Article
Microbiology
Yi-Yun Liu, Xiao-Qing Zhu, Sue C. Nang, Haoliang Xun, Luchao Lv, Jun Yang, Jian-Hua Liu
Summary: The prevalence of mcr-1 is higher in E. coli than in K. pneumoniae due to the greater transferability and persistence of mcr-1-bearing plasmids in the former species.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Abed Zahedi Bialvaei, Parisa Eslami, Leila Ganji, Alireza Dolatyar Dehkharghani, Farhad Asgari, Hossein Koupahi, Hamid Reza Barzegarian Pashacolaei, Mohammad Rahbar
Summary: This study investigated the molecular epidemiology of carbapenemase-producing and colistin-resistant clinical K. pneumoniae. The prevalence of resistance-associated genes was examined, and it was found that KPC enzyme was the most common carbapenemase identified. No NDM-1 gene was detected, but a mutation in the mgrB gene was observed in a high proportion of isolates. It is important to enhance surveillance, follow infection prevention protocols, and practice antibiotic stewardship to control the spread of resistant K. pneumoniae.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Shumei Huang, Shuang Wang, Yan Li, Ming Fang, Zengqiang Kou, Baoli Chen, Liuchen Xu, Zhenwang Bi, Hao Xu, Xiaohui Chi, Zhenqiang Bi
Summary: This study found that colistin-resistant Escherichia coli, most of which are multi-drug resistant strains, is common and highly transmissible among healthy residents in rural areas in China. Interventions should be implemented to prevent the spread of colistin-resistant Escherichia coli through health education and tighter regulation of antibiotics.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Genetics & Heredity
Yingbo Shen, Rong Zhang, Dongyan Shao, Lu Yang, Jiayue Lu, Congcong Liu, Xueyang Wang, Junyao Jiang, Boxuan Wang, Congming Wu, Julian Parkhill, Yang Wang, Timothy R. Walsh, George F. Gao, Zhangqi Shen
Summary: The emergence of mcr-1, a colistin resistance gene, has gained global attention. While the prevalence of mcr-1-positive Escherichia coli (MCRPEC) strains in human carriage has decreased between 2016 and 2019, the genetic differences in these strains remain largely unknown. Through a comparative genomic study, we found three major differences in MCRPEC strains between the two time points. These differences include changes in plasmid types, emergence of additional antibiotic resistance genes, and an increased proportion of virulence genes, indicating a potential public health threat.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Infectious Diseases
Elsa de la Cadena, Mateo Mahecha, Ana Maria Velandia, Juan Carlos Garcia-Betancur, Laura J. J. Rojas, Jessica Porras, Christian Pallares, Maria Virginia Villegas
Summary: We discovered the presence of the mcr-1 gene in a low percentage of colistin-resistant Escherichia coli isolates from Colombian hospitals. Whole genome sequencing revealed the presence of multiple resistance genes and different sequence types in the five isolates carrying mcr-1. The spread of mcr-1 in E. coli in this geographic region seems to be limited and has not affected high-risk clones like ST131.
Article
Infectious Diseases
Wenjuan Yan, Qi Zhang, Yingjie Zhu, Nan Jing, Youhua Yuan, Yi Zhang, Siying Ren, Dongmei Hu, Wenmin Zhao, Xiaojuan Zhang, Caiqin Shi, Meiyun Wang, Yi Li
Summary: The study evaluated the prevalence and characteristics of polymyxin-resistant Klebsiella pneumoniae and Escherichia coli in Henan province, China. It was found that the polymyxin resistance rate was slightly higher in K. pneumoniae compared to E. coli, and mcr-1 was more commonly detected in E. coli than in K. pneumoniae. Additionally, the insertion of ISkpn14 into mgrB may play a key role in polymyxin resistance in K. pneumoniae in Henan.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Immunology
Qiaoling Li, Changrui Qian, Xueya Zhang, Tingting Zhu, Weina Shi, Mengdi Gao, Chunlin Feng, Ming Xu, Hailong Lin, Li Lin, Junwan Lu, Xi Lin, Kewei Li, Teng Xu, Qiyu Bao, Changchong Li, Hailin Zhang
Summary: This study investigated the colistin resistance mechanism of clinical E. coli isolates using the agar dilution method, polymerase chain reaction, and comparative genomic analysis. The results showed an increase in colistin resistance in E. coli isolates in 2019 compared to previous years. The study also identified six isolates carrying the mcr-1 gene, including a novel variant with a mutation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Environmental Sciences
William Calero-Caceres, Daniel Tadesse, Katherine Jaramillo, Xavier Villavicencio, Efrain Mero, Liliana Lalaleo, Caitlin Welsh, Jose E. Villacis, Emmanuelle Quentin, Henry Parra, Maria Soledad Ramirez, Anthony D. Harries, Jose L. Balcazar
Summary: Although human activities contribute to antibiotic resistance in aquatic environments, limited information is available on the impact of discharges from countries with inadequate sewage treatment systems. This study found multidrug-resistant Escherichia coli isolates in Ecuadorian environmental samples, with some isolates carrying the mcr-1 gene along with other resistance genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Food Science & Technology
Wei Li, Yanfei Yan, Jia Chen, Ruiwen Sun, Yuxuan Wang, Tingfen Wang, Zitian Feng, Kai Peng, Juan Wang, Sheng Chen, Yanping Luo, Ruichao Li, Baowei Yang
Summary: In China, mcr-1 gene was found in both foodborne and clinical strains of Escherichia coli and Salmonella, indicating successful transfer of colistin resistance between the two bacteria. Different types of plasmids (IncI2, IncX4, and IncHI2) were identified carrying the mcr-1 gene, suggesting horizontal transfer of mcr-1 gene cassette through conjugation between wild-type bacteria. Furthermore, a new mcr-1 gene cassette was discovered, providing evidence for co-transfer of mcr-1 with other antibiotic resistance genes.
Article
Microbiology
Xiaoyu Lu, Wenhui Zhang, Mashkoor Mohsin, Mianzhi Wang, Jingui Li, Zhiqiang Wang, Ruichao Li
Summary: The prevalence and molecular characterization of mcr-1-positive isolates in pigeons close to humans were investigated after the ban on the use of colistin as an animal feed additive in China. It was found that mcr-1-positive E. coli isolates from pigeons were mainly mediated by IncX4 plasmids. However, different transferability and fitness of mcr-1-bearing IncX4 plasmids in E. coli were observed, suggesting the influence of chromosomal factors in the same bacterial species on plasmid transferability.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Valeria Shapovalova, Elvira Shaidullina, Ilya Azizov, Eugene Sheck, Alexey Martinovich, Marina Dyachkova, Alina Matsvay, Yulia Savochkina, Kamil Khafizov, Roman Kozlov, German Shipulin, Mikhail Edelstein
Summary: This study assessed the prevalence of colistin resistance and mcr genes in clinical Escherichia coli and Klebsiella pneumoniae isolates in Russia. It found a dissemination of typical mcr-bearing plasmids among diverse genotypes across a wide geographic area. The frequent association of mcr with other resistance determinants highlights the need for continual surveillance of this threat.
Article
Infectious Diseases
Yu-Chi Lin, Makoto Kuroda, Satowa Suzuki, Jung-Jung Mu
Summary: This study sequenced and analyzed the genome composition of four mcr-1-carrying plasmids isolated from Klebsiella pneumoniae clinical isolates. It was found that the dissemination of the colistin resistant gene mcr-1 in Taiwan could have been driven by various plasmids and mobile gene cassettes. The findings highlight the urgent need for continued surveillance of the worldwide distribution of mcr-1 and evaluation of the public-health risk of colistin resistance.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Public, Environmental & Occupational Health
Jialiang Mai, Zhuwei Liang, Zhile Xiong, Chao Zhang, Hao Cai, Shuwen Yao, Xiantang Chen, Bingshao Liang, Fei Gao, Zhuohong Huang, Fangjun Lan, Shuyan Liu, Zhenwen Zhou
Summary: The study investigated the sequence type and prevalence of Escherichia coli (E. coli) harboring the mcr-1 gene in the gut flora of children in southern China. The results showed that the mcr-1 gene can be horizontally transmitted within species, highlighting the importance of monitoring bacteria that carry the mcr-1 gene in children.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Microbiology
Gordan Kompes, Sanja Duvnjak, Irena Reil, Rene S. Hendriksen, Lauge Holm Sorensen, Maja Zdelar-Tuk, Boris Habrun, Luka Cvetnic, Antonela Bagaric, Silvio Spicic
Summary: In 2021, a multi-drug resistant E. coli strain with the mcr-1 gene was isolated from pig in Croatia. The isolate showed resistance to multiple antimicrobial classes and harbored various resistance genes and virulence genes. The mcr-1 gene was located within a conjugative plasmid, highlighting the importance of identifying mcr-1-carrying E. coli isolates in Croatia, especially in food-producing animals.
Article
Public, Environmental & Occupational Health
Mohammad H. Gharaibeh, Dania A. Alyafawi, Ziad A. Elnasser, Shawkat Q. Lafi, Haneen M. Obeidat
Summary: This study investigated the antimicrobial resistance profile of clinical isolates of Klebsiella pneumoniae in Jordan and found the presence of the MCR-1 gene for the first time. It also identified KPC and IMP encoded carbapenemases as the most prevalent in Jordanian patients.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Biotechnology & Applied Microbiology
Zhien He, Yongqiang Yang, Wei Li, Xiaoling Ma, Changfeng Zhang, Jingxiang Zhang, Baolin Sun, Tao Ding, Guo-Bao Tian
Summary: This study provides important information on Enterobacteriaceae bacteria carrying mcr-like genes and serves as a reference for the study of mcr-1 transmission in China and globally.
Article
Ecology
Zhuoxing Wu, Xiujuan Cai, Xin Zhang, Yao Liu, Guo-bao Tian, Jian-Rong Yang, Xiaoshu Chen
Summary: This study found that the phenotypic effects of genetic mutations are related to gene expression levels, with at least 42% of single-nucleotide mutations showing fitness effects dependent on gene expression levels and influenced by stochastic molecular errors.
NATURE ECOLOGY & EVOLUTION
(2022)
Review
Urology & Nephrology
Michael Kwok, Stephen McGeorge, Johanna Mayer-Coverdale, Bianca Graves, David L. Paterson, Patrick N. A. Harris, Rachel Esler, Caroline Dowling, Sumudu Britton, Matthew J. Roberts
Summary: This study compares recurrent urinary tract infection guidelines from major urological and non-urological organizations internationally. The results show that there is consistency in the definition and evaluation of rUTIs, as well as antibiotic prophylaxis strategies. However, there are variations in symptomatic treatment and antibiotic-sparing preventative strategies.
Article
Microbiology
Kevin B. Laupland, David L. Paterson, Felicity Edwards, Adam G. Stewart, Patrick N. A. Harris
Summary: The incidence of Morganella morganii bloodstream infections is increasing in Queensland, Australia, with elderly male subjects and individuals with comorbidities at the highest risk. Although antibiotic resistance is not a major contributor to the current burden, ongoing surveillance is necessary.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Minyon L. Avent, Kate L. McCarthy, Fekade B. Sime, Saiyuri Naicker, Aaron J. Heffernan, Steven C. Wallis, David L. Paterson, Jason A. Roberts
Summary: The role of combination antibiotic therapy for Pseudomonas aeruginosa infections is still debated. This study found that combination therapy with amikacin and meropenem increased bacterial killing for susceptible isolates, while only the combination therapy demonstrated killing for meropenem-resistant isolates.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jieying Pu, Shebin Zhang, Xi He, Jianming Zeng, Cong Shen, Yanfen Luo, Honglin Li, Yifei Long, Jianping Liu, Qian Xiao, Yang Lu, Bin Huang, Cha Chen
Summary: A novel QS regulatory sRNA AmiL has been identified in P. aeruginosa, which influences diverse QS-mediated virulence factors and regulates QS gene expression and C4-HSL production.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
David L. Paterson, Matteo Bassetti, Mary Motyl, Matthew G. Johnson, Mariana Castanheira, Erin H. Jensen, Jennifer A. Huntington, Brian Yu, Dominik J. Wolf, Christopher J. Bruno
Summary: This study confirms the efficacy of ceftolozane/tazobactam in treating HABP/VABP involving ESBL-positive and/or AmpC-producing pathogens.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Abdulrahman K. S. Ayfan, Joanne Macdonald, Adam D. Irwin, Hosam M. Zowawi, Brian M. Forde, David L. Paterson, Monica M. Lahra, David M. Whiley
Summary: This study developed instrument-free point-of-care methods using RPA technology and a simple lateral flow detection system to detect Neisseria gonorrhoeae and susceptibility to ciprofloxacin. The results showed that the developed assays were specific to N. gonorrhoeae and could effectively detect infections and antibiotic susceptibility.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Letter
Infectious Diseases
Jiachen Li, Siyuan Feng, Yuhui Chen, Meiling Yu, Wenjing Wei, Guo-Bao Tian
Article
Infectious Diseases
Burcu Isler, Abdullah Tarik Aslan, Burhan Sami Benli, David L. Paterson, Nick Daneman, Robert Fowler, Murat Akova
Summary: This study investigated the current practice of antibiotic treatment duration and timing of IV to oral switching for common bacteraemic conditions among Turkish infectious disease and ICU physicians. The results showed that the most commonly recommended duration of antibiotic treatment for bacteraemia was 14 days, followed by 10 and 7 days. Most physicians recommended switching to oral antibiotics after 7 days of IV treatment. Prolonged treatment durations may be due to a presumption that resistant bacterial infections require longer therapy.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Budi Permana, Patrick N. A. Harris, Naomi Runnegar, Margaret Lindsay, Belinda C. Henderson, E. G. Playford, David L. Paterson, Scott A. Beatson, Brian M. Forde
Summary: An outbreak of vanB-type VREfm ST78 occurred in a large tertiary Australian hospital. Whole-genome sequencing revealed a complex transmission route involving unknown environmental reservoirs. This genomic analysis provided detailed information on the local epidemiology of the outbreak strain and facilitated better targeted control measures for VREfm.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Biomedical
Zihan Zhao, Shu'an Wen, Ningning Song, Lixiang Wang, Yuan Zhou, Xue Deng, Changbu Wu, Guili Zhang, Jun Chen, Guo-Bao Tian, Minmin Liang, Lan-Lan Zhong
Summary: This study demonstrates that combining antimicrobial nanozymes with L-arginine can effectively treat difficult-to-treat Gram-negative pathogen infections. The combination treatment enhances bacterial membrane permeability and promotes ROS production, leading to the killing of pathogens.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Microbiology
Cong Shen, Jinxiang Zeng, Dexiang Zheng, Yinglun Xiao, Jieying Pu, Li Luo, Hongyun Zhou, Yimei Cai, Liling Zhang, Meina Wu, Xuan Zhang, Guangyuan Deng, Song Li, Qiwei Li, Jianming Zeng, Zhaohui Sun, Bin Huang, Cha Chen
Summary: Pseudomonas aeruginosa (P. aeruginosa) is a leading cause of chronic infections, and relapse infections are more common in carbapenem-resistant strains. This study identified relapse infections in a tertiary hospital in China and found that they were not caused by specific lineages. Genomic changes, including antimicrobial resistance and gene mutations, were observed during relapse episodes. A specific mutation in the fptA gene could be a potential target for the diagnosis and treatment of relapse P. aeruginosa infection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Xin Wen, Cong Shen, Jinyu Xia, Lan-Lan Zhong, Zhongwen Wu, Mohamed Abd El-Gawad El-Sayed Ahmed, Nana Long, Furong Ma, Guili Zhang, Wenwei Wu, Jianlve Luo, Yong Xia, Min Dai, Liyan Zhang, Kang Liao, Siyuan Feng, Cha Chen, Yishen Chen, Wenji Luo, Guo-Bao Tian
Summary: This study presents a genomic and epidemiological analysis of Clostridioides difficile in a large teaching hospital, revealing a high detection rate, diverse genome characteristics, high antibiotic resistance, and potential nosocomial transmission risk. The findings provide new insights into surveillance and prevention of C. difficile-related diseases in China.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Ama Ranasinghe, Andrew Henderson, Kyra Cottrell, Cindy S. E. Tan, Delaney Burnard, Hideo Kato, David L. Paterson, Patrick N. A. Harris
Summary: Tebipenem's in vitro activity is not affected by the production of ESBL and AmpC enzymes, and it retains its activity against strains resistant to ciprofloxacin and trimethoprim/sulfamethoxazole.
JAC-ANTIMICROBIAL RESISTANCE
(2022)