Article
Immunology
Lydia Riepler, Annika Roessler, Albert Falch, Andre Volland, Wegene Borena, Dorothee von Laer, Janine Kimpel
Summary: Neutralizing antibodies are crucial for protection against viruses like SARS-CoV-2. This study compared four different assays for detecting these antibodies and found that they were all robust and yielded comparable results, indicating their reliability.
Article
Immunology
Gyu-Lee Kim, Seong Wook Pyo, Hwajung Yi, So-Hyeon Kim, Hwachul Shin, Min-Ah Yu, Yi-Rang Hwang, Sang-Yoon Choi, Jun Ho Jeon, Su Kyoung Jo, Gi-eun Rhie
Summary: A recombinant protective antigen anthrax vaccine (GC1109) is being developed as a new-generation vaccine. The booster dose of GC1109 was found to significantly increase the production of anti-protective antigen IgG and toxin-neutralizing antibody in mice. However, the booster dose did not enhance the protective effect, as the TNA titers of the non-booster group were already high enough to provide protection against spore challenge. The study also determined the threshold TNA titer levels associated with protection.
Article
Multidisciplinary Sciences
Victoria V. Firstova, Anastasia S. Shakhova, Alena K. Riabko, Marina Silkina, Natalia A. Zeninskaya, Yana O. Romanenko, Maksim A. Marin, Methun M. Rogozin, Alena S. Kartseva, Ivan A. Dyatlov, Igor G. Shemyakin
Summary: The research studied the duration of antibodies specific to spores, protective antigen (PA), and lethal factor (LF) in the blood of donors after immunization with live anthrax vaccine. Antibodies against PA-D1 were found to circulate for over 1 year, while antibodies against LF and PA-D4 appeared after 11 months. Moderate positive correlation was observed between antibody titers and toxin neutralization activity in the blood serum samples.
Article
Peripheral Vascular Disease
Lorna M. Cryan, Tsz-Ming Tsang, Jessica Stiles, Lauren Bazinet, Sai Lun Lee, Samuel Garrard, Erika Madrian, Cody Roberts, Jessie Payne, Andrew Jensen, Arthur E. Frankel, P. Christine Ackroyd, Kenneth A. Christensen, Michael S. Rogers
Summary: Anthrax protective antigen (PA) inhibits pathological angiogenesis by interacting with capillary morphogenesis gene 2 (CMG2). Inhibiting CMG2, but not TEM8, reduces growth-factor-induced angiogenesis, highlighting CMG2 as a therapeutic target for inhibiting angiogenesis.
Review
Chemistry, Analytical
Miroslav Pohanka
Summary: An immunosensor is a biosensor used for detecting hazardous substances like aflatoxin, botulinum toxin, ricin, and Shiga toxin. It has various applications including outdoor assays, point-of-care tests, and laboratory use. Some immunosensors have been successfully commercialized as tools for toxin assays, and new devices and advanced materials and techniques are being developed to enhance their competitiveness in this field. This review summarizes current applications and new trends of immunosensors based on recent papers in this area.
Article
Microbiology
Kenneth Smith, Lori Garman, Kathleen Norris, Jennifer Muther, Angie Duke, Renata J. M. Engler, Michael R. Nelson, Limone C. Collins, Christina Spooner, Carla Guthridge, Judith A. James
Summary: Research has found that some individuals vaccinated with anthrax vaccine may produce antibodies with poor neutralizing capabilities, potentially due to increased IgG4 to IgG1 ratios, low antibody avidity, and insufficient antibody targeting specific regions. Future vaccine formulations and vaccination schedules should aim to improve these deficiencies.
Article
Biochemical Research Methods
F. Hoelzgen, R. Zalk, R. Alcalay, S. Cohen-Schwartz, G. Garau, A. Shahar, O. Mazor, G. A. Frank
Summary: Anthrax infection is associated with severe illness and high mortality, with PA being a central component of the toxin. PA plays a crucial role in anthrax progression and is an important target for monoclonal antibody-based therapy. The cAb29 antibody neutralizes the toxin by preventing pore formation through interaction with the membrane-penetrating loop of PA.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Infectious Diseases
Norman L. Mukarati, Okechukwu C. Ndumnego, Sunday O. Ochai, Solomon Jauro, Andrew Loveridge, Henriette van Heerden, Gift Matope, Alexandre Caron, Tapiwa G. Hanyire, Michel de Garine-Wichatitsky, Davies M. Pfukenyi
Summary: This study investigated the presence of anthrax antibodies in African lions in Zimbabwe, finding that approximately 21.9% of lions tested positive for anthrax antibodies. The results suggest that anthrax may be more widespread in Zimbabwe than previously realized, present in both high- and low-risk zones, including areas where outbreaks have not been reported for over 20 years. The study highlights the need for improved surveillance of anthrax in both wild and domestic animals in order to better understand and control the disease.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Microbiology
Juan Zhou, Xue-Jian Du, Ying Liu, Zeng-Qiang Gao, Zhi Geng, Yu-Hui Dong, Heng Zhang
Summary: ParE(SO)-CopA(SO) is a new type II toxin-antitoxin system that plays a crucial role in maintaining circular CP4So in Shewanella oneidensis. The CopA(SO) protein folds and binds to a positively charged and hydrophobic groove of ParE(SO) to neutralize its toxicity. ParE(SO) acts as a de-repressor of the TA operon transcription at a 1:1 toxin:antitoxin level.
Review
Food Science & Technology
Numa Piot, F. Gisou van der Goot, Oksana A. Sergeeva
Summary: AB toxins are bacterial two-component systems that utilize protein-based mechanisms to deliver toxins, providing insights into the development of bio-based drug delivery systems. With their intrinsic properties and modular characteristics, these toxins have great potential in therapy, allowing for the development of new therapeutic strategies and serving as promising tools in drug and drug delivery system development.
Article
Virology
Kimberly J. Paiva, Ricky D. Grisson, Philip A. Chan, Richard C. Huard, Angela M. Caliendo, John R. Lonks, Ewa King, Eric W. Tang, Diane L. Pytel-Parenteau, Ga H. Nam, Evgeny Yakirevich, Shaolei Lu
Summary: This study assessed the sensitivity and specificity of different serology testing methods in COVID-19 patient samples, demonstrating that SQ IgG, Abbott IgG, and Wondfo Total could detect antibodies from 100% of the PCR-confirmed patients after 14 days post-symptom onset with high concordance.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Joe James, Shelley Rhodes, Craig S. Ross, Paul Skinner, Samuel P. Smith, Rebecca Shipley, Caroline J. Warren, Hooman Goharriz, Lorraine M. McElhinney, Nigel Temperton, Edward Wright, Anthony R. Fooks, Tristan W. Clark, Sharon M. Brookes, Ian H. Brown, Ashley C. Banyard
Summary: The study analyzed serological antibody responses to SARS-CoV-2 infection during acute and convalescent stages using various detection methods. The virus neutralization test (VNT) and SARS-CoV-2-N ELISA showed strong correlation and increased detection rates when used together. The pVNT proved to be a sensitive, specific, and reproducible alternative to VNT, effectively discriminating SARS-CoV-2 antibody positive and negative serum samples across multiple institutions.
Article
Immunology
Sunday O. Ochai, Jan E. Crafford, Ayesha Hassim, Charles Byaruhanga, Yen-Hua Huang, Axel Hartmann, Edgar H. Dekker, O. Louis van Schalkwyk, Pauline L. Kamath, Wendy C. Turner, Henriette van Heerden
Summary: There are differences in exposure and immune response to anthrax among host species in different parks, which may be attributed to environmental differences and host behavior.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Xizhong Cui, Jeffrey Wang, Yan Li, Zoe G. Couse, Thomas F. Risoleo, Mahtab Moayeri, Stephen H. Leppla, Daniela Malide, Zu-Xi Yu, Peter Q. Eichacker
Summary: This study shows that Bacillus anthracis edema toxin can inhibit hypoxic pulmonary vasoconstriction, potentially worsening hypoxemia during invasive anthrax lung infection. The findings suggest that both edema toxin and lethal toxin can contribute to pulmonary pathophysiology during infection, highlighting the importance of antitoxin therapies in managing severe anthrax disease.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Microbiology
Beomki Lee, Jae-Hoon Ko, Kyoung Hwa Lee, Yong Chan Kim, Young Goo Song, Yoon Soo Park, Yae Jee Baek, Jin Young Ahn, Jun Yong Choi, Kyoung-Ho Song, Eu Suk Kim, Seongman Bae, Sung-Han Kim, Hye Won Jeong, Shin-Woo Kim, Ki Tae Kwon, Su-Hwan Kim, Hyeonji Jeong, Byoungguk Kim, Sung Soon Kim, Won Suk Choi, Kyong Ran Peck, Eun-Suk Kang
Summary: In this study, the performance of sVNT assays and binding assays in predicting neutralizing activity in vaccinees was evaluated. The results suggest that these assays can predict the protective effect by adjusting the cutoffs.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Medicinal
Julie Charton, Marion Gauriot, Jane Totobenazara, Nathalie Hennuyer, Julie Dumont, Damien Bosc, Xavier Marechal, Jamal Elbakali, Adrien Herledan, Xiaoan Wen, Cyril Ronco, Helene Gras-Masse, Antoine Heninot, Virginie Pottiez, Valerie Landry, Bart Staels, Wenguang G. Liang, Florence Leroux, Wei-Jen Tang, Benoit Deprez, Rebecca Deprez-Poulain
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Wenguang G. Liang, Min Ren, Fan Zhao, Wei-Jen Tang
JOURNAL OF MOLECULAR BIOLOGY
(2015)
Article
Multidisciplinary Sciences
Rebecca Deprez-Poulain, Nathalie Hennuyer, Damien Bosc, Wenguang G. Liang, Emmanuelle Enee, Xavier Marechal, Julie Charton, Jane Totobenazara, Gonzague Berte, Jouda Jahklal, Tristan Verdelet, Julie Dumont, Sandrine Dassonneville, Eloise Woitrain, Marion Gauriot, Charlotte Paquet, Isabelle Duplan, Paul Hermant, Francois-Xavier Cantrelle, Emmanuel Sevin, Maxime Culot, Valerie Landry, Adrien Herledan, Catherine Piveteau, Guy Lippens, Florence Leroux, Wei-Jen Tang, Peter van Endert, Bart Staels, Benoit Deprez
NATURE COMMUNICATIONS
(2015)
Article
Food Science & Technology
Carolin Luebker, Stefan Dove, Wei-Jen Tang, Ramona J. Bieber Urbauer, Jackob Moskovitz, Jeffrey L. Urbauer, Roland Seifert
Review
Endocrinology & Metabolism
Wei-Jen Tang
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2016)
Article
Biochemistry & Molecular Biology
Letif Mones, Wei-Jen Tang, Jan Florian
Article
Developmental Biology
Miho Tanaka-Matakatsu, John Miller, Daniel Borger, Wei-Jen Tang, Wei Du
DEVELOPMENTAL BIOLOGY
(2014)
Article
Chemistry, Medicinal
Julie Charton, Marion Gauriot, Qing Guo, Nathalie Hennuyer, Xavier Marechal, Julie Dumont, Malika Hamdane, Virginie Pottiez, Valerie Landry, Olivier Sperandio, Marion Flipo, Luc Buee, Bart Staels, Florence Leroux, Wei-Jen Tang, Benoit Deprez, Rebecca Deprez-Poulain
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Juan Pablo Maianti, Amanda McFedries, Zachariah H. Foda, Ralph E. Kleiner, Xiu Quan Du, Malcolm A. Leissring, Wei-Jen Tang, Maureen J. Charron, Markus A. Seeliger, Alan Saghatelian, David R. Liu
Article
Multidisciplinary Sciences
Kerstin Y. Beste, Corinna M. Spangler, Heike Burhenne, Karl-Wilhelm Koch, Yuequan Shen, Wei-Jen Tang, Volkhard Kaever, Roland Seifert
Article
Multidisciplinary Sciences
Lauren A. McCord, Wenguang G. Liang, Evan Dowdell, Vasilios Kalas, Robert J. Hoey, Akiko Koide, Shohei Koide, Wei-Jen Tang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Biochemistry & Molecular Biology
John V. King, Wenguang G. Liang, Kathryn P. Scherpelz, Alexander B. Schilling, Stephen C. Meredith, Wei-Jen Tang
Article
Biochemistry & Molecular Biology
Mara Farcasanu, Andrew G. Wang, Tomasz Uchanski, Lucas J. Bailey, Jiping Yue, Zhaochun Chen, Xiaoyang Wu, Anthony Kossiakoff, Wei-Jen Tang
Article
Chemistry, Medicinal
Nan-Sheng Li, Wenguang Liang, Joseph A. Piccirilli, Wei-Jen Tang
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Jordan M. Mancl, Cristian Suarez, Wenguang G. Liang, David R. Kovar, Wei-Jen Tang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)