Article
Chemistry, Multidisciplinary
Audrey Picard-Lafond, Dominic Lariviere, Denis Boudreau
Summary: Metal-enhanced fluorescence is a powerful tool that improves the brightness and photostability of target-responsive fluorophores, making it useful in the design of sensitive chemical sensors. Expanding the use of metal-enhanced fluorescence to the detection of low-level inorganic pollutants, such as monitoring mercury in the environment, is an important goal. This study demonstrates the potential of metal-enhanced fluorescence for heavy metal detection through fluorescence sensing, by developing a Hg2+-sensitive nanoprobe.
Article
Biochemistry & Molecular Biology
Michelle S. Frei, Birgit Koch, Julien Hiblot, Kai Johnsson
Summary: Fluorescence lifetime multiplexing using fluorescent probes with distinct lifetimes but similar spectral properties enables multi-channel imaging in living cells. Among 18 synthetic probes, we identified 8 pairwise combinations suitable for multiplex imaging. Combining self-labeling protein tags, we can image up to 8 different biological targets, effectively increasing the number of observable targets. The combination of synthetic probes and fluorescence lifetime multiplexing is a powerful live-cell imaging technique.
ACS CHEMICAL BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Shahid Ali, Muhammad Mansha, Nadeem Baig, Safyan Akram Khan
Summary: Emissions of mercury from industrial waste and natural sources have toxic effects on organisms and ecosystems, even in trace amounts. Despite advancements in mercury detection sensors, challenges such as difficult deployment in remote areas and low robustness and selectivity in harsh environments remain. Future perspectives include the selective detection of organomercury species, which are highly toxic and reactive in aquatic environments.
Article
Multidisciplinary Sciences
Takeru Takagi, Tasuku Ueno, Keisuke Ikawa, Daisuke Asanuma, Yusuke Nomura, Shin-nosuke Uno, Toru Komatsu, Mako Kamiya, Kenjiro Hanaoka, Chika Okimura, Yoshiaki Iwadate, Kenzo Hirose, Tetsuo Nagano, Kaoru Sugimura, Yasuteru Urano
Summary: The discovery of a new actin-binding small molecule presents an important advance in the field, offering the opportunity to design and synthesize new functional molecules. This molecule enables visualization of the actin cytoskeleton and allows for highly specific control of actin filaments in living cells.
Article
Chemistry, Analytical
Shaoheng Li, Shi Feng, Xuerong Song, Qingchuan Zheng, Guodong Feng, Zhiguang Song
Summary: Biological thiols, including cysteine, homocysteine, and glutathione, play important roles in physiological and pathological processes. Abnormal concentration of these thiols can lead to various diseases. In this study, a series of fluorescent molecules based on benzotriazole and coumarin were designed and synthesized. The molecule BTAC(e) showed high selectivity towards homocysteine and could react with it to enhance fluorescence. The probe also exhibited excellent selectivity towards homocysteine compared to cysteine and glutathione. The fluorescence intensity had a linear relationship with the concentration of homocysteine and the probe could be used for imaging homocysteine in living cells.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Xue Tian, Lloyd C. Murfin, Luling Wu, Simon E. Lewis, Tony D. James
Summary: Small-molecule based fluorescent probes play an increasingly important role in detecting and imaging biological signaling molecules due to their simplicity, high selectivity, and real-time analysis capabilities. Various sensing mechanisms, including FRET, ICT, PeT, ESIPT, AIE, as well as dual/triple sensing mechanisms, are highlighted, but challenges remain. Future development should focus on improving small-molecule fluorescent probes for biosensing applications.
Review
Biochemistry & Molecular Biology
Mayeul Collot, Sonia Pfister, Andrey S. Klymchenko
Summary: In this paper, the latest advances in fluorescent plasma membrane (PM) probes for chemical and biophysical sensing as well as for super-resolution imaging are reviewed.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Li Xiao, Kaiqi Liu, Lian Duan, Xinjian Cheng
Summary: Two types of fluorescent functionalized silk probes were prepared using silk as the substrate, which can specifically detect Hg2+ and Ag+ with dual-channel identification. The probes with two terminal C-C/C C bonds showed higher sensitivity and excellent recognition ability, with a detection limit as low as 0.25 μM.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)
Review
Chemistry, Inorganic & Nuclear
Conor Geraghty, Conor Wynne, Robert B. P. Elmes
Summary: Fluorescent probes are essential tools in studying biological systems, and the 1,8-naphthalimide fluorophore is commonly used for detecting enzymatic conversions. With advancements in synthetic methods, the sensitivity and selectivity of these probes continue to improve.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Chemistry, Multidisciplinary
Luanjing Li, Jiahe Wang, Shihan Xu, Chunxia Li, Biao Dong
Summary: This paper reviews the recent research progress and applications of metal ion fluorescent probes, and discusses the future development direction.
FRONTIERS IN CHEMISTRY
(2022)
Review
Chemistry, Inorganic & Nuclear
Vinayak Juvekar, Sang Jun Park, Juyoung Yoon, Hwan Myung Kim
Summary: Two-photon microscopy combined with two-photon fluorescent probes enables visualization of metal ion distribution in live tissues, facilitating the development of probes for metal ion detection. Recent progress between 2014 and 2020, including design strategies, history, and specific applications, was summarized in this review.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Biochemistry & Molecular Biology
Sachin B. Wagh, Vladimir A. Maslivetc, James J. La Clair, Alexander Kornienko
Summary: Fluorescent probes are widely used in various fields, and the development of new experimental designs has facilitated innovation in probe discovery methods.
Article
Chemistry, Organic
Veselin Nasufovic, Patrick Then, Fabian Droge, Michael Duong, Christoph Kaether, Benjamin Dietzek, Rainer Heintzmann, Hans-Dieter Arndt
Summary: Efficient synthesis of silicon-rhodamines has enabled the preparation and evaluation of silicon-rhodamine isothiocyanate (SITC) as a novel tool for fluorescent labeling. Demonstrating high stability and excellent photophysical properties, SITC-actin was found to be neutral to F-actin polymerization induction, making it well-suited for high resolution fluorescence microscopy.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Brian E. Fratto, Emma L. Culver, Gabriel Davis, Robert Deans, John B. Goods, Sean Hwang, Nicole K. Keller, John A. Lawrence, Alexander R. Petty, Timothy M. Swager, Joseph J. Walish, Zhengguo Zhu, Jason R. Cox
Summary: This paper reports on a method that enables a smartphone to perform laboratory quality time-resolved analysis of luminescent samples. By making small modifications to the smartphone and utilizing existing image capture applications and analysis software, this approach demonstrates the analytical potential of smartphones in tasks such as authentication, encryption, packaging, compounds, and physical phenomena. This method highlights the power of repurposing existing technologies to expand the reach and inclusivity of scientific exploration, creating new avenues for data collection and analysis.
Article
Chemistry, Multidisciplinary
Ines J. Marques, Pedro D. Vaz, Ana V. Girao, Mariela M. Nolasco, Carla D. Nunes
Summary: Silica-coated iron oxide and silica nanoparticles were prepared and derivatized with a europium complex to yield magneto-fluorescent and fluorescent nanomaterials. The fluorescence was enhanced by the anchored europium complex and maintained in cellular medium. These nanomaterials have potential applications as biosensors and in imaging.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Yuqing Yang, Irene Yang, Mingnan Cao, Zheng-yuan Su, Renyi Wu, Yue Guo, Mingzhu Fang, Ah-Ng Kong
Article
Oncology
Yue Guo, Renyi Wu, John M. Gaspar, Davit Sargsyan, Zheng-Yuan Su, Chengyue Zhang, Linbo Gao, David Cheng, Wenji Li, Chao Wang, Ran Yin, Mingzhu Fang, Michael P. Verzi, Ronald P. Hart, Ah-Ng Kong
Article
Chemistry, Medicinal
Wenji Li, Zheng-Yuan Su, Yue Guo, Chengyue Zhang, Renyi Wu, Linbo Gao, Xi Zheng, Zhi-Yun Du, Kun Zhang, Ah-Ng Kong
CHEMICAL RESEARCH IN TOXICOLOGY
(2018)
Correction
Chemistry, Multidisciplinary
Tianlu Wang, Tingting Hong, Tun Tang, Qjanqian Zhai, Xiwen Xing, Wuxiang Mao, Xiaolong Zheng, Liang Xu, Jinjun Wu, Xiaocheng Weng, Shaoru Wang, Tian Tian, Bifeng Yuan, Bing Huang, Lin Zhuang, Xiang Zhou
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Mingzhu Liu, Xiaolong Zheng, Veronica Grebe, Mingxin He, David J. Pine, Marcus Weck
Summary: This study successfully achieved different morphologies of colloidal superstructures by tuning the particles' patch sizes and assembly conditions, proposing an empirical phase diagram that provides guidance in designing two-dimensional materials with desired superstructures. Our strategy may be applicable to the assembly of three-dimensional colloidal structures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Rong Huang, Xian-Dong Zhang, Xing Wu, Ji-Kai Liu
Summary: The novel pH-activatable fluorescent probe based on beta-carboline derivatives, L-1, displays significant fluorescent response towards pH variation with high selectivity, good photo-stability, and favorable pKa value. Moreover, L-1 can dynamically monitor the release of protons during ester hydrolysis reaction in consistent with enzymatic kinetics manner.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Chemistry, Medicinal
Li Zhou, Xing Wu, Fu Yang, Minghao Zhang, Rong Huang, Jikai Liu
Summary: Phospholipids (PLs) from Antarctic krill oil were purified with high purity, containing various molecular species with omega-3 fatty acids, displaying good anti-inflammatory activity by reducing inflammatory mediators in cells through multiple pathways.
Article
Chemistry, Multidisciplinary
Hui-Xiang Yang, Jin-Tao Ma, Juan He, Zheng-Hui Li, Rong Huang, Tao Feng, Ji-Kai Liu
Summary: Four polyketide-amino acid derivatives, pardinumones A-D, were isolated from the wild mushroom Tricholoma pardinum. They exhibited antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli with MIC values in the range of 6.25-50 μg/mL.
Article
Nanoscience & Nanotechnology
Rong Huang, Qi-Hang Yu, Xue-Di Yao, Wen-Long Liu, Yin-Jia Cheng, Yi-Han Ma, Ai-Qing Zhang, Si-Yong Qin
Summary: An antibiotic-free strategy combining peptide-drug therapy with chemodynamic therapy was developed to effectively inhibit bacterial growth, showing potential in addressing superbug challenges and multidrug-resistant strains.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Organic
Liang Tu, Limei Gao, Qiang Wang, Zhixing Cao, Rong Huang, Yongsheng Zheng, Jikai Liu
Summary: A fundamentally new reaction model of benzofuran-derived azadienes (BDAs) has led to the synthesis of biologically important pyrazoles through a tandem [3 + 2] cycloaddition/ring-opening rearrangement reaction with nitrile imines. The nature and type of substrates determine two distinct reaction pathways, and minor modifications to the substrates allow access to spiro-pyrazolines.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ruige Cao, Xing Wu, Hui Guo, Xin Pan, Rong Huang, Gangqiang Wang, Jikai Liu
Summary: The study demonstrated that naringin significantly alleviated symptoms of ulcerative colitis by inhibiting inflammatory response and regulating intestinal microbiota, which could potentially be a promising natural therapeutic agent for the dietary treatment of UC and improvement of intestinal symbiosis.
Review
Oncology
Ruipeng Mu, Jiaqi Yuan, Yue Huang, John K. Meissen, Si Mou, Meina Liang, Anton I. Rosenbaum
Summary: In recent years, the development of new modalities in biotherapeutics, such as bioconjugates and novel routes of delivery, has posed challenges for bioanalytical methods. The complexity of bioconjugates makes their quantification crucial for drug discovery and development. Additionally, the potential for in vivo catabolism and biotransformation adds further complexity, requiring thorough investigations in multiple biological matrices. Furthermore, the evaluation and use of excipients to enhance absorption in oral and inhaled biotherapeutics require risk-benefit assessments. Therefore, bioanalytical methods that can measure excipients and potential drug metabolites in biological matrices are highly relevant in addressing these emerging bioanalysis challenges.
Article
Engineering, Biomedical
Qi-Hang Yu, Rong Huang, Kai-Yue Wu, Xiao-Le Han, Yin-Jia Cheng, Wen-Long Liu, Ai-Qing Zhang, Si-Yong Qin
Summary: This study developed acidity-triggered nanotherapeutics with adaptable geometrical morphology for bacterial targeting and optimized therapy. The nanotherapeutics could reverse its charge and destabilize bacterial membranes in acidic infection sites, and the transformation of morphology enhanced the binding with bacterial membranes.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Tingting Yao, Xiaowei Xu, Rong Huang
Summary: This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have advantages such as high yield, harmless by-products, and simple reaction conditions, allowing for easy and effective molecular diversity.
Review
Pharmacology & Pharmacy
Christina N. Ramirez, Wenji Li, Chengyue Zhang, Renyi Wu, Shan Su, Chao Wang, Linbo Gao, Ran Yin, Ah-Ng Tony Kong
