Article
Multidisciplinary Sciences
Zhuoyi Liang, Vipul Kumar, Marie Le Bouteiller, Jeffrey Zurita, Josefin Kenrick, Sherry G. Lin, Jiangman Lou, Jianqiao Hu, Adam Yongxin Ye, Cristian Boboila, Frederick W. Alt, Richard L. Frock
Summary: The study demonstrates that in Lig4-deficient G1-arrested progenitor B cells, Ku-dependent blockage of A-EJ leads to functional endjoining suppression. However, joining of RAG-generated DSBs in Ku70-deficient or Ku70/Lig4 double-deficient cells occurs through a translocation-like A-EJ mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Menno ter Huurne, Hendrik G. Stunnenberg
Summary: During early embryonic development, the rapid increase in cell number and the expression of genes that control developmental decisions are tightly regulated and interconnected processes. Transcription factors and signaling pathways play a crucial role in maintaining pluripotency and cell cycle progression, while canonical cell cycle regulators impact molecular networks controlling pluripotency and lineage specification.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Clivia Lisowski, Jane Dias, Susana Costa, Ricardo Jorge Silva, Miguel Mano, Ana Eulalio
Summary: Research indicates that during Salmonella infection, dysregulation of host cell endolysosomal trafficking leads to inhibition of vacuolar replication in host cells arrested in G(1), while cytosolic replication of the closely related pathogen Shigella remains unaffected. This disruption results in Salmonella release into the host cytosol, compromising vacuole maturation and integrity.
Article
Biochemistry & Molecular Biology
Po-Hsuen Lee, Mary Ann Osley
Summary: A study revealed that quiescent cells undergo regulated DNA replication when resuming growth, with low levels of replication initiation factors requiring resynthesis, delaying entry into S phase. Fewer replication origins were activated in quiescent cells, with differences in chromatin structure compared to G1 cells.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Engineering, Chemical
Jing Tan, Chen Yang, Wensheng Deng, Lai Chen, Yuefeng Su
Summary: Recycling valuable metals from spent Li-ion batteries effectively alleviates metal resource shortage and prevents harm to human health and the environment. This study used D2EHPA/TBP/sulfonated kerosene as the extractant and intensification methods to conduct reactive extraction of Mn. The mass transfer performance was investigated, and optimal structures and operating intervals were recommended. Mathematical models were also established. This study provided simple and effective methods for process intensification in recycling valuable metals from spent Li-ion batteries with viscous extractants.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Gehan Safwat, Esraa S. M. Soliman, Hanan R. H. Mohamed
Summary: This study evaluated the therapeutic potential of erbium oxide nanoparticles (Er2O3-NPs) on human hepatocellular carcinoma (Hep-G2) cells. Exposure to Er2O3-NPs inhibited the growth of Hep-G2 cells and induced cell death in a concentration dependent manner. Er2O3-NPs also caused high levels of DNA damage and intracellular reactive oxygen species (ROS) production in Hep-G2 cells. Flow cytometry analysis revealed that Er2O3-NPs arrested the cell cycle of Hep-G2 cells at the G0/G1 phase and increased the number of cells in the apoptotic and necrotic phases. Furthermore, Er2O3-NPs upregulated the expression levels of apoptotic genes (p53 and Bax) and downregulated the expression level of the anti-apoptotic gene Bcl2 in Hep-G2 cells. In conclusion, Er2O3-NPs inhibit the proliferation of Hep-G2 cells and induce apoptosis through ROS generation, DNA damage, and alteration of apoptotic genes. Further in vitro and in vivo studies are recommended to explore the potential of using Er2O3-NPs in cancer treatment.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Pongtai Chaiputtanapun, Kriengsak Lirdprapamongkol, Bongkotrat Thanaussavadate, Thanyaporn Phongphankhum, Thanawit Thippong, Poomsith Thangsan, Phreeranat Montatip, Lukana Ngiwsara, Jisnuson Svasti, Pitak Chuawong
Summary: A series of compounds synthesized by Larock heteroannulation showed good cytotoxic activity against human lung cancer cells, through inhibition of tubulin polymerization, modulation of cell cycle, and downregulation of apoptosis-related proteins.
Article
Chemistry, Physical
Xiaoyan Zhang, Wei Zhou, Yang Liu, Linyu Jin, Jiawei Huo, Yang Yang, Shumu Li, Haijun Ma, Jiao Li, Mingming Zhen, Jie Li, Chunru Wang
Summary: Functional fullerene derivatives have shown inhibitory effects on tumor progress and metastasis by regulating tumor microenvironment, but the molecular mechanisms remain unknown. This study demonstrates that nanosize aminated fullerene can activate autophagic flux, induce G0/G1 cell cycle arrest, and inhibit tumor growth by promoting the expression of cathepsin D. This work reveals the anti-tumor activity of aminated fullerene, elucidates the molecular mechanism, and provides a new strategy for antineoplastic drug development.
Article
Environmental Sciences
Bai Li, Cunye Qiao, Xiaolei Jin, Hing Man Chan
Summary: The study illustrated that exposure to low doses of MeHg induced apoptosis in some cells while enhancing the expression of self-renewal genes in others. This suggests that low doses of MeHg adversely affect hESCs during embryonic development stages, with a potential for both cell loss and adaptation.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2021)
Article
Biochemistry & Molecular Biology
Yong-Li Zhang, Gui-Lin Chen, Ye Liu, Xiao-Cui Zhuang, Ming-Quan Guo
Summary: The study demonstrated that the extract of Warburgia ugandensis Sprague (WUD) exhibited significant anti-proliferative effects on A549 cells by increasing intracellular ROS levels and subsequently modulating cell proliferation and G(0)/G(1) cell cycle progression.
Article
Oncology
Yoshifumi Ohno, Ruirong Yi, Akiko Suganami, Yutaka Tamura, Akio Matsumoto, Shoji Matsumoto, Kengo Saito, Hiroshi Shirasawa
Summary: CCL299 exhibits cytotoxic activity via apoptosis in a subset of cancer cells, while showing no significant cytotoxic effects on noncancer cells.
ANTICANCER RESEARCH
(2021)
Article
Oncology
Su Su Thae Hnit, Mu Yao, Chanlu Xie, Ling Bi, Matthew Wong, Tao Liu, Paul De Souza, Zhong Li, Qihan Dong
Summary: Apigenin, a natural flavone found in various foods, has been shown to inhibit proliferation of prostate cancer cells by arresting them at the G(2) phase. It also reduces the expression of key regulators involved in G(2)-M transition. This study provides mechanistic insights into the inhibitory effect of apigenin on cancer cell growth and supports its potential as an anti-cancer agent.
Article
Plant Sciences
Asmita Pal, Soumee Sengupta, Rita Kundu
Summary: This study confirms the ethnopharmacological anticancer role of T. racemosa, inhibiting the abnormal proliferation of cervical cancer cells SiHa by inducing G2/M-phase cell cycle arrest and apoptotic cell death. Oxidative stress-mediated double-stranded DNA damage leads to apoptotic cell death through multiple routes, including the PI3K/Akt/NFKB pathway. The abundant alkaloid content of T. racemosa is suggested as the probable responsible cytotoxic principle.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Food Science & Technology
Daniel Pleissner, Sergiy Smetana
Summary: Microalgae cell walls have potential applications in separation chromatography, but further experimental proof is needed.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2022)
Article
Developmental Biology
Angie Molina, Frederic Bonnet, Julie Pignolet, Valerie Lobjois, Sophie Bel-Vialar, Jacques Gautrais, Fabienne Pituello, Eric Agius
Summary: Although the lengthening of cell cycle and G1 phase is a common phenomenon during tissue maturation, the underlying mechanism is still unclear. This study developed a time-lapse imaging strategy to measure the four cell cycle phases in single chick neural progenitor cells, and found that neural progenitors exhibit wide heterogeneity in cell cycle length. The study also identified a mechanism that contributes to the lengthening of G1 phase.