Article
Biochemistry & Molecular Biology
Jingxin Wang, Norikazu Saiki, Ayako Tanimura, Takafumi Noma, Akira Niwa, Tastutoshi Nakahata, Megumu K. Saito
Summary: The use of UK-5099 was found to improve neutrophil maturation defects in Reticular dysgenesis without affecting reactive oxygen species levels. This has potential therapeutic implications for treating this rare genetic disease.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Kendall J. Condon, Jose M. Orozco, Charles H. Adelmann, Jessica B. Spinelli, Pim W. van der Helm, Justin M. Roberts, Tenzin Kunchok, David M. Sabatini
Summary: Our study identified a catalog of genes that impact the mTORC1 pathway and clarified the multifaceted ways in which mTORC1 senses mitochondria dysfunction.AMPK and HRI are two of the key kinases that signal mitochondria distress to mTORC1, and their loss renders mTORC1 signaling largely resistant to mitochondria dysfunction induced by various inhibitors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell & Tissue Engineering
Liangqiang He, Yingzhe Ding, Yu Zhao, Karl K. So, Xianlu L. Peng, Yuying Li, Jie Yuan, Zhiming He, Xiaona Chen, Hao Sun, Huating Wang
Summary: Skeletal muscle satellite cells (SCs) are stem cells responsible for muscle development and regeneration. The study establishes a platform for editing endogenous SCs using the CRISPR/Cas9/AAV9-sgRNA system, revealing distinct functions of key regulators MYC and BCL6 in SC activation and muscle regeneration. Additionally, it is shown that MYC orchestrates SC activation through regulating 3D genome architecture, with its depletion affecting gene expression.
Article
Medicine, Research & Experimental
Qingxia Zuo, Wanqing Xu, Yanbin Wan, Dongyan Feng, Changsheng He, Cailing Lin, Dongchao Huang, Feng Chen, Liya Han, Qi Sun, Dong Chen, Hongli Du, Lizhen Huang
Summary: CRISPR/Cas9 was used to generate a CYP3A4-T2A-luciferase knock-in HepaRG subclone, and optimized differentiation procedures were found to promote differentiation and improve the expression of hepatocyte-specific genes.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Cell Biology
Jeffrey G. Shannon, B. Joseph Hinnebusch
Summary: Neutrophils derived from Hoxb8-ER-immortalized progenitors are functionally equivalent to primary neutrophils and can be genetically manipulated. They have similar morphology, gene expression, and antimicrobial abilities. These cells are valuable tools for studying bacterial pathogenesis.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Joao S. Teodoro, Ivo F. Machado, Ana C. Castela, Joao A. Amorim, Ivana Jarak, Rui A. Carvalho, Carlos M. Palmeira, Anabela P. Rolo
Summary: The study found that the bile acid CDCA can reverse obesity and accelerate metabolism, but this effect is not entirely dependent on thermogenesis; CDCA mainly reduces cellular obesity by promoting a healthy population of mitochondria, enhancing mitochondrial activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Lian Li, Jianqi Feng, Shanshan Zhao, Zhili Rong, Ying Lin
Summary: The study utilized gene editing technology to inactivate the transcription factor SOX9 in human embryonic stem cells, revealing that SOX9 modulates the proliferative capacity of lung epithelium but is not an indispensable factor in the regulation of human lung epithelium development.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biology
Justin A. Bosch, Berrak Ugur, Israel Pichardo-Casas, Jordan Rabasco, Felipe Escobedo, Zhongyuan Zuo, Ben Brown, Susan Celniker, David A. Sinclair, Hugo J. Bellen, Norbert Perrimon
Summary: Naturally produced peptides play important roles in regulating physiology, development, and metabolism. Recent studies suggest that thousands of peptides may be translated from transcripts containing small open-reading frames (smORFs). In Drosophila, two peptides encoded by conserved smORFs, Sloth1 and Sloth2, were found to have similar sequences but non-redundant functions. Loss of either peptide results in animal lethality, reduced neuronal function, impaired mitochondrial function, and neurodegeneration. These peptides are highly expressed in neurons, imported to mitochondria, and regulate mitochondrial complex III assembly.
Article
Cell Biology
Joseph Andrew Whitley, Sungjin Kim, Lei Lou, Chenming Ye, Omar Awad Alsaidan, Essilvo Sulejmani, Jingwen Cai, Ellison Gerona Desrochers, Zanna Beharry, Catherine Bowes Rickman, Mikael Klingeborn, Yutao Liu, Zhong-Ru Xie, Houjian Cai
Summary: CRISPR/Cas9 genome editing is a promising method for treating genetic diseases. However, encapsulating the CRISPR/Cas9 machinery for delivery is challenging. In this study, the researchers found that myristoylated proteins were preferentially encapsulated into extracellular vesicles (EVs). By fusing a myristoylated peptide onto the N-terminus of Cas9, they were able to promote the myristoylation and encapsulation of Cas9 into EVs. Additionally, introducing VSV-G increased the transfection potential. The encapsulated Cas9 accounted for 0.7% of total EV protein. The EVs coated with VSV-G encapsulating Cas9/sgRNA-eGFP showed up to 42% eGFP knock out efficiency in recipient cells, with limited off-target effects.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Immunology
Theresa Pinter, Maria Fischer, Markus Schaefer, Michaela Fellner, Julian Jude, Johannes Zuber, Meinrad Busslinger, Miriam Woehner
Summary: Plasma cells and plasmablasts are crucial for immune protection, and this study identified several new genes that are essential for plasmablast development through CRISPR-Cas9 screening.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Entomology
Fangying Yang, Zhongjie Zhang, Bo Hu, Ye Yu, Anjiang Tan
Summary: Recent research has identified a key role for the CCCH-type zinc finger gene Bmznf-2 in male sexual differentiation in Bombyx mori, with loss of its function resulting in male-to-female splicing variants and various phenotypic abnormalities.
Article
Biochemistry & Molecular Biology
Gen Yasui, Syouichi Katayama, Yukihiko Kubota, Hisashi Takatsuka, Masahiro Ito, Tetsuya Inazu
Summary: The study identified the MeCP2 binding protein ZNF483 as broadly conserved in metazoans and proposed its involvement in promoting neuronal differentiation by regulating the subcellular localization of MeCP2 in P19 cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Yuting Fu, Fangyuan Liu, Shuo Cao, Jia Zhang, Huizhi Wang, Baojiang Wu, Yongli Song, Shuguang Duo, Xihe Li, Siqin Bao
Summary: This study reveals that Bdh2 deficiency has no impact on pluripotency and self-renewal of ESCs, but promotes endoderm differentiation and is essential for maintaining DNA methylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Rui Hua, Sumin Gu, Jean X. Jiang
Summary: This study found that Cx43 hemichannels play a critical role in the differentiation of osteoblasts into osteocytes, and regulate osteoclast differentiation through secreted factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Carmen Martinez-Fernandez, Milana Bergamino, Alfonso Schiavi, David Brena, Natascia Ventura, Sebastian Honnen, Alberto Villanueva, Ernest Nadal, Julian Ceron
Summary: This study used the nematode Caenorhabditis elegans to investigate the effects of cisplatin on mitochondrial functions and neurotoxicity. It was found that a high-glucose diet sensitized the worms to cisplatin and that mitochondrial CED-13 protected cells from cisplatin-induced oxidative stress. In addition, dopamine was shown to have a protective role against cisplatin-induced neurotoxicity.
DISEASE MODELS & MECHANISMS
(2022)
Article
Cell & Tissue Engineering
Hiroshi Kondo, Keiko Miyoshi, Shoji Sakiyama, Akira Tangoku, Takafumi Noma
STEM CELLS INTERNATIONAL
(2015)
Article
Dentistry, Oral Surgery & Medicine
A. Adiningrat, A. Tanimura, K. Miyoshi, H. Hagita, R. D. Yanuaryska, D. Y. Arinawati, T. Horiguchi, T. Noma
Article
Cell Biology
Ayako Tanimura, Keiko Miyoshi, Taigo Horiguchi, Hiroko Hagita, Koichi Fujisawa, Takafumi Noma
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Biotechnology & Applied Microbiology
Dian Yosi Arinawati, Keiko Miyoshi, Ayako Tanimura, Taigo Horiguchi, Hiroko Hagita, Takafumi Noma
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2018)
Article
Multidisciplinary Sciences
Ayako Tanimura, Taigo Horiguchi, Keiko Miyoshi, Hiroko Hagita, Takafumi Noma
Article
Cell & Tissue Engineering
Naoya Kawakita, Hiroaki Toba, Keiko Miyoshi, Shinichi Sakamoto, Daisuke Matsumoto, Mika Takashima, Mariko Aoyama, Seiya Inoue, Masami Morimoto, Takeshi Nishino, Hiromitsu Takizawa, Akira Tangoku
STEM CELL RESEARCH & THERAPY
(2020)
Article
Physiology
Intan Ruspita, Pragnya Das, Yan Xia, Sarah Kelangi, Keiko Miyoshi, Takafumi Noma, Malcolm L. Snead, Rena N. D'Souza, Marianna Bei
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Cell Biology
Susumu Tadokoro, Reiko Tokuyama-Toda, Seiko Tatehara, Shinji Ide, Hirochika Umeki, Keiko Miyoshi, Takafumi Noma, Kazuhito Satomura
Summary: By culturing iPSCs on frozen sections with suitable microenvironments, they can be differentiated into specific cell lineages. The differentiation efficacy of iPSC clones varied significantly, suggesting that this induction method could be useful for evaluating iPSC quality.
Review
Dentistry, Oral Surgery & Medicine
Satoru Kisoda, Yasuhiro Mouri, Naoya Kitamura, Tetsuya Yamamoto, Keiko Miyoshi, Yasusei Kudo
Summary: Cervical lymph node metastasis is the most important prognostic factor in head and neck squamous cell carcinoma (HNSCC), where cancer cells invade the underlying stroma by breaching the basement membrane. The ability to metastasize is achieved through epithelial-to-mesenchymal transition (EMT), a biological process in which cells lose their epithelial features and gain mesenchymal features. Recent evidence highlights the intermediate state of partial-EMT and its higher aggressiveness compared to complete EMT. However, the specific role of partial-EMT remains unclear. This review summarizes the features of partial-EMT in HNSCC and discusses the therapeutic potential for targeting it.
JOURNAL OF ORAL BIOSCIENCES
(2022)
Article
Biology
Keiko Miyoshi, Hiroko Hagita, Taigo Horiguchi, Ayako Tanimura, Takafumi Noma
Summary: This study redefines the structure of the glucocerebrosidase coding gene and elucidates the regulatory mechanisms of its transcription and translation. The research discovers alternative uses of gene promoters in macrophages and neutrophils, as well as cap-independent translation through internal ribosome entry site activities. Furthermore, it reveals reciprocal expression between GBA and miR22-3p versus GBAP1 transcripts during macrophage differentiation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Taigo Horiguchi, Yumiko Miyatake, Keiko Miyoshi, Ayako Tanimura, Hiroko Hagita, Hiroshi Sakaue, Takafumi Noma
JOURNAL OF MEDICAL INVESTIGATION
(2020)
Article
Medicine, Research & Experimental
Ryna Dwi Yanuaryska, Keiko Miyoshi, Arya Adiningrat, Taigo Horiguchi, Ayako Tanimura, Hiroko Hagita, Takafumi Noma
JOURNAL OF MEDICAL INVESTIGATION
(2014)
Article
Medicine, Research & Experimental
Taigo Horiguchi, Miyuki Fuka, Koichi Fujisawa, Ayako Tanimura, Keiko Miyoshi, Ryutaro Murakami, Takafumi Noma
JOURNAL OF MEDICAL INVESTIGATION
(2014)
Article
Medicine, Research & Experimental
Arya Adiningrat, Ayako Tanimura, Keiko Miyoshi, Ryna Dwi Yanuaryska, Hiroko Hagita, Taigo Horiguchi, Takafumi Noma
JOURNAL OF MEDICAL INVESTIGATION
(2014)