Article
Biochemistry & Molecular Biology
Min Zhou, Qida Shen, Shasha Wang, Guoyong Li, Yan Wu, Caidi Xu, Bin Tang, Can Li
Summary: This study investigated the regulation of trehalose-6-phosphate synthase (TPS) in chitin metabolism in the brown planthopper. The results showed that inhibition of TPS expression affected the metabolism of trehalose and chitin in the insect. These findings provide a theoretical basis for pest control.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuhan Gao, Xiaoyu Yang, Xiong Yang, Tianyun Zhao, Xinmin An, Zhong Chen
Summary: This study identified 13 PtTPS and 10 PtTPP genes in the Populus genome, divided into two subfamilies with highly conserved intron structures, and involved in plant hormone and environmental stress responses. Expression patterns of PtTPSs and PtTPPs vary under different stress and treatment conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Physiology
Hong-Jia Yang, Meng-Yao Cui, Xiao-Hui Zhao, Chun-Yu Zhang, Yu-Shuo Hu, Dong Fan
Summary: In this study, the Trehalose-6-phosphate synthase (TPS) gene in Mythimna separata (MsTPS) was cloned and its expression patterns in different developmental stages and tissues were analyzed. The inhibition of MsTPS expression resulted in decreased trehalose content and changed expression of chitin synthase genes. It also had negative effects on M. separata larvae, including decreased weight, feeding capacity, and increased mortality and malformation rates.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Plant Sciences
Casey Gabriel, Jean-Jacque Fernhout, Franziska Fichtner, Regina Feil, John E. Lunn, Jens Kossmann, James R. Lloyd, Christell Van der Vyver
Summary: Manipulation of Tre6P/trehalose metabolism can alter the profile of soluble sugars accumulated in sugarcane stems. Overexpressing otsA and otsB genes or silencing native TPS expression in sugarcane led to changes in sucrose levels and the accumulation of other sugars in different tissues of the plant.
Article
Plant Sciences
Weipeng Wang, Hua Cui, Xiangfen Xiao, Bingjie Wu, Jialiang Sun, Yaxin Zhang, Qiuyue Yang, Yuping Zhao, Guoxiang Liu, Tengfei Qin
Summary: In this study, the TPP gene family in cotton was systematically identified and the function of GhTPP22 in drought stress conditions was preliminarily verified. These findings provide new information for improving drought resistance in cotton breeding in the future.
Article
Biochemistry & Molecular Biology
Chao Xu, Hongzhou Chen, Qinyan Wu, Yuqi Wu, Paul Daly, Jian Chen, Hongfu Yang, Lihui Wei, Yiqing Zhuang
Summary: This study found that NPP has a better inhibitory effect on F. graminearum, reducing DON production and affecting the trehalose biosynthesis pathway.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ye Tao, Yannick Rossez, Clovis Bortolus, Luminita Duma, Faustine Dubar, Franck Merlier
Summary: High-resolution mass spectrometry (HRMS) coupled with ultra-high-performance liquid chromatography (UHPLC) allows for simultaneous quantification of trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis enables metabolomic analyses and semi-quantification. The method has been validated for different matrices, yeasts, and bacteria, and can differentiate between bacteria based on growth temperatures.
Article
Entomology
Jia Wang, Huan Fan, Ying Li, Tong-Fang Zhang, Ying-Hong Liu
Summary: In this study, three TPP genes from Bactrocera minax were identified and characterized. The different functions of these genes in trehalose synthesis and metamorphosis were revealed. Suppression of these genes led to increased mortality and malformation rate in B. minax, suggesting their potential as insecticidal targets.
Article
Biochemistry & Molecular Biology
Mohanna Mollavali, Frederik Boernke
Summary: In plants, the trehalose biosynthetic pathway is important for regulating carbon allocation and stress adaptation, with engineering of this pathway showing promise for improving crop stress resilience. In tomato, the TPS/TPP family members show tissue-specific expression and respond to temperature shifts, indicating their potential role in temperature stress adaptation. The study suggests that the exploitation of TPS and TPP gene families could enhance tomato stress resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hagar Fox, Shifra Ben-Dor, Adi Doron-Faigenboim, Moshe Goldsmith, Tamir Klein, Rakefet David-Schwartz
Summary: By analyzing the genes related to NSC signaling, translocation, and metabolism in Populus alba under drought stress, this study reveals the molecular mechanisms regulating NSC management. The results show starch depletion across organs and accumulation of soluble sugars in the leaves. The activation of the SnRK1 signaling pathway and the upregulation of key genes support an active response to drought. Additionally, the study provides valuable information for tree improvement programs in adapting to drought conditions.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Wenjing Shao, Xinlin Zhang, Zhiheng Zhou, Yue Ma, Duo Chu, Lei Wang, Yiming Yang, Lin Du, Yanli Du, Jidao Du, Qiang Zhao
Summary: This study provides a comprehensive analysis of the TPP gene family in soybean and identifies key factors associated with abiotic stress response regulation. Furthermore, the study finds that exogenous trehalose treatment improves saline-alkali tolerance in soybean and reduces oxidative stress. These findings lay a foundation for further functional studies on the role of the TPP gene family in soybean breeding.
Article
Parasitology
ZhaoHai Wen, XinRan Xie, Muhammad Tahir Aleem, Kalibixiati Aimulajiang, Cheng Chen, Meng Liang, XiaoKai Song, LiXin Xu, XiangRui Li, RuoFeng Yan
Summary: The HcGOB gene is transcribed in different developmental stages of H. contortus, with the highest levels in the egg stage; rHcGOB is widely distributed in adult worms, mainly localized in the gut and gonads; rHcGOB binds to PBMCs and activates the IL-10/1 GF-beta/STAT3 pathway in vitro.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Rayne S. S. Magalhaes, Fernanda C. Boechat, Aline A. Brasil, Jose R. M. Neto, Gabriela D. Ribeiro, Luan H. Paranhos, Natalia Neves de Souza, Tuane Vieira, Tiago F. Outeiro, Bianca C. Neves, Elis C. A. Eleutherio
Summary: The research suggests that T6P may offer a new paradigm for cancer treatment based on specific inhibition of HK2, playing a crucial role in the survival and proliferation of cancer cells.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Gaopeng Yuan, Junpu Liu, Guolin An, Weihua Li, Wenjing Si, Dexi Sun, Yingchun Zhu
Summary: This study comprehensively analyzed the trehalose-6-phosphate synthase (TPS) gene family in watermelon and revealed its functional classification, evolutionary characteristics, expression patterns, and mechanisms related to salt stress. The study identified the critical role of ClTPS3 in watermelon response to salt stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Aleksandra Swida-Barteczka, Andrzej Pacak, Katarzyna Kruszka, Przemyslaw Nuc, Wojciech M. Karlowski, Artur Jarmolowski, Zofia Szweykowska-Kulinska
Summary: This study analyzed the expression of 150 conserved miRNAs in barley under drought stress and after rewatering, and identified target mRNAs for all but two miRNAs, with 41 newly reported targets. Drought stress induced accelerated flowering in barley through the regulation of two differently expressed miRNAs derived from the same precursor: hvu-miR172b-3p and hvu-miR172b-5p. The increase in miRNA172b-3p downregulated four APETALA2-like genes through mRNA cleavage, while the downregulation of miRNA172b-5p led to an increased level of trehalose-6-phosphate synthase, resulting in higher trehalose content in drought-treated plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Rayne S. S. Magalhaes, Karina C. De Lima, Diego S. G. de Almeida, Joelma F. De Mesquita, Elis C. A. Eleutherio
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Mariana Dias Castela de Carvalho, Joelma Freire De Mesquita, Elis Cristina Araujo Eleutherio
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Mauro B. Franca, Karina C. Lima, Elis C. A. Eleutherio
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Article
Biochemical Research Methods
Renata M. Santos, Fabio C. S. Nogueira, Aline A. Brasil, Paulo C. Carvalho, Felipe V. Leprevost, Gilberto B. Domont, Elis C. A. Eleutherio
JOURNAL OF PROTEOMICS
(2017)
Article
Neurosciences
Aline A. Brasil, Rayne S. S. Magalhes, Mariana D. C. De Carvalho, Isabel Paiva, Ellen Gerhardt, Marcos D. Pereira, Tiago F. Outeiro, Elis C. A. Eleutherio
MOLECULAR NEUROBIOLOGY
(2018)
Review
Mycology
Alene Alder-Rangel, Alexandre M. Bailao, Anderson F. da Cunha, Celia M. A. Soares, Chengshu Wang, Diego Bonatto, Ekaterina Dadachova, Elias Hakalehto, Elis C. A. Eleutherio, Everton K. K. Fernandes, Geoffrey M. Gadd, Gerhard H. Braus, Gilberto U. L. Braga, Gustavo H. Goldman, Iran Malavazi, John E. Hallsworth, Jon Y. Takemoto, Kevin K. Fuller, Laura Selbmann, Luis M. Corrochano, Marcia R. von Zeska Kress, Maria Celia Bertolini, Monika Schmoll, Nicolas Pedrini, Octavio Loera, Roger D. Finlay, Rosane M. Peralta, Drauzio E. N. Rangel
Article
Mycology
Jonas Paulino de Souza, Cleiton Dias do Prado, Elis C. A. Eleutherio, Diego Bonatto, Iran Malavazi, Anderson Ferreira da Cunha
Article
Mycology
Elis Eleutherio, Aline de Araujo Brasil, Mauro Braga Franca, Diego Seixas Gomes de Almeida, Germana Breves Rona, Rayne Stfhany Silva Magalhaes
Article
Biochemistry & Molecular Biology
Germana B. Rona, Natalia P. Almeida, Gilson C. Santos, Tatiana K. S. Fidalgo, Fabio C. L. Almeida, Elis C. A. Eleutherio, Anderson S. Pinheiro
JOURNAL OF CELLULAR BIOCHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Igor P. V. Vieira, Gabrielle T. Cordeiro, Diego E. B. Gomes, Rafael D. Melani, Leonardo F. Vilela, Gilberto B. Domont, Rafael D. Mesquita, Elis C. A. Eleutherio, Bianca C. Neves
Article
Multidisciplinary Sciences
Aline de Araujo Brasil, Mariana Dias Castela de Carvalho, Ellen Gerhardt, Daniela Dias Queiroz, Marcos Dias Pereira, Tiago Fleming Outeiro, Elis Cristina Araujo Eleutherio
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Elis Cristina Araujo Eleutherio, Rayne Stfhany Silva Magalhaes, Aline de Araujo Brasil, Jose Raphael Monteiro Neto, Luan de Holanda Paranhos
Summary: During cellular respiration, radicals like superoxide can cause cell damage, but cells use the enzyme Cu/Zn Superoxide Dismutase (SOD1) to convert superoxide into oxygen and hydrogen peroxide. In addition to its primary function, SOD1 also plays other roles, such as activating gene transcription or functioning as an RNA binding protein.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Rayne S. S. Magalhaes, Fernanda C. Boechat, Aline A. Brasil, Jose R. M. Neto, Gabriela D. Ribeiro, Luan H. Paranhos, Natalia Neves de Souza, Tuane Vieira, Tiago F. Outeiro, Bianca C. Neves, Elis C. A. Eleutherio
Summary: The research suggests that T6P may offer a new paradigm for cancer treatment based on specific inhibition of HK2, playing a crucial role in the survival and proliferation of cancer cells.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jose R. Monteiro Neto, Gabriela D. Ribeiro, Rayne S. S. Magalhaes, Cristian Follmer, Tiago F. Outeiro, Elis C. A. Eleutherio
Summary: This study found a relationship between the formation of methylglyoxal (MGO) and the degeneration of motor neurons in Amyotrophic Lateral Sclerosis (ALS). The accumulation of MGO led to the aggregation of human SOD1WT (hSOD1WT), decreased activity, and reduced cell viability. Additionally, MGO treatment increased the presence of hSOD1WT in stress granules. These findings suggest that glycation may play a significant role in the pathologies of hSOD1WT and TDP-43 in sporadic ALS.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biotechnology & Applied Microbiology
Rayne S. S. Magalhaes, Blagovesta Popova, Gerhard H. Braus, Tiago F. Outeiro, Elis C. A. Eleutherio
FEMS YEAST RESEARCH
(2018)