Article
Chemistry, Multidisciplinary
Mizuki Tenjimbayashi, Shota Yamamoto, Koichiro Uto
Summary: In this study, a dry powder that encapsulates single to several cells with a >95% aqueous culture medium is reported as a powerful cell-picking tool. The drycells are formed by spraying a cell suspension onto a powder bed, and the particles adsorb to the droplet surface and form a superhydrophobic shell. The number of encapsulated cells per drycell can be controlled, and various handling techniques can be used.
ADVANCED MATERIALS
(2023)
Article
Plant Sciences
Eveline Y. Y. Kong, Julianne Biddle, Sundaravelpandian Kalaipandian, Steve W. W. Adkins
Summary: This study attempted to obtain friable coconut embryogenic callus (EC) through various approaches, but none of them were successful. However, some of the culture conditions were found to influence the formation of compact EC, which is important for further studies on somatic embryogenesis in coconut and other species.
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Tomoki Murakami, Hiroto Teratani, Dai'ichiro Aoki, Masao Noguchi, Mamiko Tsugane, Hiroaki Suzuki
Summary: In this study, a hydrodynamic cell-trapping system in an open microfluidics architecture was developed, allowing external access to trapped cells and enabling automated retrieval. This system bridges the gap between microfluidic devices and conventional macro-scale apparatus used in biology, fostering the development of new single-cell analytical systems.
Article
Multidisciplinary Sciences
Chia-Lung Wu, Amanda Dicks, Nancy Steward, Ruhang Tang, Dakota B. Katz, Yun-Rak Choi, Farshid Guilak
Summary: The study identified key genes such as WNT and MITF in off-target cell differentiation during chondrogenic differentiation using bulk and single cell RNA-sequencing; inhibiting these pathways can enhance cell homogeneity and yield.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Qinlei Liu, Sandra Martinez-Jarquin, Wenjie Ge, Renato Zenobi
Summary: Understanding diseases requires understanding metabolic heterogeneity in cells. This technical note introduces a 3D printing technology for manufacturing an ionization source for mass spectrometry-based single-cell analysis. The all in-one 3D-printed electrospray ionization source simplifies the process and improves reproducibility. It has been successfully used for high-throughput analysis of cancer cells and can be adjusted for different cell sizes. This 3D-printed ionization source opens new possibilities for single-cell analysis.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Shenghao Guo, Cissy Zhang, Anne Le
Summary: Single-cell metabolomics is a powerful tool for high-throughput metabolic analysis at the cellular level, with great potential in decoding cell heterogeneity and identifying cell types within colonies. The development of various equipment and techniques has made SCM analysis possible for a wide range of biological samples, leading to fruitful findings in various fields such as phytology, neurology, and oncology.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Cell Biology
Mingxi Deng, Ying Wang, Yan Yan
Summary: Recent advancements in single-cell RNA sequencing techniques have led to an increase in single-cell atlases from various biological contexts, containing crucial information on cell-cell signaling events that are essential for understanding multicellular organism function.
CURRENT OPINION IN CELL BIOLOGY
(2022)
Article
Critical Care Medicine
Minzhe Guo, Kathryn A. Wikenheiser-Brokamp, Joseph A. Kitzmiller, Cheng Jiang, Guolun Wang, Allen Wang, Sebastian Preissl, Xiaomeng Hou, Justin Buchanan, Justyna A. Karolak, Yifei Miao, David B. Frank, William J. Zacharias, Xin Sun, Yan Xu, Mingxia Gu, Pawel Stankiewicz, Vladimir V. Kalinichenko, Jennifer A. Wambach, Jeffrey A. Whitsett
Summary: This study reveals the cellular and molecular mechanisms underlying the pathogenesis of Alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV), a lethal disorder of lung development. The deficiency of FOXF1 transcription factor disrupts lung formation, leading to changes in cell types, gene expression, and cell-cell interactions in ACDMPV lungs. These findings provide potential targets for the development of therapies for ACDMPV and other diffuse lung diseases of infancy.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Review
Materials Science, Ceramics
Vanessa Modelski Schatkoski, Thais Larissa do Amaral Montanheiro, Beatriz Rossi Canuto de Menezes, Raissa Monteiro Pereira, Karla Faquine Rodrigues, Renata Guimaraes Ribas, Diego Morais da Silva, Gilmar Patrocinio Thim
Summary: The research on biomaterials has shown significant improvement in the quality of life for individuals requiring surgery. Bioceramics and bioactive glasses play a key role in hard tissue engineering, promoting tissue regeneration and multifunctionality when implanted. Incorporating specific metal ions into these biomaterials enhances their properties and expands their applications, including in cancer treatment and drug delivery.
CERAMICS INTERNATIONAL
(2021)
Editorial Material
Biochemical Research Methods
Xiaowei Zhuang
Summary: The recent development of genome-scale imaging allows for single-cell omics analysis in intact cells and tissues, enabling gene expression profiling, identification of cell types, and spatial mapping in complex tissues. The high spatial resolution of these approaches also allows for determining the spatial organizations of the genome and transcriptome inside cells, which are key regulatory mechanisms for gene expression.
Article
Multidisciplinary Sciences
Julea Vlassakis, Louise L. Hansen, Ryo Higuchi-Sanabria, Yun Zhou, C. Kimberly Tsui, Andrew Dillin, Haiyan Huang, Amy E. Herr
Summary: This research introduces a novel method to simultaneously detect protein complexes in stressed cell populations, revealing potential cellular responses to specific treatments. The study also highlights the impact of non-chemical stress on cellular heterogeneity of F-actin. The assay developed in this work overcomes selectivity limitations to biochemically quantify single-cell protein complexes perturbed with diverse stimuli.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
M. Moniruzzaman, Yun Zhong, Zhifeng Huang, Huaxue Yan, Lv Yuanda, Bo Jiang, Guangyan Zhong
Summary: In this study, a suspension cell culture system was established to generate transgenic Citrus plants, overcoming the limitations of Agrobacterium-mediated transformation of epicotyl segments. The results indicated that 8 to 10 week-old ovules were the most suitable materials for inducing embryonic calli. A high transformation rate (around 35%) was achieved using the cell suspension system, leading to the generation of non-chimeric transgenic Citrus plants.
Review
Biochemistry & Molecular Biology
Luke F. Vistain, Savas Tay
Summary: The inability to make broad, minimally biased measurements of a cell's proteome is a major bottleneck in understanding gene expression and cellular phenotype. Current methods for single-cell proteomic measurements typically focus on either absolute quantification of a small number of proteins or highly multiplexed protein measurements, with advancements in microfluidics and output encoding enabling major improvements. Emerging technologies like single-cell mass spectrometry may provide unbiased measurement of cellular proteomes.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Xin Guan, Haijuan Li, Limei Chen, Guohua Qi, Yongdong Jin
Summary: A glass capillary-based nanopore (G-nanopore) with tapered tip and flexible surface modifications provides a powerful sensing platform for diverse biological measurements. It enables noninvasive single molecule and subcellular single cell detections and shows potential for cancer diagnosis and treatment applications. However, current bottlenecks limit its widespread use in clinical applications.
Article
Clinical Neurology
Mayke Oosterloo, Emilia Bijlsma, Sander van Kuijk, Floor Minkes, Raymund Roos, C. E. M. de Die-Smulders
PARKINSONISM & RELATED DISORDERS
(2019)
Review
Neurosciences
Anders Bjorklund, Stephen B. Dunnett
JOURNAL OF PARKINSONS DISEASE
(2019)
Letter
Medicine, Research & Experimental
Gregor A. Bara, Dirk de Ridder, Jaroslaw Maciaczyk
MEDICAL HYPOTHESES
(2020)
Article
Cell Biology
Katharina Koch, Rudolf Hartmann, Julia Tsiampali, Constanze Uhlmann, Ann-Christin Nickel, Xiaoling He, Marcel A. Kamp, Michael Sabel, Roger A. Barker, Hans-Jakob Steiger, Daniel Haenggi, Dieter Willbold, Jaroslaw Maciaczyk, Ulf D. Kahlert
CELL DEATH DISCOVERY
(2020)
Article
Multidisciplinary Sciences
Dayana Herrera-Rios, Guanzhang Li, Dilaware Khan, Julia Tsiampali, Ann-Christin Nickel, Philippe Aretz, Michael Hewera, Abiagail Kora Suwala, Tao Jiang, Hans-Jakob Steiger, Marcel Alexander Kamp, Sajjad Muhammad, Daniel Hanggi, Jarek Maciaczyk, Wei Zhang, Ulf Dietrich Kahlert
SCIENTIFIC REPORTS
(2020)
Article
Engineering, Biomedical
Dirk De Ridder, Jarek Maciaczyk, Sven Vanneste
Summary: Neuromodulation is the alteration of nerve activity through targeted stimulation, with increasingly complex implantable systems expected in the near future (<5 years). In the near-to-far future (5-10 years), integration of adaptive network neuromodulation with predictive artificial intelligence is anticipated, with a long-term goal of achieving a brain with integrated artificial intelligence (>10 years).
EXPERT REVIEW OF MEDICAL DEVICES
(2021)
Article
Biochemistry & Molecular Biology
Satoshi Nakata, Ming Yuan, Jeffrey A. Rubens, Ulf D. Kahlert, Jarek Maciaczyk, Eric H. Raabe, Charles G. Eberhart
Summary: CNS-BCOR ITD is a malignant entity with a specific oncogenic mechanism, the impact on the biology of neuroepithelial cells is not well understood. BCOR-ITD promotes derepression of PRC2-target genes, but no growth advantage was observed in human neural stem cells, while enhanced cell growth, invasion, and migration were seen in murine cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Spectroscopy
Chima Robert, Julia Tsiampali, Sara J. Fraser-Miller, Silke Neumann, Donata Maciaczyk, Sarah L. Young, Jaroslaw Maciaczyk, Keith C. Gordon
Summary: Raman spectroscopy combined with chemometrics was used to distinguish the phenotype of T-cells and monocytes incubated with media conditioned by glioblastoma stem-cells, showing different molecular backgrounds. Different clusters were formed based on EMT activator ZEB1 or CD73 inhibition, and discriminatory analysis using LDA and SVM yielded sensitivities and specificities of over 70% and 67% respectively. Flow cytometric analysis revealed the influence of TCMs on cytokine profile of T-cells and monocytes, indicating the potential of Raman spectroscopy in tracking immune cell phenotypes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Oncology
Bhanupriya Dhabhai, Amit Sharma, Jarek Maciaczyk, Tikam Chand Dakal
Summary: This discussion focuses on the relative contribution of X-linked human tumor suppressor genes in various cancer types, highlighting their involvement in breast cancer and prostate cancer, as well as the observed alterations in promoter methylation patterns linked to mutational burden.
CANCER INVESTIGATION
(2022)
Article
Cell Biology
Daniel Rees, Amy L. Beynon, Mariah J. Lelos, Gaynor A. Smith, Luke D. Roberts, Lyndsey Phelps, Stephen B. Dunnett, Alwena H. Morgan, Rowan M. Brown, Timothy Wells, Jeffrey S. Davies
Summary: The feeding-related hormone, acyl-ghrelin, has a potential protective effect on dopamine neurons in experimental models of Parkinson's disease. However, its effect on dopaminergic neurons in the 6-OHDA rat medial forebrain bundle lesion model is unknown.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Neurosciences
Martin Vychopen, Johannes Wach, Valeri Borger, Matthias Schneider, Lars Eichhorn, Jaroslaw Maciaczyk, Gregor Bara, Hartmut Vatter, Mohammed Banat, Motaz Hamed
Summary: This study compared the efficacy of frameless biopsy using VarioGuide and frame-based biopsy using the Leksell stereotactic system (LSS) in brain biopsy surgeries. The results showed that VarioGuide biopsies took less time than LSS biopsies, with no significant differences in complication rates and lesion sizes between the two groups. Therefore, both systems provided a high degree of patient safety.
Article
Cell & Tissue Engineering
Ria Thomas, Vishal Menon, Rakesh Mani, Jan Pruszak
Summary: The neural crest gives rise to a diverse range of cell types, and studying their development can provide insights into neurocristopathies, neural cancer biology, and regenerative medicine. By culturing neural crest cells derived from stem cells, researchers have identified CD surface markers that define different stages of human neural crest development. These markers can be used to study neural crest stemness, epithelial-to-mesenchymal transition (EMT), and malignancies.
STEM CELL REVIEWS AND REPORTS
(2022)
Article
Chemistry, Medicinal
Julia Tsiampali, Silke Neumann, Beatriz Giesen, Katharina Koch, Donata Maciaczyk, Christoph Janiak, Daniel Haenggi, Jaroslaw Maciaczyk
Letter
Anesthesiology
Gregor A. Bara, Dirk de Ridder, Hartmut Vatter, Jaroslaw Maciaczyk
Letter
Medicine, Research & Experimental
Gregor A. Bara, Jaroslaw Maciaczyk