Article
Nanoscience & Nanotechnology
Sampa Sarkar, Nhiem Tran, Sarvesh Kumar Soni, Zeyad Nasa, Calum J. Drummond, Charlotte E. Conn
Summary: This study successfully encapsulated and transfected siRNA into CHO-GFP cells using cationic cubosomes, demonstrating their potential as a nonviral, controlled-release delivery vector for siRNA. The gene knockdown efficiency varied depending on the type of cationic lipid used, but all cubosomes maintained the same internal nanostructure.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Maria Joao Sebastiao, Michael Hoffman, Jose Escandell, Fatemeh Tousi, Jin Zhang, Bruno Figueroa, Christine DeMaria, Patricia Gomes-Alves
Summary: Monoclonal antibody-based therapy has proven effective against various diseases, but the co-production of mispaired species can reduce its efficacy and increase production costs. This study used transcriptomics and proteomics analyses to identify signaling pathways associated with low and high mispairing clone signatures. The results showed that high mispairing clones exhibited activated endoplasmic reticulum stress, while low mispairing clones had profiles indicative of activated protein translation. Additionally, a group of genes was identified as potential biomarkers for early detection of high mispairing levels.
Review
Cell Biology
Mathilde Coulet, Oliver Kepp, Guido Kroemer, Stephane Basmaciogullari
Summary: Biotherapeutics, especially monoclonal antibodies, have become powerful tools for the treatment of various diseases. The pharmaceutical industry has made great progress in developing bioproduction processes, but understanding cellular responses to environmental changes is crucial for improving productivity. Metabolomics offers a promising approach to unlock the full potential of cellular production.
Article
Chemistry, Multidisciplinary
Eivina Radzeviciute, Arunas Murauskas, Paulius Ruzgys, Saulius Satkauskas, Irute Girkontaite, Jurij Novickij, Vitalij Novickij
Summary: In this study, a dielectrophoresis-assisted methodology for electrotransfer of plasmid DNA into mammalian cells was developed, resulting in a significant increase in transfection efficiency compared to traditional electroporation-only procedures. The use of a prototype electroporation cuvette with a center needle electrode for DEP-assisted transfection showed a 1.4-fold improvement in efficiency. The effects of different DEP frequencies and exposure times on transfection efficiency were investigated, highlighting the potential of this method as an alternative to current electrotransfection techniques.
APPLIED SCIENCES-BASEL
(2021)
Review
Food Science & Technology
Alex-Mikael Barkoff, Aapo Knuutila, Jussi Mertsola, Qiushui He
Summary: The pertussis toxin (PT) is a key virulence factor for whooping cough, with the human immune system producing a good response but serum anti-PT antibodies waning rapidly. Properly functioning PT neutralizing antibodies and anti-PT antibodies with adequate avidity are crucial for protection against the disease. The long-term B cell memory to PT is essential for lifelong immunity, but surveillance of these responses is lacking.
Article
Rheumatology
Robin Arcani, Daniel Bertin, Nathalie Bardin, Karin Mazodier, Rodolphe Jean, Pierre Suchon, Geoffroy Venton, Aurelie Daumas, Estelle Jean, Patrick Villani, Gilles Kaplanski, Pierre-Andre Jarrot
Summary: The study shows that anti-NuMA antibodies are mainly associated with pSS and SLE, and patients with these antibodies have less severe clinical profiles and require less treatment. Anti-NuMA antibodies may be a useful diagnostic marker when other auto-antibodies are negative and could indicate a good prognosis in autoimmune diseases.
Article
Biotechnology & Applied Microbiology
Matthias Noebel, Craig Barry, Michael A. Macdonald, Kym Baker, Evan Shave, Stephen Mahler, Trent Munro, Veronica S. Martinez, Lars K. Nielsen, Esteban Marcellin
Summary: Chinese Hamster Ovary (CHO) cells have been increasingly used in biopharmaceutical production, particularly in perfusion culture mode. However, most current cell lines have been optimized for fed-batch culture methods and may not perform well under perfusion conditions. In this study, a triple knockout CHO cell line deficient in three apoptosis related genes was cultured in a perfusion system to improve cell resilience and viability. After an initial growth arrest phase, the cells entered a second growth phase, although with reduced productivity. Proteomics analysis revealed a correlation between growth arrest and cell cycle arrest, as well as upregulation of central carbon metabolism and oxidative phosphorylation. This upregulation was partially reversed during the second growth phase, suggesting intragenerational adaptations to stress. Our findings shed light on metabolic regulation and the potential development of better cell lines for perfusion cultivation.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Qin Li, Rui-Fang Yan, Yong-Xiao Yang, Chun-liu Mi, Yan-long Jia, Tian-Yun Wang
Summary: This study demonstrates that SAR elements can enhance transgene expression and maintain long-term expression in CHO cells. This has significant implications for increasing recombinant protein production in CHO cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ismael Obaidi, Leticia Martins Mota, Andrew Quigley, Michael Butler
Summary: In an experiment using CHO cell lines to produce mAbs with various protein hydrolysates, it was found that the presence of hydrolysates led to increased cell growth density and mAb productivity. Different hydrolysates had varying effects on the enhancement of mAb productivity, with UPcotton hydrolysate showing the most significant improvement.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hirra Hussain, Tulshi Patel, Angelica M. S. Ozanne, Davide Vito, Mark Ellis, Matthew Hinchliffe, David P. Humphreys, Paul E. Stephens, Bernie Sweeney, James White, Alan J. Dickson, Christopher M. Smales
Summary: Monoclonal antibodies are the most approved class of biopharmaceuticals for therapeutic purposes, with Fab fragments also being widely used in research applications. While mammalian cell-based systems are dominant for full-length antibodies, Fab molecules are often expressed in bacterial systems. Advances in CHO technologies have made mammalian systems equally viable for Fab production, with CHO cells showing greater efficiency in producing Fab molecules compared to full-length antibodies. Gene copy number was not found to be correlated with protein production, suggesting the need for targeted engineering to improve production efficiency.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Analytical
Jing Lei, Lu Shi, Wei Liu, Baoxin Li, Yan Jin
Summary: A simple and sensitive chemiluminescence method using a handheld luminometer was proposed for portable detection of cancer cells. The method utilizes a cyclic strand displacement reaction for signal amplification and achieves sensitive detection of cancer cells with a low detection limit. This provides a new option for the early diagnosis of cancers.
Review
Medicine, General & Internal
Michal Braczkowski, Dariusz Soszynski, Alicja Sierakowska, Ryszard Braczkowski, Klaudia Kufel, Beata Labuz-Roszak
Summary: Encephalitis is a complex condition with various causes and symptoms. Autoimmune encephalitis, which accounts for about 4% of encephalitis cases, is caused by autoantibodies attacking the central nervous system. This paper discusses the mental and neurological symptoms of autoimmune encephalitis, as well as the coexistence with psychosis and neoplastic diseases. Early diagnosis and targeted treatment are emphasized for a successful therapeutic process. The research methodology involved searching for articles from 2007 to 2023 using search engines like PubMed and Medline.
Article
Immunology
Manuel Saez Moya, Rebeca Gutierrez-Cozar, Joan Punet-Ortiz, Maria Luisa Rodriguez de la Concepcion, Julia Blanco, Jorge Carrillo, Pablo Engel
Summary: In a study using NOD.H-2 (h4) mice as a model for spontaneous SjS, it was found that these mice have a higher frequency of self-reactive B-cell clones, and with age, there is an increase in polyreactive and autoreactive IgG clones. Furthermore, in these mice, there is a trend towards oligoclonality in IgG B cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Natalia Madetko, Weronika Marzec, Agata Kowalska, Dominika Przewodowska, Piotr Alster, Dariusz Koziorowski
Summary: This article summarizes the current knowledge on anti-IgLON5 disease, including its etiology, symptomatology, clinical progression, differential diagnosis, and treatment. The disease presents a challenge due to its nonspecific symptoms, diverse clinical course, and complex nature.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Mungyo Jung, Songmin Lee, Sohui Park, Jihye Hong, Cheesue Kim, Illhwan Cho, Hee Su Sohn, Kyunghwan Kim, In Wook Park, Soljee Yoon, Sungpil Kwon, Jisu Shin, Donghee Lee, Mikyung Kang, Seokhyung Go, Sangjun Moon, Yeonseok Chung, YoungSoo Kim, Byung-Soo Kim
Summary: Alzheimer's disease, the most common cause of dementia, has multiple pathophysiological mechanisms. Current immunotherapy approaches targeting single mechanism have limitations. A nanovaccine has been developed to target multiple mechanisms and show higher efficacy in treating Alzheimer's disease in mice. The nanovaccine is easy-to-prepare and cost-effective, providing a novel treatment option for Alzheimer's disease.
ADVANCED MATERIALS
(2023)
Article
Peripheral Vascular Disease
Suellen C. Coelho, Olga Berillo, Antoine Caillon, Sofiane Ouerd, Julio C. Fraulob-Aquino, Tlili Barhoumi, Stefan Offermanns, Pierre Paradis, Ernesto L. Schiffrin
Article
Multidisciplinary Sciences
Sorin Tunaru, Remy Bonnavion, Isabell Brandenburger, Jens Preussner, Dominique Thomas, Klaus Scholich, Stefan Offermanns
NATURE COMMUNICATIONS
(2018)
Article
Immunology
Julian Albarran-Juarez, Andras Iring, ShengPeng Wang, Sayali Joseph, Myriam Grimm, Boris Strilic, Nina Wettschureck, Till F. Althoff, Stefan Offermanns
JOURNAL OF EXPERIMENTAL MEDICINE
(2018)
Article
Developmental Biology
Christian S. M. Helker, Sri-Teja Mullapudi, Laura M. Mueller, Jens Preussner, Sorin Tunaru, Oskar Skog, Hyouk-Bum Kwon, Florian Kreuder, Joseph J. Lancman, Remy Bonnavion, P. Duc Si Dong, Mario Looso, Stefan Offermanns, Ole Korsgren, Francesca M. Spagnoli, Didier Y. R. Stainier
Article
Immunology
Jason D. Simmons, Glenna J. Peterson, Monica Campo, Jenny Lohmiller, Shawn J. Skerrett, Sorin Tunaru, Stefan Offermanns, David R. Sherman, Thomas R. Hawn
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Cardiac & Cardiovascular Systems
Guozheng Liang, ShengPeng Wang, Jingchen Shao, Young-June Jin, Liran Xu, Yang Yan, Stefan Guenther, Lei Wang, Stefan Offermanns
Summary: This study reveals that TN-X is a central mediator of flow-induced inhibition of EndMT, endothelial inflammation, and atherogenesis by binding to and blocking the activity of TGF-beta. The research uncovers a novel mechanism of flow-dependent regulation of vascular TGF-beta, which holds promise for generating new strategies to prevent vascular inflammation and atherosclerosis.
CIRCULATION RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Nils Helge Schebb, Hartmut Kuehn, Astrid S. Kahnt, Katharina M. Rund, Valerie B. O'Donnell, Nicolas Flamand, Marc Peters-Golden, Per-Johan Jakobsson, Karsten H. Weylandt, Nadine Rohwer, Robert C. Murphy, Gerd Geisslinger, Garret A. FitzGerald, Julien Hanson, Claes Dahlgren, Mohamad Wessam Alnouri, Stefan Offermanns, Dieter Steinhilber
Summary: This paper critically evaluates the biosynthetic pathways of specialized pro-resolving lipid mediators (SPMs), their receptors and signaling cascades, and the analytical methods used to quantify these mediators. It concludes that there is limited biosynthetic capacity for SPMs in human leukocytes, the identity and signaling of proposed SPM receptors need further validation, SPM levels are not related to dietary supplementation or resolution of inflammation, and the commonly reported methodology for quantification of SPMs is unreliable.
FRONTIERS IN PHARMACOLOGY
(2022)
Editorial Material
Hematology
Brendan J. O'Brien, Kathleen A. Martin, Stefan Offermanns
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Saskia Wedel, Lisa Hahnefeld, Mohamad Wessam Alnouri, Stefan Offermanns, Felix Hausch, Gerd Geisslinger, Marco Sisignano
Summary: Neuropathic pain is a common condition with limited treatment options. This study reveals that the FKBP51 inhibitor SAFit2 restores lipid signaling and metabolism in nervous tissue, reducing inflammation and pain sensitivity, thus providing analgesic properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Akiko Nakayama, Kenneth Anthony Roquid, Andras Iring, Boris Strilic, Stefan Guenther, Min Chen, Lee S. Weinstein, Stefan Offermanns
Summary: The authors identify a tumor-suppressing angiocrine function of CCL2 and a pro-angiogenic and tumor-promoting effect of tumor cell-derived adrenomedullin. They also show that endothelium-derived CCL2 suppresses adrenomedullin-induced tumor cell proliferation. These findings highlight the importance of the interaction between tumor cells and endothelial cells in the tumor microenvironment.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Oncology
Boris Shorning, Neil Trent, David F. Griffiths, Thomas Worzfeld, Stefan Offermanns, Matthew J. Smalley, Magali Williamson
Summary: Metastatic prostate cancer is a fatal disease, and the mechanisms behind it are poorly understood. This study identified a mutant form of Plexin-B1 (P1597L) in metastatic deposits of prostate cancer patients, which greatly increased metastasis in transgenic mouse models. In contrast, wild-type Plexin-B1 significantly decreased metastasis. The study also revealed the involvement of the Rho/ROCK pathway in the phenotypic switch and suggested Plexin-B1 as a potential therapeutic target to block the spread of the disease.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Physiology
Helga Balla, Kinga Borsodi, Petra Orsy, Bela Horvath, Peter Jozsef Molnar, Adam Lenart, Monika Kosztelnik, Eva Ruisanchez, Juergen Wess, Stefan Offermanns, Peter Nyirady, Zoltan Benyo
Summary: Acetylcholine plays an essential role in bladder muscle contractions, and antimuscarinics are commonly used for overactive bladder syndrome. This study found that both M-2 and M-3 receptors, as well as G(i) proteins, mediate acetylcholine-induced bladder contractions and RhoA activation, suggesting potential therapeutic targets for bladder dysfunctions.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Lei Wang, ShengPeng Wang, Yue Shi, Rui Li, Stefan Guenther, Yu Ting Ong, Michael Potente, Zuyi Yuan, Enqi Liu, Stefan Offermanns
NATURE COMMUNICATIONS
(2020)
Article
Physiology
Balint Der, Peter Jozsef Molnar, Eva Ruisanchez, Petra Orsy, Margit Kerek, Bernadett Farago, Peter Nyirady, Stefan Offermanns, Zoltan Benyo
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2019)