Article
Engineering, Biomedical
Maria Chiara Munisso, Susumu Saito, Itaru Tsuge, Naoki Morimoto
Summary: Quantitative structural analysis of collagen fiber orientation is crucial for understanding connective tissue behavior. We developed a simplified method for comparing multiple human skin samples to analyze load-dependent changes using SHG microscopy. Our results showed that increased load led to more aligned fibers in the loading direction and revealed the presence of two preferred orientations in both perpendicular and parallel planes to the epidermis.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Sammy Hermans, Jacob Pilon, Dennis Eschweiler, Johannes Stegmaier, Carmen A. H. Severens-Rijvers, Salwan Al-Nasiry, Marc van Zandvoort, Dimitrios Kapsokalyvas
Summary: Pre-eclampsia is a severe complication in pregnancy with limited diagnostic and therapeutic options, and there is controversy surrounding its etiology and phenotypes. Using multiphoton microscopy (MPM) imaging, the native placental three-dimensional morphology can be visualized with subcellular resolution, allowing for the identification of various imaging targets such as trophoblast organization, villous tree structure, syncytial knots, fibrosis, and vascular networks. Preliminary data suggests that pre-eclampsia is characterized by increased syncytial knot density, paddle-like villous sprouts, abnormal villous volume-to-surface ratio, and decreased vascular density. Quantifying 3D microscopic images shows potential for phenotyping pre-eclampsia in placental villous tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yongping Lin, Hongxin Lin, Xiaoqin Zhu, Guannan Chen
Summary: A new method for quantitatively analyzing three-dimensional elastic fibers in human cutaneous scars was designed, with extracted parameters being able to predict scar duration. Random forests regression showed better performance in forecasting scar duration than unitary models.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2021)
Review
Dermatology
Ke-Jun Chen, Yang Han, Zi-Yi Wang, Yong Cui
Summary: Non-invasive optical examination is vital in dermatology for diagnosis, management, and research. Multiphoton microscopy uses submicron technology to stimulate autofluorescence and provides insights into cellular structure, redox status, and collagen fibers in the skin. This review highlights the principles and application of multiphoton microscopy in studying various skin conditions.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Immunology
Rui Mao, Fan Yang, Tongtong Zhang, Ji Li
Summary: The incidence of cutaneous melanoma is rising, and better markers are needed to predict prognosis and immunotherapy effectiveness. By analyzing TCGA data and developing a signature based on immune checkpoint proteins, researchers were able to accurately predict prognosis and response to therapy in melanoma patients. The signature was found to be an independent prognostic factor and a nomogram based on the signature was more effective than the signature alone.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Sabrina Valetti, Hanna Thomsen, Jitendra Wankar, Peter Falkman, Ilse Manet, Adam Feiler, Marica B. Ericson, Johan Engblom
Summary: Research has shown that porous particles of appropriate size and porosity can accumulate in hair follicles and furrows on the skin, providing a potential area for drug delivery. The combination of follicle orifices and furrows can cover up to 20% of the skin surface, making them valuable for optimizing bioavailability of topical formulations. This work highlights the potential of skin furrows for topical drug delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Optics
Jianhua Zhao, Yuan Zhao, Zhenguo Wu, Yunxian Tian, Haishan Zeng
Summary: Multi-photon microscopy (MPM) and coherent anti-Stokes Raman scattering (CARS) are two advanced nonlinear optical imaging techniques that provide complementary information and have great potential for noninvasive in vivo biomedical applications. This paper discusses the basics, development, and applications of MPM and CARS in in vivo skin research, including their principles, instrumentation development for in vivo applications, imaging of normal skin, applications in skin cancer and disease diagnosis, and intervention in skin diseases using MPM-guided two-photon photothermolysis.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
David M. Chesko, Traci A. Wilgus
Summary: This review summarizes the roles of different immune cell types in cutaneous wound healing, with a focus on data from animal studies targeting specific cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Marco Leonetti, Lorenzo Pattelli, Simone De Panfilis, Diederik S. Wiersma, Giancarlo Ruocco
Summary: Through directly measuring spatially resolved intensity correlations of light inside a disordered medium using DNA strings decorated with emitters, researchers uncovered deviations in the size and polarization degrees of freedom of bulk speckles from theoretical predictions. These deviations are explained by correlations among polarization components and non-universal near-field contributions at the nanoscale.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Nilakshi Barua, Lin Huang, Carmen Li, Ying Yang, Mingjing Luo, Wan In Wei, Kam Tak Wong, Norman Wai Sing Lo, Kin On Kwok, Margaret Ip
Summary: In this study, a 3D organotypic keratinocyte-fibroblast co-culture model was established to evaluate the effectiveness of a 3D skin model in studying cell death induced by Methicillin-resistant Staphylococcus aureus (MRSA) compared to a 2D HaCaT monolayer. The results showed that the 3D skin model provided better insights into the physiological response of skin to MRSA infection and revealed variations in pathogenesis among different MRSA strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Anna Leikeim, Maximiliane Wussmann, Freia F. Schmidt, Nuno G. B. Neto, Franziska Benz, Kendra Tiltmann, Corinna Junger, Michael G. Monaghan, Bastian Schilling, Florian K. Groeber-Becker
Summary: Malignant melanoma is a rapidly growing tumor entity worldwide. Researchers have developed a melanoma skin equivalent model based on an open-source epidermal model, successfully incorporating melanoma cells with different driver mutations to form distinguishable tumor aggregates. This model has shown potential for tumor therapeutics, as demonstrated by successful treatment with a BRAF inhibitor vemurafenib in models harboring BRAF-mutated melanoma cells.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Xuesong Li, Yicong Wu, Yijun Su, Ivan Rey-Suarez, Claudia Matthaeus, Taylor B. B. Updegrove, Zhuang Wei, Lixia Zhang, Hideki Sasaki, Yue Li, Min Guo, John P. P. Giannini, Harshad D. D. Vishwasrao, Jiji Chen, Shih-Jong J. Lee, Lin Shao, Huafeng Liu, Kumaran S. S. Ramamurthi, Justin W. W. Taraska, Arpita Upadhyaya, Patrick La Riviere, Hari Shroff
Summary: Enhanced axial resolution for 3D SIM is achieved with deep learning or four-beam interference. These methods allow for higher spatial frequency content and near-isotropic imaging with resolutions of around 120 nm in both lateral and axial directions. The deep learning method can also be combined with denoising for volumetric imaging. Various cellular samples were imaged to demonstrate the potential of these advancements.
NATURE BIOTECHNOLOGY
(2023)
Article
Neurosciences
Michael A. Thornton, Gregory L. Futia, Michael E. Stockton, Baris N. Ozbay, Karl Kilborn, Diego Restrepo, Emily A. Gibson, Ethan G. Hughes
Summary: The use of three-photon microscopy significantly improves the depth and resolution of in vivo imaging, making it important for studying cellular interactions and dynamics in the brain. This study explored the application of tdTomato or mScarlet and EGFP for dual-color single-excitation structural 3P imaging in the living mouse brain, and found that tdTomato and mScarlet have high signal-to-background ratios in a specific wavelength range, enhancing the imaging quality.
Article
Multidisciplinary Sciences
Flavien Bureau, Justine Robin, Arthur Le Ber, William Lambert, Mathias Fink, Alexandre Aubry
Summary: Matrix imaging is revolutionizing wave physics by optimizing compensation for aberrations and multiple scattering in heterogeneous media. This paper extends ultrasound matrix imaging to a 3D geometry and proposes a non-invasive approach to make the skull transparent for improved brain imaging.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Weikai Xue, Jonas Ogien, Pavel Bulkin, Anne-Lise Coutrot, Arnaud Dubois
Summary: This study aimed to develop a more compact and lighter LC-OCT device that can acquire images faster without significant degradation of resolution. The device is based on a Mirau interferometer using a single objective and incorporates a galvanometer scanner for lateral scanning of the illumination line. The optimized device can acquire B-scans at an unprecedented rate of 17 fps and produce 3D images of human skin at cellular resolution.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Immunology
Renhua Song, Shweta Tikoo, Rohit Jain, Natalia Pinello, Amy Y. M. Au, Rajini Nagarajah, Bo Porse, John E. J. Rasko, Justin J-L Wong
Summary: Monocytes play a crucial role in immune responses, and the intron retention mechanism during their differentiation pathway is found to be essential in regulating the differentiation process, with distinct intron retention differences observed among different subsets of monocytes.
Article
Pathology
Adam O. Michel, Taryn A. Donovan, Ben Roediger, Quintin Lee, Christopher J. Jolly, Sebastien Monette
Summary: This study investigated the possible role of chaphamaparvoviruses in the development of feline chronic kidney disease, but found no evidence to suggest that viruses belonging to this genus are likely to contribute to the occurrence of CKD in cats.
VETERINARY PATHOLOGY
(2022)
Article
Biology
Manuela Ecker, Richard Schregle, Natasha Kapoor-Kaushik, Pascal Rossatti, Verena M. Betzler, Daryan Kempe, Mate Biro, Nicholas Ariotti, Gregory M. Redpath, Jeremie Rossy
Summary: This study investigates the recruitment of SNX9 to CD28 clusters upon CD28 triggering, which promotes membrane tubulation and regulates the stability of CD28 as well as signaling events. CD28 clusters are shown to be dynamic structures, and SNX9-mediated tubulation generates a membrane environment that facilitates CD28 triggering and downstream signaling events.
Review
Medicine, Research & Experimental
Natasha Kolesnikoff, Chun-Hsien Chen, Michael Susithiran Samuel
Summary: Solid tumours are composed of cancer cells with genetic mutations, but also contain genetically normal cells and extracellular matrix (ECM). The ECM plays a crucial role in tumour cell proliferation and survival, and also influences the function and access of immune cells in the tumour microenvironment.
Review
Allergy
Ben Roediger, Christoph Schlapbach
Summary: The pathogenesis of atopic dermatitis and psoriasis, as well as cutaneous T-cell lymphoma, involves the role of T cells and cytokine-mediated inflammation. Skin-resident memory T cells and cytokines produced by malignant or pathogenic T cells are crucial in driving skin inflammation and symptoms. However, current therapies mainly focus on targeting T-cell effector functions or malignant T cells, without addressing the chronicity of the diseases.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Tim Hendrikx, Florentina Porsch, Mate G. Kiss, Dragana Rajcic, Nikolina Papac-Milicevic, Constanze Hoebinger, Laura Goederle, Anastasiya Hladik, Lisa E. Shaw, Hauke Horstmann, Sylvia Knapp, Sophia Derdak, Martin Bilban, Lena Heintz, Marcin Krawczyk, Rafael Paternostro, Michael Trauner, Matthias Farlik, Dennis Wolf, Christoph J. Binder
Summary: This study investigates the role of bone marrow-derived TREM2-expressing macrophages in non-alcoholic steatohepatitis (NASH). The results show that these macrophages are involved in hepatocellular damage, inflammation, and fibrosis in the liver. Soluble TREM2 levels in the blood are identified as a potential circulating marker for NASH.
JOURNAL OF HEPATOLOGY
(2022)
Article
Cell Biology
Matt A. Govendir, Daryan Kempe, Setareh Sianti, James Cremasco, Jessica K. Mazalo, Feyza Colakoglu, Matte Golo, Kate Poole, Mate Biro
Summary: Opposing cytoskeletal forces drive lytic granule polarization and shape T cell synapse topography to enhance target perforation. This study reveals a curvature bias in the action of perforin, preferentially perforating positively curved tumor cell membrane.
DEVELOPMENTAL CELL
(2022)
Article
Rheumatology
Maximilian Kugler, Mirjam Dellinger, Felix Kartnig, Lena Mueller, Teresa Preglej, Leonhard X. Heinz, Elisabeth Simader, Lisa Goeschl, Stephan E. Puchner, Sebastian Weiss, Lisa E. Shaw, Matthias Farlik, Wolfgang Weninger, Giulio Superti-Furga, Josef S. Smolen, Guenter Steiner, Daniel Aletaha, Hans P. Kiener, Myles J. Lewis, Costantino Pitzalis, Anela Tosevska, Thomas Karonitsch, Michael Bonelli
Summary: Activated FLS play a critical role in synovial tissue remodeling and interaction with immune cells in rheumatoid arthritis (RA). Co-culture experiments demonstrated that cytokine-activated FLS enhance T cell activation, proliferation, and differentiation. Transcriptomic analysis revealed a correlation between FLS cytokine signatures and infiltrating CD4(+) T cells in RA synovial tissue. ICAM1 was identified as an important mediator in TNF-mediated FLS-T cell interaction.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Hematology
Sevgi Kostel Bal, Sarah Giuliani, Jana Block, Peter Repiscak, Christoph Hafemeister, Tala Shahin, Nurhan Kasap, Bernhard Ransmayr, Yirun Miao, Cheryl van de Wetering, Alexandra Frohne, Raul Jimenez Heredia, Michael Schuster, Samaneh Zoghi, Vanessa Hertlein, Marini Thian, Aleksandr Bykov, Royala Babayeva, Sevgi Bilgic Eltan, Elif Karakoc-Aydiner, Lisa E. Shaw, Iftekhar Chowdhury, Markku Varjosalo, Rafael J. Arguello, Matthias Farlik, Ahmet Ozen, Edgar Serfling, Loic Dupre, Christoph Bock, Florian Halbritter, J. Thomas Hannich, Irinka Castanon, Michael J. Kraakman, Safa Baris, Kaan Boztug
Summary: In this study, the researchers investigated the role of NFATC1 mutations in human immunity and found evidence of metabolic plasticity in patient T cells. The study also demonstrated that metformin and rosiglitazone can improve the effector functions of patient T cells.
Article
Biochemistry & Molecular Biology
Abigail K. Grootveld, Wunna Kyaw, Veera Panova, Angelica W. Y. Lau, Emily Ashwin, Guillaume Seuzaret, Rama Dhenni, Nayan Deger Bhattacharyya, Weng Hua Khoo, Mate Biro, Tanmay Mitra, Michael Meyer-Hermann, Patrick Bertolino, Masato Tanaka, David A. Hume, Peter I. Croucher, Robert Brink, Akira Nguyen, Oliver Bannard, Tri Giang Phan
Summary: Germinal centers (GCs) in lymphoid follicles during antibody responses are areas of extensive cell death. Tingible body macrophages (TBMs) derived from lymph node-resident, CD169-lineage, CSF1R-blockade-resistant precursor. Non-migratory TBMs use cytoplasmic processes to capture migrating dead cell fragments using a lazysearch strategy. Follicular macrophages can mature into TBMs in the absence of GCs and activated by the nearby apoptotic cells. Single-cell transcriptomics analysis identified upregulated genes related to apoptotic cell clearance in TBMs, which prevent autoimmune diseases.
Article
Allergy
Natalia Alkon, Frank P. Assen, Tamara Arnoldner, Wolfgang M. Bauer, Marco A. Medjimorec, Lisa E. Shaw, Katharina Rindler, Gregor Holzer, Philipp Weber, Wolfgang Weninger, Christian Freystatter, Sumanth Chennareddy, Tamar Kinaciyan, Matthias Farlik, Constanze Jonak, Johannes Griss, Christine Bangert, Patrick M. Brunner
Summary: This study used single-cell RNA sequencing combined with T-cell receptor sequencing to compare skin lesions from patients with chronic nodular prurigo (CNPG), atopic dermatitis (AD), and healthy individuals. The results showed that both CNPG and AD exhibited type 2 immune skewing, but only AD had an additional population of CD8A+IL9R+IL13+ cytotoxic T-cells and highly upregulated immune activation pathways. In contrast, CNPG showed characteristics of stromal remodeling and fibrosis. These findings contribute to a better understanding of the pathomechanisms and molecular relationships between CNPG and AD.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Dermatology
Katharina Rindler, Philipp Tschandl, Jasmine P. Levine, Lisa E. Shaw, Wolfgang Weninger, Matthias Farlik, Constanze Jonak, Patrick M. Brunner
Summary: This study conducted single-cell RNA sequencing analysis on a COVID-19 patient with a skin rash. The results show the expression of ACE2 and TMPRSS2, the entry receptors for SARS-CoV-2, in the patient's keratinocytes, indicating profound transcriptional changes in these cells. Inflammatory immune cells also exhibited characteristics of immune response. These findings are important for understanding SARS-CoV-2-associated skin conditions.
JOURNAL OF DERMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Irfete S. Fetahu, Wolfgang Esser-Skala, Rohit Dnyansagar, Samuel Sindelar, Fikret Rifatbegovic, Andrea Bileck, Lukas Skos, Eva Bozsaky, Daria Lazic, Lisa Shaw, Marcus Totzl, Dora Tarlungeanu, Marie Bernkopf, Magdalena Rados, Wolfgang Weninger, Eleni M. Tomazou, Christoph Bock, Christopher Gerner, Ruth Ladenstein, Matthias Farlik, Nikolaus Fortelny, Sabine Taschner-Mandl
Summary: The authors performed single cell RNA-seq and ATAC-seq of bone marrow aspirates from 16 subjects with neuroblastoma and found that tumor cell plasticity is conserved in metastasis. They also identified tumor-to-bone marrow cell signals that trigger tumor-promoting monocytes. This study provides insights into therapeutic approaches targeting tumor-to-microenvironment interactions.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Matthew B. O'Rourke, Ben R. Roediger, Christopher J. Jolly, Ben Crossett, Matthew P. Padula, Phillip M. Hansbro
Summary: In this study, a hybrid bioinformatic/image-based method was developed for the identification and validation of viral biomarkers. This method leverages the power of mass spectrometry imaging and provides confident identifications for viral proteins without requiring MS/MS.
Article
Multidisciplinary Sciences
Henry Cavanagh, Daryan Kempe, Jessica K. Mazalo, Mate Biro, Robert G. Endres
Summary: This study utilized advanced technology to explore the three-dimensional morphodynamics of T cells, revealing low-dimensional shape space and fixed migration patterns that can be broken down into specific steps.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)