期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 5, 期 9, 页码 1952-1961出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201000279
关键词
biosensors; nanopores; peptides; proteins; single-molecule analysis
资金
- National Natural Science Foundation of China [20875030]
- Shuguang Project of Shanghai [07SG36]
As a unique technique at the singe-molecule level to explore the distribution and temporal order of events, nanopore technology has attracted increasing attention. In comparison to the previous applications in DNA sequencing, this Focus Review highlights the technical details of biological nanopores, especially a-hemolysin, in the analysis of peptides and proteins. The instrument configurations, experimental interferences, and data analysis including the conformation of peptides and proteins and their interactions for single-molecule detection are discussed.
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