Article
Multidisciplinary Sciences
Xiaobo Yan, Junjiang Yang, Xiaohui Dong, Beiping Tan, Shuang Zhang, Shuyan Chi, Qihui Yang, Hongyu Liu, Yuanzhi Yang
Summary: The study aimed to explore the optimum protein requirement of juvenile grouper. Results showed that as the protein level increased, there were changes in growth, feed utilization, and nutrient metabolism. The optimum protein level for juvenile grouper was found to be 521.84 g/kg.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yue-Xuan Wang, Sui-Feng Xu, Ye-Wen Wang, Yun-Xiang Jiang, Qi-Wei Qin, Shi-Na Wei
Summary: This study aimed to investigate the protective effect of curcumin against Singapore grouper iridovirus-induced intestinal damage in orange-spotted groupers. The results showed that curcumin significantly alleviated intestine damage, repaired intestinal barrier dysfunction, suppressed intestinal cells apoptosis and inflammatory response, protected intestinal cells from oxidative injury, and restored the disruption of the intestinal microbiota caused by the virus. These findings provide a theoretical basis for the use of curcumin in aquaculture to prevent SGIV-induced intestinal injury.
Article
Fisheries
Honglin Han, Liqun Wang, Suifeng Xu, Shaowen Wang, Min Yang, Chengzong Han, Qiwei Qin, Shina Wei
Summary: In this study, Ec-MARCO was identified and characterized in the orange-spotted grouper, and its important role in regulating the immune response against SGIV infection was demonstrated.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Xiong Li, Hongping Chen, Yihao Cen, Jiule Wang, Na Ni, Xueming Dan, Zequan Mo, Yanwei Li
Summary: This study identified three immunogenic immobilization antigen genes (CI-IA1, CI-IA2, and CI-IA8) from Cryptocaryon irritans. Vaccination with these recombinant antigens elicited a high immobilization titer and protected grouper against C. irritans infection. The findings suggest that these antigens have potential for vaccine development.
Article
Biochemistry & Molecular Biology
Xi Wu, Yang Yang, Chaoyue Zhong, Tong Wang, Yanhong Deng, Hengjin Huang, Haoran Lin, Zining Meng, Xiaochun Liu
Summary: The study focused on ~9500 testicular cells from male, orange-spotted grouper, dividing them into nine clusters and defining ten cell types, including populations with characteristics of male germ cells and somatic cells. The expression patterns of four marker genes were identified in both the ovary and adult testis, providing a blueprint of male germ cells and supporting somatic cells for further cell identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Zhenyu Liang, Shuping Zhang, Zihong Zou, Jinze Li, Rimin Wu, Liqun Xia, Gang Shi, Jia Cai, Jufen Tang, Jichang Jian
Summary: In this study, the researchers characterized a BAG3 homolog from orange-spotted grouper and investigated its roles during viral infection. They found that BAG3 plays an important role in modulating virus-induced cell death, regulating the expression of interferon and inflammation-related factors, and influences the immune response of fish during viral infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Dapeng Yu, Guanjian Yang, Hongli Xia, Zhen Gan, Zhiwen Wang, Liqun Xia, Kevin W. H. Kwok, Jia Cai, Yishan Lu
Summary: The present study aimed to investigate the pathogenesis of DSS-induced enteritis in Orange-spotted grouper (Epinephelus coioides). Fish were challenged with 200 μl 3% DSS via oral irrigation and feeding, using an appropriate dosage based on the disease activity index. The results showed that DSS-induced inflammatory responses were closely associated with the expression of pro-inflammatory cytokines, NF-kappa B, and myeloperoxidase (MPO) activity. Histological examination and SEM analysis revealed severe intestinal lesions, inflammatory cell infiltration, and microvillus effacement. The injured intestinal villi gradually recovered during the experimental period of 18 days. These findings provide valuable insights into the pathogenesis of enteritis in farmed fish, contributing to the control of enteritis in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yinghui Qin, Haixiang Liu, Peipei Zhang, Si Deng, Reng Qiu, Lunguang Yao
Summary: In this study, a STAT2 gene from orange-spotted grouper was cloned and characterized. The findings suggest that EcSTAT2 exerts antiviral activity against red-spotted grouper nervous necrosis virus through up-regulation of host interferon response.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yuexuan Wang, Suifeng Xu, Yewen Wang, Xuchao Zhang, Jiatao Chen, Qiwei Qin, Shina Wei
Summary: This study found that curcumin, extracted from the traditional Chinese medicine turmeric, has various biological activities, including immunoenhancement, antioxidant, and antibacterial properties. The results showed that curcumin can enhance fish immunity, reduce mortality caused by viral infection, and repair liver injury caused by the virus. This provides a theoretical foundation for the functional development of curcumin in aquaculture.
Article
Fisheries
Zihong Zou, Qi Zheng, Jia Cai, Jufen Tang, Liqun Xia, Pengfei Li, Jichang Jian
Summary: This study characterized a BAG5 homolog gene from orange-spotted grouper and found that it can inhibit viral replication by regulating the expression of interferon and inflammation-related factors. These findings provide valuable insights into the immune response against viral infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Shina Wei, Honglin Han, Suifeng Xu, Youhua Huang, Jingguang Wei, Qiwei Qin
Summary: This study identified and characterized Ec-CysB in orange-spotted grouper, which plays a crucial role in the immune response. The results suggest that Ec-CysB affects viral replication and virus-induced cell apoptosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Immunology
Mengshi Sun, Siting Wu, Shaozhu Kang, Jiaming Liao, Luhao Zhang, Zhuqing Xu, Hong Chen, Linting Xu, Xin Zhang, Qiwei Qin, Jingguang Wei
Summary: This study found that the infection of red-spotted grouper nervous necrosis virus (RGNNV) induced the formation of stress granules (SGs) in grouper spleen cells. The SGs particles co-located with the classic SG marker protein eIF3 eta, and some SGs depolymerized under treatment with a translation inhibitor. Inhibition of the PERK kinase significantly inhibited the formation of SGs and decreased the phosphorylation of eIF2 alpha. Overexpression of G3BP1 in Epinephelus coioides inhibited the replication of RGNNV by up-regulating the interferon and inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Environmental
Qing Wang, Xuzhuo Duan, Fengqi Huang, Huitao Cheng, Chunli Zhang, Lihua Li, Xinhe Ruan, Qi He, Huirong Yang, Wenbiao Niu, Qiwei Qin, Huihong Zhao
Summary: The study showed that polystyrene nanoplastics (PS-NPs) can impair the function of marine organisms' digestive system, intestinal flora, immune system, and nervous system. It also found that PS-NPs uptake can lead to cytotoxicity, increased viral replication, and decreased resistance to viruses in cells and tissues, providing new insights into the impact of PS-NPs on marine fish.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Zhifeng Ye, Tingting Zhao, Qianhao Wei, Haoran Lin, Yong Zhang, Shuisheng Li
Summary: This study investigated the expression of vitellogenin and estrogen receptors in the liver of orange-spotted grouper. The results showed that their expression levels increased significantly during the breeding season and were upregulated by estradiol (E-2) injection. ER beta 2 was found to mediate the E-2-induced expression of vitellogenin and ER alpha, and the transcriptional activity of ER alpha was enhanced through a classical ERE-dependent pathway. This study provides insights into the roles of ER subtypes in VTG expression and the efficient VTG production in orange-spotted grouper during the breeding season.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Xinxin Shan, Tengfei Xu, Zhiyuan Ma, Xinhui Zhang, Zhiqiang Ruan, Jieming Chen, Qiong Shi, Xinxin You
Summary: This study aimed to improve the ammonia tolerance of groupers through genomic selection, predicting the genomic estimated breeding values using genome-wide loci and single nucleotide polymorphisms. Results showed that the method based on GWAS-informative SNPs performed better in cost-efficiency for genomic selection.
