Article
Biochemistry & Molecular Biology
An-Jen Chiang, Chia-Jung Li, Kuan-Hao Tsui, Chung Chang, Yuan-chin Ivan Chang, Li-Wen Chen, Tsung-Hsien Chang, Jim Jinn-Chyuan Sheu
Summary: The study found that UBE2C is highly expressed in human cervical squamous cell carcinoma (CESC) tissues and is related to clinical characteristics. In vitro experiments demonstrated that overexpression and knockdown of UBE2C respectively enhanced and reduced proliferation of cervical cancer cells. In vivo experiments showed that UBE2C regulates the expression and activity of the mTOR/PI3K/AKT pathway.
Article
Virology
Jonathan Miller, Aleksandra Dakic, Megan Spurgeon, Francisco Saenz, Bhaskar Kallakury, Bo Zhao, Junran Zhang, Jian Zhu, Qin Ma, Ying Xu, Paul Lambert, Richard Schlegel, Anna T. Riegel, Xuefeng Liu
Summary: The high-risk human papillomaviruses (HPV-16, -18) encode E6 and E7 proteins that target various pathways in host cells, including the AIB1 gene. Upregulation of AIB1 is associated with cervical cancer development and may serve as a histological biomarker. AIB1 expression increases with the progression of cervical lesions, suggesting its importance in cervical cancer.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Oncology
Marta del Pino, Isabel Matas, Pilar Carrillo, Cristina Marti, Ariel Glickman, Nuria Carreras-Dieguez, Lorena Marimon, Adela Saco, Natalia Rakislova, Aureli Torne, Jaume Ordi
Summary: Women with anal HPV infection treated for HSIL/CIN2-3 should be re-tested for anal HPV after treatment. Those who have persisting anal HPV infection after treatment are at high risk of developing HSIL/AIN and may benefit from anal exploration. Additionally, women with anal HPV infection treated for HSIL/CIN might have a higher risk of recurrent cervical HPV infection.
Article
Medicine, Research & Experimental
Xiaodong Sun, Pan Fu, Lixia Xie, Senmao Chai, Qianqian Xu, Lian Zeng, Xuanbin Wang, Nan Jiang, Ming Sang
Summary: The study confirmed that Resveratrol inhibits HPV E6 mRNA, HPV E6 protein, and phosphorylated retinoblastoma protein (p-pRb1) levels, while increasing p53 protein levels in HeLa and Ca Ski cells, as well as in subcutaneous tumor tissue grown from HeLa cells.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Biology
Vural Yilmaz, Panayiota Louca, Louiza Potamiti, Mihalis Panayiotidis, Katerina Strati
Summary: This study successfully generated an in vivo lineage-tracing model to track papillomavirus-harboring cells and their progeny. By observing MmuPV1-treated tissues, increased proliferation dynamics and decreased MHC-I cell surface expression were found, which may have implications in tissue regenerative capacity and virus clearance. This model provides a novel tool to study the biology of MmuPV1 host-pathogen interactions.
Article
Oncology
Chiara Herzog, Charlotte D. D. Vavourakis, James E. E. Barrett, Gerlinde Karbon, Andreas Villunger, Jiangrong Wang, Karin Sundstrom, Joakim Dillner, Martin Widschwendter
Summary: This study investigated the changes in the host epigenome induced by HPV infection and found that the WID-HPV can reflect the changes in the healthy host epigenome related to high-risk HPV infection. Interestingly, an increased WID-HPV index was observed in patients with minor cytological alterations, suggesting a successful viral clearance response. However, this response was not observed in patients with precancerous changes or invasive cervical cancer. Further analysis revealed that the WID-HPV is associated with apoptosis and the epigenetic replicative age.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Multidisciplinary Sciences
Xiaodong Sun, Hongxia Xu, Tianyu Dai, Lixia Xie, Qiang Zhao, Xincai Hao, Yan Sun, Xuanbin Wang, Nan Jiang, Ming Sang
Summary: This study highlights the significant role of BMI1 in cervical cancer, with high expression associated with poor differentiation, high clinical grade, and poor prognosis. Furthermore, alantolactone shows promising effects in reducing proliferation, migration, and invasion of cervical cancer cells, as well as promoting autophagy and mitochondrial damage.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Simon Blaine-Sauer, Myeong-Kyun Shin, Kristina A. Matkowskyj, Ella Ward-Shaw, Paul F. Lambert
Summary: This study demonstrates that MmuPV1 infection alone can efficiently mediate high-grade squamous intraepithelial lesions in the anal tract of mice using the NSG immunocompromised strain. We also show that MmuPV1, in combination with the chemical carcinogen DMBA, has carcinogenic potential. Furthermore, MmuPV1 is able to persist for up to 6 months in the anal tract of UVB-irradiated FVB/NJ mice and contributes to high-grade disease and cancer in an immunocompetent strain.
Article
Cell Biology
Qianhui Li, Bumin Xie, Xi Chen, Bingfeng Lu, Shuo Chen, Xiujie Sheng, Yang Zhao
Summary: Small nucleolar RNAs (snoRNAs) have been found to participate in the occurrence and development of malignant tumors. In this study, SNORD6 was identified as a snoRNA that affects the pathogenesis and prognosis of cervical cancer. Knockdown of SNORD6 inhibited cell proliferation and clone formation, induced G1-S phase cell cycle arrest, decreased DNA synthesis, reduced cell migration and invasion, and increased apoptosis. Mechanistically, SNORD6 promoted the formation of the tumor suppressor protein p53 degradation complex E6-E6AP-p53, leading to p53 ubiquitination and degradation, and influencing its regulation of cell cycle and apoptosis. This study elucidated the biological role and specific mechanism of SNORD6 in cervical cancer, contributing to the basic theoretical research and potential diagnosis and treatment strategies.
CELL DEATH DISCOVERY
(2023)
Article
Multidisciplinary Sciences
Liyuan Zhou, Qiongzi Qiu, Qing Zhou, Jianwei Li, Mengqian Yu, Kezhen Li, Lingling Xu, Xiaohui Ke, Haiming Xu, Bingjian Lu, Hui Wang, Weiguo Lu, Pengyuan Liu, Yan Lu
Summary: This study investigates the molecular mechanisms of cervical carcinogenesis caused by HPV DNA integration into the human genome. By analyzing 16 HPV16-positive cervical tumors, the authors identify distinct integration types, structural variations, and potential driver genes. The findings provide insights into the role and mechanism of HPV integration in cervical cancer.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Neil D. Christensen, Kun-Ming Chen, Jiafen Hu, Douglas B. Stairs, Yuan-Wan Sun, Cesar Aliaga, Karla K. Balogh, Hannah Atkins, Debra Shearer, Jingwei Li, Sarah A. Brendle, Krishne Gowda, Shantu Amin, Vonn Walter, Raphael Viscidi, Karam El-Bayoumy
Summary: The study found that mice infected with MmuPV1 and treated with the tobacco carcinogen DBP have a higher risk of developing squamous cell carcinoma in oral tissues, compared to mice treated with virus or carcinogen individually. Virally-infected epithelium exhibited strong levels of viral DNA/RNA and viral protein staining, while areas of SCC showed reduced viral DNA staining but strong RNA signals.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Review
Virology
Alexandre Santos Matos, Maria da Conceicao Viana Invencao, Ingrid Andressa de Moura, Antonio Carlos de Freitas, Marcus Vinicius de Aragao Batista
Summary: The human papillomavirus (HPV) is the most prevalent sexually transmitted infectious agent worldwide, causing cervical cancer by penetrating the epithelium through microlesions and establishing an infectious focus. In silico prediction tools can be used to select vaccine candidate T cell epitopes, based on their conservation within a group of antigenic proteins, enabling comprehensive genotypic coverage with a small set of epitopes. Therefore, this paper reviews the general characteristics of HPV biology and the current knowledge on developing therapeutic peptide vaccines against HPV-related infections and cervical cancer.
REVIEWS IN MEDICAL VIROLOGY
(2023)
Article
Virology
Yusha Chen, Suyu Li, Jinwen Zheng, Huifeng Xue, Jiancui Chen, Xiangqin Zheng
Summary: Multiple human papillomavirus (HPV) infections are common and controversial in their impact on cervical lesions. This study investigated the association between multiple HPV infections and cervical lesions, and found that single HR-HPV coinfected with LR-HPV may reduce the risk of high-grade cervical lesions.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Immunology
Shang-Ying Hu, Sabrina H. Tsang, Feng Chen, Qin-Jing Pan, Wen-Hua Zhang, Ying Hong, Joshua N. Sampson, Allan Hildesheim, Fang-Hui Zhao, Aimee R. Kreimer
Summary: The study found that vaginal infections are associated with incident non-HPV16/18 infections in the cervix, but do not impact the persistence of non-HPV16/18 infections after 6 months among women vaccinated with HPV16/18.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Timothee Dub, Sophie Le Coeur, Nicole Ngo-Giang-Huong, Wanmanee Matanasarawut, Pornnapa Suriyachai, Kannikar Saisawat, Chaiwat Putiyanun, Sudanee Buranabanjasatean, Prattana Leenasirimakul, Samreung Randaeng, Tristan Delory
Summary: This study found that among women living with HIV, treatment of cervical lesions may be beneficial for clearing HR-HPV infections. After treatment, the prevalence of HR-HPV significantly decreased.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Jiafen Hu, Lynn R. Budgeon, Nancy M. Cladel, Karla Balogh, Roland Myers, Timothy K. Cooper, Neil D. Christensen
JOURNAL OF GENERAL VIROLOGY
(2015)
Article
Virology
Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Timothy K. Coope, Jiafen Hu, Neil D. Christensen
Review
Virology
Neil D. Christensen, Lynn R. Budgeon, Nancy M. Cladel, Jiafen Hu
Review
Virology
Jiafen Hu, Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Neil D. Christensen
Article
Virology
Nancy M. Cladel, Lynn R. Budgeon, Timothy K. Cooper, Karla K. Balogh, Jiafen Hu, Neil D. Christensen
JOURNAL OF VIROLOGY
(2013)
Review
Biology
Nancy M. Cladel, Xuwen Peng, Neil Christensen, Jiafen Hu
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2019)
Review
Oncology
Karam El-Bayoumy, Neil D. Christensen, Jiafen Hu, Raphael Viscidi, Douglas B. Stairs, Vonn Walter, Kun-Ming Chen, Yuan-Wan Sun, Joshua E. Muscat, John P. Richie
CANCER PREVENTION RESEARCH
(2020)
Article
Virology
Sarah Brendle, Jingwei J. Li, Nancy M. Cladel, Debra A. Shearer, Lynn R. Budgeon, Karla K. Balogh, Hannah Atkins, Marina Costa-Fujishima, Paul Lopez, Neil D. Christensen, John Doorbar, Thomas T. Murooka, Jiafen Hu
Summary: In this study using a mouse papillomavirus infection model, the impact of L1 and L2 on viral life cycle and tumor growth was investigated. The results showed that L1 is not essential for tumor growth induced by MmuPV1, and the L2 gene influences the expression and localization of the L1 protein in vivo.
Article
Virology
Samantha R. Hartmann, Daniel J. Goetschius, Jiafen Hu, Joshua J. Graff, Carol M. Bator, Neil D. Christensen, Susan L. Hafenstein
Summary: HPV is a significant health burden and a leading cause of virus-induced cancers, but studies have been hindered by restricted tropism. Alternative methods such as VLPs have been developed to overcome this issue, but structural studies have been limited by heterogeneity, fragility, and stability. By studying MmuPV1, a framework for continuing biochemical, genetic, and biophysical research for papillomaviruses has been provided.
Article
Immunology
Sarah Brendle, Nancy Cladel, Karla Balogh, Samina Alam, Neil Christensen, Craig Meyers, Jiafen Hu
Summary: The study indicates that CRPV and HPV58 capsids are more efficient in delivering external DNA compared to HPV16 capsids, especially at a capsid/DNA ratio of 2:1. Additionally, VLPs have been shown to increase in vivo infectivity of CRPV DNA in rabbits.
Article
Virology
Jiafen Hu, Sarah A. Brendle, Jingwei J. Li, Vonn Walter, Nancy M. Cladel, Timothy Cooper, Debra A. Shearer, Karla K. Balogh, Neil D. Christensen
Summary: Depo-medroxyprogesterone (DMPA) increases susceptibility of the anogenital tract to viral infection, but chronic administration does not promote cancer development in infected tissues.
Article
Virology
Pola Olczak, Ken Matsui, Margaret Wong, Jade Alvarez, Paul Lambert, Neil D. Christensen, Jiafen Hu, Bettina Huber, Reinhard Kirnbauer, Joshua W. Wang, Richard B. S. Roden
Summary: Licensed preventive HPV vaccines primarily target alpha HPVs but not beta HPVs. This study developed a two-component vaccine that can protect against both alpha and beta HPVs.
JOURNAL OF VIROLOGY
(2022)
Review
Virology
Nancy M. Cladel, Jie Xu, Xuwen Peng, Pengfei Jiang, Neil D. Christensen, Zhi-Ming Zheng, Jiafen Hu
Summary: Approximately 5% of human cancers are caused by HPV infections. The CRPV model has played a crucial role in the development of vaccines and studying HPV carcinogenesis. Recent studies using RNA-seq analysis and gene-modified rabbit lines have advanced our understanding of HPV pathogenesis and can potentially lead to the development of novel therapeutic strategies.
Review
Immunology
Michael Kozak, Jiafen Hu
Summary: Vaccines are the most cost-efficient and reasonable way to combat virulent pathogens. Different vaccine platforms and adjuvants have been used successfully to induce immune responses and protection. This review discusses the historical overview of vaccine development considering vaccine platforms, adjuvants, and delivery routes, as well as the advantages and limitations of each choice in efficacy.