Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution

Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol

(2015) Yi Yang et al.   CHEMICAL COMMUNICATIONS

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

(2015) Andrew Currin et al.   CHEMICAL SOCIETY REVIEWS

Improving and repurposing biocatalysts via directed evolution

(2015) Carl A Denard et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Methyltransferases do not work by compression, cratic, or desolvation effects, but by electrostatic preorganization

(2015) Jeronimo Lameira et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Multiscale Modeling of Biological Functions: From Enzymes to Molecular Machines (Nobel Lecture)

(2014) Arieh Warshel   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering the specificity of trehalose phosphorylase as a general strategy for the production of glycosyl phosphates

(2014) Chao Chen et al.   CHEMICAL COMMUNICATIONS

Protein engineering for development of new hydrolytic biocatalysts

(2014) Mikael Widersten   CURRENT OPINION IN CHEMICAL BIOLOGY

Substrate scope and selectivity in offspring to an enzyme subjected to directed evolution

(2014) Cecilia Blikstad et al.   FEBS Journal

DC-Analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites

(2014) Xiong Wang et al.   JOURNAL OF BIOTECHNOLOGY

Extreme Synergistic Mutational Effects in the Directed Evolution of a Baeyer–Villiger Monooxygenase as Catalyst for Asymmetric Sulfoxidation

(2014) Zhi-Gang Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Deracemization of 2-Methyl-1,2,3,4-Tetrahydroquinoline Using Mutant Cyclohexylamine Oxidase Obtained by Iterative Saturation Mutagenesis

(2014) Guangyue Li et al.   ACS Catalysis

Residues Controlling Facial Selectivity in an Alkene Reductase and Semirational Alterations to Create Stereocomplementary Variants

(2014) Adam Z. Walton et al.   ACS Catalysis

The Importance of Additive and Non-Additive Mutational Effects in Protein Engineering

(2013) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Quantum Chemistry as a Tool in Asymmetric Biocatalysis: Limonene Epoxide Hydrolase Test Case

(2013) Maria E. S. Lind et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Systematic Optimization of Interface Interactions Increases the Thermostability of a Multimeric Enzyme

(2013) Andreas Bosshart et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Use of ‘small but smart’ libraries to enhance the enantioselectivity of an esterase fromBacillus stearothermophilustowards tetrahydrofuran-3-yl acetate

(2013) Alberto Nobili et al.   FEBS Journal

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

(2013) Manfred T. Reetz   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Stereoselective Oxidation of Aryl-Substituted Vicinal Diols into Chiral α-Hydroxy Aldehydes by Re-Engineered Propanediol Oxidoreductase

(2013) Cecilia Blikstad et al.   ACS Catalysis

Directed enzyme evolution: beyond the low-hanging fruit

(2012) Moshe Goldsmith et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

QM/MM study of the mechanism of enzymatic limonene 1,2-epoxide hydrolysis

(2011) Q.Q. Hou et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library

(2011) A. G. Sandstrom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates

(2010) Jared C. Lewis et al.   CHEMBIOCHEM

Biocatalysts by evolution

(2010) Christian Jäckel et al.   CURRENT OPINION IN BIOTECHNOLOGY

Optimizing non-natural protein function with directed evolution

(2010) Eric M Brustad et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Manipulating the Stereoselectivity of Limonene Epoxide Hydrolase by Directed Evolution Based on Iterative Saturation Mutagenesis

(2010) Huabao Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of an Enantioselective Epoxide Hydrolase: Uncovering the Source of Enantioselectivity at Each Evolutionary Stage

(2009) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

(2008) A. E. Firth et al.   NUCLEIC ACIDS RESEARCH