期刊
CHEMICAL COMMUNICATIONS
卷 50, 期 58, 页码 7834-7836出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc02202e
关键词
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资金
- UGent (MRP-project 'Ghent BioEconomy')
- EC [265854]
- CSC [2008679003]
- IWT [SB71279, SB73279]
A two-step process is reported for the anomeric phosphorylation of galactose, using trehalose phosphorylase as biocatalyst. The monosaccharide enters this process as acceptor but can subsequently be released from the donor side, thanks to the non-reducing nature of the disaccharide intermediate. A key development was the creation of an optimized enzyme variant that displays a strict specificity (99%) for beta-galactose 1-phosphate as product.
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