Article
Microbiology
Hongchao Zhou, Yuting Zhang, Jingjing Wang, Yuchao Yan, Yi Liu, Xiaojie Shi, Qi Zhang, Xingang Xu
Summary: PEDV infection enhances CCN1 expression by activating CREB and AP-1. Overexpression of CCN1 inhibits PEDV replication and promotes apoptosis, while knockdown of CCN1 promotes PEDV replication and inhibits apoptosis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kangli Peng, Shirong Wang, Ruochuan Liu, Li Zhou, Geon H. Jeong, In Ho Jeong, Xianpeng Liu, Hiroaki Kiyokawa, Bingzhong Xue, Bo Zhao, Hang Shi, Jun Yin
Summary: This study identified a novel mechanism for regulating glycolysis and lipid metabolism through UBE3A-mediated ubiquitination of PDHA1 and ACAT1. A high-fat diet suppressed UBE3A expression in the liver, leading to increased ACAT1 protein levels and promoting the development of NAFLD.
Article
Multidisciplinary Sciences
Jalin A. Stelzer, Jason J. Yi
Summary: This article describes a rapid and convenient cell-based method for studying Ube3a gene variants. The method can be used to determine the magnitude of activity changes in any Ube3a variant, providing further insights into the enzymatic mechanism of UBE3A.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Oncology
Xinxin Rao, Peiyuan Tang, Yuanchuang Li, Guoxiang Fu, Shengzhi Chen, Xiaoya Xu, Yi Zhou, Xiaomeng Li, Long Zhang, Shaobo Mo, Sanjun Cai, Junjie Peng, Zhen Zhang, Jianjun Gao, Guoqiang Hua
Summary: CBP/P300 inhibitors have been found to effectively mitigate radiation-induced gastrointestinal syndrome (RIGS) by promoting organ and crypt regeneration, and delaying intestinal epithelial cell cycle progression, leading to improved mouse survival.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Cell Biology
Yanlu Xiong, Yangbo Feng, Jinbo Zhao, Jie Lei, Tianyun Qiao, Yongsheng Zhou, Qiang Lu, Tao Jiang, Lintao Jia, Yong Han
Summary: Our study confirmed that TFAP2A is highly expressed in LUAD, leading to poorer prognosis in multiple datasets. While TFAP2A is not essential for LUAD proliferation and can even inhibit it, it does promote metastasis through EMT. Additionally, TFAP2A activates PSG9 to enhance TGF-beta-triggered EMT, and the upregulation of TFAP2A in LUAD is partly due to the suppression of miR-16 family.
CELL DEATH & DISEASE
(2021)
Review
Genetics & Heredity
F. Isabella Zampeta, Ben Distel, Ype Elgersma, Rik Iping
Summary: This study reviews the development of Angelman syndrome research over the past 50 years using a bibliometric approach. The results reveal a shift in research focus towards genetic therapies for Angelman syndrome and provide insights into changes in funding sources, publishing journals, and international collaborations.
Article
Cell Biology
Ines C. Osma-Garcia, Dunja Capitan-Sobrino, Mailys Mouysset, Yann Aubert, Orlane Maloudi, Martin Turner, Manuel D. Diaz-Munoz
Summary: This study reveals the importance of tight regulation of RNA splicing by TIA1 and TIAL1 for the expression of integrative transcriptional programs that control DNA damage sensing and repair during B cell development.
Article
Medicine, General & Internal
David E. Godler, Ling Ling, Dinusha Gamage, Emma K. Baker, Minh Bui, Michael J. Field, Carolyn Rogers, Merlin G. Butler, Alessandra Murgia, Emanuela Leonardi, Roberta Polli, Charles E. Schwartz, Cindy D. Skinner, Angelica M. Alliende, Lorena Santa Maria, James Pitt, Ronda Greaves, David Francis, Ralph Oertel, Min Wang, Cas Simons, David J. Amor
Summary: The findings of this study suggest that screening for all chromosome 15 imprinting disorders using SNRPN methylation analysis is feasible, with 5 individuals identified out of 16,579 infants screened.
Article
Surgery
Longxiang Tu, Zunwen Lin, Qin Huang, Dewu Liu
Summary: Hypertrophic scars are fibroproliferative disorders caused by skin injury. USP15 enhances the functions of hypertrophic scar-derived fibroblasts, including proliferation, migration, invasion, and collagen deposition, by deubiquitinating T beta RI.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Article
Virology
Marcel Chris Conrady, Irina Suarez, Gergo Gogl, Desiree Isabella Frecot, Anna Bonhoure, Camille Kostmann, Alexandra Cousido-Siah, Andre Mitschler, JiaWen Lim, Murielle Masson, Thomas Iftner, Frank Stubenrauch, Gilles Trave, Claudia Simon
Summary: The study compared the structures and efficiency of ternary complex formation between oncogenic E6 proteins of closely related high-risk HPV31 and HPV16 with their cellular targets p53 and E6AP. Despite high structural similarity, 31 E6 showed an overall lower binding affinity than 16 E6, aligning with the observed lower p53 degradation potential of 31 E6.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Rossella Avagliano Trezza, A. Mattijs Punt, Edwin Mientjes, Marlene van den Berg, F. Isabella Zampeta, Ilona J. de Graaf, Yana van der Weegen, Jeroen A. A. Demmers, Ype Elgersma, Ben Distel
Summary: Research found that the synaptic protein RPH3A may become a target of ubiquitination by UBE3A, a gene commonly mutated in the neurodevelopmental disorder AS. However, this ubiquitination process does not lead to degradation of RPH3A, suggesting a non-degradative function of UBE3A-dependent ubiquitination in this context.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Jeffrey R. White, Dakota T. Thompson, Kelsey E. Koch, Boris S. Kiriazov, Anna C. Beck, Dana M. van der Heide, Benjamin G. Grimm, Mikhail V. Kulak, Ronald J. Weigel
Summary: In melanoma metastasis, the role of the transcription factor AP-2 alpha is controversial, with some studies suggesting tumor suppressor activity while others show it facilitates metastasis by upregulating E2F pathway genes including EZH2. AP-2 alpha interacts with the NuRD complex, and its loss results in removal of activating chromatin marks and promotion of metastasis. These findings suggest AP-2 alpha can serve as a biomarker for predicting response to EZH2 inhibitors in treating advanced melanomas.
Article
Cell Biology
Ribhav Mishra, Vibhuti Joshi, Arun Upadhyay, Ayeman Amanullah, Ankur Rakesh Dubey, Sarika Singh, Vikash Kumar Dubey, Krishna Mohan Poluri, Nihar Ranjan Jana, Amit Mishra
Summary: Cells maintain optimal cellular physiology through various protein quality control mechanisms, including molecular chaperones, the ubiquitin-proteasome system, and autophagy. A study showed that the E3 ubiquitin ligase LRSAM1 promotes the degradation of the quality control E3 ubiquitin ligase E6-AP, affecting cell cycle regulatory proteins and proliferation rate. This finding offers insights into potential therapeutic approaches for protein aggregation diseases.
CELLULAR SIGNALLING
(2021)
Article
Environmental Sciences
Hongli Wang, Min Zhang, Xiuduan Xu, Shaojun Hou, Zhihui Liu, Xuejiao Chen, Chongchong Zhang, Huan Xu, Lin Wu, Kun Liu, Lun Song
Summary: This study revealed that IKK alpha played a critical role in mediating UVB-induced apoptotic responses in UVB sensitive cells by regulating the activation of the p53 pathway. Targeting the IKK alpha/p53/PERP pathway could be a potential strategy to prevent skin photo-damages induced by sunlight.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Fisheries
Shanshan Zeng, Meifeng Li, Xining Cheng, Shina Lu, Zhiqing Feng, Zeyin Jiang, Zhichao Sun, Xiaowen Xu, Huiling Mao, Chengyu Hu
Summary: In this study, it was found that grass carp DYRK2 induces cell apoptosis by increasing the phosphorylation level of p53.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Clinical Neurology
Arun Upadhyay, Naveen Sundaria, Rohan Dhiman, Vijay Kumar Prajapati, Amit Prasad, Amit Mishra
Summary: A healthy physiological environment in cells is maintained through the dynamic homeostasis of crowded molecules, with a subset of cellular proteome forming the protein quality control machinery. However, specific conditions such as genetic mutations, environmental stress, and aging can overwhelm the protein quality control system, leading to the generation of abnormal protein species and the formation of inclusion bodies which may be toxic or protective. The detailed interplay of these inclusion bodies in the manifestation of neurodegenerative disorders is still unknown, with limited knowledge of their structural compositions.
Review
Biochemistry & Molecular Biology
Ankur R. Dubey, Yuvraj A. Jagtap, Prashant Kumar, Som M. Patwa, Sumit Kinger, Amit Kumar, Sarika Singh, Amit Prasad, Nihar R. Jana, Amit Mishra
Summary: The article discusses the interaction between viruses and E3 Ubiquitin Ligases, highlighting the molecular pathomechanism of viruses linked with E3 Ubiquitin Ligases dependent mechanisms. An enhanced understanding of E3 Ubiquitin Ligase-mediated removal of viral proteins may open new therapeutic strategies against viral infections.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Review
Cell Biology
Prashant Kumar, Yuvraj Anandrao Jagtap, Som Mohanlal Patwa, Sumit Kinger, Ankur Rakesh Dubey, Vijay Kumar Prajapati, Rohan Dhiman, Krishna Mohan Poluri, Amit Mishra
Summary: Evidence suggests that defective proteostasis may contribute to cancer risk factors, while the autophagy mechanism plays a key role in maintaining normal cellular homeostasis to prevent tumorigenesis. Despite the importance of autophagy in tumor suppression, the precise molecular mechanisms are not yet clear.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Abhishek Singh, Parul Gupta, Shubhangini Tiwari, Amit Mishra, Sarika Singh
Summary: The study revealed that guanabenz has a comprehensive protective effect against multiple degenerative markers and signaling pathways associated with Alzheimer's disease, indicating potential as a new therapeutic agent.
CELL AND TISSUE RESEARCH
(2022)
Article
Immunology
Rupal Ojha, Kiran Gurjar, Tadi Sai Ratnakar, Amit Mishra, Vijay Kumar Prajapati
Summary: COVID-19 originated in Wuhan, China and has caused a global pandemic. This study focuses on designing a bispecific antibody to block viral entry and potentially provide a new treatment option.
Article
Biochemistry & Molecular Biology
Omkar Indari, Shweta Jakhmola, Devesh K. Pathak, Manushree Tanwar, Meenakshi Kandpal, Amit Mishra, Rajesh Kumar, Hem Chandra Jha
Summary: Raman microspectroscopy was used to investigate the biomolecular changes in EBV-infected neuronal cells. The study found that EBV infection can regulate the biomolecular environment in neuronal cells, astrocytes, and microglia to different extents, and identified common and unique biomolecules in each cell type. These findings contribute to the understanding of EBV's role in the central nervous system.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Biology
Neha Jain, Md Fulbabu Sk, Amit Mishra, Parimal Kar, Amit Kumar
Summary: This study used a pharmacophore-based approach and computational methods to search for novel inhibitors against Neisseria gonorrhoeae's antibiotic resistance. Through an integrated computational framework, five compounds with higher binding affinity and safety were found, which can be used for the generation of novel EPIs.
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Satyendra Singh, Ketan Kumar, Mamta Panda, Aryan Srivastava, Amit Mishra, Vijay Kumar Prajapati
Summary: Immunotherapy using immune checkpoint inhibitors has been widely used for treating various cancers. This study screened small-molecule inhibitors against immune cell checkpoints and identified three potential inhibitors that showed the ability to activate human immune cells and control the spread of diseases. Further validation is needed for development as immunotherapeutics.
MOLECULAR DIVERSITY
(2023)
Article
Neurosciences
Ankur Rakesh Dubey, Som Mohanlal Patwa, Sumit Kinger, Yuvraj Anandrao Jagtap, Prashant Kumar, Sarika Singh, Rohan Dhiman, Hem Chandra Jha, Amit Mishra
Summary: Cells synthesize new proteins after multiple molecular decisions, and protein quality control (PQC) can cope against proteostasis imbalance through alternative strategies. Accumulation of misfolded proteins is linked with neurodegenerative disorders, and understanding the abnormalities of PQC coupled molecules can help in finding biomarkers associated with neuronal disorders.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Neurosciences
K. Amrutha, Amit Mishra, Sarika Singh
Summary: Parkinson's disease is the most common motor neurodegenerative disease characterized by the degeneration of dopamine neurons. The presence of alpha-synuclein-containing Lewy bodies suggests the involvement of diminished protein degradation mechanisms in disease development. However, the exact relationship between these aggregates and disease pathology requires further investigation.
JOURNAL OF NEUROSCIENCE RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Anjali Sangeeth, Mahesh Malleswarapu, Amit Mishra, Ravi Kumar Gutti
Summary: Long non-coding RNAs (lncRNAs) are more than 200 bases long and have major regulatory roles in biologic processes such as hematopoeisis and metabolism. Recent studies have shown that lncRNAs directly and indirectly participate in metabolism by regulating enzymes and signaling pathways, and can alter tissue-specific metabolism. Understanding the different functions of lncRNAs in cellular pathways helps in predicting and treating myelodysplastic and metabolic disorders. This review provides an overview of ncRNAs, their effects on hematopoiesis and metabolism, and discusses their prognostic value and potential as biomarkers and therapeutic targets.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Shubhangini Tiwari, Parul Gupta, Abhishek Singh, Swati Chaturvedi, M. Wahajuddin, Amit Mishra, Sarika Singh
Summary: This study investigated the therapeutic effects of the drug 4-phenylbutyric acid (4PBA) in Parkinson's disease (PD) and found that it can protect neurons, reduce oxidative stress and calcium levels, inhibit neuronal apoptosis, restore neuronal morphology, and inhibit astrocyte activation.
NEUROCHEMICAL RESEARCH
(2022)
Review
Oncology
Ayeman Amanullah, Arun Upadhyay, Rohan Dhiman, Sarika Singh, Amit Kumar, Dinesh Kumar Ahirwar, Ravi Kumar Gutti, Amit Mishra
Summary: This article summarizes the current understanding of the drug diclofenac, discussing its potential applications and existing limitations. It highlights the need for further research to better understand its mechanisms and improve its effectiveness against various diseases.
Review
Cell Biology
Sumit Kinger, Ankur Rakesh Dubey, Prashant Kumar, Yuvraj Anandrao Jagtap, Akash Choudhary, Amit Kumar, Vijay Kumar Prajapati, Rohan Dhiman, Amit Mishra
Summary: Amyotrophic lateral sclerosis (ALS) is a neuronal degenerative condition caused by the accumulation of mutant aberrantly folded proteins. The involvement of chaperones in ALS pathogenesis is not thoroughly understood, making it challenging to develop effective treatments for the disease.
Review
Biochemistry & Molecular Biology
Annu Rani, Vaishali Saini, Priyanka Patra, Tanish Prashar, Rajan Kumar Pandey, Amit Mishra, Hem Chandra Jha
Summary: EGCG, a polyphenolic compound in green tea, has pleiotropic bioactivities to combat neurological diseases and inhibit the effects of neurotropic viruses.
ACS CHEMICAL NEUROSCIENCE
(2023)