Review
Biochemistry & Molecular Biology
Eleanor Dickson-Murray, Kenza Nedara, Nazanine Modjtahedi, Kostas Tokatlidis
Summary: Mitochondrial proteins, mostly encoded by nuclear genes, must be precisely targeted and sorted within mitochondria. Protein translocases facilitate this process, with the main import pathway for the intermembrane space chemically modifying imported proteins by introducing disulfide bonds. Mia40 is a key component in this pathway, regulated by redox processes, and plays a crucial role in trapping folded precursors in the intermembrane space.
Review
Biochemistry & Molecular Biology
Ruairidh Edwards, Ross Eaglesfield, Kostas Tokatlidis
Summary: The mitochondrial intermembrane space (IMS) is a narrow sub-mitochondrial compartment with diverse protein import mechanisms. IMS proteins do not require energy to cross the outer mitochondrial membrane, unlike import into the matrix or inner membrane. Many IMS proteins are associated with human diseases, highlighting the importance of understanding their normal function and localization in the IMS.
Article
Biochemistry & Molecular Biology
Koichi Fujisawa, Maina Wakazaki, Aya Matsuzaki, Toshihiko Matsumoto, Naoki Yamamoto, Takafumi Noma, Taro Takami
Summary: The balance between oxidative phosphorylation and glycolysis is crucial for cancer cell growth and survival. In this study, the researchers focused on adenylate kinase 3 (AK3), an enzyme in the mitochondrial matrix that regulates adenine nucleotide metabolism. The knockout of AK3 in HeLa cells resulted in reduced proliferation and increased G1 phase cells. Metabolomic analysis showed decreased ATP levels and changes in glycolytic and TCA cycle metabolites. Additionally, AK3KO cells exhibited increased mitochondrial DNA and oxidative stress levels. The study concluded that increased expression of PCK2 may compensate for the deficiency of GDP caused by AK3 knockout.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maithili Saoji, Aditya Sen, Rachel T. Cox
Summary: The study revealed the subtle functions of mtRNase P in vivo, showing that the loss of a specific member can lead to different cleavage effects, which may improve the understanding of human diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Taigo Horiguchi, Ayako Tanimura, Keiko Miyoshi, Hiroko Hagita, Hisanori Minami, Takafumi Noma
Summary: This study establishes a model using CRISPR/Cas9 technology and reveals that AK2 deficiency leads to reticular dysgenesis and impairs neutrophil differentiation. Mutations in the N-terminal region of AK2 may cause structural changes. Fructose may be a potential treatment for AK2 deficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Leenu Reinsalu, Marju Puurand, Vladimir Chekulayev, Sten Miller, Igor Shevchuk, Kersti Tepp, Egle Rebane-Klemm, Natalja Timohhina, Anton Terasmaa, Tuuli Kaambre
Summary: Metabolic plasticity is the ability of cells to adjust metabolism to environmental changes. Cancer cells exhibit increased metabolic plasticity, allowing them to have a survival advantage and higher proliferative capacity. This enhanced plasticity is characterized by changes in adenine nucleotide transport, impaired regulation of VDAC and reprogrammed energy transfer circuits like AK and CK pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Infectious Diseases
Guoqing Shao, Ruiqi Hua, Hongyu Song, Yanxin Chen, Xiaowei Zhu, Wei Hou, Shengqiong Li, Aiguo Yang, Guangyou Yang
Summary: The study demonstrates that the six recombinant proteins have significant protective efficacy in dogs against E. granulosus infection, reducing worm biomass and inhibiting growth. The vaccinated dogs also showed an increased level of IgG, indicating an enhanced immune response.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Multidisciplinary Sciences
Qingqing Sun, Xueli Cao, Zihe Liu, Chuanjing An, Jinglei Hu, Yue Wang, Meiyu Qiao, Teng Gao, Wenzhen Cheng, Yi Zhang, Yue Feng, Hongbo Gao
Summary: Chloroplast division involves complex protein interactions, with PDV1 playing a crucial role in stabilizing PARC6. Under light conditions, reduction of a disulfide bond within PARC6 promotes its interaction with PDV1, ultimately leading to chloroplast division. Magnesium ions also enhance PARC6 dimerization, further contributing to the regulation of chloroplast division.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Qingqing Sun, Xueli Cao, Zihe Liu, Chuanjing An, Jinglei Hu, Yue Wang, Meiyu Qiao, Teng Gao, Wenzhen Cheng, Yi Zhang, Yue Feng, Hongbo Gao
Summary: Chloroplast division requires the coordination of protein complexes from stroma to cytosol. The interaction between PDV1 and PARC6, crucial for chloroplast division, is regulated by intramolecular disulfide bond formation in PARC6, which can be reduced by light exposure. Additionally, magnesium ions, which increase in chloroplasts upon light exposure, promote PARC6 dimerization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Marcel Morgenstern, Christian D. Peikert, Philipp Luebbert, Ida Suppanz, Cinzia Klemm, Oliver Alka, Conny Steiert, Nataliia Naumenko, Alexander Schendzielorz, Laura Melchionda, Wignand W. D. Muehlhaeuser, Bettina Knapp, Jakob D. Busch, Sebastian B. Stiller, Stefan Dannenmaier, Caroline Lindau, Mariya Licheva, Christopher Eickhorst, Riccardo Galbusera, Ralf M. Zerbes, Michael T. Ryan, Claudine Kraft, Vera Kozjak-Pavlovic, Friedel Drepper, Sven Dennerlein, Silke Oeljeklaus, Nikolaus Pfanner, Nils Wiedemann, Bettina Warscheid
Summary: Researchers have classified and identified human mitochondrial proteins, establishing a high-confidence mitochondrial proteome containing over 1,100 proteins, which account for 7% of cellular proteins and are linked to various diseases.
Article
Biochemistry & Molecular Biology
Robert Kofler, Viola Nolte, Christian Schlotterer
Summary: The dynamics of transposable element invasions are influenced by multiple factors, and the transposition rate is only one of them. By studying the temperature-dependent activity of the P-element, researchers found that despite differences in transposition rates, the copy numbers of the P-element were very similar at different temperatures. The decrease in insertion rate as the copy number approached a plateau was accompanied by the production of piRNAs. However, fewer P-element insertions were observed in piRNA clusters than expected, indicating that factors other than the trap model influenced the dynamics of TE invasions.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Chemistry, Physical
Takumi Washio, Ryo Kanada, Xiaoke Cui, Jun-ichi Okada, Seiryo Sugiura, Shoji Takada, Toshiaki Hisada
Summary: The semi-implicit Hessian correction (SimHec) scheme proposed in this study allows for significantly larger time steps in molecular dynamics simulations compared to traditional implicit methods. By introducing a hybrid method (SimHec-H) that combines explicit time steps, the drawbacks of increased transition dynamics and reduced fluctuations in bonded potentials can be corrected. This scheme results in a speedup factor of 7-30, making simulations more efficient and accurate.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2021)
Article
Biochemistry & Molecular Biology
Flavien Zannini, Johannes M. Herrmann, Jeremy Couturier, Nicolas Rouhier
Summary: Protein import and oxidative folding within the intermembrane space of mitochondria rely on the MIA40-ERV1 couple. While MIA40 is essential in most eukaryotes, it is dispensable in Arabidopsis thaliana due to the presence of glutathione, which supports the oxidation of MIA40 substrates by AtERV1.
Review
Biochemistry & Molecular Biology
Leszek A. Kleczkowski, Abir U. Igamberdiev
Summary: Free magnesium (Mg2+) functions as a signal of adenylate (ATP+ADP+AMP) status in cells, controlled by the equilibrium of adenylate kinase (AK) and membrane-bound adenylate- and Mg2+-translocators. Changes in [Mg2+] impact enzyme activities, metabolism, and processes like photosynthesis, under normal and stress conditions. Magnesium signaling is likely widespread in all eukaryotic and prokaryotic organisms due to the ubiquitous nature of AK and its role in adenylate equilibration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chizuru Ito, Tsukasa Makino, Tohru Mutoh, Masahide Kikkawa, Kiyotaka Toshimori
Summary: Normal sperm flagellar shape and movement are crucial for fertilization. The proteins ODF4, AK1, and AK2 play important roles in the morphology and function of sperm flagella. Deletion of ODF4 leads to abnormal flagellar shape and movement, resulting in male infertility. This study demonstrates the relationship between ODF4, AK1, and AK2 in regulating flagellar structure and function.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Fuqiang Ge, Qiaoling Yu, Jun Zhang, Yaoyao Han, Dongfa Zhu, Xi Xie
Summary: The E93 gene was identified and found to be widely distributed in adult tissues of the swimming crab. The expression levels of PtE93 mRNA in Y-organ and epidermis fluctuated during the molt cycle, suggesting its involvement in juvenile molting. Treatment with 20E induced PtE93 expression, while treatment with MF showed the opposite effect. The study also observed a negative correlation between PtE93 and ecdysteroid biosynthesis genes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Manuel Marrero, Oscar Monroig, Jose A. Perez, Monica B. Betancor, Ana Galindo, Ana Bolanos, N. Guadalupe Acosta, Covadonga Rodriguez
Summary: This study investigated the combined effects of partial dietary replacement of fish oil (FO) by vegetable oil (VO) and reduced ambient salinity on the fatty acid composition of C. labrosus juveniles. The results showed that C. labrosus is able to biosynthesise EPA and DHA from ALA, and low salinity conditions can upregulate the gene expression related to fatty acid metabolism. Fish reared on FO20 diet showed the highest amount of n-3 LC-PUFA in muscle.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Davide Degli Esposti, Aureline Lalouette, Karen Gaget, Louveline Lepeule, Zineb Chaabi, Maxime Lepretre, Anabelle Espeyte, Nicolas Delorme, Herve Queau, Laura Garnero, Federica Calevro, Arnaud Chaumot, Olivier Geffard
Summary: Metal pollution has significant effects on aquatic environments, and metallothioneins play important roles in maintaining metal homeostasis and detoxification. This study identified two metallothionein-coding transcripts in Gammarus fossarum and investigated their organ-specific expression patterns. The results showed that mt1 was more strongly induced after cadmium exposure, and mt1 was more highly induced in the caeca compared to the gills for any metal exposure. Additionally, mt2 was more inducible in the gills than in the caeca for cadmium and zinc exposure at the individual level.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cuihong You, Shuangshuang Miao, Zhiyong Xie, Siyuan Lin, Shuqi Wang, Cuiying Chen, Li Lin, Yanhua Huang, Meng Zhou, Yewei Dong, Yuanyou Li, Ping Zhuang
Summary: The rabbitfish S. guttatus was found to have the capability for LC-PUFA biosynthesis, with the expression of related genes varying temporally and spatially during embryogenesis and being influenced by ambient salinity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Saydur Rahman, Mohammad Maruf Billah, Victor Rangel, Esmirna Cantu
Summary: Global warming has severe impacts on the reproductive fitness of marine invertebrates. In this study, the researchers used various techniques to investigate the effects of high temperatures on global DNA methylation, cellular apoptosis, and nitrative stress in the gonads of Atlantic sea urchins.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
E. S. Kladchenko, A. A. Tkachuk, M. S. Podolskaya, A. Yu. Andreyeva
Summary: This study investigated the osmotic stability and cellular responses of hemocytes in Mediterranean mussel and Pacific oyster. The results revealed that both species showed cellular volume changes and alterations in mitochondrial membrane potential upon hypoosmotic swelling. These findings provide insights into the cellular responses of bivalve mollusks to osmotic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yuya Ohhara, Chiemi Sagisaka, Kimiko Yamakawa-Kobayashi
Summary: This study investigated the biosynthetic capacity of n-3 PUFAs in the terrestrial invertebrate Sinella dubiosa, and found that it can metabolize oleic, linolenic, and arachidonic acids to produce EPA, suggesting that it may serve as an EPA source in soil ecosystems.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Correction
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
Summary: This study provides the first report on the digestive tract pH of the pygmy octopus. The pH of the digestive tract regions was found to be lower than 7.0 and did not differ significantly between wild and captive octopuses. The digestive gland had the lowest pH values, but they were still above 5.0.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Carlos Espirito-Santo, Carmen Alburquerque, Francisco A. Guardiola, Rodrigo O. A. Ozorio, Leonardo J. Magnoni
Summary: Swimming has physiological effects on farmed fish, but the potential link between swimming and oxidative stress is still poorly understood. This study investigated the impact of different moderate swimming conditions on the antioxidant status of gilthead seabream, revealing that swimming modulated the activity of antioxidant enzymes in the liver and skeletal muscle. Adequate swimming conditions were found to minimize oxidative stress in gilthead seabream.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingxin Zhang, Chaobin Qin, Junli Wang, Liping Yang, Xiao Yan, Shaoyang Zhi, Guoxing Nie
Summary: The study focuses on the expression patterns of PFK genes in response to glucose, insulin, and glucagon stimuli in grass carp. The findings suggest that PFK family genes have different or even opposite expression patterns in various tissues, which may contribute to glucose intolerance in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jianshuang Li, Likang Lyu, Haishen Wen, Yun Li, Xiaojie Wang, Yijia Yao, Xin Qi
Summary: This study investigated the transcriptional regulation of estrogen receptors (ESRs) on isotocin receptors (itrs) in guppies, and found that estrogen can regulate oxytocin receptor expression in ovoviviparous teleosts. The study also demonstrated that ESR1 represses itr2 promoter activity in an estrogen-dependent manner, while overexpression of ESR2a or ESR2b enhances itr2 promoter activity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Wan-Xin Li, Li-Ting Cai, Yan-Ping Huang, Yuan-Qing Huang, Shu-Hui Pan, Zhi-Long Liu, Charles Brighton Ndandala, Gang Shi, Si-Ping Deng, Hong-Juan Shi, Guang-Li Li, Dong-Neng Jiang
Summary: In this study, it was found that S. argus has two lep genes, with lepa highly expressed in the liver and lepb not detected in any tissue. lepa was found to play a role in regulating female reproduction in S. argus.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Li-Xiang Wu, Xiao-Ying Tan, Yi-Chuang Xu, Hua Zheng, Xiao-Lei Wei, Wu-Hong Lv, Zhi Luo
Summary: This study investigates the mechanisms underlying the activation of lipophagy in yellow catfish under high glucose-induced lipid deposition. The SIRT1-NRF2-TFEB pathway is proposed to mediate lipophagy activation, and lipophagy helps alleviate high glucose-induced lipid deposition. This study provides insights into the control of lipophagy in response to metabolic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
