Article
Chemistry, Multidisciplinary
Benjamin W. Lewis, Niels Bisballe, Marco Santella, Peter A. Summers, Jean-Baptiste Vannier, Marina K. Kuimova, Bo W. Laursen, Ramon Vilar
Summary: This study expanded the library of triangulenium probes and investigated the interactions of two new molecules featuring ethyl-morpholino substituents with DNA topologies. However, despite modifications to the core structure, these two probes were unable to distinguish between DNA topologies based on fluorescence lifetime.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ji-Dong Shen, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: In this study, a novel fluorescent probe Rho-IDA-CoII was synthesized and successfully used to label and detect His-tagged proteins. The recombinant Bacillus subtilis strain capable of secreting NHase was screened using this probe, and high NHase activity was achieved.
Article
Biophysics
Fei Yan, Shengui He, Xiuyan Han, Jiayue Wang, Xiangge Tian, Chao Wang, Tony D. James, Jingnan Cui, Xiaochi Ma, Lei Feng
Summary: Tuberculosis is a major threat to public health globally. The usage of β-lactam antibiotics in treating TB is limited due to the presence of β-lactamase in Mycobacterium tuberculosis, which causes antibiotic resistance. However, combining β-lactam antibiotics with a β-lactamase inhibitor can overcome this resistance. In this study, a near-infrared fluorescent probe LXMB was developed for real-time detection and imaging of Blac activity in Mtb. A high-throughput screening platform was established using LXMB to search for Blac inhibitors from herbal medicines. Tannic acid was identified as a potential Blac inhibitor and showed enhanced inhibitory effects when combined with β-lactam antibiotics against M. tuberculosis H37Ra. This research provides valuable insights into the mechanism of drug resistance and contributes to the development of new TB treatments.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Sanjoy Kumar Sheet, Monosh Rabha, Bhaskar Sen, Sumit Kumar Patra, Kripamoy Aguan, Snehadrinarayan Khatua
Summary: A novel fluorescence probe for selective detection of G-quadruplex (GQ) DNA has been synthesized and applied in monitoring RNase H activity. The probe exhibited high selectivity for GQ DNA structure, as demonstrated by experimental and spectroscopic results, enabling detection of target DNA through changes in luminescence intensity.
Article
Chemistry, Medicinal
Medve Laura, Gealageas Ronan, Lam Bao Vy, Guillaume Valentin, Castillo-Aguilera Omar, Camberlein Virgyl, Catherine Piveteau, Rosell Melissa, Fleau Charlotte, Warenghem Sandrine, Charton Julie, Dumont-Ryckembusch Julie, Bosc Damien, Leroux Florence, van Endert Peter, Deprez Benoit, Deprez-Poulain Rebecca
Summary: ERAP2 is an emerging pharmacological target in cancer immunotherapy and autoinflammatory disease control, and selective inhibitors and activators have been identified in this study, which could serve as useful starting points for further optimization.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Samara Martin-Alonso, Dongwei Kang, Javier Martinez del Rio, Joanna Luczkowiak, Estrella Frutos-Beltran, Lina Zhang, Xiqiang Cheng, Xinyong Liu, Peng Zhan, Luis Menendez-Arias
Summary: In retroviruses, strand displacement DNA-dependent DNA polymerization catalyzed by viral reverse transcriptase (RT) is crucial for synthesizing double-stranded proviral DNA. This study demonstrates that the loss of RNase H activity in HIV-1 RT does not significantly affect strand displacement during DNA template copying but has a major impact on DNA polymerization during reactions with RNA templates. The findings suggest potential applications in transcriptomics technologies and the development of novel antiretroviral agents.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Physical
Ze Yu, Qiuying Li, Qian Ma, Wenpeng Ye, Zhongfu An, Huili Ma
Summary: Electro-fluorescent materials with 100% internal quantum efficiency and short fluorescence lifetimes can be achieved by harvesting high-lying triplet excitons. In this study, two excited-state descriptors, triplet-triplet energy gap ΔETT and oscillator strength f, were established to characterize exciton utilization efficiency, photoluminescence quantum yield, and fluorescence lifetime. High-throughput screening of over 5000 fluorophores predicted 19 candidate molecules with a high IQE (>90%), guided by these descriptors.
CHEMISTRY OF MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ryan E. Johnson, Makay T. Murray, Lucas J. Bycraft, Stacey D. Wetmore, Richard A. Manderville
Summary: A simple and efficient method for the synthesis of fluorescent molecular rotors (FMRs) is reported, which involves on-strand aldehyde capture using 6-hydroxy-indanone as a handle. The resulting internal FMR chalcones demonstrate high quantum yields, large Stokes shifts, and bright emissions in the visible region, making them suitable for various biotechnological applications.
Article
Chemistry, Multidisciplinary
Min Gao, Sun Hyeok Lee, Raj Kumar Das, Haw-Young Kwon, Heon Seok Kim, Young-Tae Chang
Summary: The development of a live B-cell selective small molecule probe CDyB has been achieved through unbiased fluorescence library screening. The molecular target of CDyB is identified as the SLC35C2 transporter. The fluorescence of CDyB and the expression of SLC35C2 are positively correlated with B-cell maturation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Sneh M. Toprani, Dimitrios Bitounis, Qiansheng Huang, Nathalia Oliveira, Kee Woei Ng, Chor Yong Tay, Zachary D. Nagel, Philip Demokritou
Summary: The potential genotoxic effects of engineered nanomaterials (ENMs) on DNA repair pathways have been investigated using a fluorescence multiplex-host-cell reactivation (FM-HCR) assay. Results showed that some ENMs may enhance DNA repair capacity while others suppress it. This method can be a valuable part of a multitier, in vitro hazard assessment of ENMs, providing insights into the interplay between ENM properties, DNA repair efficiency, and genomic stability.
Article
Chemistry, Multidisciplinary
Ruike Wang, Chen Zong, Gairu Li, Junhong Wang, Tiantian Kong, Fei Li, Junmin Chang
Summary: A novel fluorescence imaging platform was developed for ultra-sensitive and rapid detection of cardiac troponin T, utilizing a high-throughput immunosensor chip and a DNA dendrimer capped with fluorescent dyes. The platform demonstrated excellent detection capabilities in serum samples, providing a new approach for early diagnosis of acute myocardial infarction.
Article
Environmental Sciences
Hao Yang, Yumei Liu, Yi Wan, Yi Dong, Qiang He, Mohammad Rizwan Khan, Rosa Busquets, Guiping He, Jiaqi Zhang, Ruijie Deng, Zhifeng Zhao
Summary: We report a DNAzyme-templated exponential isothermal amplification strategy (DNAzymee), which can detect lead (Pb2+) pollution with high sensitivity and screen microbial biosorbents for lead removal. The specific recognition of Pb2+ by DNAzyme can be amplified by exponential isothermal amplification reaction (EXPAR) and monitored using a G-quadruplex specific dye. The designed assay showed a low limit of detection (95 pM) and accurately identified Pb2+ pollution in different real samples. The screening of Pb2+ biosorbents revealed Leuconostoc mesenteroides as a promising candidate for lead removal. Therefore, the DNAzymee assay can be used as a platform to monitor lead pollution and screen efficient biosorbents.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Sina Zhang, Ye Xie, Yulong Li, Jiali Ren, Ting Deng, Jishan Li
Summary: A novel DNAzyme-based paper sensor was developed for detecting RNase H activity, showing a linear detection range of 5 to 60 U/mL with a limit of detection of 1.2 U/mL under optimized conditions. This paper sensor was able to detect RNase H in complex biosamples, providing a rapid and sensitive method for RNase H detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biology
Charanya Kumar, Sahil Batra, Jack D. Griffith, Dirk Remus
Summary: R-loops can lead to replication stress, but the impact can be suppressed by RNase H1 and Pif1. Replisomes have the ability to maintain fork progression in certain conditions, providing a mechanistic basis for distinguishing harmful from non-harmful R-loops.
Article
Chemistry, Analytical
Li Guan, Yanyan Zhou, Xiao Li, Yongbao Mao, Anyang Li, Yile Fu, Wen Liu, Sheying Dong, Zhen Liang, Yukui Zhang, Qun Zhao, Lihua Zhang
Summary: A universal strategy has been developed to synthesize pentamethine cyanines with different aromatic heterocycle substituents on the meso-polymethine chain, leading to the development of specific fluorescent probes with high sensitivity and robust OFF/ON switching for targeting G4s. These probes exhibit self-quenching in aqueous solution due to H-aggregation, but can be triggered to emit fluorescence upon recognition of G-quadruplexes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Enguo Ju, Faming Wang, Zhenzhen Wang, Chaoying Liu, Kai Dong, Fang Pu, Jinsong Ren, Xiaogang Qu
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Chemistry, Physical
Hao Qiu, Fang Pu, Zhengwei Liu, Xuemeng Liu, Kai Dong, Chaoqun Liu, Jinsong Ren, Xiaogang Qu
Article
Chemistry, Physical
Fang Pu, Songrong Qu, Hao Qiu, Lu Zhang
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2020)
Article
Chemistry, Analytical
Hao Liu, Huan Wang, Lu Zhang, Yanjuan Sang, Fang Pu, Jinsong Ren, Xiaogang Qu
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Multidisciplinary
Hao Liu, Yawen You, Yanjuan Sang, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: The study presents a microRNA-triggered nanozyme cascade platform for enhanced tumor-specific chemodynamic therapy, where the cascade reaction can be successfully triggered by high microRNA expression in cancer cells to generate more (OH)-O-. and demonstrate excellent tumor-specific therapeutic performance. This work provides a new dimension for tumor-specific chemodynamic therapy.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Min Yang, Xiaohui Wang, Fang Pu, Ying Liu, Jia Guo, Shuzhuo Chang, Guoying Sun, Yinghua Peng
Summary: This study constructed a nanoplatform by rare earth elements and peptide modification, enhancing the effectiveness of exosomes in targeting tumor therapy, significantly improving the effects of imaging diagnosis and photothermal therapy. The platform could effectively accumulate at cancer sites, increasing the survival rate of mice.
Review
Chemistry, Inorganic & Nuclear
Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: Sensor arrays based on nucleic acids have great potential in detecting multiple targets. However, there is a lack of comprehensive reviews on sensor arrays from the perspective of nucleic acid as sensing elements.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Multidisciplinary Sciences
Yawen You, Qingqing Deng, Yibo Wang, Yanjuan Sang, Guangming Li, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: This study reports on a DNA-based biocompatible, highly efficient, and precisely targeted copper nanocatalyst, which exhibits excellent catalytic activity and achieves efficient prodrug activation and antitumor effects in cancer cells.
NATURE COMMUNICATIONS
(2022)
Correction
Multidisciplinary Sciences
Yawen You, Qingqing Deng, Yibo Wang, Yanjuan Sang, Guangming Li, Fang Pu, Jinsong Ren, Xiaogang Qu
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Jiawei Zhu, Yawen You, Wenting Zhang, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: In order to boost the endogenous Cu(I) level for intracellular drug synthesis through the bioorthogonal reaction, a self-adaptive bioorthogonal catalysis system was constructed by encapsulating prodrugs and sodium ascorbate within adenosine triphosphate aptamer-functionalized metal-organic framework nanoparticles. The system presents specificity to tumor cells and does not require exogenous Cu catalysts, thereby leading to high anti-tumor efficacy and minimal side effects both in vitro and in vivo. This work will open up a new opportunity for developing biosafe and high-performance bioorthogonal catalysis systems.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Guangming Li, Hao Liu, Jiadai Yi, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: A versatile fiber-based compartmentalization strategy is developed to construct multi-nanozyme system capable of simultaneously performing incompatible reactions. Different microenvironments are tailored to each nanozyme, allowing them to exhibit their highest activity. This strategy has potential applications in disease treatment and biosensing.
Article
Chemistry, Multidisciplinary
Minhao Jiang, Xiangyu Yan, Yibo Wang, Fang Pu, Huiling Liu, Yumei Li, Chunfeng Yang, Jiawei Zhu, Xuemeng Liu, Jinsong Ren, Xiaogang Qu
Summary: A one-component sensor array that mimics natural gustatory system is constructed for accurate and high-throughput discrimination and identification of versatile analytes. The system shows universality by rapidly and accurately discriminating metal ions, proteins, bacteria, and classifying antibiotic mechanisms. This study expands the application scope of HOFs and provides a facile and universal system for sensing applications.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yawen You, Hao Liu, Jiawei Zhu, Yibo Wang, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: This study developed a DNAzyme-augmented and targeted bioorthogonal catalyst that exhibits specificity to cancer cells and promotes the generation of Cu(i) for in situ drug synthesis. The DNAzyme also damages cancer cells, resulting in synergistic cancer therapy.
Article
Chemistry, Multidisciplinary
Xiang Ran, Zhenzhen Wang, Fang Pu, Enguo Ju, Jinsong Ren, Xiaogang Qu
Summary: A novel probe based on Au nanoclusters with aggregation-induced emission property was developed for sensitive telomerase detection in living cells and solid tumor tissue. This study provided insight into the specific connection mechanism of metal nanoclusters for AIE generation and shows great potential for disease detection both in vitro and in vivo.
MATERIALS HORIZONS
(2021)
Article
Chemistry, Multidisciplinary
Can Xu, Fang Pu, Jinsong Ren, Xiaogang Qu
CHEMICAL COMMUNICATIONS
(2020)