Article
Biochemistry & Molecular Biology
Melissa Girard, Steve Dagenais Bellefeuille, Emilie Eiselt, Guillaume Arguin, Jean-Michel Longpre, Philippe Sarret, Fernand-Pierre Gendron
Summary: This study found that endosomal trafficking plays an important role in G protein-coupled receptor (GPCR) signaling. UDP selectively activates GPCR P2Y6 as a signaling molecule. The study found that MRS2693 and UDP have different effects on the internalization of the P2Y6 receptor, with MRS2693 inducing P2Y6 internalization through an independent caveolin-dependent mechanism. This study provides insights for the development of bias ligands that can influence P2Y6 signaling.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Review
Medicine, Research & Experimental
Juan Carlos Martinez-Morales, K. Helivier Solis, M. Teresa Romero-Avila, Guadalupe Reyes-Cruz, J. Adolfo Garcia-Sainz
Summary: G protein-coupled receptors (GPCRs) are membrane proteins that function as sensors and play significant roles in various physiological and pathological processes. This review provides an overview of the current understanding of the structure, signaling, internalization, and recycling of GPCRs.
ARCHIVES OF MEDICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Vincent B. Luscombe, Luis Alberto Baena-Lopez, Carole J. R. Bataille, Angela J. Russell, David R. Greaves
Summary: In this study, heterologous cell lines with low GPR84 expression levels were developed to mimic the response of primary cells in a label-free cell electrical impedance sensing system. It was found that DL-175 exhibited a delayed impedance response, a delayed and suppressed activation of Akt, and impaired ability to internalise GPR84 from the plasma membrane compared to 6-OAU. These signalling differences were transient and occurred only at early time points in the low expressing cell lines, highlighting the importance of receptor number and kinetic readouts when evaluating signalling bias. These findings provide new insights into GPR84 signalling and the evaluation of newly developed agonists.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Immunology
Yi Wang, Cheng-long Zhu, Peng Li, Qiang Liu, Hui-ru Li, Chang-meng Yu, Xiao-ming Deng, Jia-feng Wang
Summary: Sepsis is a life-threatening dysfunction caused by an uncontrolled host response to infection, leading to respiratory dysfunction and acute respiratory distress syndrome (ARDS). Neutrophils, the first line of defense against infection, play a major role in sepsis. However, studies have shown that despite high levels of chemokines at the site of infection, neutrophils cannot migrate properly and instead accumulate in the lungs, causing tissue damage and ARDS. Dysregulation of chemokine receptors, particularly G protein-coupled receptors (GPCRs), is implicated in impaired neutrophil migration. This review summarizes the signaling pathways and mechanisms by which GPCR dysfunction in sepsis leads to impaired neutrophil chemotaxis and proposes potential targets for intervention to improve neutrophil chemotaxis.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Endocrinology & Metabolism
Fanhua Wang, Mingyao Liu, Ning Wang, Jian Luo
Summary: This review discusses the role of G-protein coupled receptors (GPCRs) in osteoarthritis (OA), including the pathophysiological processes involved, preclinical and clinical trial data, and the challenges in developing therapies targeting GPCRs for OA.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yanan Tian, Chaohui Jiang, Yi Pan, Zhiqiang Guo, Weiwei Wang, Xumei Luo, Zheng Cao, Bing Zhang, Jingwen Yang, Ying Shi, Naiming Zhou, Xiaobai He
Summary: Two newly identified CCHamide receptors, BommoCCHaR-1 and -2, have been cloned and their specific endogenous ligands, CCHamide-1 and CCHamide-2, respectively, have been characterized. The receptors exhibit different signaling pathways upon activation, with BNGR-A14 eliciting increases in CRE-driven luciferase activity, intracellular Ca2+ mobilization, and ERK1/2 phosphorylation, while BNGR-A15 leads to intracellular accumulation of cAMP, Ca2+ mobilization, and ERK1/2 phosphorylation. Additionally, CCHamides are shown to require intrachain disulfide bonds for activation, and CCHamide-1 may regulate feeding behavior and growth through BNGR-A15, while CCHamide-2 plays a crucial role in multiple physiological processes.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Kate F. Byrne, Ajay Pal, James F. Curtin, John C. Stephens, Gemma K. Kinsella
Summary: The focus of the review is on G-protein-coupled receptor (GPCR) targets, with chemokine, cannabinoid, and dopamine receptors showing promise. Further research is needed on potential targets such as MC4R, adhesion receptors, LPA, and Smo receptors to develop new drug-screening strategies for safe and effective GBM therapies.
DRUG DISCOVERY TODAY
(2021)
Review
Biochemistry & Molecular Biology
Dekel David, Ziv Bentulila, Merav Tauber, Yair Ben-Chaim
Summary: GPCRs are involved in signal transduction processes, and although they span the cell membrane, they have not been considered to be regulated by membrane potential. Recent studies, however, have shown that several GPCRs are voltage regulated. This review discusses the advances in understanding the voltage dependence of GPCRs, the suggested molecular mechanisms, and the possible physiological roles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Ramon Cierco Jimenez, Nil Casajuana-Martin, Adrian Garcia-Recio, Lidia Alcantara, Leonardo Pardo, Mercedes Campillo, Angel Gonzalez
Summary: The study analyzed 119,069 natural variants in human olfactory receptors, revealing a significant diversity of natural variations in the olfactory gene repertoire between individuals and populations, with a considerable number of changes occurring at the structurally conserved regions. Mutations in positions linked to the conserved GPCR activation mechanism were highlighted, which could imply phenotypic variation in olfactory perception.
Review
Pharmacology & Pharmacy
Sergi Ferre, Francisco Ciruela, Carmen W. Dessauer, Javier Gonzalez-Maeso, Terence E. Hebert, Ralf Jockers, Diomedes E. Logothetis, Leonardo Pardo
Summary: The study proposes the concept of GPCR-effect assemblies (GEMMAs), which are pre-assembled before receptor activation and allow more efficient interactions between specific signaling components. This offers an alternative model to the conventional collision coupling model and explains the differential properties of GPCRs in different cellular environments.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Chemistry, Multidisciplinary
Xin-heng He, Chong-zhao You, Hua-liang Jiang, Yi Jiang, H. Eric Xu, Xi Cheng
Summary: G protein-coupled receptors (GPCRs) are important drug targets that play crucial roles in various physiological processes. Although extensive efforts have been made in the field of structural biology, a significant number of GPCR structures remain unsolved due to their structural instability. Recently, AlphaFold2 has been developed as a tool to predict the structure models of GPCRs and other functionally important proteins. However, our evaluation reveals several differences between the predicted models and experimental structures, such as the assembly of domains, shape of ligand-binding pockets, and conformation of binding interfaces. These differences hinder the use of predicted structure models in functional studies and structure-based drug design, where reliable high-resolution structural information is required.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Biochemistry & Molecular Biology
Wojciech Pietrus, Rafal Kurczab, Dagmar Stumpfe, Andrzej J. Bojarski, Juergen Bajorath
Summary: The study showed that introducing fluorine can significantly increase ligand potency, but the effect of fluorination on affinity varies depending on the fluorination position. Fluorination of the aromatic ring at the ortho position is favorable for potency enhancement, while fluorination of aliphatic fragments more often leads to a decrease in biological activity.
Article
Biochemical Research Methods
Jeffrey F. Diberto, Katie Smart, Reid H. J. Olsen, Bryan L. Roth
Summary: TRUPATH is a platform based on bioluminescence resonance energy transfer for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors. Protocols for agonist and antagonist TRUPATH assays in the 384-well plate format are provided, offering higher throughput capabilities.
Article
Chemistry, Multidisciplinary
Yunfang Xiong, Ran Ke, Qingyu Zhang, Wenjun Lan, Wanjun Yuan, Karol Nga Ieng Chan, Tom Roussel, Yifan Jiang, Jing Wu, Shuai Liu, Alice Sze Tsai Wong, Joong Sup Shim, Xuanjun Zhang, Ruiyu Xie, Nelson Dusetti, Juan Iovanna, Nagy Habib, Ling Peng, Leo Tsz On Lee
Summary: This study reports the effective modulation of a GPCR for cancer treatment using small activating RNAs (saRNAs) for the first time. The saRNAs promote the expression of MAS1, a GPCR that counteracts cancer cell proliferation and migration. By enhancing MAS1 expression, these saRNAs suppress tumorigenesis and inhibit tumor progression in multiple cancer models. This research not only provides a new strategy for cancer therapy by targeting the renin-angiotensin system, but also offers a new avenue to modulate GPCR signaling through RNA activation.
Review
Engineering, Biomedical
Yuhong Jiang, Yuke Li, Xiujuan Fu, Yue Wu, Rujing Wang, Mengnan Zhao, Canquan Mao, Sanjun Shi
Summary: The translation article introduces the interaction between G protein-coupled receptors (GPCRs) and nanotechnology, as well as how nanotechnology can improve the efficacy and safety of GPCR-related drugs. Nanotechnology can encapsulate GPCR ligands to construct synthetic nano-GPCRs and precisely initiate sustained endosomal signal transduction through nanoparticles. Moreover, nanoparticles can enhance the potency of delivery systems by actively targeting specific cells through ligand-receptor binding and receptor-dependent endocytosis.
ACTA BIOMATERIALIA
(2023)
Editorial Material
Chemistry, Multidisciplinary
Annette G. Beck-Sickinger, Neville Compton, Wolfram Koch, Theresa Kueckmann, Katharina Landfester, Frank Maass, Peter R. Schreiner, Xin Su, Suzanne Tobey, Nathalie Weickgenannt
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Sabrina Spiller, Franziska Clauder, Kathrin Bellmann-Sickert, Annette G. Beck-Sickinger
Summary: Implant design has shifted from biochemically inert to bioactive substrates, aiding tissue regeneration. Key aspects include controlling cell adhesion, distinguishing bacteria from desired tissue cells, and promoting implant integration and wound healing. Bioactive peptides are versatile in regulating surface, cell, and matrix interactions, enhancing therapeutic efficiency and reducing doses.
BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Lizzy Wanka, Victoria Behr, Annette G. Beck-Sickinger
Summary: The internalization of GPCRs is crucial for regulating signal strength and receptor activation opportunities, with a focus on rhodopsin-like GPCRs in agonist-induced, arrestin-dependent internalization processes. Understanding this mechanism can aid in drug development targeting GPCRs, with the neuropeptide Y receptor family representing an interesting target for novel therapeutics due to its involvement in various physiological and pathophysiological processes.
BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sarina Rudolf, Kerstin Kaempf, Oanh Vu, Jens Meiler, Annette G. Beck-Sickinger, Irene Coin
Summary: Through biochemical and computational approaches, we investigated the binding mode of natural peptide ligands to the Y5R receptor, mapping interactions and building a structural model based on experimental data.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemistry & Molecular Biology
Tobias F. Fischer, Annette G. Beck-Sickinger
Summary: Chemerin is a chemotactic protein that plays a key role in initiating the early immune response. It is also involved in energy homeostasis and the regulation of reproductive functions. Chemerin activates different receptors to exert its biological activity and has an impact on cancer and obesity-related diseases. Therapeutic targeting of the chemerin system is currently being explored.
BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Philipp Wolf, Alexander Mohr, Georgina Gavins, Victoria Behr, Karin Moerl, Oliver Seitz, Annette G. Beck-Sickinger
Summary: Fine-tuning of GPCR signaling is crucial for cellular homeostasis, and recent studies have shown that lateral GPCR interactions in the cell membrane can impact signaling profiles. A one-step labeling method for multiple membrane-embedded GPCRs based on short peptide tags has been successfully developed, providing novel insight into the regulation of GPCR signaling. The study also demonstrates the regulation of GPCR signaling by ETAR expression and the constitutive proximity between hetero-receptors limited to ETAR/ETBR.
Review
Biochemistry & Molecular Biology
Oanh Vu, Brian Joseph Bender, Lisa Pankewitz, Daniel Huster, Annette G. Beck-Sickinger, Jens Meiler
Summary: G protein-coupled receptors (GPCRs) are the largest membrane protein family and a significant target class for therapeutics. The class A receptors of GPCRs affect various aspects of human physiology. Many peptides that bind to these receptors undergo interaction and conformational changes.
Review
Transplantation
Carmine Zoccali, Alberto Ortiz, Inga Arune Blumbyte, Sarina Rudolf, Annette G. Beck-Sickinger, Jolanta Malyszko, Goce Spasovski, Sol Carriazo, Davide Viggiano, Justina Kurganaite, Vaiva Sarkeviciene, Daiva Rastenyte, Andreja Figurek, Merita Rroji, Christopher Mayer, Mustapha Arici, Gianvito Martino, Gioacchino Tedeschi, Annette Bruchfeld, Belinda Spoto, Ivan Rychlik, Andrzej Wiecek, Mark Okusa, Giuseppe Remuzzi, Francesca Mallamaci
Summary: NPY, a 36-amino-acid peptide, regulates various biological functions and plays a key role in chronic kidney disease progression, cardiovascular events, and cognitive decline. Interfering with the NPY system has potential for treating diverse diseases, although challenges in drug delivery, cost, and complexity of diseases remain.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Article
Multidisciplinary Sciences
Tingting Tang, Qiuxiang Tan, Shuo Han, Anne Diemar, Kristin Lobner, Hongyu Wang, Corinna Schuess, Victoria Behr, Karin Moerl, Mu Wang, Xiaojing Chu, Cuiying Yi, Max Keller, Jacob Kofoed, Steffen Reedtz-Runge, Anette Kaiser, Annette G. Beck-Sickinger, Qiang Zhao, Beili Wu
Summary: This study reports the structures of human Y-1, Y-2, and Y-4 receptors in complex with NPY or PP, and the G(i)(1) protein, revealing distinct binding poses of the peptide upon coupling to different receptors, as well as subtype-specific interactions between the receptors and peptides.
Article
Chemistry, Medicinal
Anne Sophie Czerniak, Kevin Kretschmer, Tina Weiss, Annette G. Beck-Sickinger
Summary: This study investigates the binding mode of the adipokine chemerin and its short peptide agonist C9 to their receptors CMKLR1 and GPR1 using the NanoBRET binding assay. The results show that CMKLR1 requires full-length chemerin for stable binding, while GPR1 exhibits high affinity and comparable binding for both ligands.
Article
Biochemistry & Molecular Biology
Kevin Kretschmer, Jan Stichel, Kathrin Bellmann-Sickert, Lars Baumann, Donald Bierer, Bernd Riedl, Annette G. Beck-Sickinger
Summary: Semaphorin-3A is involved in kidney development and acute kidney injury. The interaction between Sema-3A and its receptor NRP-1 can be modulated by peptide inhibitors. The study identified a specific binding site for a peptide inhibitor on Sema-3A, providing a potential target for developing therapeutic peptides to treat acute kidney injury.
JOURNAL OF PEPTIDE SCIENCE
(2023)
Article
Oncology
Annette G. Beck-Sickinger, Daniel P. Becker, Oksana Chepurna, Bhaskar Das, Sebastian Flieger, Evamarie Hey-Hawkins, Narayan Hosmane, Satish S. Jalisatgi, Hiroyuki Nakamura, Rameshwar Patil, Maria da Graca H. Vicente, Clara Vinas
Summary: This article summarizes current research on the development of boron delivery drugs for boron neutron capture therapy, which were presented and discussed at the National Cancer Institute (NCI) Workshop on Neutron Capture Therapy held on April 20-22, 2022. The most commonly used boron sources are icosahedral boron clusters attached to various molecules such as peptides, proteins, porphyrin derivatives, dendrimers, polymers, and nanoparticles, as well as encapsulated into liposomes. These boron clusters and/or carriers can be labeled with contrast agents, allowing for imaging techniques like PET, SPECT, and fluorescence to quantify tumor-localized boron and serve as theranostic agents.
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
(2023)
Article
Biochemistry & Molecular Biology
Kevin Kretschmer, Tristan Zellmann, Karin Moerl, Annette G. Beck-Sickinger
Summary: This study investigates the interaction between adipokine chemerin and its receptor, CMKLR1, and demonstrates that stable binding is mediated by negative charges in the receptor's N terminus and a specific positively charged patch on the surface of full-length chemerin. This finding provides insights into the mechanism of inflammation-related diseases and could aid in the development of more potent therapeutic ligands.
Article
Materials Science, Multidisciplinary
Anca Constantina Parau, Mihaela Dinu, Cosmin Mihai Cotrut, Iulian Pana, Diana Maria Vranceanu, Lidia Ruxandra Constantin, Giuseppe Serratore, Ioana Maria Marinescu, Catalin Vitelaru, Giuseppina Ambrogio, Dennis Alexander Boehner, Annette G. Beck-Sickinger, Alina Vladescu (Dragomir)
Summary: The effect of deposition temperature on the structure, morphology, hardness, electrochemical evaluation, degradation properties and functional peptides adhesion of Mg and Si-doped hydroxyapatite coatings was investigated. The results showed that an increase in deposition temperature led to an improvement in hardness and corrosion resistance. The degradation rate of the coatings was slowed at higher deposition temperature, especially in DMEM medium. Both deposition temperatures are suitable for further bio-inspired coating with a mussel-derived peptide, to facilitate biointegration.
Article
Biochemistry & Molecular Biology
Eva-Maria Juelke, Jan-Patrick Fischer, Sylvia Els-Heindl, Donald Bierer, Ingo Flamme, Johannes Koebberling, Bernd Riedl, Annette G. Beck-Sickinger
Summary: This study reports a method for developing ADM analogs with increased proteolytic stability and high receptor selectivity through rational modifications. The modifications include lactamization and lipidation, resulting in highly stabilized analogs with long-term in vivo activity. The analogs also display dose-dependent vasodilatory effects in rodents.
JOURNAL OF PEPTIDE SCIENCE
(2023)
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)