Article
Chemistry, Medicinal
Xinnan Li, Tiantian Li, Pengfei Zhang, Xinuo Li, Li Lu, Yuan Sun, Bocheng Zhang, Stephanie Allen, Lisa White, James Phillips, Zheying Zhu, Hequan Yao, Jinyi Xu
Summary: Novel compounds have been discovered for the potential treatment of Alzheimer's disease (AD) using a multitarget strategy. These compounds showed significant inhibitory activity against acetylcholinesterase (AChE) and demonstrated various beneficial pharmacological activities in vitro and in vivo, including antioxidant, neuroprotective, and anti-Tau phosphorylation effects.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yu-Shen Luo, Quan-Kuo He, Ming-Xin Sun, Feng-Xin Qiao, Yue-Cen Liu, Chang-Long Xu, Zhi-Ran Xu, Si-Cheng Zhao, Hai-Long Wang, Zhong-Quan Qi, Yu Liu
Summary: Acrylonitrile treatment disrupts ovarian homeostasis, leading to impaired follicular development and reduced offspring. This is associated with inflammation, apoptosis, DNA damage, oxidative stress, endoplasmic reticulum abnormalities, and lysosomal aggregation caused by acrylonitrile exposure.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Chemistry, Multidisciplinary
Estefania Zuriaga, Laura Lomba, Cristina B. Garcia, Marta Sofia Valero
Summary: Nowadays, the scientific community is seeking and developing green solvents, derived from biomass, that can replace traditional solvents. A study was conducted to analyze the cytotoxicity of eleven biomass-derived solvents in terms of biochemical pathways and cell morphology. Results showed that most solvents in the furfural family exhibited higher toxicity, while other solvents also caused cellular damage and stress responses.
Article
Biochemistry & Molecular Biology
Jingjing Pan, Yanfeng Wei, Linlin Ni, Xiaoyang Li, Yu Deng, Bin Xu, Tianyao Yang, Jingyi Sun, Wei Liu
Summary: This study investigates the role of calcium ion (Ca2+) homeostasis in MeHg-induced neurotoxicity. The results suggest that MeHg causes cell damage and increases Ca2+ release from the ER as well as free Ca2+ levels in the cytoplasm and mitochondria. Ca2+ channel inhibitors and Ca2+ chelator can partially inhibit MeHg-induced mitochondrial damage and apoptosis.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Review
Cell Biology
Xun Gao, Yuanyuan Xu
Summary: Alzheimer's disease is the most common neurodegenerative disease characterized by progressive cognitive impairment and memory loss. Research indicates that endoplasmic reticulum stress and unfolded protein response play a role in the pathogenesis of AD. Targeting ER stress and ER stress-mediated apoptosis may be effective in treating AD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Vivian W. R. Moraes, Vivian M. Santos, Eloah R. Suarez, Leticia S. Ferraz, Rayssa de Mello Lopes, Giuliana P. Mognol, Joana D. Campeiro, Joao A. Machado-Neto, Fabio D. Nascimento, Mirian A. F. Hayashi, Ivarne L. S. Tersariol, Donald D. Newmeyer, Tiago Rodrigues
Summary: Mitochondria play crucial roles in cellular physiology, and their dysregulation has been linked to cancer cells. Thioridazine (TR) induces cytotoxicity in human leukemia cells through a mechanism involving calcium imbalance, mitochondrial permeabilization, endoplasmic reticulum stress, and apoptosis. Additionally, TR shows potential as an adjuvant in antitumor chemotherapy.
Article
Multidisciplinary Sciences
Yves Gouriou, Fabrice Gonnot, Mariam Wehbi, Camille Brun, Ludovic Gomez, Gabriel Bidaux
Summary: Genetically encoded biosensors based on fluorescent proteins (FPs) are widely used to monitor calcium ion (Ca2+) dynamics and sub-cellular spatial distribution in cells. The mitochondrial-associated membranes (MAMs), which connect the endoplasmic reticulum (ER) and mitochondria, have recently gained attention due to their role in calcium homeostasis and mitochondrial function. However, the sensitivity of the existing Ca2+ biosensors on the outer-mitochondrial membrane (OMM) is not sufficient to measure sub-micromolar changes in Ca2+ concentration. In this study, a new highly sensitive Ca2+ biosensor was developed and demonstrated to detect smaller differences in Ca2+ levels in or near the MAMs. Notably, the study also revealed the endogenous activity of IP3-receptors in the Ca2+ leak channel on the OMM during hypoxia or when SERCA activity is blocked.
Article
Pharmacology & Pharmacy
Yuanyong Jiao, Xiwei Zhang, Hongyu Yang, Hao Ma, Junjie Zou
Summary: This study investigates the absorption and toxic action of polyethylene glycol-modified mesoporous tantalum oxide nanomaterials using human cardio microvascular endothelial cells. The study finds that these nanomaterials can promote endothelial cell apoptosis and the mechanism may involve the activation of the endoplasmic reticulum stress-mitochondrial cascade.
Article
Biochemistry & Molecular Biology
Benjamin Guieu, Cedric Lecoutey, Remi Legay, Audrey Davis, Jana Sopkova de Oliveira Santos, Cosimo Damiano Altomare, Marco Catto, Christophe Rochais, Patrick Dallemagne
Summary: Alzheimer's disease is a multifactorial neurodegenerative disease, and using Multi-Target Directed Ligands is considered convenient in targeting validated factors like acetylcholinesterase and Monoamine Oxidase-B. Ladostigil, currently in clinical trials, shows promising results in inhibiting these factors. The synthesis of a compound, propargylaminodonepezil, showed inhibitory activities towards AChE and MAO-B.
Article
Chemistry, Medicinal
Gabriela Dumitrita Stanciu, Daniela Carmen Ababei, Carmen Solcan, Veronica Bild, Andrei Ciobica, Sorin-Ioan Beschea Chiriac, Loredana Maria Ciobanu, Bogdan-Ionel Tamba
Summary: The incidence of neurodegenerative diseases, such as Alzheimer's disease (AD), is continuously growing worldwide, leading to a significant burden on society and economy. Recent studies have shown that sodium-glucose co-transporter 2 inhibitors (SGLT2i) may have neuroprotective effects and improve AD-related behaviors and brain pathology. These findings highlight the potential importance of SGLT2i in clinical practice.
Review
Biochemistry & Molecular Biology
Robert E. Means, Samuel G. Katz
Summary: The outer mitochondrial membrane is essential for cellular survival, with its mitochondria-associated membranes serving as key signaling hubs. Different subsets of MAMs play important roles in regulating cellular homeostasis, with various BCL-2 family members contributing to their functions.
Article
Environmental Sciences
Siyu Li, Pengfei Wu, Bing Han, Qingyue Yang, Xiaoqiao Wang, Jiayi Li, Ning Deng, Biqi Han, Yuge Liao, Yan Liu, Zhigang Zhang
Summary: This study investigated the effect of DLM on quail cerebrum neurons and found that DLM exposure induced histological changes, oxidative stress, and neurons apoptosis in quail cerebrum tissues. Furthermore, the study showed that DLM exposure also increased the expression of ER stress-related mRNA and decreased ATP content in quail cerebrum tissues, indicating ER stress and mitochondrial dysfunction as possible mechanisms for DLM-induced apoptosis of quail cerebrum neurons.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Oncology
Chen-Lin Yu, Hsiang-Lin Lee, Shun-Fa Yang, Shih-Wei Wang, Ching-Pin Lin, Yi-Hsien Hsieh, Hui-Ling Chio
Summary: In this study, we found that protodioscin (PD) can induce apoptosis, loss of mitochondrial membrane potential, and endoplasmic reticulum (ER) expansion in hepatocellular carcinoma (HCC) cells. Mechanistic studies revealed that PD facilitates the transfer of Ca2+ from ER to mitochondria and induces apoptosis by forming a complex with Mfnl and Bak. In addition, our animal model further validated the anti-tumor effect of PD.
JOURNAL OF HEPATOCELLULAR CARCINOMA
(2022)
Article
Gastroenterology & Hepatology
Liuyi Hao, Wei Zhong, Haibo Dong, Wei Guo, Xinguo Sun, Wenliang Zhang, Ruichao Yue, Tianjiao Li, Alexandra Griffiths, Ali Reza Ahmadi, Zhaoli Sun, Zhenyuan Song, Zhanxiang Zhou
Summary: The study reveals that hepatic ATF4 plays a pathological role in alcohol-induced mitochondrial dysfunction and liver injury by disrupting the NRF1-TFAM pathway. In patients with alcoholic liver disease, the activation of ATF4 is associated with mitochondrial dysfunction. Knocking out ATF4 or overexpressing TFAM can alleviate alcohol-induced liver damage. This is achieved through the negative regulation of TFAM expression by ATF4, affecting ethanol-mediated mitochondrial dysfunction and cell death.
Article
Plant Sciences
Yingli Yu, Min Wang, Rongchang Chen, Xiao Sun, Guibo Sun, Xiaobo Sun
Summary: GP-17 provides cardioprotective effects by inhibiting myocardial apoptosis, reducing cardiac dysfunction, and improving contractile recovery. The study results demonstrate that GP-17 inhibits I/R-induced mitochondrial injury by delaying the onset of ER stress, which is controlled by the PI3K/AKT and P38 signaling pathways.
JOURNAL OF GINSENG RESEARCH
(2021)
Review
Clinical Neurology
Arun Upadhyay, Naveen Sundaria, Rohan Dhiman, Vijay Kumar Prajapati, Amit Prasad, Amit Mishra
Summary: A healthy physiological environment in cells is maintained through the dynamic homeostasis of crowded molecules, with a subset of cellular proteome forming the protein quality control machinery. However, specific conditions such as genetic mutations, environmental stress, and aging can overwhelm the protein quality control system, leading to the generation of abnormal protein species and the formation of inclusion bodies which may be toxic or protective. The detailed interplay of these inclusion bodies in the manifestation of neurodegenerative disorders is still unknown, with limited knowledge of their structural compositions.
Review
Biochemistry & Molecular Biology
Ankur R. Dubey, Yuvraj A. Jagtap, Prashant Kumar, Som M. Patwa, Sumit Kinger, Amit Kumar, Sarika Singh, Amit Prasad, Nihar R. Jana, Amit Mishra
Summary: The article discusses the interaction between viruses and E3 Ubiquitin Ligases, highlighting the molecular pathomechanism of viruses linked with E3 Ubiquitin Ligases dependent mechanisms. An enhanced understanding of E3 Ubiquitin Ligase-mediated removal of viral proteins may open new therapeutic strategies against viral infections.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Review
Cell Biology
Prashant Kumar, Yuvraj Anandrao Jagtap, Som Mohanlal Patwa, Sumit Kinger, Ankur Rakesh Dubey, Vijay Kumar Prajapati, Rohan Dhiman, Krishna Mohan Poluri, Amit Mishra
Summary: Evidence suggests that defective proteostasis may contribute to cancer risk factors, while the autophagy mechanism plays a key role in maintaining normal cellular homeostasis to prevent tumorigenesis. Despite the importance of autophagy in tumor suppression, the precise molecular mechanisms are not yet clear.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Immunology
Rupal Ojha, Kiran Gurjar, Tadi Sai Ratnakar, Amit Mishra, Vijay Kumar Prajapati
Summary: COVID-19 originated in Wuhan, China and has caused a global pandemic. This study focuses on designing a bispecific antibody to block viral entry and potentially provide a new treatment option.
Article
Biochemistry & Molecular Biology
Omkar Indari, Shweta Jakhmola, Devesh K. Pathak, Manushree Tanwar, Meenakshi Kandpal, Amit Mishra, Rajesh Kumar, Hem Chandra Jha
Summary: Raman microspectroscopy was used to investigate the biomolecular changes in EBV-infected neuronal cells. The study found that EBV infection can regulate the biomolecular environment in neuronal cells, astrocytes, and microglia to different extents, and identified common and unique biomolecules in each cell type. These findings contribute to the understanding of EBV's role in the central nervous system.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Biology
Neha Jain, Md Fulbabu Sk, Amit Mishra, Parimal Kar, Amit Kumar
Summary: This study used a pharmacophore-based approach and computational methods to search for novel inhibitors against Neisseria gonorrhoeae's antibiotic resistance. Through an integrated computational framework, five compounds with higher binding affinity and safety were found, which can be used for the generation of novel EPIs.
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Satyendra Singh, Ketan Kumar, Mamta Panda, Aryan Srivastava, Amit Mishra, Vijay Kumar Prajapati
Summary: Immunotherapy using immune checkpoint inhibitors has been widely used for treating various cancers. This study screened small-molecule inhibitors against immune cell checkpoints and identified three potential inhibitors that showed the ability to activate human immune cells and control the spread of diseases. Further validation is needed for development as immunotherapeutics.
MOLECULAR DIVERSITY
(2023)
Article
Neurosciences
Ankur Rakesh Dubey, Som Mohanlal Patwa, Sumit Kinger, Yuvraj Anandrao Jagtap, Prashant Kumar, Sarika Singh, Rohan Dhiman, Hem Chandra Jha, Amit Mishra
Summary: Cells synthesize new proteins after multiple molecular decisions, and protein quality control (PQC) can cope against proteostasis imbalance through alternative strategies. Accumulation of misfolded proteins is linked with neurodegenerative disorders, and understanding the abnormalities of PQC coupled molecules can help in finding biomarkers associated with neuronal disorders.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Anjali Sangeeth, Mahesh Malleswarapu, Amit Mishra, Ravi Kumar Gutti
Summary: Long non-coding RNAs (lncRNAs) are more than 200 bases long and have major regulatory roles in biologic processes such as hematopoeisis and metabolism. Recent studies have shown that lncRNAs directly and indirectly participate in metabolism by regulating enzymes and signaling pathways, and can alter tissue-specific metabolism. Understanding the different functions of lncRNAs in cellular pathways helps in predicting and treating myelodysplastic and metabolic disorders. This review provides an overview of ncRNAs, their effects on hematopoiesis and metabolism, and discusses their prognostic value and potential as biomarkers and therapeutic targets.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Shubhangini Tiwari, Parul Gupta, Abhishek Singh, Swati Chaturvedi, M. Wahajuddin, Amit Mishra, Sarika Singh
Summary: This study investigated the therapeutic effects of the drug 4-phenylbutyric acid (4PBA) in Parkinson's disease (PD) and found that it can protect neurons, reduce oxidative stress and calcium levels, inhibit neuronal apoptosis, restore neuronal morphology, and inhibit astrocyte activation.
NEUROCHEMICAL RESEARCH
(2022)
Article
Cell Biology
Shubhangini Tiwari, Abhishek Singh, Parul Gupta, Sarika Singh
Summary: This study reveals the crucial role of UBA52 in regulating protein aggregation and cell death in Parkinson's disease pathology. UBA52 interacts with alpha-synuclein, HSP90, and E3-ubiquitin ligase CHIP, and co-localizes with alpha-synuclein in the mitochondrion. Furthermore, UBA52 plays an essential role in HSP90 ubiquitylation.
Article
Biochemistry & Molecular Biology
Shubhangini Tiwari, Abhishek Singh, Parul Gupta, K. Amrutha, Sarika Singh
Summary: This study investigates the role of the ubiquitin-encoding gene UBA52 in mitochondrial physiology. UBA52 interacts with VDAC1, and its overexpression protects neurons from oxidative stress and improves mitochondrial functionality and cell viability. This provides new insights into the prevention of dopaminergic cell death during acute Parkinson's disease pathogenesis.
ACS CHEMICAL NEUROSCIENCE
(2023)
Review
Cell Biology
Sumit Kinger, Ankur Rakesh Dubey, Prashant Kumar, Yuvraj Anandrao Jagtap, Akash Choudhary, Amit Kumar, Vijay Kumar Prajapati, Rohan Dhiman, Amit Mishra
Summary: Amyotrophic lateral sclerosis (ALS) is a neuronal degenerative condition caused by the accumulation of mutant aberrantly folded proteins. The involvement of chaperones in ALS pathogenesis is not thoroughly understood, making it challenging to develop effective treatments for the disease.
Review
Biochemistry & Molecular Biology
Annu Rani, Vaishali Saini, Priyanka Patra, Tanish Prashar, Rajan Kumar Pandey, Amit Mishra, Hem Chandra Jha
Summary: EGCG, a polyphenolic compound in green tea, has pleiotropic bioactivities to combat neurological diseases and inhibit the effects of neurotropic viruses.
ACS CHEMICAL NEUROSCIENCE
(2023)
