. These proteins are involved in the regulation of cellular processes related to proliferation, differentiation, apoptosis, inflammation, and immunity, providing insights into the mechanisms of B. bifidum-host interactions and its regulation of intestinal homeostasis.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Biotechnology & Applied Microbiology
Nayoun Hong, Seockmo Ku, Kyungjin Yuk, Tony V. Johnston, Geun Eog Ji, Myeong Soo Park
Summary: Recombinant B. bifidum BGN4 [pBESIL10] efficiently produces and secretes significant amounts of biologically active human IL-10, with the highest production level reported to date. Further research should explore the potential of B. bifidum BGN4 [pBESIL10] producing IL-10 as a treatment for various inflammation-related diseases, including inflammatory bowel disease, rheumatoid arthritis, allergic asthma, and cancer immunotherapy.
MICROBIAL CELL FACTORIES
(2021)
Article
Nutrition & Dietetics
Nan Tang, Qiangqing Yu, Chunxia Mei, Jialiang Wang, Linlin Wang, Gang Wang, Jianxin Zhao, Wei Chen
Summary: This study found that Bifidobacterium bifidum CCFM1163 can effectively alleviate cathartic colon symptoms by modulating gut microbiota and intestinal barrier function, improving intestinal motility, and relieving constipation.
Article
Food Science & Technology
Yingxue Yue, Yuqi Wang, Yu Han, Yifan Zhang, Ting Cao, Guicheng Huo, Bailiang Li
Summary: The study evaluated the antioxidant properties of intact cells, cell-free supernatant, and cell-free extracts, as well as the genome sequencing and structural characteristics of Bifidobacterium bifidum E3. The results showed that the cell-free supernatant had the highest scavenging capacities for different radicals. The EPS produced by B. bifidum E3 exhibited strong antioxidant properties and may have health-promoting benefits in humans.
Article
Nutrition & Dietetics
Rui Wang, Malina Kuerman, Qingyu Cui, Xiaoying Tian, Yu Zhou, Huaxi Yi, Pimin Gong, Kai Lin, Zhe Zhang, Tongjie Liu, Lanwei Zhang
Summary: This study investigates the potential mechanisms of different probiotic strains in preventing intestinal barrier damage. The results show that Bifidobacterium bifidum FL-228.1 exhibits the best prophylactic effects in protecting against intestinal barrier damage. Further research suggests that FL-228.1 achieves this by enhancing the production of Muc2 and Cldn-4, inhibiting NLRP3 activation, and activating PPAR gamma and TLR2.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Ahmed G. Abdelhamid, Noha K. El-Dougdoug
Summary: Bifidobacterium bifidum is genetically similar and functionally versatile in utilizing carbohydrates present in the host's system, as evidenced by the presence of diverse Carbohydrate-Active Enzyme (CAZY) systems across strains from different body niches. Comparative genomics highlighted the shared orthologous gene families among B. bifidum strains, reinforcing their genetic similarity and potential functional consistency in carbohydrate utilization.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Agriculture, Multidisciplinary
Yingxue Yue, Yuqi Wang, Qinggang Xie, Xiuli Lv, Linyi Zhou, Etareri Evivie Smith, Ting Cao, Yifan Zhang, Bailiang Li, Guicheng Huo, Weiwei Ma
Summary: Changes in intestinal barrier functions during sepsis were evaluated by injecting lipopolysaccharide (LPS) into mice. The probiotic effect of Bifidobacterium strains (BB, BL, BB12, and BLBB) was studied. The BLBB group showed improved ileal villus length, increased immune cells, reduced serum levels of DAO and D-lactic acid, increased expression of tight junction proteins and mucin, decreased inflammatory cytokines, and altered gut microbiota composition. Bifidobacterium bifidum E3 combined with Bifidobacterium longum subsp. infantis E4 showed promise in protecting against LPS-induced intestinal injury.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Yu Tang, Chun Chen, Binglei Jiang, Lu Wang, Fujie Jiang, Disen Wang, Yaotai Wang, Haiyan Yang, Xia Ou, Yan Du, Qi Wang, Jianzhong Zou
Summary: The novel therapy method of BF-mediated HIFU ablation effectively targets tumors using Bifidobacterium bifidum, significantly enhancing therapeutic efficiency and prolonging survival time in mice without obvious side effects, offering a promising platform for non-invasive tumor treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Food Science & Technology
Dalia G. Kamel, Ahmed R. A. Hammam, Khalid A. Alsaleem, Dina M. Osman
Summary: The study found that the addition of inulin and storage time significantly affected the titratable acidity of probiotic yogurt, with inulin enhancing the viability of Bifidobacterium bifidum and acting as an antimicrobial against molds.
FOOD SCIENCE & NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Linlin Wang, Ting Jiao, Qiangqing Yu, Jialiang Wang, Luyao Wang, Gang Wang, Hao Zhang, Jianxin Zhao, Wei Chen
Summary: The occurrence of non-alcoholic fatty liver disease (NAFLD) is closely related to intestinal microbiota disturbance. This study found that Bifidobacterium exerts species-specific effects on NAFLD, alleviating the disease by regulating the relative abundance of intestinal microbiota, increasing the levels of propionic acid and butyric acid, regulating lipid metabolism and intestinal permeability.
Article
Genetics & Heredity
Wenwei Lu, Zhangming Pei, Mengning Zang, Yuan-kun Lee, Jianxin Zhao, Wei Chen, Hongchao Wang, Hao Zhang
Summary: The research found that Bifidobacterium bifidum has an open pan-genome and a conserved core genome, with genetic diversity mainly reflected in carbohydrate metabolism and immune/competition-related factors. In addition, a novel CRISPR-Cas system was discovered in B. bifidum through this study.
Article
Biotechnology & Applied Microbiology
Keita Nishiyama, Cheng-Chung Yong, Nobuko Moritoki, Haruki Kitazawa, Toshitaka Odamaki, Jin-Zhong Xiao, Takao Mukai
Summary: The study reveals a new symbiotic relationship between two bacterial strains, B. longum and B. thetaiotaomicron, mediated by the sharing of moonlighting proteins. These proteins affect the adhesion of bacteria to mucins, and they were found to be present on the surface of B. thetaiotaomicron. Moreover, the recombinant proteins were observed to bind to the cell surface of several other bacterial species, albeit with species-dependent binding.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Food Science & Technology
Raquel Marcos-Fernandez, Aitor Blanco-Miguez, Lorena Ruiz, Abelardo Margolles, Patricia Ruas-Madiedo, Borja Sanchez
Summary: This work describes a new strain of Bifidobacterium bifidum, IPLA60003, which exhibits aerotolerance and the ability to form colonies under aerobic conditions, a phenotype never observed before in this species. The strain IPLA60003 was obtained through random UV mutagenesis and carries 26 single nucleotide polymorphisms that activate oxidative-defense mechanisms. The study discusses the molecular mechanisms underlying the aerotolerance phenotype and its implications for the selection and inclusion of probiotic strains in functional foods.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Medicinal
Hyeong Sim Choi, Young-Kyun Kim, Pil-Young Yun
Summary: This study aimed to identify the gene expressions related to cisplatin resistance in oral squamous cell carcinoma (OSCC) cell lines. RNA sequencing (RNA-Seq) analysis was used to determine differentially expressed genes and perform functional enrichment and signaling pathway analyses. Several genes related to cell adhesion molecules and proteoglycans in cancer pathways were found to have altered expression in cisplatin-resistant samples. These findings may provide new insights for improving the clinical therapeutic outcomes of OSCC.
Article
Biochemistry & Molecular Biology
Valentino Le Noci, Giancarla Bernardo, Giacomo Manenti, Gabriele Infante, Dariush Khaleghi Hashemian, Lucia Minoli, Simone Canesi, Francesca Bianchi, Tiziana Triulzi, Stefania Arioli, Loris De Cecco, Simone Guglielmetti, Federico Ambrogi, Camilla Recordati, Nicoletta Gagliano, Elda Tagliabue, Michele Sommariva, Lucia Sfondrini
Summary: This study explores the impact of perturbing lung microbiota on lung cancer development by using antibiotics or probiotic aerosol. The results suggest that L.RGG aerosol can reduce tumor nodules and Tregs in the lung, while increasing the expression of immunoglobulin joining chain and promoting B and CD4 T cell infiltration. This non-invasive strategy shows efficacy in impairing lung cancer growth by promoting local immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Gastroenterology & Hepatology
Giacomo Mantegazza, Giorgio Gargari, Simone Guglielmetti
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Oncology
Giancarla Bernardo, Valentino Le Noci, Emerenziana Ottaviano, Loris De Cecco, Chiara Camisaschi, Simone Guglielmetti, Martina Di Modica, Giorgio Gargari, Francesca Bianchi, Serena Indino, Patrizia Sartori, Elisa Borghi, Michele Sommariva, Elda Tagliabue, Tiziana Triulzi, Lucia Sfondrini
Summary: This study highlights the significant influence of mammary tumor microbiota on local immune status and the relevance of its treatment with antibiotics, in combination with breast cancer therapies.
Article
Food Science & Technology
Giacomo Mantegazza, Alessandro Dalla Via, Armando Licata, Robin Duncan, Claudio Gardana, Giorgio Gargari, Cristina Alamprese, Stefania Arioli, Valentina Taverniti, Matti Karp, Simone Guglielmetti
Summary: This study selected safe food microorganisms and used them to ferment soy drinks, resulting in a final product with increased estrogenic activity and improved functional properties. The experiment showed that two strains of Leuconostoc in fermented soy milk increased the estrogenic activity of the drink. These findings meet the growing demand for health-promoting plant-based alternatives to dairy products.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Andela Martinovic, Marco Chittaro, Diego Mora, Stefania Arioli
Summary: This study evaluates the impact of manufacturing conditions on the in vivo functionality of Streptococcus thermophilus BT01 as a probiotic, as well as its ability to modulate urease activity in fecal samples. Results show that the type of cryoprotectant used in the manufacturing process does not significantly affect the viability of S. thermophilus BT01 in fecal samples, but does impact its ability to reduce urease activity. This study highlights the importance of manufacturing conditions in probiotic functionality and provides the first evidence linking S. thermophilus to a specific probiotic mechanism.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Microbiology
Giacomo Mantegazza, Giorgio Gargari, Robin Duncan, Fabio Consalez, Valentina Taverniti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigated the presence of bacteria in commercial ready-to-eat rocket salads and found that these salads could be a source of lactic acid bacteria and other microbes that are capable of surviving the digestive system, potentially increasing the diversity of the gut microbiota. This finding may apply to raw vegetables in general.
MICROBIOLOGY SPECTRUM
(2023)
Article
Nutrition & Dietetics
Michela Carola Speciani, Giorgio Gargari, Roberto Penagini, Massimiliano Mutignani, Monica Ferraroni, Arianna Natale, Michail Katsoulis, Marcello Cintolo, Pierfrancesco Leone, Aldo Airoldi, Maurizio Vecchi, Rossella Bonzi, Clorinda Ciafardini, Barbara Oreggia, Pietro Carnevali, Simone Guglielmetti, Patrizia Riso, Carlo La Vecchia, Marta Rossi
Summary: Garlic consumption is inversely associated with the risk of intestinal adenoma and colorectal cancer. This study found that medium/high garlic consumption is related to a decrease in the risk of colorectal cancer and is associated with changes in specific blood bacterial taxa.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Food Science & Technology
Meriem Meradji, Nadia Bachtarzi, Diego Mora, Karima Kharroub
Summary: Using culture enrichment methods, 100 strains of lactic acid bacteria (LAB) were isolated from honeybee Apis mellifera intermissa and fresh honey. Amongst the isolated LAB, 19 selected strains showed potential probiotic properties and safety characteristics. This study highlights the potential of honeybee and its products as a source of novel LAB with probiotic features.
Article
Health Care Sciences & Services
Paraskevi Massara, Arooj Asrar, Celine Bourdon, Moses Ngari, Charles D. G. Keown-Stoneman, Jonathon L. Maguire, Catherine S. Birken, James A. Berkley, Robert H. J. Bandsma, Elena M. Comelli
Summary: This study assessed the performance of six methods for detecting different types of outliers, proposed two novel methods for outlier trajectory detection, and evaluated the impact of outliers on growth pattern detection. The results showed that model-based outlier detection methods performed best for measurements, especially for low and moderate error intensities. The clustering-based outlier trajectory method performed exceptionally well across all types and intensities of errors. Comparing growth groups with and without outliers demonstrated that outliers can alter group membership.
BMC MEDICAL RESEARCH METHODOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Review
Food Science & Technology
Mirko Marino, Samuele Venturi, Giorgio Gargari, Cristian Del Bo, Daniela Martini, Marisa Porrini, Simone Guglielmetti, Patrizia Riso
Summary: Berries and their (poly)phenols play an important role in mediating the modulation of gut microbiota and human health. The evidence regarding the interaction between (poly)phenol intake and gut microbiota activity in humans is still unclear. This systematic review collects and assesses results from randomized clinical trials to explore the role of berries in the modulation of gut microbiota and their contribution to the metabolism of berry (poly)phenols.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Microbiology
Amel Taibi, Tomas Tokar, Julien Tremblay, Giorgio Gargari, Catherine J. Streutker, Bo Li, Agostino Pierro, Simone Guglielmetti, Thomas A. Tompkins, Igor Jurisica, Elena M. Comelli
Summary: The gut microbiota affects intestinal microRNA (miRNA) signatures and is modified by host-derived luminal miRNA. This suggests the existence of close miRNA-microbiota relationships that are critical to intestinal homeostasis. However, an integrative analysis of these relationships and their evolution during intestinal postnatal maturation is lacking.
Article
Food Science & Technology
Simone Guglielmetti
Summary: This review examines the safety concerns of using nonviable microbial cells in food and dietary supplements, drawing insights from probiotic research. It identifies four categories of risks and discusses the safety profile of nonviable microbes in human intervention trials. Furthermore, it highlights the potential benefits of using nonviable microorganisms as "next-generation probiotics."
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Gastroenterology & Hepatology
Giorgio Gargari, Giacomo Mantegazza, Valentina Taverniti, Claudio Gardana, Alice Valenza, Federico Rossignoli, Maria Raffaella Barbaro, Giovanni Marasco, Cesare Cremon, Giovanni Barbara, Simone Guglielmetti
Summary: This study analyzed fecal samples from IBS patients and healthy controls, and found significant differences in gut microbiota between the two groups, with higher intra-sample biodiversity in healthy controls. Furthermore, non-constipated patients could be classified into two subgroups based on their SCFA levels, each characterized by unique taxonomic bacterial signatures. The study provides a new perspective on the relationship between the gut microbiome and IBS, underscoring the importance of personalized strategies for management.
Article
Microbiology
Diana Wu, Lilian U. Thompson, Elena M. Comelli
Summary: The expression of miRNA in the mammary gland is related to the gut microbiota, and this relationship can be modified through dietary interventions to reduce the risk of breast cancer.
MICROBIOLOGY SPECTRUM
(2023)