Article
Biotechnology & Applied Microbiology
Shaoqi Sun, Lin Shu, Xiyang Lu, Qinghui Wang, Marina Tisma, Chenguang Zhu, Jiping Shi, Frank Baganz, Gary J. Lye, Jian Hao
Summary: The study demonstrates an efficient way of producing 1,2-propanediol from glycerol via an endogenous pathway of K. pneumoniae. Key enzymes for 1,2-propanediol synthesis were identified, and optimal process parameters were determined for maximal production in the strain.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Immunology
Jinhui Tang, Liwen Gu, Jieyu Luo, Haihua Luo, Qingli Zeng, Yong Jiang
Summary: This study confirms the protective role of 1,25(OH)2D3 in macrophages against Klebsiella pneumoniae (Kp) infection and demonstrates its promotion of autophagy activity. Inhibiting autophagy impairs the ability of 1,25(OH)2D3 to eliminate Kp infection and its anti-inflammatory effects. These protective effects are validated in a mouse model and provide evidence for further exploration of 1,25(OH)2D3 as an adjunctive therapy for bacterial infections.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Immunology
Xiaodan Guan, Liang Jin, Daojun Yu, Yu He, Yida Bao, Huifen Zhou, Haitong Wan
Summary: This study found that glycyrrhetinic acid (GA) can protect pulmonary epithelial cells from CRKP-induced damage by regulating mitochondrial damage and antioxidative stress.
MICROBIAL PATHOGENESIS
(2023)
Article
Immunology
Fatemeh Asgari, Domenico Supino, Raffaella Parente, Nadia Polentarutti, Matteo Stravalaci, Remi Porte, Fabio Pasqualini, Marialuisa Barbagallo, Chiara Perucchini, Camilla Recordati, Elena Magrini, Andrea Mariancini, Federica Riva, Alessia Giordano, Sadaf Davoudian, Thierry Roger, Cornelis van't Veer, Sebastien Jaillon, Alberto Mantovani, Andrea Doni, Cecilia Garlanda
Summary: The study highlights the important role of PTX3 in controlling Klebsiella pneumoniae infection by modulating inflammatory responses and tissue damage.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Federico Serral, Agustin M. Pardo, Ezequiel Sosa, Maria Mercedes Palomino, Marisa F. Nicolas, Adrian G. Turjanski, Pablo Ivan P. Ramos, Dario Fernandez Do Porto
Summary: Carbapenem-resistant Klebsiella pneumoniae (CR-KP) is an emerging threat to public health, and the development of novel antimicrobials is urgently needed. This study used genome-wide interrogation and metabolic data integration to identify potential targets for drug development. Pathways involved in lipopolysaccharide and peptidoglycan synthesis were confirmed as suitable targets, while other cellular functions, such as energy metabolism pathways, were also suggested for further investigation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Xueting Wang, Chunxia Bi, Xiaoni Xin, Mengmeng Zhang, Hengxia Fu, Lei Lan, Mengyuan Wang, Zhiyong Yan
Summary: The study investigated the impact of Klebsiella pneumoniae infection on host cells, specifically on pyroptosis, apoptosis, and autophagy, to understand its pathogenic mechanism. The results revealed that hypervirulent K. pneumoniae showed higher resistance to phagocytosis, causing more severe cellular and lung tissue damage compared to classical K. pneumoniae. Hypervirulent K. pneumoniae also induced higher levels of pyroptosis, while classical K. pneumoniae strongly triggered autophagy. These findings provide new insights into the pathogenesis of K. pneumoniae and could guide the development of future treatments for K. pneumoniae infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daiane Boff, Remo Castro Russo, Helena Crijns, Vivian Louise Soares de Oliveira, Matheus Silverio Mattos, Pedro Elias Marques, Gustavo Batista Menezes, Angelica Thomaz Vieira, Mauro Martins Teixeira, Paul Proost, Flavio Almeida Amaral
Summary: This study tested the anti-inflammatory effect of CXCL9(74-103) in Klebsiella pneumoniae-induced pneumonia. The treatment with CXCL9(74-103) reduced neutrophil migration, decreased the production of IL-1 beta, and improved lung function in infected mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yizhe Zhang, Chaoyin Zhu, Hongjun Zhao, Zhanyang Sun, Xiaodi Wang
Summary: This study investigated the anti-inflammatory mechanism of chlorogenic acid (CGA) in rats with severe pneumonia induced by Klebsiella pneumoniae (Kp) infection. The results showed that CGA improved survival rates and alleviated pneumonia symptoms by upregulating the expression of microRNA-124-3p and inactivating the p38MAPK pathway to reduce inflammatory levels.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Immunology
Wei Jiang, Jun Liu, Xuequn Zhao, Wenjie Yang
Summary: This study investigated the mechanisms by which melatonin suppressed K. pneumoniae-induced inflammation and apoptosis in lung cells and found that melatonin inhibited inflammation and apoptosis through the AMPK pathway.
INFLAMMOPHARMACOLOGY
(2022)
Article
Biology
Lei Li, Jie Guan, Rongjun Lin, Fang Wang, Hui Ma, Chenggang Mao, Xingqing Guo, Zhenghai Qu, Renzheng Guan
Summary: This study aimed to investigate the effects of astragaloside IV, a biologically active substance derived from Astragalus mambranaceus Bunge, on Klebsiella pneumonia rats and the underlying mechanisms. It was found that astragaloside IV alleviated the inflammation caused by Klebsiella pneumonia through suppressing the TGF-01/Smad pathway and improved lung tissue damage.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Article
Microbiology
Philipp Grubwieser, Richard Hilbe, Clemens Michael Gehrer, Manuel Grander, Natascha Brigo, Alexander Hoffmann, Markus Seifert, Sylvia Berger, Igor Theurl, Manfred Nairz, Guenter Weiss
Summary: During infection, Klebsiella pneumoniae manipulates macrophage iron metabolism to acquire iron for sustained proliferation. This mechanism of microbe-mediated intracellular bacterial persistence provides insights for the development of novel antimicrobial therapies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hexin Liu, Peng Zhao, Pingfang Tian
Summary: In this study, the researchers engineered a CRISPRi system to convert glucose into glycerol and improve the production of 3-hydroxypropionic acid. The bi-functional strain with the engineered CRISPRi system showed an increase in glycerol and 3-HP production using glucose as the carbon source.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Xin Liu, Xinchuan Zheng, Yongling Lu, Qian Chen, Jiang Zheng, Hong Zhou
Summary: This review discusses the role of TFEB in regulating the ALP pathway and its protective effects in sepsis, including immune modulation and organ protection. It also summarizes the diverse pharmacological targets of TFEB activators and recent advances in their potential therapeutic applications for treating sepsis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Young-Sun Lee, Deepak Prasad Gupta, Sung Hee Park, Hyun-Jeong Yang, Gyun Jee Song
Summary: The study found that DMF can inhibit nitric oxide and proinflammatory cytokine production in microglia, induce microglial switching to the M2 state, increase the expression levels of autophagy markers such as LC3 and ATG7, and promote the formation of autophagosomes. These results suggest that DMF induces autophagy in microglia and its anti-inflammatory effects are partially mediated through an autophagy-dependent pathway.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Yahong Zheng, Yuting Ding, Mengran Xu, Haoran Chen, Hui Zhang, Yanyan Liu, Weihua Shen, Jiabin Li
Summary: The study identified the gut microbiota as a protective factor against K. pneumoniae infection, and found that FMT could improve inflammatory levels and liver damage in KLA patients, increasing survival rates.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Microbiology
Caisey V. Pulford, Blanca M. Perez-Sepulveda, Rocio Canals, Jessica A. Bevington, Rebecca J. Bengtsson, Nicolas Wenner, Ella V. Rodwell, Benjamin Kumwenda, Xiaojun Zhu, Rebecca J. Bennett, George E. Stenhouse, P. Malaka De Silva, Hermione J. Webster, Jose A. Bengoechea, Amy Dumigan, Alicia Tran-Dien, Reenesh Prakash, Happy C. Banda, Lovemore Alufandika, Mike P. Mautanga, Arthur Bowers-Barnard, Alexandra Y. Beliavskaia, Alexander V. Predeus, Will P. M. Rowe, Alistair C. Darby, Neil Hall, Francois-Xavier Weill, Melita A. Gordon, Nicholas A. Feasey, Kate S. Baker, Jay C. D. Hinton
Summary: This study identified a new lineage of nontyphoidal Salmonella, ST313 L3, which emerged in Malawi in 2016 and is closely related to variants causing gastrointestinal disease in the United Kingdom and Brazil. Genomic analysis revealed degradation events in important virulence genes in ST313 L3, a phenomenon not seen in other ST313 lineages.
NATURE MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria A. Campanero-Rhodes, Ioanna Kalograiaki, Begona Euba, Enrique Llobet, Ana Arda, Jesus Jimenez-Barbero, Junkal Garmendia, Dolores Solis
Summary: This study demonstrated that four human galectins can bind to Gram-negative bacteria Klebsiella pneumoniae and non-typeable Haemophilus influenzae, showing versatility in recognizing different bacterial structures. Galectins-3, -4, and -8 were found to bind to both types of bacteria, with galectin-1 lacking this ability. The results highlight the potential existence of previously overlooked galectin ligands on bacterial surfaces.
Correction
Microbiology
Axel B. Janssen, Dennis J. Doorduijn, Grant Mills, Malbert R. C. Rogers, Marc J. M. Bonten, Suzan H. M. Rooijakkers, Rob J. L. Willems, Jose A. Bengoechea, Willem Van Schaik
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Juan Nogales, Junkal Garmendia
Summary: This article provides an overview of the recent understanding of bacterial metabolism and the interaction between bacterial and host metabolism during infection. It also highlights the key outcomes and challenges for practical implementation of bacterial metabolic modelling computational tools in the pathogenesis field.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Rajendra Kumar Gurumurthy, Stefanie Koster, Naveen Kumar, Thomas F. Meyer, Cindrilla Chumduri
Summary: The cervix is a crucial part of the female reproductive system, playing important roles in fertility, pregnancy maintenance, and infection prevention. Current in vitro models for studying cervix biology are limited in reflecting the physiological and functional properties of the two types of cervical epithelium. This study presents a protocol for establishing adult epithelial stem cell-derived endocervical and ectocervical organoids, which recapitulate the native tissue architecture and can be used to study cervix biology, infections, and cancer evolution.
Article
Microbiology
Nahikari Lopez-Lopez, David San Leon, Sonia de Castro, Roberto Diez-Martinez, Manuel Iglesias-Bexiga, Maria Jose Camarasa, Margarita Menendez, Juan Nogales, Junkal Garmendia
Summary: This study focuses on the human-adapted pathogen Haemophilus influenzae and identifies new metabolic drug targets through the construction of a high-quality genome-scale metabolic model. The results provide valuable insights into potential drug targets and validate the effectiveness of a specific inhibitor against the targeted enzyme.
Article
Cell Biology
Claudia Feriotti, Joana Sa-Pessoa, Ricardo Calderon-Gonzalez, Lili Gu, Brenda Morris, Ryoichi Sugisawa, Jose L. Insua, Michael Carty, Amy Dumigan, Rebecca J. Ingram, Adrien Kissenpfening, Andrew G. Bowie, Jose A. Bengoechea
Summary: Klebsiella pneumoniae uses SARM1 protein to counteract host immunity and regulate inflammation and MAP kinase activation. Inhibiting SARM1 may be beneficial for treating Klebsiella infections.
Article
Immunology
Juan Manuel Coya, Victor Fraile-agreda, Lidia de Tapia, Belen Garcia-Fojeda, Alejandra Saenz, Jose A. Bengoechea, Nina Kronqvist, Jan Johansson, Cristina Casals
Summary: The study shows that human SP-A and a recombinant trimeric fragment (rfhSP-A) have cooperative antimicrobial activity with antibiotics against pathogenic Gram-negative bacteria. The results improve our understanding of the antimicrobial actions of SP-A and its synergistic action with polymyxin B, providing potential therapeutic options for the global problem of antibiotic resistance.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Amy Dumigan, Oisin Cappa, Brenda Morris, Joana Sa Pessoa, Ricardo Calderon-Gonzalez, Grant Mills, Rebecca Lancaster, David Simpson, Adrien Kissenpfennig, Jose A. Bengoechea
Summary: This study investigates a novel mechanism by which the human pathogen Klebsiella pneumoniae counteracts host defense by skewing lung macrophage polarization. The authors found that Klebsiella hijacks a TLR-type I IFN-IL10-STAT6 axis to rewire macrophages and promote its intracellular survival. Additionally, glycolysis and capsule polysaccharide were identified as key factors governing the macrophage polarization induced by Klebsiella. This research provides important insights into the understanding of bacterial infections and the development of therapeutic strategies.
EMBO MOLECULAR MEDICINE
(2022)
Article
Microbiology
Ricardo Calderon-Gonzalez, Alix Lee, Guillermo Lopez-Campos, Steven J. Hancock, Joana Sa-Pessoa, Amy Dumigan, Ronan McMullan, Eric L. Campbell, Jose A. Bengoechea
Summary: Klebsiella pneumoniae is a significant cause of infections, especially antibiotic-resistant ones. It colonizes the nasopharynx and gastrointestinal tract without symptoms, but colonization of the gut is essential for infection. A new murine model has been developed to study gastrointestinal colonization without tissue dissemination, providing insights into the disease dynamics of K. pneumoniae and its colonization resistance.
Article
Biochemical Research Methods
Jack Penny, Mohammad Arefian, Gunnar N. Schroeder, Jose A. Bengoechea, Ben C. Collins
Summary: Data independent acquisition (DIA/SWATH) MS is a widely used strategy in quantitative proteomics. A recent adaptation called diaPASEF, which utilizes trapped ion mobility spectrometry (TIMS), has been developed to improve selectivity and sensitivity. This study investigated the use of an analogous gas phase fractionation (GPF) method that takes into account the ion mobility (IM) dimension for library generation in the analysis of diaPASEF data. It was found that the IM-GPF approach surpassed direct library generation and approached the performance of deep libraries, making it a pragmatic method for rapid library generation.
Article
Multidisciplinary Sciences
Joana aniani Sa-Pessoa, Sara Lopez-Montesino, Kornelia Przybyszewska, Isabel Rodriguez-Escudero, Helina Marshall, Adelia Ova, Gunnar N. Schroeder, Peter Barabas, Maria Molina, Tim Curtis, Victor J. Cid, Jose A. Bengoechea
Summary: The trans-kingdom antimicrobial T6SS effector VgrG4 from Klebsiella pneumoniae can manipulate eukaryotic cells by altering the mitochondrial network. VgrG4 colocalizes with the endoplasmic reticulum protein mitofusin 2. VgrG4 induces the transfer of calcium from the endoplasmic reticulum to the mitochondria, activating the regulator of mitochondrial fission, Drp1, resulting in mitochondrial network fragmentation.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Begona Euba, Celia Gil-Campillo, Javier Asensio-Lopez, Nahikari Lopez-Lopez, Emel Sen-Kilic, Roberto Diez-Martinez, Saioa Burgui, Mariette Barbier, Junkal Garmendia
Summary: Using transcriptome sequencing, we analyzed the gene expression of both the host and pathogen during H. influenzae infection in the murine airways. We found that the host upregulates inflammatory response genes while downregulating cell adhesion and cytoskeleton genes upon infection. Additionally, H. influenzae has a requirement for purine nucleotide synthesis during infection, suggesting that targeting this metabolic process could be a potential therapeutic strategy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Irene Rodriguez-Arce, Xabier Morales, Mikel Ariz, Begona Euba, Nahikari Lopez-Lopez, Maider Esparza, Derek W. Hood, Jose Leiva, Carlos Ortiz-de-Solorzano, Junkal Garmendia
Summary: A mouse model of recurrent bacterial infection of the emphysematous lung was established to study the COPD frequent exacerbator phenotype. The results showed that recovery from frequent exacerbations took longer, and azithromycin successfully cleared recurrent infections.
Article
Microbiology
Axel B. Janssen, Dennis J. Doorduijn, Grant Mills, Malbert R. C. Rogers, Marc J. M. Bonten, Suzan H. M. Rooijakkers, Rob J. L. Willems, Jose A. Bengoechea, Willem van Schaik
Summary: Colistin resistance in Klebsiella pneumoniae can rapidly develop through diverse evolutionary trajectories, shortening the lifespan of this last-resort antibiotic for treating infections with multidrug-resistant strains.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)