Article
Immunology
Gaurav Chandra Gyanwali, Thilina U. B. Herath, Antonella Gianfelice, Keith Ireton
Summary: The bacterial pathogen Listeria monocytogenes induces internalization by interacting with the host membrane-trafficking process of polarized exocytosis through its surface protein InIA and the human cell-cell adhesion molecule E-cadherin.
INFECTION AND IMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Keith Ireton, Gaurav Chandra Gyanwali, Thilina U. B. Herath, Nicole Lee
Summary: Intracellular bacterial pathogens remodel the plasma membrane of eukaryotic cells to establish infection by manipulating actin cytoskeleton and polarized exocytosis. Several bacterial pathogens exploit the host vesicular trafficking pathway of polarized exocytosis, mediated by the exocyst protein complex, to expand and reshape specific regions in the plasma membrane. They also manipulate exocyst components to promote internalization or intercellular spread within host tissues, modify membrane vacuoles, and favor intracellular replication or motility. Coordinating actin polymerization and polarized exocytosis is crucial for optimal bacterial infection.
MOLECULAR MICROBIOLOGY
(2023)
Article
Microbiology
Panji Cahya Mawarda, Cyrus A. Mallon, Xavier Le Roux, Jan Dirk van Elsas, Joana Falcao Salles
Summary: Microbial diversity can restrict the invasion and impact of alien microbes into soils via resource competition. Spore-forming bacteria with better resource use capacity are more likely to survive and affect bacterial community niches, especially in less diverse communities.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Paz Saldias, Pablo Cruz, Ian Silva, Octavio Orellana-Serradell, Boris Lavanderos, Diego Maureira, Raquel Pinto, Oscar Cerda
Summary: Triple-negative breast cancer has a poor prognosis and lacks response to traditional therapies, necessitating the development of new treatment strategies. Enhanced store-operated Ca2+ entry (SOCE) has been associated with tumorigenic behavior in breast cancer cells. In this study, we investigated the potential antitumor effects of a C-terminal SARAF fragment and found that its overexpression decreased the proliferation, migration, and invasion of breast cancer cells by reducing the SOCE response. These findings suggest that targeting the SOCE response via SARAF activity may offer alternative therapeutic options for triple-negative breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Sangsang Zhu, Chao Quan, Ruizhen Wang, Derong Liang, Shu Su, Ping Rong, Kun Zhou, Xinyu Yang, Qiaoli Chen, Min Li, Qian Du, Jingzi Zhang, Lei Fang, Hong-Yu Wang, Shuai Chen
Summary: RalGAP alpha 1-RalA signal nexus plays a key role in regulating calcium homeostasis in cardiomyocytes through the calcium pump SERCA2a, which helps maintain cardiac function under pressure overload conditions.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Chengcheng Liu, Kendall Stocke, Zackary R. Fitzsimonds, Lan Yakoumatos, Daniel P. Miller, Richard J. Lamont
Summary: The bacteria Porphyromonas gingivalis can deliver a tyrosine phosphatase, Ltp1, within epithelial cells, destabilizing PTEN and increasing RGCC production, leading to enhanced epithelial cell migration, proliferation, EMT, and inflammatory cytokine production. The disruption of the Akt1/RGCC signaling axis by Ltp1 facilitates P. gingivalis-induced pathogenic effects, contributing to oral bacterial diseases at mucosal barriers. The absence of Ltp1 leads to reduced pathogenicity in a murine model of periodontitis.
Article
Plant Sciences
S. J. Sapsford, I. A. Dickie
Summary: Biological invasions of plants have significant effects on ecosystem functioning by altering soil microbiota. This study investigated the recovery of soil enzymatic function following the removal of invasive Pinus spp. in New Zealand. The results showed that soil enzymatic activities were influenced by pine legacies through changes in soil properties and biotic communities. The slow recovery of enzymatic function may have implications for plant establishment and ecosystem restoration.
JOURNAL OF ECOLOGY
(2023)
Article
Biochemical Research Methods
Yushuang Liu, Shuping Jin, Hongli Gao, Xue Wang, Congjing Wang, Weifeng Zhou, Bin Yu
Summary: This article proposes a novel method called ML-locMLFE, which can effectively predict the multi-label subcellular localization of proteins and has obvious advantages. By using different feature extraction methods and information processing methods, this method demonstrates good accuracy in predicting the protein localization of diseases such as SARS-CoV-2.
Article
Environmental Sciences
Lucie A. Malard, Maria-Luisa Avila-Jimenez, Julia Schmale, Lewis Cuthbertson, Luke Cockerton, David A. Pearce
Summary: Parts of the Antarctic are undergoing dramatic ecosystem changes, increasing the risk of biological invasions. The study found that bacterial diversity in the Southern Ocean air has both local and global origins, and is significantly influenced by meteorological conditions.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Lei Pang, Zhiming Ma, Xi Zhang, Yuanzhi Huang, Ruili Li, Yansong Miao, Ruixi Li
Summary: Delivery of proteins to the plasma membrane in plants involves the RABA2a-SNARE pathway, which is independent of the exocyst-mediated secretion pathway. This plant-specific pathway is essential for maintaining potassium homeostasis in Arabidopsis seedlings and may have evolved as a response to environmental changes.
Article
Ecology
Alexandre R. T. Figueiredo, Ozhan Ozkaya, Rolf Kummerli, Jos Kramer
Summary: The study reveals that siderophores play a significant role in shaping invasion dynamics in bacterial communities.
Article
Biochemistry & Molecular Biology
Cristina Ciampelli, Grazia Galleri, Silvia Puggioni, Milena Fais, Lucia Iannotta, Manuela Galioto, Marta Becciu, Elisa Greggio, Roberto Bernardoni, Claudia Crosio, Ciro Iaccarino
Summary: Pathological mutations in the LRRK2 gene are a major genetic cause of Parkinson's disease (PD), and they are linked to vesicle dynamics control. The study shows that LRRK2 increases the assembly of the exocyst complex through Sec8 interaction, and inhibition of the exocyst complex can rescue the pathogenic phenotype of mutant LRRK2. Prolonged exocyst inhibition leads to a reduction in LRRK2 protein level, supporting the role of the exocyst complex in the LRRK2 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Lukas Synek, Roman Pleskot, Juraj Sekeres, Natalia Serrano, Nemanja Vukasinovic, Jitka Ortmannova, Martina Klejchova, Premysl Pejchar, Klara Batystova, Malgorzata Gutkowska, Edita Jankova-Drdova, Vedrana Markovic, Tamara Pecenkova, Jiri Santrucek, Viktor Zarsky, Martin Potocky
Summary: The study reveals plant-specific features of the exocyst complex and emphasizes the importance of the specific protein-lipid code for the recruitment of peripheral membrane proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Gale G. Bozzo, Nicole Unterlander
Summary: This review discusses the biological roles and degradation pathways of flavonol glycosides in plants, with a particular emphasis on the evidence for flavonol-specific beta-glucosidases. It highlights the challenges for identifying hypothetical plant alpha-rhamnosidases and peroxidases involved in flavonol glycoside degradation, as well as the potential biological role of this catabolism in mitigating oxidative stress in developing and postharvest plant tissues.
Article
Ecology
Mingshan Xu, Anna Yang, Xiaodong Yang, Wenting Cao, Zengke Zhang, Zengyan Li, Yu Zhang, Huaguo Zhang, Wenhui You, En-Rong Yan, David A. Wardle
Summary: Biodiversity on island ecosystems is influenced by human-driven land use change and biological invasion, as well as the direct and indirect effects of island size and remoteness. This study explores these relationships by analyzing the diversity of plants and soil bacteria on 37 continental shelf islands in eastern China.
FUNCTIONAL ECOLOGY
(2023)
Article
Biology
Suzanne E. Osborne, John H. Brumell
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2017)
Article
Cell Biology
Kirsten C. Boddy, Aggie Duan Gao, Dorothy Truong, Moshe S. Kim, Carol D. Froese, William S. Trimble, John H. Brumell
CELLULAR MICROBIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Ju Huang, Grace Y. Lam, John H. Brumell
ANTIOXIDANTS & REDOX SIGNALING
(2011)
Editorial Material
Cell Biology
Shahab Shahnazari, Anton Namolovan, Daniel J. Klionsky, John H. Brumell
Article
Cell Biology
Marija Cemma, Peter Kijun Kim, John Hunter Brumell
Editorial Material
Microbiology
Shahab Shahnazari, John H. Brumell
CELL HOST & MICROBE
(2009)
Editorial Material
Microbiology
John Hunter Brumell
CELL HOST & MICROBE
(2012)
Review
Biochemistry & Molecular Biology
Marija Cemma, John H. Brumell
Editorial Material
Multidisciplinary Sciences
Ju Huang, John H. Brumell
Article
Microbiology
Vanessa M. D'Costa, Etienne Coyaud, Kirsten C. Boddy, Estelle M. N. Laurent, Jonathan St-Germain, Taoyingnan Li, Sergio Grinstein, Brian Raught, John H. Brumell
NATURE MICROBIOLOGY
(2019)
Article
Immunology
Jorge David Rojas Marquez, Taoyingnan Li, Adam R. R. McCluggage, Joel M. J. Tan, Aleixo Muise, Darren E. Higgins, John H. Brumell
Summary: The study reveals a novel antimicrobial role for NOX2 in controlling bacterial dissemination through modulation of type I interferon responses. Deficiency in NOX2 leads to increased expression of interferon-stimulated genes and results in promotion of cell-to-cell spread by L. monocytogenes, defective leukocyte recruitment to infection foci, and production of anti-inflammatory effectors.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Cell Biology
Bing-Ru Yan, Taoyingnan Li, Etienne Coyaud, Estelle M. N. Laurent, Jonathan St-Germain, Yuhuan Zhou, Peter K. Kim, Brian Raught, John H. Brumell
Summary: The protein C5orf51 plays a crucial role in regulating the activity of RAB7A during mitophagy, facilitating the shuttle of RAB7A between late endosomes and mitochondria. Depletion of C5orf51 leads to compromised localization of RAB7A on depolarized mitochondria, degradation by the proteasome, and inhibition of ATG9A recruitment to depolarized mitochondria.
Article
Cell Biology
Kirsty M. Hooper, Elise Jacquin, Taoyingnan Li, Jonathan M. Goodwin, John H. Brumell, Joanne Durgan, Oliver Florey
Summary: This study reveals the important role of V-ATPase in non-canonical autophagy, as it is sufficient to activate CASM. V-ATPase recruits ATG16L1 during LAP, STING- and drug-induced CASM, indicating a common mechanism. Key molecular players, including NADPH oxidase/ROS, converge on V-ATPase during LAP. The study also demonstrates the contribution of CASM to the Salmonella host response and the disruption of V-ATPase-ATG16L1 axis by SopF.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Hematology
Sourav Bhattacharya, Rachel A. Idol, Wei Yang, Jorge David Rojas Marquez, Yanan Li, Guangming Huang, Wandy L. Beatty, Jeffrey J. Atkinson, John H. Brumell, Juhi Bagaitkar, Jeffrey A. Magee, Mary C. Dinauer
Summary: NOX2 plays a role in maintaining lung homeostasis and suppressing alveolar macrophage activation. The absence of NOX2 leads to enhanced cytokine responses in macrophages and lung inflammation.
