Article
Chemistry, Multidisciplinary
Rivkah Rogawski, Adi Rogel, Itai Bloch, Maayan Gal, Amnon Horovitz, Nir London, Michal Sharon
Summary: Researchers have developed a method based on native mass spectrometry for analyzing drug uptake and target engagement in cellular contexts. This approach utilizes direct MS for label-free studies of protein-drug binding in human cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Yoshihisa Okuchi, Joshua Reeves, Soon Seng Ng, Daniel H. Doro, Sergi Junyent, Karen J. Liu, Alicia J. El Haj, Shukry J. Habib
Summary: The Wnt3a protein immobilized on a biocompatible bandage induces directed asymmetric cell division of human skeletal stem cells and promotes bone tissue repair. The transplantation of a bandage containing a Wnt-induced osteogenic tissue model facilitates long-term viability of hSSCs and their progeny, resulting in bone repair in a mouse model of critical-sized calvarial defects. This approach provides insights into human osteogenesis regulation and offers a promising method for delivering human osteogenic constructs for in vivo survival and contribution to bone repair.
Article
Chemistry, Multidisciplinary
Cameron O. Zadeh, Jonah R. Huggins, Deepraj Sarmah, Baylee C. Westbury, William R. Interiano, Micah C. Jordan, S. Ashley Phillips, William B. Dodd, Wesley O. Meredith, Nicholas J. Harold, Cemal Erdem, Marc R. Birtwistle
Summary: Mesowestern blot is a high-throughput Western blotting technique that uses a 3D-printable gel casting mold, eliminating the need for a piezoelectric pipetting instrument. It is compatible with small sample sizes and the standard sample preparation methods. Compared to traditional Western blot, Mesowestern uses semi-dry horizontal gel electrophoresis. The technique has lower molecular weight resolution and higher sample-to-sample variation, making it suitable for qualitative screening applications.
Article
Chemistry, Multidisciplinary
Tobias Schnitzer, Jonas W. Rackl, Helma Wennemers
Summary: This study investigated the properties of tripeptide catalysts in complex mixtures and achieved high reactivity and stereoselectivity by tailoring the conformation of tripeptides. The tripeptide catalysts exhibited remarkable chemoselectivity, even catalyzing reactions in cell lysates.
Article
Biochemical Research Methods
Jessica C. Stark, Thapakorn Jaroentomeechai, Katherine F. Warfel, Jasmine M. Hershewe, Matthew P. DeLisa, Michael C. Jewett
Summary: The authors present a protocol for generating bacterial cell lysates and freeze-dried, cell-free reactions for the synthesis of glycoprotein therapeutics and vaccines. This method addresses the limitation of distributed biomanufacturing platforms in producing robust amounts of glycoproteins, which are critical in biologic development. The protocol allows for rapid production of desired glycoproteins and can be completed within one week. The authors anticipate that these cell-free technologies, along with the comprehensive user manual, will accelerate the development and distribution of glycoprotein therapeutics and vaccines.
Article
Biology
Li-xi Cai, Yuan-qing Lin, Yun-meng Chu, Xiao-pin Chen, Li-xing Liu, Ming Zhang, Guang-ya Zhang
Summary: Many current methods for enzyme purification and immobilization have drawbacks, so a simple technique for direct purification and immobilization of target enzymes from cell lysates was proposed. This technique uses ELPs-SpyCatcher chimera to facilitate the formation of silica carriers within seconds, allowing the target enzymes to be covalently immobilized on these carriers via a spontaneous reaction.
Article
Biotechnology & Applied Microbiology
Qin-Qing Wang, Ming Yang, Jian-Hua Hao, Zai-Chao Ma
Summary: In this study, beet molasses was successfully used as a substrate for isomaltulose production with immobilized sucrose isomerase showing high efficiency. The repeated batch reaction using immobilized SIase demonstrated good reusability and significant cost reduction of feedstock costs. This two-step process has the potential to be an effective and promising method for industrial production and application of isomaltulose as a functional sweetener for sucrose substitute.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Steven R. Strezsak, Penny J. Beuning, Nicholas J. Skizim
Summary: Nucleotides, as building blocks of life, play a crucial role in cell processes and energy metabolism. This study presents an anion exchange chromatography method with improved resolution and selectivity for quantifying nucleotides in biological samples. The method allows flexibility in analyte selectivity and has been successfully applied to study nucleotide pool kinetics in a bacterial cell-free lysate system.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Medicine, General & Internal
Layla Panahipour, Azarakhsh Oladzad Abbasabadi, Reinhard Gruber
Summary: Necrotic cell damage resulting from invasive dental procedures leads to the release of cytoplasmic and membranous components, triggering a response in macrophages. This study tested the potential of necrotic cell lysates from different cell lines to modulate the inflammatory response in macrophages. The results showed that all necrotic cell lysates reduced the expression of IL1 and IL6 in LPS-induced macrophages, supporting the concept that necrotic cell lysates can modulate macrophage inflammation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Engineering, Chemical
Markus C. Berg, Irfan Erdem, Eva Berger, Michael C. Martinetz, Cecile Brocard, Nikolaus Hammerschmidt, Astrid Duerauer, Rainer Hahn
Summary: Efficient removal of impurities via filtration is crucial in biopharmaceutical production. We investigated the impact of host cell-derived impurities on the performance of sterile filters, focusing on the decrease in filter capacity induced by dsDNA. Our findings revealed that dsDNA has a major influence on rapid membrane fouling, particularly in filters with small pore sizes (<0.2 μm). By reducing the size of dsDNA fragments or decreasing the electrostatic interactions with positively charged molecules, filter capacity can be significantly increased. Overall, our study emphasizes the importance of monitoring dsDNA even during the primary recovery of proteins in cell lysates.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Qingyue Luo, Jian Wei, Zhuang Guo, Yonghui Song
Summary: In this study, modified natural zeolite and modified magnetic zeolite were synthesized and found to have excellent phosphorus adsorption and passivation capabilities. The addition of these materials can effectively control the release of pollutants and transform unstable phosphorus into stable phosphorus. Moreover, these materials can reduce the amount of bioavailable phosphorus in sediments. The magnetic zeolite, in particular, shows better effects and can be used as a passivator for internal pollution control.
SEPARATION AND PURIFICATION TECHNOLOGY
(2024)
Article
Cell Biology
Lassina Barro, Pierre-Alain Burnouf, Ming-Li Chou, Ouada Nebie, Yu-Wen Wu, Ming-Sheng Chen, Miryana Radosevic, Folke Knutson, Thierry Burnouf
Summary: Human platelet lysate (HPL) is increasingly recognized as an efficient xeno-free alternative growth medium supplement for clinical-grade isolation and propagation of human cells, especially mesenchymal stromal cells (MSCs) from bone marrow or adipose tissues. Studies consistently show that MSCs expanded in media supplemented with HPL exhibit improved proliferation, clonogenicity, immunophenotype, differentiation capacity, and T-cell immunosuppressive activity.
Article
Automation & Control Systems
Min-Xi Li, Yao Chen, Zeng-Ping Chen, Ru-Qin Yu
Summary: A simple label-free fluorescence sensing method for detecting alkaline phosphatase (ALP) was designed in this study, showing high sensitivity and specificity, as well as strong robustness to matrix effects and background interference effects. The integration of the probe technique-based multivariate standard addition strategy achieved satisfactory quantitative results for ALP in complex biological samples.
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
(2021)
Article
Biotechnology & Applied Microbiology
Wenning Chu, Shriarjun Shastry, Eduardo Barbieri, Raphael Prodromou, Paul Greback-Clarke, Will Smith, Brandyn Moore, Ryan Kilgore, Christopher Cummings, Jennifer Pancorbo, Gary Gilleskie, Michael A. Daniele, Stefano Menegatti
Summary: This study introduces peptide ligands that selectively capture AAVs and release them under mild conditions, providing an alternative to commercially available adsorbents based on camelid antibodies. These peptide-based adsorbents efficiently purify AAVs while maintaining high transduction activity.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Siying Liu, Hai-Sheng Su, Zhengxia Yang, Yun Zhang
Summary: This study developed a rapid and effective method for Ag nanorods synthesis, used in creatinine quantitative analysis and cell classification, achieving discrimination between cancer and normal cells through statistical analysis.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Medicinal
Tamas Yelland, Esther Garcia, Charles Parry, Dominika Kowalczyk, Marta Wojnowska, Andrea Gohlke, Matja Zalar, Kenneth Cameron, Gillian Goodwin, Qing Yu, Peng-Cheng Zhu, Yasmin ElMaghloob, Angelo Pugliese, Lewis Archibald, Andrew Jamieson, Yong Xiang Chen, Duncan McArthur, Justin Bower, Shehab Ismail
Summary: This study presents a novel strategy for targeting RAS by stabilizing its interaction with PDE6D and disrupting its localization. By using designed RAS point mutations, the RAS:PDE6D complex is stabilized, leading to inhibition of oncogenic RAS/ERIC signaling. Additionally, the impact of this strategy appears to be cell-type-dependent.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Carmen M. Dominguez, Miguel Garcia-Chame, Ulrike Mueller, Andreas Kraus, Klavdiya Gordiyenko, Ahmad Itani, Heiko Haschke, Peter Lanzerstorfer, Kersten S. Rabe, Christof M. Niemeyer
Summary: This article reports on the high dynamic interaction between streptavidin and DNA origami nanostructures, and provides detailed insights into this interaction using a novel high-speed atomic force microscope. The study also demonstrates a correlation between this interaction and cellular activation. This work is significant for further standardizing the use of DNA nanostructures in the study of biological processes.
Article
Nanoscience & Nanotechnology
Ahmed E. Zoheir, Laura Meisch, Marta Velaz Martin, Christoph Bickmann, Alexei Kiselev, Florian Lenk, Anne-Kristin Kaster, Kersten S. Rabe, Christof M. Niemeyer
Summary: This study presents the fabrication and application of a novel device for decoding microbial dark matter (MDM) in environmental microbiomes. The device, which is low-cost and easily scalable, allows for rapid colonization and enrichment of microorganisms. The results highlight the potential of this device for systematic study of MDM and cultivation of previously unculturable microorganisms.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Editorial Material
Cell Biology
Dale P. Corkery, Yao-Wen Wu
Summary: Extracellular pathogens utilize secreted virulence factors to regulate host cell function. Recently, the molecular mechanism behind host macroautophagy/autophagy regulation by the Vibrio cholerae toxin MakA has been characterized. Cholesterol binding at the plasma membrane induces MakA endocytosis and pH-dependent pore assembly. Membrane perforation of late endosomal membranes induces cellular membrane repair pathways and V-ATPase-dependent unconventional LC3 lipidation on damaged membranes.
Article
Biochemistry & Molecular Biology
Mazen Aseeri, Jose Luis Abad, Antonio Delgado, Gemma Fabrias, Gemma Triola, Josefina Casas
Summary: Ceramide plays a crucial role in regulating cellular senescence and apoptosis. Acid ceramidase (AC) reduces Ceramide levels, which is abnormally expressed in various cancers. However, the lack of suitable screening formats has hindered AC inhibitor discovery. In this study, a fluorogenic assay was adapted to a 384-well plate format, overcoming this limitation. The optimized platform successfully screened a library of 4100 compounds and identified several hits with potency ranges of 2-25 μM, belonging to different chemical classes. These inhibitors showed selectivity for neutral ceramidase and retained activity in cells, serving as a basis for further chemical optimization.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Engineering, Biomedical
Bingquan Yang, Klavdiya Gordiyenko, Andreas Schaefer, Seyed Mohammad Mahdi Dadfar, Wenwu Yang, Kristina Riehemann, Ravi Kumar, Christof M. Niemeyer, Michael Hirtz
Summary: In this study, three different surface coatings were compared to investigate their influence on DNA hybridization behavior. The structures and chemical properties of the coatings were characterized using various methods. The results showed that one of the coatings (PBAG) exhibited the highest hybridization efficiency, which has significant implications for the fabrication of DNA sensors.
ADVANCED NANOBIOMED RESEARCH
(2023)
Article
Nanoscience & Nanotechnology
Enrico Domenico Lemma, Roberta Tabone, Kai Richler, Ann-Kathrin Schneider, Claudia Bizzarri, Franco Weth, Christof M. Niemeyer, Martin Bastmeyer
Summary: Three-dimensional microscaffolds have shown great potential in mimicking physiological environments and simulating complex multicellular constructs in cell biology. However, controlling the precise localization of cells on microfabricated structures is still complex and limited to two-dimensional assays. This study presents a novel method using two-photon lithography and UV-mediated click reactions to selectively target different cell types to specific regions of a 3D microscaffold through DNA hybridization, enabling controlled cocultures of eukaryotic cells in 3D.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Leonie Schneider, Kersten S. Rabe, Carmen M. Dominguez, Christof M. Niemeyer
Summary: The immunological response of mast cells is regulated by the binding of antigens to IgE antibodies bound to the Fc epsilon RI receptor on the cell membrane surface. However, the organization and structural requirements of these antigen-antibody-receptor complexes at the nanometer scale are not fully understood. In this study, DNA nanostructures were used to generate multivalent artificial antigens, allowing for control over valency and nanoscale architecture. The results showed that the assembly of antibody-receptor complexes is critical for mast cell activation, and the affinity and nanoscale distance between the binding partners have less influence on the activation process.
Article
Biochemical Research Methods
Yao-Wen Wu
Summary: New red-green optogenetic dimerizers allow precise control of gene expression activation and deactivation with high spatiotemporal resolution.
Article
Biology
Lisa Goebel, Tonia Kirschner, Sandra Koska, Amrita Rai, Petra Janning, Stefano Maffini, Helge Vatheuer, Paul Czodrowski, Roger S. Goody, Matthias P. Mueller, Daniel Rauh
Summary: In this study, we developed nucleotide-based covalent inhibitors for the oncogenic mutant KRasG13C, which has been difficult to target in the past. These inhibitors showed promising properties and successfully locked KRasG13C in a crystal structure. Importantly, the modified KRasG13C protein with these inhibitors was unable to undergo nucleotide exchange and induce oncogenic signaling in cells, suggesting the potential use of nucleotide-based inhibitors for KRasG13C-driven cancer.
Article
Biotechnology & Applied Microbiology
Dong Liu, Yingying Liu, Hua-Zhen Duan, Xinjie Chen, Yanan Wang, Ting Wang, Qing Yu, Yong-Xiang Chen, Yuan Lu
Summary: This study customized the cell-free protein synthesis machinery and successfully synthesized phosphoproteins with site-specific phosphorylated amino acids, laying the foundation for developing a highly expansible CFPS platform and streamlining the production of user-defined phosphoproteins.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Editorial Material
Cell Biology
Dale P. Corkery, Yao-Wen Wu
Summary: ATG16L1 and TECPR1 are crucial components in the autophagy process. ATG16L1 provides membrane targeting for the ATG12-ATG5 conjugate, while TECPR1 functions independently and uses sphingomyelin for membrane targeting. Both proteins are involved in maintaining cellular autophagy and lysosomal repair.
Article
Biochemistry & Molecular Biology
Dale P. Corkery, Sergio Castro-Gonzalez, Anastasia Knyazeva, Laura K. Herzog, Yao-Wen Wu
Summary: This study reveals a novel role for TECPR1 in lysosomal membrane repair. TECPR1 is recruited to damaged membranes and forms a complex to regulate LC3 modification. The impairment of LC3 modification due to the double knockout of ATG16L1 and TECPR1 hinders lysosomal recovery.
Article
Chemistry, Organic
Sarah Zinken, Axel Pahl, Michael Grigalunas, Herbert Waldmann
Summary: Chromaline pseudo-natural products are a collection of topologically diverse compounds that were designed to access novel biological space by combining chromane and 4H-quinoline natural product fragments. The compounds possess both drug-like and natural product-like properties and have distinct bioactivity profiles compared to previous pseudo-natural product collections. This suggests that the new fragment combination may have led to a unique bioactivity.
Article
Chemistry, Multidisciplinary
Leonie Schneider, Kersten S. Rabe, Carmen M. Dominguez, Christof M. Niemeyer
Summary: This study utilizes DNA nanostructures to create multivalent artificial antigens and investigates the spatial requirements and critical factors for mast cell activation. It reveals the importance of antigen-directed assembly of antibody-receptor complexes in triggering degranulation.
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
