期刊
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷 32, 期 2, 页码 419-425出版社
SCIENCE PRESS
DOI: 10.1007/s00343-014-3124-4
关键词
identification; cytochrome oxidase subunit I (COI); Crassostrea; oyster; high-resolution melting (HRM)
资金
- National Basic Research Program of China (973 Program) [2010CB126402]
- National Natural Science Foundation of China [40730845, 41206149]
- Shandong Provincial Natural Science Foundation [ZR2010DQ024]
- National High Technology Research and Development Program of China (863 Program) [2012AA10A405]
- Earmarked Fund for Modern Agro-Industry Technology Research System [CARS-48]
- Taishan Scholar Program of Shandong Province
- Taishan Scholar Climbing Program of Shandong Province
The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetic studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I (COI) of five common Crassostrea species in China (including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas) and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers (Oyster-COI-1), we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species.
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