期刊
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷 32, 期 2, 页码 377-389出版社
SCIENCE PRESS
DOI: 10.1007/s00343-014-3130-6
关键词
Saccharina japonica; Rubisco; photosynthetic genes; transcription; protein expression; carboxylase activity
资金
- Agriculture Science AMP
- Technology Achievements Transformation Fund [2011GB24910005]
- Modern Agricultural-Industry Technology Research Project [200903030]
- National High Technology Research and Development Program of China (863 Program) [2012AA10A406]
- Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project
- National Twelfth Five-Year Plan for Science AMP
- Technology Support [2013BAB01B01]
Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments, and consequently may have highly efficient ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) activity for carbon assimilation. In our study, we cloned the full-length Rubisco gene from S. japonica (SJ-rbc). It contained an open reading frame for a large subunit gene (SJ - rbcL) of 1 467 bp, a small subunit gene (SJ-rbcS) of 420 bp, and a SJ-rbcL/S intergenic spacer of 269 bp. The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa, 5.81 and 15.84 kDa, 4.71, respectively. After induction with 1 mmol/L isopropyl-beta-D-thiogalactopyranoside for 5 h and purification by Ni2+ affinity chromatography, electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein. Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night. This observation implied that Rubisco played a role in normal gametophytic growth and development. In juvenile sporophytes, mRNA levels of SJ-rbcL, carbonic anhydrase, Calvin-Benson-Bassham cycle-related enzyme, and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance. Similarly, expression of these genes was up-regulated under blue light irradiance at 350 mu mol/(m(2)center dot s). Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据