Article
Chemistry, Medicinal
Kazi Morshed Alom, Guralamatta Siddappa Ravi Kumara, Young Jun Seo
Summary: In this study, a fluorescent DNA probe containing an unnatural nucleotide was developed for the detection of alkaline phosphatase (ALP). The probe could bind to graphene oxide (GO) and quench the fluorescence in the absence of ALP or retain its fluorescence in the presence of ALP. By incorporating multiple unnatural nucleotides during primer extension, highly fluorescent DNA strands were obtained. This rkDNA-GO probing system showed high fluorescence differentiation for discriminating the status of ALP.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Analytical
Xiaowei Mu, Xue Jiang, Yu Zhang, Xin Liu, Siqi Zhang, Wei Wang, Yibing Huang, Pinyi Ma, Daqian Song
Summary: The study introduces a novel fluorescent assay for detecting alkaline phosphatase using chitosan carbon dots and calcein, showing excellent applicability in HepG2 cells and human serum samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Yan Wang, Ying Yan, Xinfa Liu, Changbei Ma
Summary: This study developed a simple and fast method to detect alkaline phosphatase quantitatively, with a LOD of 0.0098 U/L. The turn-off strategy allows for the detection of alkaline phosphatase presence, making it a potential tool for quantitative detection in clinical diagnosis.
Article
Chemistry, Analytical
Haiyan Li, Shangqing Zhang, Mingli Chen, Jianhua Wang
Summary: Bimetallic dual-ligands terbium nanocomplexes with unique internal structure were fabricated through in situ coordination assembly. By regulating the proportion of internal ligand SSA, the nanocomplexes showed excellent sensing specificity for detecting phosphate and pyrophosphate, with low detection limits.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Multidisciplinary
Meng Li, Bhaskar Gurram, Shan Lei, Nicholas T. Blum, Peng Huang, Jing Lin
Summary: Phosphatase is crucial in many biological functions, with alkaline phosphatase (ALP) serving as a key prognostic indicator for various diseases, though current detection methods are limited to in vitro colorimetric techniques. Fluorescence imaging provides a reliable way to monitor real-time changes in ALP levels, with research focusing mainly on ALP imaging probes grouped by their luminescence mechanisms. Challenges and future prospects for phosphatase probes were also discussed.
CHINESE CHEMICAL LETTERS
(2021)
Article
Materials Science, Multidisciplinary
Shun Wang, Beibei Han, Mengshuo Chen, Yaqing Han, Jifa Liu, Guannan Wang
Summary: This study synthesized bifunctional carbon dots with peroxidase-like and fluorescence properties, and developed a multi-signal sensing system for the detection of alkaline phosphatase (ALP). The detection limits and linear ranges of ALP were determined using colorimetric and fluorescent methods. The results showed that the strategy developed in this study has great potential for designing biosensors with bifunctional nanomaterials.
MATERIALS & DESIGN
(2023)
Article
Spectroscopy
Xueling Shang, Ying Yan, Jian Li, Xi Zhou, Xinying Xiang, Rong Huang, Xisheng Li, Changbei Ma, Xinmin Nie
Summary: As a representative biochemical indicator, alkaline phosphatase (ALP) plays a significant role in indicating and diagnosing clinical diseases. In this study, a signal-on fluorescence sensing method based on the enzyme-assisted target recycling (EATR) technique was developed for sensitive ALP activity detection. The method utilizes a two-step signal amplification process and demonstrates a sensitive limit of detection (LOD) and a linear range for quantifying ALP.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Food Science & Technology
Shixiang Wu, Xingping Zhang, Wang Chen, Guohao Zhang, Qian Zhang, Hualin Yang, Yu Zhou
Summary: Inspired by the strong binding capacity of pyrophosphate (ppi) and copper ion (Cu2+), a ratiometric fluorescence immunoassay triggered by alkaline phosphatase (ALP) was developed for the detection of zearalenone (ZEN). This method exhibited high accuracy and reliability, with a limit of detection (LOD) of 0.014 ng/mL and a linear detection range of 3.51-17.78 ng/mL. It provides a new approach for efficient and accurate detection of ZEN.
Article
Chemistry, Analytical
Liu Huang, Xiaozheng Cao, Tang Gao, Bin Feng, Xueyan Huang, Rong Song, Tao Du, Shijun Wen, Xueping Feng, Wenbin Zeng
Summary: A fluorescent probe THP consisting of AIE and ESIPT was developed for highly selective and sensitive detection of ALP activity, showing potential for biomedical applications in monitoring ALP activity in living cells.
Article
Chemistry, Analytical
Yingying Guo, Tianlu Zhao, Qiaonan Guo, Mingji Ding, Xiangrong Chen, Jianqing Lin
Summary: An upconversion signal readout platform for the detection of ALP was constructed using the IFE and the cascade signal amplification strategy. The method involved the oxidation of TMB by Ag+ to quench the UCL of UCNPs, which was then reversed by the catalytic hydrolysis of AAP by ALP. The developed method showed a linear correlation between ALP concentration and UCL recovery, and was successfully applied to detect ALP in serum samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Xuhua Zhao, Suya Zhao, Zhi-Ling Song, Xuanhao Zhang, Shi Zhang, Wenjuan Song, Zhuo Chen
Summary: We developed a SERS nanoprobe based on graphene-isolated-gold nanocrystals (AGIAN), which can achieve ratiometric analysis of alkaline phosphatase using acetonitrile as an internal standard, showcasing unique stability and reliable detection capabilities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Yu-An Feng, Hai Xu, Yang Zhou, Bao-Jun Wang, Jian Xiao, Ya-Wen Wang, Yu Peng
Summary: The newly designed NIR fluorescent probe SWJT-3 is capable of ratiometric determination of alkaline phosphatase (ALP) and can be used for both in vitro and in vivo detection. It is the first NIR and ratiometric fluorescent probe for imaging ALP in mice, with a large Stokes shift and low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xiao-Xue Jiang, Pan Li, Meng-Ya Zhao, Rui-Cong Chen, Zhen-Guang Wang, Jia-Xiu Xie, Yun-Kai Lv
Summary: Sulfur quantum dots (SQDs) were successfully encapsulated into metal-organic frameworks (ZIF-8) using a surface bound zinc ion-induced growth strategy, resulting in SQDs@ZIF-8 for alkaline phosphatase (ALP) detection. This new synthesis procedure significantly improved the luminous efficiency of SQDs. The detection of ALP was achieved by pH-triggered fluorescence quenching of SQDs@ZIF-8, and an extended detection range was obtained by complexing free SQDs with Fe2+. SQDs@ZIF-8 exhibited excellent stability in water and showed potential for fluorescence and colorimetric detection of ALP in human serum.
ANALYTICA CHIMICA ACTA
(2022)
Article
Spectroscopy
Xiaoqian Huang, Xiangzhu Chen, Shijun Chen, Xueyan Zhang, Lin Wang, Shicong Hou, Xiaodong Ma
Summary: A novel ratiometric fluorescent probe has been developed for the detection of alkaline phosphatase (ALP) and fluorescence imaging in living cells, showing high accuracy and sensitivity. The probe exhibits a significant fluorescence color change from blue to green in response to ALP, with a detection limit as low as 0.16 U L-1. Additionally, the probe demonstrates excellent biocompatibility for application in biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Applied
Ying-zhuo Shen, Zheng Wang, Kangping Ning, Chuanli Ren, Dandan Yang, Xiao-Ya Hu, Qin Xu
Summary: A highly sensitive and selective split-type perovskite-based photoelectrochemical (PEC) platform was developed to measure alkaline phosphatase (ALP) activity in milk and serum samples. The PEC used a CTAB@CH3NH3PbI3 film as the cathode to monitor the controlled AA production. With excellent sensitivity and selectivity, the platform has great potential for use in food and biomedical analysis.
Article
Automation & Control Systems
Haifei Peng, Jian Long, Cheng Huang, Shibo Wei, Zhencheng Ye
Summary: This paper proposes a novel multi-modal hybrid modeling strategy (GMVAE-STA) that can effectively extract deep multi-modal representations and complex spatial and temporal relationships, and applies it to industrial process prediction.
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
(2024)
