Article
Chemistry, Physical
Jia-Chun Lin, Yan Yi, Lan Zhang, Ran Cang, Xiao-Jun Ji, Zhi-Gang Zhang
Summary: This study developed a biocatalytic method for the oxidation of aldehydes to carboxylic acids in the aqueous phase using a newly discovered whole-cell D. xibeiensis R13 biocatalyst. The method showed high yield and selectivity, while avoiding the use of toxic transition-metal reagents, making it a green and sustainable alternative.
MOLECULAR CATALYSIS
(2023)
Article
Energy & Fuels
Manishi Tripathi, Saurabh Kumar, Govind Makarana, Reeta Goel
Summary: The contamination of agricultural land with heavy metals is a global concern, as it affects the quality and safety of agricultural products. This study investigates the effectiveness of Pseudomonas putida KNP9 in removing and accumulating lead and cadmium, and enhancing soybean growth in metal-contaminated soil. The results show that KNP9 has high tolerance and bioaccumulation potential for both metals, and its inoculation significantly improves the growth of soybean plants under metal stress. Therefore, the inoculation of metal bioaccumulating bacterium KNP9 in soybean shows promise for enhancing biodiesel production in metal-contaminated sites.
Article
Chemistry, Physical
Reinhard Oeggl, Juliane Glaser, Eric von Lieres, Dorte Rother
Summary: The implementation of biocatalysis in flow chemistry aligns with green chemistry principles, but high substrate concentrations are often hindered by solubility issues or toxicity. Continuous synthesis of (S)-2-HPP from benzaldehyde and acetaldehyde in a micro-aqueous system requires spatial distribution of acetaldehyde to maintain sub-toxic levels.
CATALYSIS SCIENCE & TECHNOLOGY
(2021)
Article
Agronomy
Stefania Fontanazza, Alessia Restuccia, Giovanni Mauromicale, Aurelio Scavo, Cristina Abbate
Summary: The study aimed to identify microorganisms responsible for degrading BP mulch films through a combination of biodegradation and molecular tests. A field trial revealed a higher abundance of Pseudomonas putida in BP plots, showing potential for accelerating the degradation of BP mulch films and reducing plastic pollution in agriculture.
Article
Ecology
Magali de la Cruz Barron, Ellen van Velzen, Uli Kluemper, Markus Weitere, Thomas U. U. Berendonk, David Kneis
Summary: Predation defense is a crucial aspect of predator-prey interactions, which brings complexity to ecosystem dynamics. Prey organisms have developed various strategies to evade predation, including different modes, reversibility, and scope of defenses. However, there is still limited understanding of the ecological and evolutionary factors that affect long-term predator-prey dynamics, especially in terms of trade-offs and interactions between alternative defenses in prey populations evolving under predation pressure.
Article
Microbiology
Hadiastri Kusumawardhani, Rohola Hosseini, Jo-Anne Verschoor, Johannes H. de Winde
Summary: Bacterial plasmids carry various heavy metal and antibiotic resistance genes, degradation pathways, and transporter genes for organic solvents or aromatic compounds. This study focused on the analysis of gene clusters on the megaplasmid pTTS12 from Pseudomonas putida S12 and revealed its involvement in stress responses and the presence of transposon-flanked cassettes mediating heavy metal resistance. The study provides insights into the modular structure of pTTS12 as an IncP-2 plasmid and its potential application in biotechnology.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agricultural Engineering
Shunli Zhang, Cuiluan Ma, Qi Li, Qing Li, Yu-Cai He
Summary: This study confirmed the feasibility of converting D-fructose to BHMF through one-pot chemoenzymatic conversion, achieving high yields. Pseudomonas Putida S12 was shown to efficiently catalyze the transformation of D-fructose-valorized 5-HMF into BHMF in a benign reaction system.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Madison D. Bygd, Kelly G. Aukema, Jack E. Richman, Lawrence P. Wackett
Summary: Thousands of organofluorine chemicals are known to be persistent and toxic environmental pollutants, but few bacteria biodegrade fluorinated chemicals. A rapid screening method was developed to discover new organofluoride biodegradation, revealing 21 new microbial defluorination reactions. A general mechanism was delineated for the biodegradation of trifluoromethylphenyl groups that are increasingly being used in drugs and pesticides.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Madison D. Bygd, Kelly G. Aukema, Jack E. Richman, Lawrence P. Wackett
Summary: Perfluorinated carbon atoms in a diether linkage are commonly found in commercial anesthetics, drugs, fungicides, and insecticides. Pseudomonas putida F1 can rapidly defluorinate DFBD with the key enzyme toluene dioxygenase. This mechanism may have implications for the biodegradation of DFBD-containing compounds in the environment.
Article
Biology
Carlos Arellano-Caicedo, Pelle Ohlsson, Martin Bengtsson, Jason P. Beech, Edith C. Hammer
Summary: This study used microfluidic devices to investigate how spatial distortion of pore spaces affects fungal and bacterial growth, interactions, and substrate degradation. Results showed that sharper angles reduced fungal and bacterial biomass, especially when angles were repeated. Modifications to fungal habitats were identified as a potential cause for this effect.
COMMUNICATIONS BIOLOGY
(2021)
Article
Environmental Sciences
Emmanuel Oliver Fenibo, Ramganesh Selvarajan, Akebe Luther King Abia, Tonderayi Matambo
Summary: Hydrocarbon footprints in the environment affect the ecosystem and result in imbalances that lead to human diseases and reduced biodiversity. Microbial bioremediation using functional genes, such as the bacterial alkB gene, is a sustainable option for cleaning hydrocarbon-impacted environments. However, relying solely on alkB detection as a monitoring parameter for hydrocarbon degradation is erroneous due to its distribution among bacteria and limitations in linking copy number to contamination concentration levels.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
A. J. Toribio, F. Suarez-Estrella, M. M. Jurado, J. A. Lopez-Gonzalez, M. R. Martinez-Gallardo, M. J. Lopez
Summary: This study focused on the establishment of consortia using heterotrophic bacteria and cyanobacteria and their effectiveness in the development of tomato seedlings. The results showed that certain microbial combinations can promote plant growth, with a particularly significant effect on aerial development.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Oscar Puiggene, Maria Jose Cardenas Espinosa, Dietmar Schlosser, Stephan Thies, Nico Jehmlich, Uwe Kappelmeyer, Stephan Schreiber, Daniel Wibberg, Joern Kalinowski, Hauke Harms, Hermann J. Heipieper, Christian Eberlein
Summary: A strain of Pseudomonas capeferrum TDA1, isolated from a plastic dump site, has been found to possess the ability to degrade polyurethane (PU) compounds. The degradation pathway and mechanisms for PU degradation in this organism have been further explored.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Multidisciplinary
Fathiah Mohamed Zuki, Robert G. J. Edyvean, Hamed Pourzolfaghar, Norherdawati Kasim
Summary: The study reveals that a horizontal approach of a capsule-shaped bacterium to a mineral surface is more attractive compared to a vertical approach, and the orientation of the bacterium as well as the type and topology of the mineral influence the adhesion of the bacteria to the surface.
Review
Biochemistry & Molecular Biology
Lei Ai, Fangwei Mei, Ren Peng
Summary: This review aims to summarize the role of membrane proteins in microbial organic solvent tolerance and propose strategies and challenges for improving the protective function of membrane proteins in organic solvent stress. Membrane proteins related to transporter, signal transduction, and material and energy metabolism are involved in solvent tolerance, and optimization of their expression level and engineering are used to tackle solvent toxicity. Membrane proteins play a crucial role in microbial solvent tolerance.
PROTEIN AND PEPTIDE LETTERS
(2023)
Article
Green & Sustainable Science & Technology
Jorge Senan-Salinas, Alberto Blanco, Raquel Garcia-Pacheco, Junkal Landaburu-Aguirre, Eloy Garcia-Calvo
Summary: The study evaluates the potential of direct recycling of EoL RO modules in terms of environmental and economic aspects. Results show significant environmental benefits of recycling, but long-distance transport may limit the distribution of recycled products.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Biochemical Research Methods
Sara Jimenez-Jimenez, Georgiana Amariei, Karina Boltes, Maria Angeles Garcia, Maria Luisa Marina
Summary: A novel CE methodology was developed for the enantiomeric separation of panthenol, with optimal conditions determined through experimental investigation. The method showed good performance for quantitating dexpanthenol in cosmetic and pharmaceutical formulations, meeting the requirements of ICH guidelines.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Physical
L. Rodriguez-Saez, J. Landaburu-Aguirre, S. Molina, M. C. Garcia-Payo, E. Garcia-Calvo
Summary: The surface modification of recycled ultrafiltration membranes was conducted using statistical design of experiments with dip-coating catechol and polyethyleneimine, resulting in improved permeability and flux recovery ratio at mild conditions. Statistical design of experiments proved to be a useful tool in understanding the factors affecting surface modification.
SURFACES AND INTERFACES
(2021)
Article
Biochemical Research Methods
Sara Jimenez-Jimenez, Georgiana Amariei, Karina Boltes, Maria Angeles Garcia, Maria Luisa Marina
Summary: An Electrokinetic Chromatography method was developed for the stereoselective analysis of sulfoxaflor, achieving baseline separation of its four stereoisomers. The method showed good performance for commercial agrochemical analysis and sulfoxaflor toxicity prediction.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Microbiology
Lorena Penacoba-Antona, Jorge Senan-Salinas, Arantxa Aguirre-Sierra, Pedro Leton, Juan Jose Salas, Eloy Garcia-Calvo, Abraham Esteve-Nunez
Summary: The study evaluated two operation modes of a full-scale METland in wastewater treatment, with the aerobic mode showing the highest removal rates for organic pollutants and nitrogen, and being the most environmentally friendly design. METland demonstrated higher efficiency in wastewater treatment compared to constructed wetland, with an increased capacity per surface area without external energy use.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Chemical
Jorge Contreras-Martinez, Carmen Garcia-Payo, Paula Arribas, Laura Rodriguez-Saez, Amaia Lejarazu-Larranaga, Eloy Garcia-Calvo, Mohamed Khayet
Summary: End-of-life reverse osmosis membranes can be repurposed for forward osmosis applications as recycled FO and transformed recycled FO membranes, with the latter showing superior performance in terms of water permeate fluxes. The transformation process involves interfacial polymerization to further enhance FO membrane performance. This study demonstrates the feasibility of utilizing discarded RO membranes for wastewater treatment in FO technology, contributing to circular economy and sustainability in membrane science.
Article
Environmental Sciences
M. Greno, G. Amariei, K. Boltes, M. Castro-Puyana, M. A. Garcia, M. L. Marina
Summary: In this study, the separation of tetramethrin isomers using cyclodextrin-micellar electrokinetic chromatography was successfully achieved for the first time. The ecotoxicity of tetramethrin on aquatic microinvertebrates was evaluated, revealing its toxic effects on Daphnia magna. The oxidative stress-mediated mode of action for tetramethrin on Daphnia magna was reported as the first evidence in this work.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Mabel Diaz-Cubilla, Pedro Leton, Carlos Luna-Vazquez, Marta Marron-Romera, Karina Boltes
Summary: This study evaluated the impact of partially degraded pharmaceuticals and personal care products on anaerobic biomass metabolism. The findings showed that these compounds had a negative effect on anaerobic digestion process, leading to microbial stress and cell damage.
Article
Biochemistry & Molecular Biology
Amaia Lejarazu-Larranaga, Juan M. Ortiz, Serena Molina, Sylwin Pawlowski, Claudia F. Galinha, Vanessa Otero, Eloy Garcia-Calvo, Svetlozar Velizarov, Joao G. Crespo
Summary: This work explores the application of reverse osmosis upcycled membranes in Donnan Dialysis and ion exchange membrane bioreactor processes for nitrate removal from water. Spectroscopy techniques were used to evaluate the performance and changes of the membranes, and the technical feasibility of using upcycled AEMs was validated. This membrane recycling concept can also be applied for the removal and recovery of other target negatively charged species.
Article
Biochemistry & Molecular Biology
Laura Rodriguez-Saez, Sotiris I. Patsios, Jorge Senan-Salinas, Junkal Landaburu-Aguirre, Serena Molina, Eloy Garcia-Calvo
Summary: This study explores the feasibility of using recycled ultrafiltration membranes as submerged flat-sheet membranes in an aerobic membrane bioreactor for the first time. The results show that the recycled ultrafiltration membranes perform similarly to commercial membranes in terms of rejection performance, fouling behavior, and permeate quality. The recycled membranes exhibit higher fouling resistance and have the potential to be competitively used in industrial applications, as indicated by a preliminary economic assessment.
Article
Engineering, Environmental
Jorge Senan-Salinas, Junkal Landaburu-Aguirre, Jorge Contreras-Martinez, Eloy Garcia-Calvo
Summary: Recycling end-of-life RO membrane modules into FO membranes is an innovative approach to integrate membrane science into Circular Economy. This study assessed the environmental potential of this strategy and compared it with commercial TFC and CTA membranes. The results showed the interest in most impact categories and identified solvent losses as a source of error.
RESOURCES CONSERVATION AND RECYCLING
(2022)
Article
Biochemistry & Molecular Biology
Anamary Pompa-Pernia, Serena Molina, Amaia Lejarazu-Larranaga, Junkal Landaburu-Aguirre, Eloy Garcia-Calvo
Summary: This study validated the application capability of recycled nanofiltration (rNF) membranes and recycled anion-exchange membranes (rAEMs) obtained from end-of-life reverse osmosis (RO) membranes in saline wastewater treatment. Recycled membranes provide a cost-effective and environmentally friendly option by integrating membrane-based technology into a circular economy model. The results showed that rNF membranes exhibited high selective rejection of divalent ions, while rAEMs demonstrated appropriate demineralization rate in comparison to commercial membranes without compromising power consumption.
Article
Biochemistry & Molecular Biology
Jorge Senan-Salinas, Junkal Landaburu-Aguirre, Raquel Garcia-Pacheco, Eloy Garcia-Calvo
Summary: This study conducted an environmental analysis of the entire life cycle of recycled nanofiltration (r-NF) membranes for the first time, emphasizing the importance of energy use during the usage stage. By evaluating different strategies and energy sources, the optimal method to improve the permeability of r-NF membranes was identified.
Article
Engineering, Civil
Jeny A. Larrea-Murrell, Tristano Bacchetti-De-Gregoris, Mayra Heydrich-Perez, Daysi Lugo-Moya, Abraham Esteve-Nunez, Karina Boltes-Espinola, Marcia M. Rojas-Badia
Summary: The study investigated the influence of various physicochemical and microbiological indicators on bacterial assemblages in a freshwater ecosystem in a Biosphere Reserve. The results showed seasonal variation in bacterial community at less polluted stations, with total dissolved solids, E. coli concentration and dissolved oxygen significantly affecting bacterial profiles. The combination of environmental variables and DGGE molecular data revealed the impact of chemical and microbial contamination on bacterial communities in the San Juan River.
TECNOLOGIA Y CIENCIAS DEL AGUA
(2021)