Article
Immunology
Fabio Fiorino, Elena Pettini, Oliver Koeberling, Annalisa Ciabattini, Gianni Pozzi, Laura B. Martin, Donata Medaglini
Summary: STm vaccine based on GMMA technology can induce long-lasting anti-bacterial immunity in mice and have a preventive effect on iNTS disease.
Article
Food Science & Technology
Rui Dong, Xiaojie Qin, Shoukui He, Xiujuan Zhou, Yan Cui, Chunlei Shi, Yiping He, Xianming Shi
Summary: The study revealed that DsrA plays a crucial role in promoting oxidative stress resistance in S. Typhimurium by regulating the expression of antioxidant enzymes and maintaining intracellular redox balance.
Article
Food Science & Technology
Zhongyue Ren, Lingling Peng, Shufang Chen, Yi Pu, Huihui Lv, Hua Wei, Cuixiang Wan
Summary: Salmonella Typhimurium is commonly found in food and can cause infection in the gastrointestinal tract. This study investigated the effect of a high-fat diet (HFD) on S. Typhimurium infection and the potential protective effects of Lactiplantibacillus plantarum 1201 intervention. The results showed that HFD exacerbated the infection, while intervention with L. plantarum 1201 effectively reduced colonization and inflammation. These findings suggest that L. plantarum 1201 may be a potential preventive strategy against S. Typhimurium infection.
Article
Microbiology
Yu Han, Yu Li, Zhiwei Zeng, Wenjing Li, Saixiang Feng, Weisheng Cao
Summary: This study explores the resistance mechanism and activity of the antimicrobial peptide MccY against Salmonella strains. The deletion of Ton system genes resulted in resistance to MccY and mutants showed increased susceptibility to MccY under a low-iron condition. MccY may decrease the virulence of S. Typhimurium by altering multiple physiological properties.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Na Sun, Yanying Song, Cong Liu, Mengda Liu, Lanping Yu, Fangkun Wang
Summary: This study aimed to clarify the mechanism of Salmonella survival and reproduction in the host by analyzing differentially expressed genes through high-throughput sequencing. A total of 1340 significant DEGs were screened, with functional annotation revealing their roles in regulation, metabolism, transport, binding, pathogenesis, and motility. Among the upregulated DEGs, 26 were identified as candidate key genes for survival and proliferation in vivo.
Article
Chemistry, Analytical
Han Du, Xu Zhang, Mingru Yao, Qingli Yang, Wei Wu
Summary: This study successfully constructed aptamer-guided luminous microspheres using carbon dots fluorescence, which can efficiently recognize and detect Salmonella enterica serovar Typhimurium. The method shows high sensitivity and has broad application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Microbiology
Jingyan Shu, Hongtao Liu, Yang Liu, Xindi Chen, Yu Yu, Qianghua Lv, Jianfeng Wang, Xuming Deng, Zhimin Guo, Jiazhang Qiu
Summary: Tannic acid (TA) has been shown to improve survival rates and alleviate cecal pathological lesions in S. Typhimurium-infected mice, while inhibiting bacterial invasion of HeLa cells. TA may exert its anti-infection effects by controlling the expression of key genes in the T3SS.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Stephanie Paulini, Florian D. Fabiani, Anna S. Weiss, Ana Laura Moldoveanu, Sophie Helaine, Baerbel Stecher, Kirsten Jung
Summary: Pyruvate, the simplest of the alpha-keto acids, plays a crucial role in the metabolic pathways of bacteria and eukaryotes. This study identified two specific transporters, BtsT and CstA, in Salmonella enterica serovar Typhimurium, which are important for pyruvate uptake and have significant effects on bacterial survival, infection, and persistence.
Article
Microbiology
Jiwon Baek, Hyunjin Yoon
Summary: Cyclic di-GMP is a signaling molecule that controls the transition between motile and nonmotile lifestyles in bacteria. It regulates biofilm formation and dispersal, and it can alter carbon metabolic pathways in Salmonella enterica serovar Typhimurium.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
R. Kavi Bharathi, C. S. Srinandan, N. Sai Subramanian
Summary: This study investigates the cooperative ability and matrix sharing between specialist and generalist strains during biofilm formation. The results show that the matrix aids in survival during antibiotic, chlorine, and predatory stress, and possible sharing of the matrix occurs in co-culture.
Article
Microbiology
Seul Kim, Eunsuk Kim, Hyunjin Yoon
Summary: Salmonella alters cellular processes to improve intracellular fitness during host infection by rewiring cellular transcriptional regulation through alternative sigma factors such as rpoS. Deprivation of outer membrane protein YcfR in Salmonella Typhimurium leads to decreased intracellular survival and downregulation of SPI-2 genes, which is abolished in the absence of rpoS. RpoS-associated stress responses in Salmonella due to impaired envelope integrity may reciprocally downregulate the expression of SPI-2 genes to reduce its virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Petra Geiser, Maria Letizia Di Martino, Pilar Samperio Ventayol, Jens Eriksson, Eduardo Sima, Anas Kh. Al-Saffar, David Ahl, Mia Phillipson, Dominic-Luc Webb, Magnus Sundbom, Per M. Hellstrom, Mikael E. Sellin
Summary: This study reveals the complete infection cycle of Salmonella enterica serovar Typhimurium within human and mouse enteroids, and demonstrates how bacterial invasion of the epithelium strongly fuels expansion in the luminal compartment through a mechanism involving the death and expulsion of bacterium-infected epithelial cells.
Article
Microbiology
Yuan Yuan, Yara Seif, Kevin Rychel, Reo Yoo, Siddharth Chauhan, Saugat Poudel, Tahani Al-bulushi, Bernhard O. Palsson, Anand Sastry
Summary: By combining pan-genome and transcriptomic analytics, variations in transcriptional regulatory networks across six strains of Salmonella enterica serovar Typhimurium were revealed.
Article
Food Science & Technology
Hui Li, Keren Agyekumwaa Addo, Yi-gang Yu, Xing-long Xiao
Summary: The study investigated the influence of cuminaldehyde (CUM) on cell membrane and genomic DNA of Salmonella enterica serovar Typhimurium (S. Typhimurium), and revealed its antibacterial mechanism. CUM could bind to bacterial membrane proteins and change its structure and conformation, leading to the leakage of intracellular substances and the destruction of cell integrity. DNA interaction analysis showed that CUM bound to genomic DNA through groove mode, affecting the normal functioning of S. Typhimurium.
Article
Nutrition & Dietetics
Weihua Tan, Yuanyuan Tian, Qingya Zhang, Siwei Miao, Wenrong Wu, Xiaoqing Miao, Haiou Kuang, Wenchao Yang
Summary: In this study, it was found that Apis laboriosa honey (ALH) harvested in China has antibacterial activity against Salmonella enterica serovar Typhimurium (S. Typhimurium). The physicochemical parameters and antioxidant activity of ALH were influenced by the time and region of harvest. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ALH against S. Typhimurium were similar to UMF5+ manuka honey. Proteomic experiment revealed the antibacterial mechanism of ALH.
FRONTIERS IN NUTRITION
(2023)
Article
Biology
Shixuan Liu, Miriam Bracha Ginzberg, Nish Patel, Marc Hild, Bosco Leung, Zhengda Li, Yen-Chi Chen, Nancy Chang, Yuan Wang, Ceryl Tan, Shulamit Diena, William Trimble, Larry Wasserman, Jeremy L. Jenkins, Marc W. Kirschner, Ran Kafri
Article
Cell Biology
Adriano Vissa, Maximiliano Giuliani, Carol D. Froese, Moshe S. Kim, Forooz Soroor, Peter K. Kim, William S. Trimble, Christopher M. Yip
Article
Cell Biology
Pedro E. Marques, Steffen Nyegaard, Richard F. Collins, Fulvia Troise, Spencer A. Freeman, William S. Trimble, Sergio Grinstein
DEVELOPMENTAL CELL
(2019)
Article
Cell Biology
Roni Levin-Konigsberg, Fernando Montano-Rendon, Tal Keren-Kaplan, Ren Li, Braeden Ego, Sivakami Mylvaganam, Jessica E. DiCiccio, William S. Trimble, Michael C. Bassik, Juan S. Bonifacino, Gregory D. Fairn, Sergio Grinstein
NATURE CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Saskia Heybrock, Kristiina Kanerva, Ying Meng, Chris Ing, Anna Liang, Zi-Jian Xiong, Xialian Weng, Young Ah Kim, Richard Collins, William Trimble, Regis Pomes, Gilbert G. Prive, Wim Annaert, Michael Schwake, Joerg Heeren, Renate Luellmann-Rauch, Sergio Grinstein, Elina Ikonen, Paul Saftig, Dante Neculai
NATURE COMMUNICATIONS
(2019)
Article
Microbiology
Vanessa M. D'Costa, Etienne Coyaud, Kirsten C. Boddy, Estelle M. N. Laurent, Jonathan St-Germain, Taoyingnan Li, Sergio Grinstein, Brian Raught, John H. Brumell
NATURE MICROBIOLOGY
(2019)
Article
Cell Biology
Tara Akhshi, William S. Trimble
Summary: The study reveals that Smo induces ciliogenesis through two distinct pathways while also activating autophagy, with the Gα(i)-LGN-NuMA-dynein complex playing a crucial role in the process. Different Smo agonists activate these pathways separately, and only together they can replicate the activity of Hh ligand.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Immunology
Jorge David Rojas Marquez, Taoyingnan Li, Adam R. R. McCluggage, Joel M. J. Tan, Aleixo Muise, Darren E. Higgins, John H. Brumell
Summary: The study reveals a novel antimicrobial role for NOX2 in controlling bacterial dissemination through modulation of type I interferon responses. Deficiency in NOX2 leads to increased expression of interferon-stimulated genes and results in promotion of cell-to-cell spread by L. monocytogenes, defective leukocyte recruitment to infection foci, and production of anti-inflammatory effectors.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Cell Biology
Bing-Ru Yan, Taoyingnan Li, Etienne Coyaud, Estelle M. N. Laurent, Jonathan St-Germain, Yuhuan Zhou, Peter K. Kim, Brian Raught, John H. Brumell
Summary: The protein C5orf51 plays a crucial role in regulating the activity of RAB7A during mitophagy, facilitating the shuttle of RAB7A between late endosomes and mitochondria. Depletion of C5orf51 leads to compromised localization of RAB7A on depolarized mitochondria, degradation by the proteasome, and inhibition of ATG9A recruitment to depolarized mitochondria.
Article
Biochemistry & Molecular Biology
Tara Akhshi, Rachel Shannon, William S. Trimble
Summary: Hedgehog (Hh) signaling plays critical roles in development and cell homeostasis. Canonical and non-canonical Hh signaling are interlinked and can overlap spatially and functionally.
Article
Cell Biology
Kirsty M. Hooper, Elise Jacquin, Taoyingnan Li, Jonathan M. Goodwin, John H. Brumell, Joanne Durgan, Oliver Florey
Summary: This study reveals the important role of V-ATPase in non-canonical autophagy, as it is sufficient to activate CASM. V-ATPase recruits ATG16L1 during LAP, STING- and drug-induced CASM, indicating a common mechanism. Key molecular players, including NADPH oxidase/ROS, converge on V-ATPase during LAP. The study also demonstrates the contribution of CASM to the Salmonella host response and the disruption of V-ATPase-ATG16L1 axis by SopF.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Hematology
Sourav Bhattacharya, Rachel A. Idol, Wei Yang, Jorge David Rojas Marquez, Yanan Li, Guangming Huang, Wandy L. Beatty, Jeffrey J. Atkinson, John H. Brumell, Juhi Bagaitkar, Jeffrey A. Magee, Mary C. Dinauer
Summary: NOX2 plays a role in maintaining lung homeostasis and suppressing alveolar macrophage activation. The absence of NOX2 leads to enhanced cytokine responses in macrophages and lung inflammation.
Article
Biochemistry & Molecular Biology
Oliva Palander, Adam Lam, Richard F. Collins, Theo J. Moraes, William S. Trimble
Summary: Primary cilia play crucial roles in several signaling pathways, and disorders in cilia function and formation can result in various diseases collectively referred to as ciliopathies. The DIAPH family of formins, including DIAPH1, DIAPH2, and DIAPH3, have been shown to be involved in ciliogenesis and cilia maintenance through actin polymerization and microtubule stability. Depletion of DIAPH2 or DIAPH3 disrupts ciliogenesis and cilia length, with DIAPH3 depletion specifically affecting trafficking within cilia. Targeting DIAPH3 to the basal body increases trafficking to the ciliary base, elongates cilia, and forms bulbs at the tips, highlighting the importance of DIAPH proteins in regulating cilia maintenance.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)