Article
Microbiology
Simon Blaine-Sauer, Myeong-Kyun Shin, Kristina A. Matkowskyj, Ella Ward-Shaw, Paul F. Lambert
Summary: This study demonstrates that MmuPV1 infection alone can efficiently mediate high-grade squamous intraepithelial lesions in the anal tract of mice using the NSG immunocompromised strain. We also show that MmuPV1, in combination with the chemical carcinogen DMBA, has carcinogenic potential. Furthermore, MmuPV1 is able to persist for up to 6 months in the anal tract of UVB-irradiated FVB/NJ mice and contributes to high-grade disease and cancer in an immunocompetent strain.
Article
Biology
Vural Yilmaz, Panayiota Louca, Louiza Potamiti, Mihalis Panayiotidis, Katerina Strati
Summary: This study successfully generated an in vivo lineage-tracing model to track papillomavirus-harboring cells and their progeny. By observing MmuPV1-treated tissues, increased proliferation dynamics and decreased MHC-I cell surface expression were found, which may have implications in tissue regenerative capacity and virus clearance. This model provides a novel tool to study the biology of MmuPV1 host-pathogen interactions.
Article
Medicine, Research & Experimental
Ziva Modic, Maja Cemazar, Bostjan Markelc, Andrej Cor, Gregor Sersa, Simona Kranjc Brezar, Tanja Jesenko
Summary: This study successfully developed a transplantable immunocompetent mouse model of HPV-positive OSCC and characterized it in vitro and in vivo. The results showed that this model exhibited increased radiosensitivity compared to traditional OSCC mouse models and can be used to explore immune-based treatment approaches.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, General & Internal
Giulia Ciccarese, Astrid Herzum, Gaetano Serviddio, Corrado Occella, Aurora Parodi, Francesco Drago
Summary: HPV vaccines may have a therapeutic role in addition to standard treatments for anogenital warts and oral warts, especially for persistent lesions.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Public, Environmental & Occupational Health
Zhen-Tong Wei, Hong-Liang Chen, Chun-Feng Wang, Gui-Lian Yang, Shu-Mei Han, Song-Ling Zhang
Summary: The study found that microbial perturbations occurred in the early phase of high-risk HPV infection, with a decrease in Lactobacillus and Sporolactobacillus, and an increase in bacteria related to bacterial vaginosis. These results provide insights into the correlation between high-risk HPV and the vaginal microbiota, suggesting that the predominance of some BV-associated bacteria during high-risk HPV infection may increase the risk for cervical neoplasia.
FRONTIERS IN PUBLIC HEALTH
(2021)
Review
Gastroenterology & Hepatology
Andreia Albuquerque, Mayura Nathan, Carmelina Cappello, Mario Dinis-Ribeiro
Summary: Anal squamous cell carcinoma, associated with anal HPV infection, is increasing in incidence and common in specific population groups. Screening aims to detect precancerous lesions for prevention, with various treatment options available for high-grade lesions. Gastroenterologists should be aware of these conditions for early detection in high-risk patients.
LANCET GASTROENTEROLOGY & HEPATOLOGY
(2021)
Article
Dermatology
Justin D. Arnold, Morgan E. Byrne, Anne K. Monroe, Stephen E. Abbott
Summary: This study found that adults living with HIV with a history of anogenital warts are at a higher risk of developing anal carcinoma. Clinicians should provide risk counseling for individuals in this population.
Article
Immunology
Chelsea Sanders, Rebecca L. Matthews, Sayyed Hamid Zarkesh Esfahani, Nazneen Khan, Nimit L. Patel, Joseph D. Kalen, Reinhard Kirnbauer, Richard B. Roden, Simone Difilippantonio, Ligia A. Pinto, Robert H. Shoemaker, Jason D. Marshall
Summary: This study expanded the application of the HPV pseudovirus (PsV) challenge model to include oral and vaginal inoculation, and demonstrated its effectiveness in testing vaccine-mediated dual-site immune protection against various HPV PsV types. Passive transfer of sera from mice vaccinated with the experimental HPV vaccine RG1-VLPs conferred HPV16-neutralizing and cross-neutralizing antibodies against HPV39 in naive recipient mice. Active vaccination with RG1-VLPs also provided protection against challenge with HPV16 or HPV39 PsVs at both vaginal and oral sites. These findings support the use of the HPV PsV challenge model for testing diverse HPV types at two sites associated with the most common HPV-associated cancers.
Article
Multidisciplinary Sciences
Basile Pache, Vincent Balaya, Jerome Mathis, Martin Hubner, Roland Sahli, Mathias Cavassini, Christine Sempoux, Patrice Mathevet, Martine Jacot-Guillarmod
Summary: This study aims to investigate the differences in risk factors for anal HR-HPV and dysplasia among women with or without cervical dysplasia or HIV-positive. The findings are expected to improve the screening standards for cervical and anal dysplasia in women by providing evidence of the presence of HPV and/or dysplasia in both the anus and cervix.
Review
Immunology
Feixue Wei, Catharina J. Alberts, Andreia Albuquerque, Gary M. Clifford
Summary: A review and meta-analysis showed that the HPV vaccine is highly effective against anal HPV infection and AIN in individuals under 26 years old who are HIV-negative. However, the effectiveness of the vaccine is lower in individuals over 26 years old who are living with HIV.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Shufa Liu, Yuanyue Li, Yuzhu Song, Xiaomei Wu, Zulqarnain Baloch, Xueshan Xia
Summary: This study aimed to explore the association between HPV infection and vaginal microbiota. The results showed that women with single HPV genotype infection had higher microbial diversity compared to HPV-negative and multiple HPV-positive women. The composition of vaginal microbiota varied among different HPV genotype infections, and the abundance of certain microbes changed compared to the control group.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Noluthando Mazibuko-Motau, Parveen Sobia, Jiawu Xu, Joseph Ahmed Elsherbini, James E. San, Lara Lewis, Andile Mtshali, Gugulethu Mzobe, Lungelo Ntuli, Salim S. Abdool Karim, Leila E. Mansoor, Quarraisha Abdool Karim, Douglas S. Kwon, Derseree Archary, Sinaye Ngcapu
Summary: This study investigated the impact of daily oral tenofovir disoproxil fumarate in combination with emtricitabine for PrEP on the vaginal microbiota in South African women. The results showed that the composition of vaginal microbiota was not significantly affected by PrEP, but the L. crispatus-dominant type had a higher probability of remaining stable in the PrEP group.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Neil D. Christensen, Kun-Ming Chen, Jiafen Hu, Douglas B. Stairs, Yuan-Wan Sun, Cesar Aliaga, Karla K. Balogh, Hannah Atkins, Debra Shearer, Jingwei Li, Sarah A. Brendle, Krishne Gowda, Shantu Amin, Vonn Walter, Raphael Viscidi, Karam El-Bayoumy
Summary: The study found that mice infected with MmuPV1 and treated with the tobacco carcinogen DBP have a higher risk of developing squamous cell carcinoma in oral tissues, compared to mice treated with virus or carcinogen individually. Virally-infected epithelium exhibited strong levels of viral DNA/RNA and viral protein staining, while areas of SCC showed reduced viral DNA staining but strong RNA signals.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Integrative & Complementary Medicine
Yan Xia, Ying Feng, Tianhua Qin, Xiaohua Zhao, Jing Lu, Cailing Ma
Summary: The study investigated the characteristics of vaginal microbiome in reproductive-age females with HPV infection in Xinjiang, China. The results showed that HPV infection alters the vaginal microbiota structure, and the changes in the proportions of Gardnerella and Shuttleworthia are associated with the severity of HPV infection.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Oncology
Annika Antonsson, Marjorie M. A. de Souza, Benedict J. Panizza, David C. Whiteman
Summary: Oropharyngeal cancer is increasingly caused by HPV, possibly due to changing sexual behavior. Sexual intercourse elicits a stronger immune response to HPV compared to oral sex, potentially leading to a weaker immune response in individuals who have their oral sex debut before or at the same time as sexual intercourse, increasing their risk of developing persistent oral HPV infection and oropharyngeal cancer.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Virology
Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Timothy K. Coope, Jiafen Hu, Neil D. Christensen
Review
Virology
Neil D. Christensen, Lynn R. Budgeon, Nancy M. Cladel, Jiafen Hu
Review
Virology
Jiafen Hu, Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Neil D. Christensen
Article
Virology
Nancy M. Cladel, Lynn R. Budgeon, Timothy K. Cooper, Karla K. Balogh, Jiafen Hu, Neil D. Christensen
JOURNAL OF VIROLOGY
(2013)
Article
Multidisciplinary Sciences
Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Timothy K. Cooper, Jiafen Hu, Neil D. Christensen
Review
Biology
Nancy M. Cladel, Xuwen Peng, Neil Christensen, Jiafen Hu
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2019)
Review
Oncology
Karam El-Bayoumy, Neil D. Christensen, Jiafen Hu, Raphael Viscidi, Douglas B. Stairs, Vonn Walter, Kun-Ming Chen, Yuan-Wan Sun, Joshua E. Muscat, John P. Richie
CANCER PREVENTION RESEARCH
(2020)
Article
Virology
Sarah Brendle, Jingwei J. Li, Nancy M. Cladel, Debra A. Shearer, Lynn R. Budgeon, Karla K. Balogh, Hannah Atkins, Marina Costa-Fujishima, Paul Lopez, Neil D. Christensen, John Doorbar, Thomas T. Murooka, Jiafen Hu
Summary: In this study using a mouse papillomavirus infection model, the impact of L1 and L2 on viral life cycle and tumor growth was investigated. The results showed that L1 is not essential for tumor growth induced by MmuPV1, and the L2 gene influences the expression and localization of the L1 protein in vivo.
Article
Virology
Samantha R. Hartmann, Daniel J. Goetschius, Jiafen Hu, Joshua J. Graff, Carol M. Bator, Neil D. Christensen, Susan L. Hafenstein
Summary: HPV is a significant health burden and a leading cause of virus-induced cancers, but studies have been hindered by restricted tropism. Alternative methods such as VLPs have been developed to overcome this issue, but structural studies have been limited by heterogeneity, fragility, and stability. By studying MmuPV1, a framework for continuing biochemical, genetic, and biophysical research for papillomaviruses has been provided.
Article
Immunology
Sarah Brendle, Nancy Cladel, Karla Balogh, Samina Alam, Neil Christensen, Craig Meyers, Jiafen Hu
Summary: The study indicates that CRPV and HPV58 capsids are more efficient in delivering external DNA compared to HPV16 capsids, especially at a capsid/DNA ratio of 2:1. Additionally, VLPs have been shown to increase in vivo infectivity of CRPV DNA in rabbits.
Article
Virology
Jiafen Hu, Sarah A. Brendle, Jingwei J. Li, Vonn Walter, Nancy M. Cladel, Timothy Cooper, Debra A. Shearer, Karla K. Balogh, Neil D. Christensen
Summary: Depo-medroxyprogesterone (DMPA) increases susceptibility of the anogenital tract to viral infection, but chronic administration does not promote cancer development in infected tissues.
Article
Virology
Pola Olczak, Ken Matsui, Margaret Wong, Jade Alvarez, Paul Lambert, Neil D. Christensen, Jiafen Hu, Bettina Huber, Reinhard Kirnbauer, Joshua W. Wang, Richard B. S. Roden
Summary: Licensed preventive HPV vaccines primarily target alpha HPVs but not beta HPVs. This study developed a two-component vaccine that can protect against both alpha and beta HPVs.
JOURNAL OF VIROLOGY
(2022)
Review
Virology
Nancy M. Cladel, Jie Xu, Xuwen Peng, Pengfei Jiang, Neil D. Christensen, Zhi-Ming Zheng, Jiafen Hu
Summary: Approximately 5% of human cancers are caused by HPV infections. The CRPV model has played a crucial role in the development of vaccines and studying HPV carcinogenesis. Recent studies using RNA-seq analysis and gene-modified rabbit lines have advanced our understanding of HPV pathogenesis and can potentially lead to the development of novel therapeutic strategies.
Review
Immunology
Michael Kozak, Jiafen Hu
Summary: Vaccines are the most cost-efficient and reasonable way to combat virulent pathogens. Different vaccine platforms and adjuvants have been used successfully to induce immune responses and protection. This review discusses the historical overview of vaccine development considering vaccine platforms, adjuvants, and delivery routes, as well as the advantages and limitations of each choice in efficacy.