Review
Cell Biology
Chang Liu, Yuwen Chen, Yao Xie, Meixiang Xiang
Summary: This article discusses the role of tubulin post-translational modifications in regulating microtubule functions in the cardiac system, as well as the association between altered modifications and cardiac dysfunction, including heart failure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biology
Bruno Carmona, H. Susana Marinho, Catarina Lopes Matos, Sofia Nolasco, Helena Soares
Summary: Microtubules (MTs) are dynamic polymers of alpha/beta-tubulin heterodimers found in all eukaryotes, playing important roles in various cellular processes. Their functional diversity depends on the expression of distinct tubulin isotypes and post-translational modifications (PTMs). This review focuses on tubulin-acetylation, discussing its roles in MT stability, flexibility, and mechanical properties. It also explores the regulation of tubulin acetyltransferases/desacetylases and the association between MT acetylation and human pathologies.
Review
Biotechnology & Applied Microbiology
Onsurang Wattanathamsan, Varisa Pongrakhananon
Summary: Microtubules play a critical role in regulating cellular activities and their post-transitional modifications have been linked to cancer behavior. This review discusses the role of microtubule post-transitional modifications in cancer aggressiveness, particularly in cell death, chemotherapy sensitivity, cell migration, and invasion. Mechanistic pathways are also explained, providing valuable information for predictive and therapeutic purposes.
CANCER GENE THERAPY
(2023)
Review
Biochemistry & Molecular Biology
Silvia Requena, Francisco Sanchez-Madrid, Noa B. Martin-Cofreces
Summary: Tubulin post-translational modifications and MAPs contribute to the diversity of microtubule functions, regulating crucial cellular processes such as immune responses and viral infections.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Review
Cell Biology
Takashi Akera
Summary: Haploid gametes are generated from diploid parents via meiosis, a process essential for sexually reproducing eukaryotes. While the precise mechanism of faithful chromosome segregation in meiosis is not fully understood, it is increasingly evident that tubulin post-translational modifications (PTMs) play crucial roles in regulating microtubule functions during meiosis. This review highlights recent advances in understanding the roles of tubulin PTMs in meiotic spindles, particularly their involvement in spindle integrity, oocyte aging, and non-Mendelian inheritance.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Abhishek Ankur Balmik, Shweta Kishor Sonawane, Subashchandrabose Chinnathambi
Summary: The study shows that HDAC6's ZnF UBP domain can modulate Tau phosphorylation, actin cytoskeleton organization, neurite extension, and ApoE nuclear localization. Additionally, it plays a role in increasing tubulin localization in the microtubule organizing center. The ZnF domain of HDAC6 is involved in various regulatory functions in neurodegenerative diseases beyond its classical role in aggresome formation.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Agriculture, Multidisciplinary
Qibin Wu, Zhenxiang Li, Jingtao Yang, Fu Xu, Xueqin Fu, Liping Xu, Chuihuai You, Dongjiao Wang, Yachun Su, Youxiong Que
Summary: This study reports the first comprehensive analysis of protein lysine acetylation, 2-hydroxyisobutyrylation, and lysine lactylation in sugarcane. These post-translational modifications were found to be involved in energy metabolism and stress response. The results provide new insights into the molecular mechanisms of protein PTMs in sugarcane.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Cell Biology
Ewa Joachimiak, Dorota Wloga
Summary: Protists, a diverse group of single-celled eukaryotes, have different levels of sophistication in the organization of their microtubular cytoskeleton. The tubulin post-translational modifications (PTMs) in these organisms play a crucial role in building complex structures and have been studied using protists as model organisms. This article provides a brief summary of the current knowledge on tubulin PTMs in unicellular eukaryotes and highlights key findings in protists.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria Ovejero-Sanchez, Gloria Asensio-Juarez, Myriam Gonzalez, Pilar Puebla, Miguel Vicente-Manzanares, Rafael Pelaez, Rogelio Gonzalez-Sarmiento, Ana Belen Herrero
Summary: This study synthesized and characterized a new microtubule-destabilizing agent, PILA9, which showed strong cytotoxicity against OC cells. Additionally, the combination of microtubule-destabilizing agents with Panobinostat synergistically enhanced cytotoxicity, potentially through inducing alpha-tubulin acetylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jose Martinez-Hernandez, Julie Parato, Aditi Sharma, Jean-Marc Soleilhac, Xiaoyi Qu, Ellen Tein, Andrew Sproul, Annie Andrieux, Yves Goldberg, Marie-Jo Moutin, Francesca Bartolini, Leticia Peris
Summary: This study found an accumulation of tubulin acetylation in post-mortem brain tissues from Alzheimer's disease patients and human neurons with the Alzheimer's familial APP-V717I mutation. Impaired tubulin re-tyrosination may contribute to this acetylation accumulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Wenfeng Feng, Rong Liu, Xuan Xie, Lei Diao, Nannan Gao, Jinke Cheng, Xu Zhang, Yong Li, Lan Bao
Summary: The newly discovered SUMOylation on alpha-tubulin is an important posttranslational modification that affects microtubule properties and cellular functions. SUMOylation can promote microtubule catastrophe, interfere with its polymerization process, and inhibit the assembly of alpha-tubulin into microtubules.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Alexis Osseni, Aymeric Ravel-Chapuis, Edwige Belotti, Isabella Scionti, Yann-Gael Gangloff, Vincent Moncollin, Laetitia Mazelin, Remi Mounier, Pascal Leblanc, Bernard J. Jasmin, Laurent Schaeffer
Summary: Authors demonstrate that inhibiting HDAC6 and promoting Smad3 acetylation can reverse Duchenne muscular dystrophy-like symptoms in mdx mice, pointing to HDAC6 as a potential therapeutic target for the disorder.
NATURE COMMUNICATIONS
(2022)
Review
Neurosciences
Graziella Cappelletti, Alessandra Maria Calogero, Chiara Rolando
Summary: This study discusses the importance of microtubule acetylation in controlling neuronal and glial function, as well as its role in neurodegenerative disorders. Research has found that microtubule acetylation is a key modification that controls intracellular transport and stability, potentially serving as a novel therapeutic intervention for diseases like Parkinson's.
NEUROSCIENCE LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Karlie R. Platz, Emma J. Rudisel, Katelynn V. Paluch, Taylor R. Laurin, Kristin E. Dittenhafer-Reed
Summary: The mitochondrial proteome undergoes various post-translational modifications, and this study focused on the effects of acetylation and phosphorylation on the function of mitochondrial RNA polymerase (POLRMT). While some modifications showed slight decrease in the binding ability of POLRMT, there were minimal biological impacts observed in terms of viability, mtDNA content, and mitochondrial transcript levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Wei-hua Wang, Tao Yuan, Mei-jia Qian, Fang-jie Yan, Liu Yang, Qiao-jun He, Bo Yang, Jin-jian Lu, Hong Zhu
Summary: This review discusses the regulatory mode of post-translational modifications on KRAS, including prenylation, phosphorylation, and more, while highlighting recent studies targeting these modifications that have shown potent anti-tumor activities.
ACTA PHARMACOLOGICA SINICA
(2021)
Editorial Material
Cell Biology
Beatrice Benoit, Christian Pous
JOURNAL OF CELL BIOLOGY
(2019)
Article
Cell Biology
Benjamin Targa, Laurence Klipfel, Isabelle Cantaloube, Joelle Salameh, Beatrice Benoit, Christian Pous, Anita Baillet
CELL DEATH & DISEASE
(2019)
Article
Environmental Sciences
Daniel Perdiz, Lucie Oziol, Christian Pous
Article
Multidisciplinary Sciences
Valerie Campanacci, Agathe Urvoas, Soraya Cantos-Fernandes, Magali Aumont-Nicaise, Ana-Andreea Arteni, Christophe Velours, Marie Valerio-Lepiniec, Birgit Dreier, Andreas Pluckthun, Antoine Pilon, Christian Pous, Philippe Minard, Benoit Gigant
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Cell Biology
Geraldine Vilmen, Damien Glon, Gabriel Siracusano, Marion Lussignol, Zhouwulin Shao, Eva Hernandez, Daniel Perdiz, Frederique Quignon, Lina Mouna, Christian Pous, Henri Gruffat, Vincent Marechal, Audrey Esclatine
Summary: The BHRF1 protein encoded by Epstein-Barr virus inhibits IFNB induction by targeting mitochondria, stimulating mitochondrial fission and macroautophagy, leading to the formation of mito-aggresomes. This modulates the mitochondrial fate and counteracts innate immunity activation by promoting mitophagy.
Article
Cell Biology
Helene Henrie, Dalal Bakhos-Douaihy, Isabelle Cantaloube, Antoine Pilon, Maya Talantikite, Virginie Stoppin-Mellet, Anita Baillet, Christian Pous, Beatrice Benoit
JOURNAL OF CELL BIOLOGY
(2020)
Article
Endocrinology & Metabolism
Walid Haouari, Johanne Dubail, Samra Lounis-Ouaras, Pierre Prada, Rizk Bennani, Charles Roseau, Celine Huber, Alexandra Afenjar, Estelle Colin, Sandrine Vuillaumier-Barrot, Nathalie Seta, Francois Foulquier, Christian Pous, Valerie Cormier-Daire, Arnaud Bruneel
JOURNAL OF INHERITED METABOLIC DISEASE
(2020)
Article
Cell Biology
Beatrice Chambraud, Corentin Daguinot, Kevin Guillemeau, Melanie Genet, Omar Dounane, Geri Meduri, Christian Pous, Etienne Emile Baulieu, Julien Giustiniani
Summary: The decrease of FKBP4 in AD brain neurons may hinder autophagy efficiency, affecting MAPT secretion and accumulation.
Article
Optics
Pierre Jouchet, Clement Cabriel, Nicolas Bourg, Marion Bardou, Christian Pous, Emmanuel Fort, Sandrine Leveque-Fort
Summary: The modulated localization microscopy achieves high molecular localization precision through an illumination modulation method, improving axial localization precision while maintaining fast acquisition speed. Compared to existing super-resolution fluorescence microscopy, this method offers unique advantages.
Article
Biochemistry & Molecular Biology
Joelle Salameh, Isabelle Cantaloube, Beatrice Benoit, Christian Pous, Anita Baillet
Summary: This study demonstrates that cell resistance to taxanes involves the relocalization of septin from actin stress fibers to microtubules, which is triggered by acute paclitaxel treatment due to the release of Cdc42 effectors BORG2 and BORG3. Inhibiting Cdc42 leads to septin detachment from stress fibers and relocalization to microtubules, while overexpression of BORG2 or BORG3 retains septins on actin fibers even after paclitaxel treatment. These findings highlight the critical role of Cdc42, BORG2, and BORG3 in regulating the interplay between septins, actin fibers, and microtubules in response to taxanes.
Review
Biochemistry & Molecular Biology
Beatrice Benoit, Anita Baillet, Christian Pous
Summary: This review extensively covers the complex relationships between JNK and cytoskeleton elements, including mechanisms controlling JNK activation, known substrates, and the specific locations of upstream regulators. It also provides techniques and tools for further research on the interplay between different types of cytoskeleton filaments mediated by JNK.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Pierre Jouchet, Christian Pous, Emmanuel Fort, Sandrine Leveque-Fort
Summary: Structured illumination improves localization precision in single-molecule localization microscopy by providing new information on molecular positions. This study used time-shifted sinusoidal excitation and synchronous demodulation to modulate and recover the fluorescence signal. Flexible fast demodulation systems were designed to overcome camera acquisition frequency limitations. The ModLoc microscopy improved localization precision by a factor of 2.4 compared to classical Gaussian fitting methods.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Edith Renguet, Marine De Loof, Natacha Fourny, Audrey Ginion, Caroline Bouzin, Christian Pous, Sandrine Horman, Christophe Beauloye, Laurent Bultot, Luc Bertrand
Summary: Our study demonstrates that modulation of α-tubulin K40 acetylation level affects glucose transport in cardiomyocytes, offering new putative therapeutic insights regarding modulation of glucose metabolism in insulin-resistant and diabetic hearts.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mohyeddine Omrane, Amanda Souza Camara, Cyntia Taveneau, Nassima Benzoubir, Thibault Tubiana, Jinchao Yu, Raphael Guerois, Didier Samuel, Bruno Goud, Christian Pous, Stephane Bressanelli, Richard Charles Garratt, Abdou Rachid Thiam, Ama Gassama-Diagne
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)