Article
Otorhinolaryngology
Kosuke Terazawa, Toshimitsu Ohashi, Hirofumi Shibata, Takuma Ishihara, Takenori Ogawa
Summary: In this study, we investigated the prognostic role of immune-mGPS (imGPS) in patients with head and neck squamous cell carcinoma (HNSCC) and found that imGPS is a more accurate predictor compared to the conventional mGPS.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2022)
Article
Biochemistry & Molecular Biology
Barbora Pokryvkova, Marek Grega, Jan Klozar, Ondrej Vencalek, Jaroslav Nunva, Ruth Tachezy
Summary: This study retrospectively analyzed tissue samples from oral and oropharyngeal carcinomas associated and non-associated with HPV infection and found that HPV-positive tumors had more T lymphocyte infiltration. The number of CD8(+)PD-1(+) T cells was identified as an independent factor influencing the survival of patients with oral cavity or oropharyngeal carcinomas.
Article
Oncology
Olgun Elicin, Eliane Koller Brolese, Beat Bojaxhiu, Burim Sermaxhaj, Daniel Hendrik Schanne, Etienne Mathier, Jana Lippmann, Mohamed Shelan, Yannick Eller, Daniel Matthias Aebersold, Roland Giger, Nikola Cihoric
Summary: This study investigated the impact of seasonal variation on the prognosis of non-metastatic head and neck squamous cell carcinoma patients treated with radiotherapy. Results showed that better loco-regional control and progression-free survival were achieved when radiotherapy was administered during the darker season. However, the biological mechanism behind this phenomenon remains unclear and further external validation is needed.
RADIOTHERAPY AND ONCOLOGY
(2021)
Article
Oncology
Erik Haehl, Luisa Alvino, Alexander Ruhle, Jiadai Zou, Alexander Fabian, Anca-Ligia Grosu, Nils H. Nicolay
Summary: Sarcopenia, or the loss of muscle mass, is a risk factor for reduced survival and increased treatment-associated toxicities in many cancers, and it increases with age. In this study, we investigated sarcopenia and sarcopenia dynamics during treatment in elderly patients with head-and-neck cancers undergoing (chemo)radiotherapy. Pre-treatment sarcopenia in elderly patients correlated with decreased body weight, a higher level of comorbidities, larger tumor size, and increasing age. Additionally, patients who exhibited sarcopenia prior to radiotherapy had significantly reduced overall survival and an increased risk of higher-grade toxicities. However, during (chemo)radiotherapy, muscle mass in patients remained relatively stable, and post-therapeutic sarcopenia did not correlate with patient survival.
Review
Multidisciplinary Sciences
Chih-Wei Luan, Yao-Te Tsai, Hsin-Yi Yang, Kuan-Yin Chen, Po-Hsien Chen, Hsin-Hsu Chou
Summary: The pretreatment prognostic nutritional index (PNI) has controversial predictive value for head and neck cancer (HNC) patients. Low pretreatment PNI is associated with poor overall survival (OS), progression-free survival (PFS), disease-specific survival (DSS), disease-free survival (DFS) and distant metastasis-free survival (DMFS) in HNC patients, regardless of variations in cancer site, sample size, PNI cut-off value, analysis method and treatment modality. Elevated pretreatment PNI is correlated with a better prognosis in HNC patients and could serve as a biomarker for prognosis prediction and treatment stratification in clinical practice.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Daniele Borsetto, Jerry Polesel, Giancarlo Tirelli, Anna Menegaldo, Vittorio Baggio, Alessandro Gava, Paul Nankivell, Paul Pracy, Jonathan Fussey, Paolo Boscolo-Rizzo
Summary: This study found that pretreatment anemia and macrocytosis are independent predictors of lower overall and progression-free survival in patients with head and neck squamous cell carcinoma undergoing organ preservation treatment.
Article
Oncology
Kristian Hastoft Jensen, Ivan Vogelius, Claus Ernst Moser, Elo Andersen, Jesper Grau Eriksen, Jorgen Johansen, Mohammad Farhadi, Maria Andersen, Jens Overgaard, Jeppe Friborg
Summary: This study found that the risk of bloodstream infections (BSIs) in patients with head and neck squamous cell carcinoma undergoing radiotherapy or chemoradiation is associated with age, cancer stage, and performance status, with Staphylococcus aureus being a common pathogen. The 30-day post-BSI mortality rate was 26%, and BSIs contributed to 10% of early non-cancer deaths. Empiric antibiotic treatment should prioritize coverage for S. aureus in this population.
BRITISH JOURNAL OF CANCER
(2021)
Review
Health Care Sciences & Services
Martin Dolezel, Marek Slavik, Tomas Blazek, Tomas Kazda, Pavel Koranda, Lucia Veverkova, Petr Burkon, Jakub Cvek
Summary: Concurrent chemoradiotherapy is commonly used in the curative treatment of head and neck cancer patients, with locoregional failure being the main recurrence pattern. Tumor hypoxia is a major cause of treatment failure, and positron emission tomography (PET) has been extensively studied as a feasible method for detecting tumor hypoxia. Escalating the dose to hypoxic tumors has shown potential to improve outcomes. The optimization of radiotherapeutic plans has been well-documented, and there are two main approaches for defining the hypoxic tumor volume based on PET images.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Nutrition & Dietetics
Dong Pan, Qingyu Shen, Yi Li, Xiaoming Rong, Honghong Li, Yongteng Xu, Baixuan He, Xuzheng Zuo, Zhenhong Deng, Yamei Tang
Summary: This study investigated the relationship between nutritional risk status and overall survival in patients with radiation-induced brain necrosis (RN). The results showed that malnutrition at admission was associated with an increased risk of future death. The utilization of composite nutritional risk assessment indices upon admission could help identify high-risk patients and improve nutritional management.
Review
Medicine, General & Internal
Mayur D. Mody, James W. Rocco, Sue S. Yom, Robert Haddad, Nabil F. Saba
Summary: Head and neck cancer is the seventh most common type of cancer worldwide, with the predominant risk factors being tobacco use, alcohol abuse, and oncogenic viruses. Treatment typically involves a multidisciplinary approach with surgery, radiotherapy, and systemic therapy, and is challenging due to site-specific and histology-specific variations.
Article
Nutrition & Dietetics
Giuseppe Fanetti, Jerry Polesel, Elisabetta Fratta, Elena Muraro, Valentina Lupato, Salvatore Alfieri, Carlo Gobitti, Emilio Minatel, Fabio Matrone, Angela Caroli, Alberto Revelant, Marco Lionello, Viviana Zammattio Polentin, Andrea Ferretti, Roberto Guerrieri, Paola Chiovati, Andy Bertolin, Vittorio Giacomarra, Antonino De Paoli, Emanuela Vaccher, Giovanna Sartor, Agostino Steffan, Giovanni Franchin
Summary: The study found that PNI is an important parameter related to toxicity in head and neck cancer treatment, with low PNI levels associated with increased risk of weight loss and late toxicity. PNI plays a significant role in identifying high-risk head and neck cancer patients for acute and late toxicity.
Article
Multidisciplinary Sciences
William Trung Le, Eugene Vorontsov, Francisco Perdigon Romero, Lotfi Seddik, Mohamed Mortada Elsharief, Phuc Felix Nguyen-Tan, David Roberge, Houda Bahig, Samuel Kadoury
Summary: In radiation oncology, predicting patient risk stratification using deep learning is important for improving patient outcomes. This study proposes a new deep learning model for predicting distant metastasis, locoregional recurrence, and overall survival probabilities in head and neck cancer patients. The model achieves high accuracy and can be applied to multi-center data.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Marketa Hudeckova, Vladimir Koucky, Jan Rottenberg, Bretislav Gal
Summary: Liquid biopsy for detecting gene mutations in ctDNA can be used for early diagnosis of HNSCC, with TP53 being the most commonly explored mutated gene. More studies are needed to verify these conclusions and translate them into clinical practice.
Article
Oncology
Alexander Ruehle, Carmen Stromberger, Erik Haehl, Carolin Senger, Helene David, Raluca Stoian, Constantinos Zamboglou, Andreas Knopf, Volker Budach, Anca-Ligia Grosu, Nils H. Nicolay
Summary: A novel prognostic score and nomogram were established based on easily accessible clinical data for elderly head-and-neck squamous cell carcinoma patients undergoing (chemo)radiotherapy. The score differentiated between different prognostic groups based on reduced performance status, increased comorbidity burden, and elevated CRP levels. The developed tools may assist in decision-making for this vulnerable population.
RADIOTHERAPY AND ONCOLOGY
(2021)
Article
Oncology
Heleen Bollen, Julie van der Veen, Annouschka Laenen, Sandra Nuyts
Summary: IMRT and VMAT have become standard of care in treating head and neck cancer. By analyzing treatment failure patterns, this study indirectly investigated the quality of target volume delineation and treatment delivery, which were performed accurately with few recurrences outside high-dose regions and no marginal failures observed.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Biplab Sarkar, Anusheel Munshi, Tharmarnadar Ganesh, Arjunan Manikandan, Bidhu K. Mohanti
Meeting Abstract
Oncology
B. Sarkar, K. Rastogi, A. Munshi, T. Ganesh, K. Bansal, S. Tiwari, B. B. Tyagi, B. K. Mohanti
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2019)
Editorial Material
Oncology
Bidhu K. Mohanti
PRACTICAL RADIATION ONCOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Arif Jamal Siddiqui, Sadaf Jahan, Syed Amir Ashraf, Mousa Alreshidi, Mohammad Saquib Ashraf, Mitesh Patel, Mejdi Snoussi, Ritu Singh, Mohd Adnan
Summary: The global spread of the new coronavirus infection, identified as novel SARS-CoV-2 and causing COVID-19, has resulted in over 8,807,398 confirmed cases and 464,483 deaths as of June 21, 2020. Currently, there is no specific drug therapy available for COVID-19. Potential antimalarial and antiviral drugs are being tested worldwide, with some showing promising effects against the virus.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Review
Biochemistry & Molecular Biology
Syed Amir Ashraf, Abd Elmoneim O. Elkhalifa, Arif Jamal Siddiqui, Mitesh Patel, Amir Mahgoub Awadelkareem, Mejdi Snoussi, Mohammad Saquib Ashraf, Mohd Adnan, Sibte Hadi
Article
Biochemistry & Molecular Biology
Mitesh Patel, Mohammad Saquib Ashraf, Arif Jamal Siddiqui, Syed Amir Ashraf, Manojkumar Sachidanandan, Mejdi Snoussi, Mohd Adnan, Sibte Hadi
Article
Biochemistry & Molecular Biology
Vaishali Kapoor, Abhay K. Singh, Calvin D. Lewis, Sapna Deore, Dennis E. Hallahan
Summary: Therapeutic antibodies are effective in treating advanced-stage cancer by activating T-lymphocytes and targeting cancer-specific antigens. Additionally, using radiation therapy can enhance the efficacy of these antibodies and antibody-drug conjugates in cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Minakshi Saikia, Nathan Cheung, Abhay Kumar Singh, Vaishali Kapoor
Summary: Molecules involved in drug resistance can be targeted for better therapeutic efficacies. Research on midkine (MDK) has shown a positive correlation between disease progression and MDK expression in most cancers, indicating its association with multi-drug resistance. MDK, a secretory cytokine found in blood, can be used as a potent biomarker for non-invasive detection of drug resistance in cancer and can be targeted for therapy. This article summarizes the current knowledge on the involvement of MDK in drug resistance and its transcriptional regulation, highlighting its potential as a therapeutic target for cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Abdelbaset Mohamed Elasbali, Waleed Abu Al-Soud, Afnan Elayyan Mousa Elayyan, Ziad H. Al-Oanzi, Hassan H. Alhassan, Bashir M. Mohamed, Hamad H. Alanazi, Mohammad Saquib Ashraf, Shadman Moiz, Mitesh Patel, Mirav Patel, Mohd Adnan
Summary: Traditional cancer treatment faces obstacles such as drug resistance, toxicity, and financial constraints. Phytochemicals that regulate molecular mechanisms are gaining interest as alternative medicine. This study used network pharmacology to explore the regulatory mechanisms of 6-shogaol in cervical cancer treatment. Various databases were screened to identify target genes of 6-shogaol and disease-related targets. Protein-protein interactions and topology analysis identified hub genes. Gene ontology and pathway enrichment analysis were conducted, and molecular docking and survival analysis revealed potential target genes for treatment. The study provides scientific evidence supporting the use of 6-shogaol as a treatment for cervical cancer.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Virology
Bharti Datten, Anis Ahmad Chaudhary, Shalini Sharma, Lokender Singh, Krishna Dutta Rawat, Mohammad Saquib Ashraf, Lina M. Alneghery, Malak O. Aladwani, Hassan Ahmad Rudayni, Deen Dayal, Sanjay Kumar, Kundan Kumar Chaubey
Summary: The lumpy skin disease virus (LSDV) infects cattle and buffalo, causing lumpy skin disease (LSD) characterized by enlarged lymph nodes and the formation of cutaneous nodules. Symptoms include high temperature, drop in milk supply, discharge from the eyes and nose, salivation, loss of appetite, depression, damaged hides, and emaciation.
Review
Green & Sustainable Science & Technology
Atreyi Pramanik, Anis Ahmad Chaudhary, Aashna Sinha, Kundan Kumar Chaubey, Mohammad Saquib Ashraf, Nosiba Suliman Basher, Hassan Ahmad Rudayni, Deen Dayal, Sanjay Kumar
Summary: The advancement of industrialization and urbanization has led to various issues, including increased energy demand, excessive waste output, and negative environmental consequences. To mitigate the harmful effects of fossil-derived fuels, researchers are exploring the production and application of biofuels, which are produced from crops and emit lower pollution compared to fossil fuels. However, the high operational costs and limited production volume are major limitations in this process. Recent studies have investigated the use of nanocatalysts in biofuel production to overcome these challenges. This review discusses the application of nanocatalysts in different stages of biofuel production and their advantages and disadvantages.
Article
Infectious Diseases
Amir Mahgoub Awadelkareem, Arif Jamal Siddiqui, Emira Noumi, Syed Amir Ashraf, Sibte Hadi, Mejdi Snoussi, Riadh Badraoui, Fevzi Bardakci, Mohammad Saquib Ashraf, Corina Danciu, Mitesh Patel, Mohd Adnan
Summary: In recent years, there has been a global threat to human health and environment due to the development of antimicrobial resistance (AMR) in bacterial pathogens. In this study, silver nanoparticles derived from Lactobacillus rhamnosus (AgNPs-LR) were tested against three Gram-negative bacteria to determine their effects on biofilm formation and virulence factors controlled by quorum sensing (QS). The results demonstrated that AgNPs-LR effectively inhibited the development of biofilms and QS-mediated virulent traits of these bacteria.
Article
Plant Sciences
Saheem Ahmad, Mohammed Alrouji, Sharif Alhajlah, Othman Alomeir, Ramendra Pati Pandey, Mohammad Saquib Ashraf, Shafeeque Ahmad, Saif Khan
Summary: This study aims to investigate the therapeutic potential of C. nocturnum leaf extracts against diabetes and neurological disorders through inhibitory activities on alpha-amylase and acetylcholinesterase (AChE). Computational molecular docking studies support the inhibitory potential of C. nocturnum leaves-derived secondary metabolites on alpha-amylase and AChE. The methanolic fraction of the leaf extract showed strong antioxidant activity and inhibited alpha-amylase and AChE in a non-competitive and competitive manner, respectively. In silico analysis revealed high-affinity binding of compounds in the methanolic extract to the active sites of alpha-amylase and AChE.
Article
Pharmacology & Pharmacy
Mohammad Saquib Ashraf, Abdulaziz Alsharif, Mazen Almehmadi, Ahad Amer Alsaiari, Mamdouh Allahyani, Abdulelah Aljuaid, Mohammad Asif
Summary: Rhizomes of Curculigo orchioides Gaerten. (Hypoxidaceae) were extracted with hydroalcoholic solvent and separated into alkaloidal and non-alkaloidal fractions. Both fractions showed dose-dependent anti-inflammatory effects, with the alkaloidal fraction being more effective in HRBC method and the non-alkaloidal fraction being more effective in PD method. The study also identified various phytochemicals in C. orchioides that may contribute to its anti-inflammatory action.
LATIN AMERICAN JOURNAL OF PHARMACY
(2023)
Article
Oncology
Suman Bhasker, Rituparna Biswas, Alok Thakar, Subhash Chander, Ahitagni Biswas, Atul Sharma, Anirban Halder, Srikanth Anne, Bidhu K. Mohanti
INDIAN JOURNAL OF SURGICAL ONCOLOGY
(2020)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)