Article
Biotechnology & Applied Microbiology
Xi Xie, Pei Wu, Xiaochen Huang, WenFeng Bai, Bowen Li, Ning Shi
Summary: Developing a novel solubilizing fusion tag XXA has shown improved soluble expression of inclusion bodies and highlighted the potential of using antifreeze protein as a solubilizing fusion tag for the first time.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Rosa Maria Chura-Chambi, Chuck Shaker Farah, Ligia Morganti
Summary: We have demonstrated that hGH-IBs possess native-like secondary and tertiary structures and that non-denaturing methods can lead to high yields of refolded protein. The refolding process described in this study is likely applicable to other proteins, and it may be particularly useful for reducing the pH required for alkaline solubilization.
MICROBIAL CELL FACTORIES
(2022)
Review
Biotechnology & Applied Microbiology
Robert Klausser, Julian Kopp, Eva Prada Brichtova, Florian Gisperg, Mohamed Elshazly, Oliver Spadiut
Summary: Throughout the 21st century, the understanding of inclusion bodies (IBs) has shifted to considering them as a targeted production strategy for recombinant proteins, rather than unwanted by-products. Although IBs can be easily separated and provide a highly pure product, additional steps for solubilization and refolding are necessary. Developing solubilization techniques that maintain correctly folded protein structures without denaturation is a current challenge. Ionic liquids have emerged as promising agents for solubilization, but their adoption in industry is hindered by various challenges. Mechanistic models based on protein unfolding thermodynamics and molecular dynamics simulations are suggested as a potential solution.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Vivek Rajendran, S. Pushpavanam, Guhan Jayaraman
Summary: Chromatography-based refolding offers advantages over dilution-refolding in terms of reducing aggregate formation and enabling refolding at higher protein concentration. By using a 3-column Periodic Counter-current Chromatographic system for continuous refolding, it is possible to improve resin utilization and reduce specific buffer consumption, ultimately leading to higher recovery and throughput.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biotechnology & Applied Microbiology
Jasvinder Kaur, Anupam Singh, Amulya K. Panda, Rup Lal
Summary: LinB is an important haloalkane dehalogenase mainly involved in degrading the notorious 13-HCH in the isomers of hexachlorocyclohexane (HCH). Studies found that the enzyme activity on 13-HCH is significantly affected by a mutation in the active site.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qi Wang, Chun Zhang, Zenglan Li, Fangxia Guo, Jing Zhang, Yongdong Liu, Zhiguo Su
Summary: The study successfully refolded the highly hydrophobic protein rhIFN beta-lb IBs using a strategy of high hydrostatic pressure combined with additives, achieving a good yield. The resulting product showed similar structures and in vitro bioactivity to the standard product.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Xuefeng Li, Baorong Zhang, Quan Hu, Changchao Chen, Jiahua Huang, Lu Liu, Shengbin Wang
Summary: Fusion tag-induced formation of inclusion bodies provides a powerful means to express unstructured or toxic proteins. Our study illustrated that the aggregation hot spots in a fusion tag played important roles in mediating its insoluble expression. Efficient production of inclusion bodies could also be implemented by refining its primary structure to form a more stable beta-sheet with higher hydrophobicity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
Yu Nan Zhang, Jia Jian Liu, Wei Zhang, Han Yu Qin, Lin Tao Wang, Yuan Yuan Chen, Li Yuan, Fen Yang, Rong Yue Cao, Xue Jun Wang
Summary: In this study, a simple and cost-effective method was developed to obtain high amounts of functional MMP-2 protein, which would contribute to the progress of studies on the biological activity of this important proteinase. Furthermore, this protocol can be applied for the expression, purification, and refolding of other bacterial toxic proteins.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Article
Agronomy
Jing Yang, Yuanyu Zhang, Guojie Xing, Jia Wei, Lu Niu, Qianqian Zhao, Qinan Cai, Xiaofang Zhong, Xiangdong Yang
Summary: Soybean seeds have great potential as a safe and cost-effective host for large-scale production of biopharmaceuticals and industrially important macromolecules. This study focused on improving the yield of target proteins in soybean seeds through polypeptide fusion and RNA interference, which significantly increased the accumulation levels of recombinant proteins without affecting the total protein content or protein/oil ratio in soybean seeds.
Article
Biochemical Research Methods
Peipei Chang, Xiaoyun Li, Jingye Lin, Cong Li, Sen Li
Summary: This article describes a novel method for the renaturation and purification of inclusion bodies, which significantly increases the activity recovery of bioactive proteins.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Svetlana Bakholdina, Anna M. Stenkova, Evgenia P. Bystritskaya, Evgeniy Sidorin, Natalya Yu Kim, Ekaterina S. Menchinskaya, Tatiana Yu Gorpenchenko, Dmitry L. Aminin, Nikita A. Shved, Tamara F. Solov'eva
Summary: The study showed that cultivation temperature influences the conformational quality of Yersinia pseudotuberculosis phospholipase A(1) (PldA) in inclusion bodies, with optimal fluorescence and expression observed at 18 degrees C. Solubilization of PldA-GFP from inclusion bodies in urea and SDS leads to denaturation, revealing structural heterogeneity. The use of GFP as a fusion partner improves conformational quality but negatively impacts enzymatic activity.
Article
Multidisciplinary Sciences
Bastian Heim, Rene Handrick, Marcus D. Hartmann, Hans Kiefer
Summary: This study presents a generic approach to assess and optimize GPCR refolding from E. coli inclusion bodies through thermostability analysis. By adapting a technique based on cysteins exposed upon thermal denaturation with fluorescent dye CPM, successful expression, purification and refolding of GPCRs S1P(1) and GPR3 were demonstrated. Refolded receptors were then subjected to lipidic cubic phase crystallization screening.
Article
Biochemistry & Molecular Biology
Priyank Singhvi, Ankit Saneja, Rahul Ahuja, Amulya K. Panda
Summary: A novel formulation comprised of trifluoroethanol, n-propanol, and urea disrupts protein aggregation forces, enabling high solubilization and refolding efficiency for recovering bioactive proteins from bacterial inclusion bodies. Extensive comparative studies demonstrate a 50% yield of bioactive proteins with the capability to disrupt tertiary protein structures and protect secondary structures, reducing soluble aggregate formation during refolding. This formulation eliminates the need for screening and optimizing solubilization formulations, improving efficiency in recovering bioactive proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Irene Barguilla, Ugutz Unzueta, Jose Vicente Carratala, Olivia Cano-Garrido, Antonio Villaverde, Alba Hernandez, Neus Ferrer-Miralles
Summary: Bacterial inclusion bodies (IBs) are protein complexes that appear under stress conditions and recent research has shown their potential as biomaterials for cell growth promotion. In this study, the cytotoxicity, oxidative stress, and genotoxicity of different protein formats were analyzed in eukaryotic cells. The results demonstrate that natural IBs and soluble protein nanoparticles are non-toxic materials for eukaryotic cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Carla Lizbeth Segovia-Trinidad, Bastian Quaas, Zhaopeng Li, Antonina Lavrentieva, Yvonne Roger, Thomas Scheper, Andrea Hoffmann, Ursula Rinas
Summary: Smad8 is a transcriptional regulator in the TGF-beta signaling pathway, with cSmad8 showing constitutive activity and tendon cell inducing potential. By engineering cSmad8 to include the HIV TAT signal, researchers successfully achieved cellular internalization and demonstrated biological activity in C3H10T1/2 cells.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Biochemical Research Methods
Florian Mayer, Monika Cserjan-Puschmann, Benedikt Haslinger, Anton Shpylovyi, Christian Sam, Miroslav Soos, Rainer Hahn, Gerald Striedner
Summary: The scale-up of bioprocesses remains a major challenge in the biotechnology industry. This study presents the design and characterization of a scale-down setup that combines a plug-flow reactor with a stirred-tank bioreactor. The setup was evaluated using computational fluid dynamics simulations and was tested on high-cell-density Escherichia coli cultivations. The results showed that the setup reduced biomass and product yields during scale-down cultivations, but increased the intracellular fraction of the target product. The flexibility of the setup, combined with CFD simulations, makes it a valuable tool for studying scale effects at the laboratory scale.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemical Research Methods
Leo A. A. Jakob, Tomas Mesurado, Alois Jungbauer, Nico Lingg
Summary: The CASPON enzyme is an interesting enzyme for fusion protein processing due to its generation of an authentic N-terminus. However, the high cysteine content in CASPON may lead to aggregation through disulfide-bond formation, which can negatively impact enzymatic activity. Different multimerization states of CASPON were isolated and analyzed to determine their propensity for multimerization and enzymatic activity. The results showed that the dimer state was the most stable and active, with 50% higher enzymatic activity than the tetramer state. Multimerization was mainly mediated by cysteine, and attractive protein-protein interactions were observed in the presence of ammonium sulfate and on a chromatographic stationary phase. These findings provide insights into protein purification and highlight the importance of controlling multimerization to maintain protein activity.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Baubek Spanov, Oladapo Olaleye, Tomes Mesurado, Natalia Govorukhina, Alois Jungbauer, Nico C. van de Merbel, Nico Lingg, Rainer Bischoff
Summary: The study found that Pertuzumab undergoes changes in charge heterogeneity, primarily due to deamidation in the Fc domain and N-terminal pyroglutamate formation in the heavy chain. Peptide mapping analysis also showed that clinical samples had 2-3% deamidation in the heavy chain CDR2, 20-25% deamidation in the Fc domain, and 10-15% N-terminal pyroglutamate formation in the heavy chain.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Magdalena Pappenreiter, Benjamin Bayer, Marijan Logarusic, Bernhard Sissolak, Alois Jungbauer
Summary: The study investigated the individual impacts of mild and rigorous intra-experimental parameter shifts on the culture performance and cell metabolism of an antibody producing CHO cell line. The results showed significant changes in cell growth, metabolism, and productivity due to intra-experimental parameter shifts. However, if the conditions within an experiment are changed, rigorous parameter shifts should be avoided, as they can lead to irreversible changes in cell behavior, as observed in media blending experiments.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Viktoria Mayer, Anna-Carina Frank, Shirin Preinsperger, Patrick Csar, Petra Steppert, Alois Jungbauer, Patricia Pereira Aguilar
Summary: Host cell DNA, especially chromatin, is an important impurity in downstream processing of enveloped viruses. Endonuclease treatment, using salt-tolerant endonucleases, combined with flow-through chromatography using core-shell particles, showed high purity and purification efficiency in the processing of recombinant measles virus. This strategy has the potential to be applied in other viral platforms.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Biochemistry & Molecular Biology
Georg Csukovich, Nina Kramer, Barbara Pratscher, Ivana Gotic, Patricia Freund, Rainer Hahn, Gottfried Himmler, Sabine Brandt, Iwan Anton Burgener
Summary: Given the high prevalence of intestinal disease in humans and animals, a study has been conducted to establish a canine organoid system and test the neutralising effects of recombinant and natural antibodies on C. difficile toxins. The results showed that only the recombinant antibodies effectively neutralised the toxins, suggesting the potential of canine intestinal organoids in testing different components and complex interactions between cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Magdalena Pappenreiter, Hubert Schwarz, Bernhard Sissolak, Alois Jungbauer, Veronique Chotteau
Summary: This study tested different process set ups and conditions in low shear TFF systems using magnetic levitation pumps in small-scale perfusion bioreactors. The authors validated a novel concept of applying reverse flow across the hollow fiber using two magnetically levitating pumps. The results showed that product sieving could be improved by 30% with the application of dynamic recirculation flow, and minimal product retention was achieved by reversing the flow of two alternating pumps with short cycle times. Additionally, a correlation was found between the passage of high molecular weight species and the flow direction, as well as the degree of product sieving.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Jürgen Beck, Matthias Biechele, Christoph Repik, Petra Gruber, Paul G. Furtmueller, Rainer Hahn
Summary: This study systematically compared the elution behavior of plasmid DNA on three common anion exchange resins. It was found that plasmid DNA consistently elutes at a characteristic salt concentration in linear gradient elution, and elutes only above this concentration in isocratic elution. Structural analysis supported this explanation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Gabriele Recanati, Magdalena Pappenreiter, Christoph Gstoettner, Patrick Scheidl, Elena Dominguez Vega, Bernhard Sissolak, Alois Jungbauer
Summary: Continuous precipitation coupled with continuous tangential flow filtration is an economical method for capturing recombinant antibodies from cell culture supernatant. By adopting tubular reactors to replace surge tanks, a continuous harvest and mass flow of product can be achieved with a narrow residence time distribution. The combination of CaCl2 precipitation and PEG capture method allows for efficient removal of host-cell DNA and retention of glycosylation profile.
ENGINEERING IN LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Juergen Beck, Georg Hochdaninger, Giorgio Carta, Rainer Hahn
Summary: This study evaluates the influence of resin structure on the competitive binding and separation of a two-component protein mixture with anion exchange resins. It compares different types of resins and demonstrates the importance of the resin's intra-particle structure and its effects on diffusional transport for efficient separation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Markus Mozgovicz, Andreas Fischer, Cecile Brocard, Alois Jungbauer, Nico Lingg
Summary: Immobilized metal affinity chromatography (IMAC) is a powerful technique for capturing and purifying biopharmaceuticals. Protein recovery can be compromised due to spreading and unfolding of the protein, leading to fouling of the stationary phase. This study investigates the kinetics of irreversible adsorption of a protease on an IMAC resin and suggests that L-arginine sulfate can decelerate the irreversible adsorption.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Nico Lingg, Markus Mozgovicz, Theresa Schaufler, Alexander Prokup, Amy Carruthers, David Boerema, Laura Keigher, Nathan Brinkman, Alois Jungbauer
Summary: Currently, IVIG is manufactured in batch through cold ethanol precipitation, which leads to a slow process with high space requirements. We have developed a continuous method using a tubular reactor to replace the batch precipitation method. This reactor, filled with static mixers for efficient cooling, allows for complete precipitation with the same yield and purity even with a residence time of only one minute. A tubular reactor with specific dimensions can process 4000 L of plasma per day, providing an opportunity to reduce the plant footprint or intensify existing manufacturing plants.
PROCESS BIOCHEMISTRY
(2023)
Article
Engineering, Chemical
Mafalda S. Santos, Christian Schuster, Harald Rennhofer, Helga C. Lichtenegger, Herwig Peterlik, Tim Causon, Alois Jungbauer
Summary: Efficient recovery of building-block chemicals, such as biobased itaconic acid, produced by microbial processes can be achieved using ultrathin membranes. The composition of the membranes was optimized to improve the separation performance, and membranes with high selectivity for itaconic acid were successfully demonstrated in experiments with synthetic solutions and culture supernatant.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Baubek Spanov, Oladapo Olaleye, Tomes Mesurado, Natalia Govorukhina, Alois Jungbauer, Nico C. van de Merbel, Nico Lingg, Rainer Bischoff
Summary: The ion-exchange profile of pertuzumab was evaluated, revealing that deamidation in the Fc domain and N-terminal pyroglutamate formation in the heavy chain were the main contributors to charge heterogeneity. Peptide mapping analysis of clinical samples showed deamidation rates of 2-3% in the heavy chain CDR2, 20-25% in the Fc domain, and N-terminal pyroglutamate formation rates of 10-15% in the heavy chain. These findings suggest that in vitro stress studies can predict in vivo modifications.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Hannah Engelmaier, Christoph Dattenboeck, Katerina Petrushevska-Seebach, Alois Jungbauer, Nikolaus Hammerschmidt
Summary: The study presents an integrated, continuous process for the capture of recombinant blood coagulation factors. The developed process consists of three continuous unit operations and has demonstrated successful continuous capture of the factors over a 24-hour duration.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Engineering, Chemical
Qing Han, Mengqing Shi, Linkai Han, Di Liu, Mingwei Tong, Yuxin Xie, Zhonghua Xiang
Summary: Developing highly efficient bifunctional oxygen electrocatalysts is crucial for zinc-air flow batteries. Metal-organic frameworks (MOFs) and covalent organic polymers (COPs) have emerged as promising alternatives due to their designable and controllable atomic-level structures. However, their catalytic performances are limited by conductivity and catalytic activity. In this study, nanosheet FeNi-MOF and iron phthalocyanine rich COP hybrid materials are assembled through the pi-pi stacking effect to create highly efficient bifunctional electrocatalysts. The resulting catalyst exhibits superior catalytic performance and stability, making it a promising candidate for zinc-air flow batteries.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Daria Grigorash, Dmytro Mihrin, Rene Wugt Larsen, Erling H. Stenby, Wei Yan
Summary: The article introduces a new approach to describe the cross-association between molecules, allowing for the simulation of weakly bound molecular complexes with different conformations in mixtures. By incorporating this approach into the equation of state, accurate predictions of vapor-liquid equilibrium and liquid-liquid equilibrium can be made. The new method is validated through experiments on alcohol and acid mixtures, with the results compared to experimental data, demonstrating its accuracy and reliability.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Mohammed Al-Sharabi, Daniel Markl, Vincenzino Vivacqua, Prince Bawuah, Natalie Maclean, Andrew P. E. York, Axel Zeitler
Summary: This study used terahertz pulsed imaging to investigate the transport process of different solvents into ceramic catalytic materials. The results showed that the heating rate of the samples influenced the water transport rate, while the viscosity of 1-octanol slowed down its transport.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Chukwunonso Anyaoku, Sati Bhattacharya, Rajarathinam Parthasarathy
Summary: This study aimed to enhance understanding of settling dynamics in viscoelastic fluids by developing a semi-empirical correlation and a dimensionless ratio, which accurately described the characteristics of settling suspensions.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Antti I. Koponen, Janika Viitala, Atsushi Tanaka, Baranivignesh Prakash, Olli-Ville Laukkanen, Ari Jasberg
Summary: This study focuses on the development of foam application chemicals for the paper and board industry. The research explores the rheology of the polyvinyl alcohol foam used in the process. Measurements were conducted to determine the foam viscosity and slip flow. The results suggest that slip flow contributes significantly to the total flow rate, and the obtained viscosity and slip models provide a solid foundation for industrial processes.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Dalei Sun, Jinghui Cai, Yating Yang, Zhiwu Liang
Summary: In this study, Fe-doped alpha-Bi2O3 catalysts with different Fe/Bi molar ratios were synthesized and utilized in the carbonylation of isobutyl amine with CO2. The results showed that Fe doping significantly enhanced the catalytic abilities of alpha-Bi2O3.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Yuan Tian, Xinxin Wang, Yanrong Liu, Wenping Hu
Summary: This paper predicts the solubility of nitrogen gas in ionic liquids (ILs) using two quantitative structure-property relationship (QSPR) models. By combining machine learning methods and ionic fragments contribution method, the accuracy and reliability of the prediction models are improved.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Liwang Wang, Wei Liu, Pan Yang, Yulong Chang, Xiaoxu Duan, Lingyu Xiao, Yaoming Hu, Jiwei Wu, Liang Ma, Hualin Wang
Summary: This study investigates the effective phase interfacial area (ae) of hydro-jet cyclones at different injection angles. The results show that a 45 degrees upward incidence angle yields the most favorable flow field characteristics for efficient mass transfer. The significant enhancement in ae of the hydro-jet cyclones offers the advantage of reducing equipment volume and cost savings.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Chuanjun Wu, Jiangzhi Chen, Jiyue Sun, I-Ming Chou, Shenghua Mei, Juezhi Lin, Lei Jiang
Summary: In this study, the solubility of H2S hydrate in water was measured using Raman spectroscopy. The results showed that the solubility increases with temperature under certain equilibrium conditions, and the solubility also depends on pressure and temperature under different equilibrium conditions. A thermodynamic model based on the van der Waals-Platteeuw theory was developed to predict the solubility, demonstrating its accuracy.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Lorenzo Brivio, Serena Meini, Mattia Sponchioni, Davide Moscatelli
Summary: This study investigates the influence of three main parameters and proposes a kinetic model to predict the optimal operating conditions for high yield of dimethyl terephthalate (DMT) in the chemical recycling process of polyethylene terephthalate (PET).
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Hongju Lin, Fanhui Liao, Yanchang Chu, Mingyu Xie, Lun Pan, Yuanyuan Wang, Lijian Leng, Donghai Xu, Le Yang, Gangfeng Ouyang
Summary: A honeycomb NiCo/C-Na catalyst with a micro-meso-macroporous structure has been fabricated and shown to have significantly higher catalytic activity for the decarboxylation of fatty acids. It also proves to be efficient in upgrading sludge HTL bio-crude, resulting in a biofuel with decreased viscosity and increased density.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Xiaoxian Li, Rui Li, Min Lin, Mingde Yang, Yulong Wu
Summary: A series of coated non-noble metal porous carbon catalysts were synthesized and applied to the aqueous-phase deoxygenation of algal bio-oil. One of the catalysts showed excellent deoxygenation selectivity and catalytic activity at 250 degrees C. The catalyst exhibited good hydrothermal stability and the reaction mechanism was proposed based on product analysis and active site analysis.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
M. V. Chudakova, M. V. Popov, P. A. Korovchenko, E. O. Pentsak, A. R. Latypova, P. B. Kurmashov, A. A. Pimenov, E. A. Tsilimbaeva, I. S. Levin, A. G. Bannov, A. V. Kleymenov
Summary: A series of catalysts with different potassium contents were prepared using solution combustion synthesis and characterized using various techniques. The results showed that the potassium content affected the phase composition and texture of the catalysts. The addition of a small amount of potassium resulted in a change in particle size distribution, leading to higher hydrogen yield. The Ni-1%K2O/Al2O3 catalyst exhibited the highest hydrogen yield at temperatures of 675 and 750 degrees Celsius.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Aliakbar Roosta, Nima Rezaei
Summary: In this study, we modified the electrolyte cubic plus association equation of state (e-CPA EoS) and integrated it with two electrical conductivity models to estimate the electrical conductivity of 11 monovalent electrolyte solutions in water. The modified e-CPA model demonstrated better performance and the hybridization with electrical conductivity models resulted in two predictive models for estimating the electrical conduction of dilute and concentrated electrolyte solutions. These predictive models showed relative average percentage deviations (AARD) of 11.15% and 13.87% over wide ranges of temperature and electrolyte concentration.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Haoren Niu, Jianzheng Wang, Qingzhu Jia, Qiang Wang, Jin Zhao, Fangyou Yan
Summary: A study developed two quantitative structure-property relationship models for the complexation performance of alpha- and beta-cyclodextrins and validated their stability and predictive ability through internal and external validation. The models showed robustness and satisfactory performance, as demonstrated by the experimental results and model validations. These models can effectively predict the binding constants between cyclodextrins and various types of molecules, providing valuable tools for cyclodextrin design.
CHEMICAL ENGINEERING SCIENCE
(2024)