Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Cell & Tissue Engineering
Lee Chuen Liew, Boon Min Poh, Omer An, Beatrice Xuan Ho, Christina Ying Yan Lim, Jeremy Kah Sheng Pang, Leslie Y. Beh, Henry He Yang, Boon-Seng Soh
Summary: This study successfully identified cell surface markers for the purification of cardiac subtype cardiomyocytes, which provides an important foundation for cardiac disease modeling, drug discovery, and regenerative medicine.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biology
Chengyan Chen, Yanmin Shen, Luqian Li, Yaoxin Ren, Zhao-Qi Wang, Tangliang Li
Summary: Nonsense-mediated mRNA decay (NMD) is a highly conserved regulatory mechanism in eukaryotic cells, and UPF3A and UPF3B are key factors in this process. This study found that UPF3A does not repress or promote NMD in mouse embryonic stem cells, somatic cells, and major organs when UPF3B is present.
LIFE SCIENCE ALLIANCE
(2023)
Review
Genetics & Heredity
Xuepeng Wang, Qiang Wu
Summary: This review summarizes the three pluripotent states of PSCs, their features, and the underlying molecular mechanisms that maintain their identities. The interrelation and transition among these states are also discussed. Understanding the divergence of pluripotent states is crucial for fully harnessing the potential of stem cells in regenerative medicine.
Article
Cell & Tissue Engineering
Wei Shan, Qin Yu, Yan Long, Qian Luo, Honghu Li, Yingli Han, Yulin Xu, Shan Fu, Xiangjun Zeng, Cong Wei, Yang Gao, Xiaoqing Li, Xia Li, Lifei Zhang, Lizhen Liu, Ming Chen, Pengxu Qian, He Huang
Summary: This study established an in vitro differentiation system that successfully generated hematopoietic cells with in vivo hematopoietic reconstitution potential from mouse PSCs. The combination of 3D induction system and OP9 stromal cell coculture yielded c-kit(+) cells with superior repopulating activity.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Takashi Yoshino, Takahiro Suzuki, Go Nagamatsu, Haruka Yabukami, Mika Ikegaya, Mami Kishima, Haruka Kita, Takuya Imamura, Kinichi Nakashima, Ryuichi Nishinakamura, Makoto Tachibana, Miki Inoue, Yuichi Shima, Ken-ichirou Morohashi, Katsuhiko Hayashi
Summary: Novel methods have been developed to generate functional mouse oocytes from pluripotent stem cell-derived primordial germ cell-like cells (PGCLCs) and fetal ovarian somatic cell-like cells (FOSLCs). When FOSLCs are aggregated with PGCLCs, they enter meiosis and produce oocytes capable of fertilization and development into live offspring, providing a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Daehwan Kim, Sangho Roh
Summary: Stem cell research is crucial for studying human diseases, genetic preservation, and improving animals. Efforts have been made to establish true ESCs in various species, although capturing true bESCs in cattle remains a challenge. This review discusses the difficulties in establishing bESCs with different culture conditions and introduces alternative induced pluripotent stem cells and extended pluripotent stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xinchao Miao, Kunimichi Niibe, Maolin Zhang, Zeni Liu, Praphawi Nattasit, Yumi Ohori-Morita, Takashi Nakamura, Xinquan Jiang, Hiroshi Egusa
Summary: Amelogenin is a major component of enamel proteins, but the role of Amelx transcriptional activation in regulating ameloblast differentiation from iPSCs is unclear. This study induced Amelx expression in mouse iPSCs and used a three-stage strategy to differentiate cells into ameloblast lineage. Lithium chloride was found to enhance differentiation of dental epithelial cells, leading to the formation of ameloblast lineage with specific markers and calcium deposition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Priscila Anhel Medrano-Gonzalezl, Franklin Cruz-Villegas, Alejandro Alarcon del Carmen, Luis Felipe Montano, Erika Patricia Rendon-Huerta
Summary: Claudin-6 overexpression is associated with an increase in the generation of cancer stem cells and dedifferentiation of epithelial cells in gastric cancer.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Menno ter Huurne, Hendrik G. Stunnenberg
Summary: During early embryonic development, the rapid increase in cell number and the expression of genes that control developmental decisions are tightly regulated and interconnected processes. Transcription factors and signaling pathways play a crucial role in maintaining pluripotency and cell cycle progression, while canonical cell cycle regulators impact molecular networks controlling pluripotency and lineage specification.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yuko Nagai, Hiromi Nakao, Aya Kojima, Yuka Komatsubara, Yuki Ohta, Nana Kawasaki, Nobuko Kawasaki, Hidenao Toyoda, Toshisuke Kawasaki
Summary: The study developed two glycan-recognizing antibodies, R-10G and R-17F, using hiPSC/hESC lines as antigens. By investigating different cell lines, they found shared keratan sulfate epitopes in hiPSCs, with variations in the composition of keratan sulfate between different cell lines.
Review
Cell & Tissue Engineering
Chunmeng Yao, Ruqiang Yao, Haining Luo, Ling Shuai
Summary: This review describes in vitro germline specification studies, focusing on the reconstitution of PGCs, meiosis, signaling pathways, and factors involved in these processes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Genetics & Heredity
Huajin Li, Wenting Xu, Sijia Xiang, Leiting Tao, Wen Fu, Jinhui Liu, Wenbin Liu, Yamei Xiao, Liangyue Peng
Summary: Pluripotency is a transient state in early embryos regulated by a network of pluripotency-related genes. This study identified four pluripotent genes in zebrafish and crucian carp and verified that three of them can be used as markers for pluripotency in induced pluripotent stem cells in fish.
FRONTIERS IN GENETICS
(2022)
Article
Oncology
Yihang Shen, Yuan-Hao Lee, Jayabal Panneerselvam, Jun Zhang, Lenora W. M. Loo, Peiwen Fei
Article
Pathology
Taotao Dong, Dong Yang, Rui Li, Lu Zhang, Hongchao Zhao, Yihang Shen, Xiaoli Zhang, Beihua Kong, Linlin Wang
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2016)
Article
Oncology
Bing Han, Yihang Shen, Piyan Zhang, Panneerselvam Jayabal, Raymond Che, Jun Zhang, Herbert Yu, Peiwen Fei
Article
Oncology
Shasha Zhou, Yihang Shen, Min Zheng, Linlin Wang, Raymond Che, Wanning Hu, Pin Li
Article
Oncology
Linlin Wang, Dong Yang, Jing Tian, Aiqin Gao, Yihang Shen, Xia Ren, Xia Li, Guosheng Jiang, Taotao Dong
Article
Multidisciplinary Sciences
Panneerselvam Jayabal, Chi Ma, Manoj Nepal, Yihang Shen, Raymond Che, James Turkson, Peiwen Fei
SCIENTIFIC REPORTS
(2017)
Article
Cell Biology
Chi Ma, Kanani Hokutan, Yihang Shen, Manoj Nepal, Jin-Hee Kim, Jun Zhang, Peiwen Fei
Article
Genetics & Heredity
Yihang Shen, Shasha Zhou, Xiaodong Zhao, Hua Li, Jielin Sun
FRONTIERS IN GENETICS
(2020)
Article
Medicine, Research & Experimental
Shasha Zhou, Yihang Shen, Shaolian Zang, Xiaoqin Yin, Pin Li
Summary: In this study, HTR1A antagonists were found to enhance GnRH transcription by promoting PRC1 degradation and H2AK119ub loss through reduced CBX4 expression via suppression of the PI3K/Akt and MAPK/ERK pathways in GT1-7 cells, potentially providing an epigenetic mechanism of action of HTR1A on GnRH gene expression for mammalian puberty onset.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Shasha Zhou, Shaolian Zang, Yanping Hu, Yifen Shen, Hua Li, Wenlian Chen, Pin Li, Yihang Shen
Summary: This study investigated the gene expression patterns in different pubertal stages in the hypothalamus and identified a subset of kisspeptin neurons using a specific membrane surface biomarker. It was also found that different cell types in the hypothalamus have distinct functions in neurogenesis, hormone secretion, and estradiol response, contributing to the complex regulatory network of the hypothalamic-pituitary-gonadal axis.
CELL AND BIOSCIENCE
(2022)
Article
Medicine, Research & Experimental
Yihang Shen, Hongchao Zhao, Lei Zhang, Yanping Hu, Li Cai, Jun Li, Shasha Zhou
Summary: The study revealed that changes in DNA hydroxymethylation and DNA methylation were associated with gene expression patterns during puberty in the arcuate nucleus, with SINEs playing a regulatory role in gene expression.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Cell Biology
Yanping Hu, Yifen Shen, Yang Zhao, Ying Tang, Chao Liu, Yongchun Gu, Tao Yang, Yihang Shen
Summary: In this study, ChIP-seq was used to investigate the genome-wide distribution of activated STAT proteins in colorectal cancer cells. It was found that the homodimers of STAT proteins predominantly occupied genomic DNA and STAT3 accounted for the largest proportion. The binding motif targeted by different STAT homodimers suggested distinct biological functions. The study also revealed NR5A2 as a specific co-activator of STAT3 and its role in CRC. These findings provide new insights into the design of STAT inhibitors for treating CRC and other diseases.
Article
Immunology
Yanping Hu, Jiuzhou Zhao, Yihang Shen, Chengjuan Zhang, Qingxin Xia, Guoqiang Zhang, Bo Wang, Bing Wei, Rentao Yu, Jie Ma, Yongjun Guo
Summary: The study found that CD8 + CD279+ cells are an independent prognostic factor for DFS in CRC patients, with high levels of CD8 + CD279+ cells associated with better DFS.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
