Article
Cell Biology
Ting Zhao, Shengfan Ye, Zimu Tang, Liwei Guo, Zhipeng Ma, Yuxi Zhang, Chun Yang, Jinrong Peng, Jun Chen
Summary: Delta 133p53 plays a protective role against cellular senescence by upregulating antioxidant genes expression, and its decreased expression in brain tissues of AD and ALS patients has been observed. The zebrafish ortholog Delta 113p53 contributes to neurogenesis and brain function maintenance, with loss of cognitive ability observed in mutants but not in wild-type fish at older ages.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Alexandra N. Boix De Jesus, Ahmad Taha, David Wang, Paulomi M. M. Mehta, Sunali Mehta, Ashley Reily-Bell, Sasini Polwatta Lekamlage, Adriana Machado Saraiva, Tahmeed Tahmeedzaman, Fouzia Ziad, Ziad Thotathil, Peter Y. C. Gan, Janice Royds, Antony Braithwaite, Noelyn Hung, Tania L. L. Slatter
Summary: The expression of delta 133p53 beta is increased in various primary tumors and is associated with increased proliferation, migration, and inflammation. In this study, it was found that delta 133p53 beta expression is also related to the development and survival of brain metastases. Furthermore, delta 133p53 beta-expressing cells have increased cancer-promoting properties and invasiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Xiajie Zhang, Kira Groen, Brianna C. Morten, Luiza Steffens Reinhardt, Hamish G. Campbell, Antony W. Braithwaite, Jean-Christophe Bourdon, Kelly A. Avery-Kiejda
Summary: This study aimed to evaluate the role of Delta 40p53 in breast cancer migration and invasion, finding an association between Delta 40p53 and gene expression profiles. Knockdown of both p53 alpha and Delta 40p53 resulted in increased proliferation, while overexpression of Delta 40p53 reduced proliferation rates and decreased migratory and invasive properties of the cells. These results suggest that at a basal level, Delta 40p53 works similarly to p53 alpha in suppressing cellular mobility and proliferation.
MOLECULAR ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Guanglin Niu, Isabel Hellmuth, Tatiana Flisikowska, Hubert Pausch, Beate Rieblinger, Alexander Carrapeiro, Benjamin Schade, Brigitte Boehm, Eva Kappe, Konrad Fischer, Bernhard Klinger, Katja Steiger, Reiner Burgkart, Jean-Christophe Bourdon, Dieter Saur, Alexander Kind, Angelika Schnieke, Krzysztof Flisikowski
Summary: The mutation in TP53 gene in pig models leads to the development of tumors like osteosarcoma, pointing out similarities with human TP53. Specific TP53 isoforms determine the tumor spectrum in pigs, with circTP53 playing a critical role in malignant transformation.
Article
Biochemistry & Molecular Biology
XiaoDong Jin, WenYe Liu, Jing Miao, ZhiPeng Tai, LingYa Li, PengPeng Guan, Jing-Xia Liu
Summary: The study shows that disrupted Cu2+ homeostasis can lead to developmental defects in zebrafish myogenesis, possibly through regulating gene promoter methylation and histone modification to affect signaling transduction pathways.
Article
Fisheries
Xin Zhao, Wenji Huang, Jiahong Guo, Ning Ji, Jianhua Feng, Kangyong Chen, Jun Zou, Jun Zou
Summary: The study found that PLAAT1 is involved in the interplay between p53 mediated cellular responses and autophagy.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Suleyman Vural, Lun-Ching Chang, Laura M. Yee, Dmitriy Sonkin
Summary: TP53 is frequently mutated in cancer and can be inactivated by multiple mechanisms. Despite the expectation of increased expression of TP53 C-terminal alternative isoforms in tumors with wild type TP53, no substantial increase was observed in this study, suggesting that these isoforms may not be reliably selected for during tumor progression.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Sunali Mehta, Hamish Campbell, Catherine J. Drummond, Kunyu Li, Kaisha Murray, Tania Slatter, Jean-Christophe Bourdon, Antony W. Braithwaite
Summary: p53 is a crucial protein in maintaining biological homeostasis, with its network playing a vital role in maintaining stability within multicellular organisms, facilitating cooperation between cells to adapt to environmental changes.
Article
Cell Biology
Aanchal Katoch, Sachin Kumar Tripathi, Apala Pal, Saumitra Das
Summary: The study reveals an antagonistic regulation between 40p53 and miR-186-5p, with 40p53 affecting cell proliferation through its regulation of miR-186-5p and YY1. The results demonstrate an interdependence among 40p53, miR-186-5p, YY1, and cell proliferation.
Review
Cell Biology
Kim Kobar, Keon Collett, Sergey V. Prykhozhij, Jason N. Berman
Summary: Cancer predisposition syndromes, typically rare monogenic disorders caused by germline mutations, increase the likelihood of developing cancer. The zebrafish has emerged as a powerful model for cancer research, supporting large-scale genetic and drug screening, and aiding in the understanding of tumor-driving mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria Jose Lopez-Iniesta, Shrutee N. Parkar, Ana Catarina Ramalho, Rafaela Lacerda, Ines F. Costa, Jingyuan Zhao, Luisa Romao, Marco M. Candeias
Summary: p53 is the most commonly mutated gene in human cancers. It has been found that the long protein isoforms of p53 can protect against cancer, while the shorter C-terminal isoforms can support cancer and metastasis. The Δ160p53 protein isoform has been shown to enhance survival and invasive characteristics of cancer cells. In this study, a translation initiation site was identified downstream of codon 160, and when translation failed to initiate from AUG160 due to mutation, it initiated from AUG169 instead, producing a similar protein, Δ169p53, which promotes cell survival following DNA damage. The presence of both initiation sites is more common in mammalian species, suggesting a potential role in essential protein expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nikola Arsic, Tania Slatter, Gilles Gadea, Etienne Villain, Aurelie Fournet, Marina Kazantseva, Frederic Allemand, Nathalie Sibille, Martial Seveno, Sylvain de Rossi, Sunali Mehta, Serge Urbach, Jean-Christophe Bourdon, Pau Bernado, Andrey Kajava, Antony Braithwaite, Pierre Roux
Summary: The p53 isoform Delta 133p53 beta promotes intrinsic oncogenic functions, with its activity regulated through an aggregation-dependent mechanism. Interaction with partners like p63 family members or the CCT chaperone complex influences cancer cell features such as migration and invasion by modulating Delta 133p53 beta activity.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yan Sun, Ambroise Manceau, Lisa Frydman, Lucie Cappuccio, David Neves, Valeria Basso, Hong Wang, Joanna Fombonne, Carine Maisse, Patrick Mehlen, Andrea Paradisi
Summary: Netrin-1, a secreted protein known as a relevant cancer therapeutic target, promotes cancer progression by inhibiting cell death induced by its receptors. The study shows that p53 positively regulates Netrin-1 gene expression, and the A40p53 isoform can activate the Netrin-1 promoter to promote cell survival.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Jeong-Ryul Hwang, Donghwan Kim, Jung-Ah Kang, Sang-Heon Park, Sung-Gyoo Park
Summary: Notch1 plays important roles in T cell development and is highly expressed in activated CD4(+) T cells. The transcription of Notch1 in activated CD4(+) T cells is upregulated via the PI3K-PDK1-NF-kappa B signaling pathway. The -300 to -270 region of the Notch1 promoter is crucial for its transcription in activated T cells.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Lu Cao, Dong Yan, Jun Xiao, Hao Feng, Ming Xian Chang
Summary: This study demonstrates the functional characteristics of BIRC2 in zebrafish in response to Edwardsiella piscicida infection, showing its negative regulation on antibacterial immune response by inhibiting caspases and accumulating p53.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Xin Ting Zheng, Walter L. Goh, Peter Yeow, David P. Lane, Farid J. Ghadessy, Yen Nee Tan
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Biochemistry & Molecular Biology
Barindra Sana, Sharon M. Q. Chee, Jantana Wongsantichon, Sarada Raghavan, Robert C. Robinson, Farid J. Ghadessy
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Sarada S. Raghavan, Sharon Chee, Juntao Li, Jeremie Poschmann, Niranjan Nagarajan, Siau Jia Wei, Chandra S. Verma, Farid J. Ghadessy
MICROBIAL CELL FACTORIES
(2019)
Article
Biochemistry & Molecular Biology
Jia Wei Siau, Samuel Nonis, Sharon Chee, Li Quan Koh, Fernando J. Ferrer, Christopher J. Brown, Farid J. Ghadessy
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Sharon Min Qi Chee, Jantana Wongsantichon, Lau Sze Yi, Barindra Sana, Yuri Frosi, Robert C. Robinson, Farid J. Ghadessy
Summary: This study demonstrates the use of vGFP as a scaffold for displaying peptides, showing that peptides correctly inserted in the linker region can interact with targets both in vitro and in live cells. The thermostable and inherently fluorescent properties of vGFP make it a promising tool for disrupting clinically relevant protein-protein interactions.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Barindra Sana, Timothy Ho, Srinivasaraghavan Kannan, Ding Ke, Eunice H. Y. Li, Jayasree Seayad, Chandra S. Verma, Hung A. Duong, Farid J. Ghadessy
Summary: Engineering tryptophan halogenase enzymes can efficiently halogenate various indole substrates and produce important pharmaceutical intermediates. The engineered enzymes exhibit high tolerance towards indole derivatives, but are completely inactive towards their natural substrate tryptophan.
Article
Oncology
Bibek Bhatta, Ishai Luz, Christian Krueger, Fanny Xueting Teo, David P. Lane, Kanaga Sabapathy, Tomer Cooks
Summary: Extracellular vesicles (EVs) released by cancer cells play a crucial role in transferring molecular information and reprogramming the tumor microenvironment. Mutations in the TP53 gene, encoding the p53 protein, lead to loss of tumor-suppressive properties and acquisition of novel functions that promote cancer growth. This study demonstrates that mutant p53 proteins can be transferred via EVs to neighboring cancer cells and immune cells, modulating them to support tumor growth.
Article
Chemistry, Multidisciplinary
Yue Sun, Sze Yi Lau, Zhi Wei Lim, Shi Chieh Chang, Farid Ghadessy, Anthony Partridge, Ali Miserez
Summary: This study reports the development of conjugated peptides that can form coacervate microdroplets through liquid-liquid phase separation. These microdroplets have the ability to easily cross the cell membrane and can recruit and release macromolecular therapeutic agents, making them promising for treating various diseases.
Article
Multidisciplinary Sciences
Samira Sadeghi, Siddharth Deshpande, Girish Vallerinteavide Mavelli, Alphan Aksoyoglu, Jayesh Bafna, Mathias Winterhalter, R. Manjunatha Kini, David P. Lane, Chester L. Drum
Summary: In vitro protein folding often leads to aggregation and low yields. Researchers have used nanoscale exoshells to enhance soluble yield, functional yield, and specific activity of folded proteins.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Margit Kaldmae, Thibault Vosselman, Xueying Zhong, Dilraj Lama, Gefei Chen, Mihkel Saluri, Nina Kronqvist, Jia Wei Siau, Aik Seng Ng, Farid J. Ghadessy, Pierre Sabatier, Borivoj Vojtesek, Medoune Sarr, Cagla Sahin, Nicklas Osterlund, Leopold L. Ilag, Venla A. Vaananen, Saikiran Sedimbi, Marie Arsenian-Henriksson, Roman A. Zubarev, Lennart Nilsson, Philip J. B. Koeck, Anna Rising, Axel Abelein, Nicolas Fritz, Jan Johansson, David P. Lane, Michael Landreh
Summary: Disordered proteins pose challenges to structural biology. Researchers have found that fusing the protein with a highly soluble spider silk domain can modulate its characteristics and enhance translation efficiency. This mechanism is of great significance for cancer treatment.
Editorial Material
Oncology
David P. Lane, Chandra S. Verma
Summary: Mutant p53 proteins are found in various human cancers. In this study, Guiley and Shokat report on the development of compounds that can restore the function of Y220C mutant p53 protein by covalently binding to it.
Article
Biotechnology & Applied Microbiology
Eunice Hui Yen Hui, Barindra Sana, Timothy Ho, Ding Ke, Farid J. Ghadessy, Hung A. Duong, Jayasree Seayad
Summary: The study introduces a novel enzymatic halogenation method, which can efficiently carry out halogenation of industrially important compounds without the need for external enzymes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Barindra Sana, Ding Ke, Eunice Hui Yen Li, Timothy Ho, Jayasree Seayad, Hung A. Duong, Farid J. Ghadessy
Summary: Halogenation of peptides can be achieved through selective halogenase enzymes, providing potential for post-synthesis/expression halogenation. The C-terminal (G/S)GW motif is identified as the preferred substrate. Improved halogenation of the (G/S)GW motif can be achieved through rational design of PyrH halogenase mutants.
Article
Multidisciplinary Sciences
Yuezhen Xue, Boris San Luis, Regine J. Dress, Katzrin Binte Ahmad Murad, Florent Ginhoux, Nick Barker, David Lane
Summary: This study demonstrates that the proteasome inhibitor bortezomib stabilizes p53 in stem and progenitor cells in gastrointestinal and lymphoid tissues. The stabilization of p53 is observed in various hematopoietic stem and progenitor cells, as well as T cells and B cells. Understanding the regulation of p53 in these cells is important for developing strategies for cancer treatment and immunotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Yancen Dai, Yuko Sato, Bo Zhu, Tetsuya Kitaguchi, Hiroshi Kimura, Farid J. Ghadessy, Hiroshi Ueda
Summary: This study developed a conditionally active Q-body probe for precise imaging of intracellular p53 in living cells with low background signal. The Q-body showed significant fluorescence enhancement in vitro and in both fixed and live cells. Live-cell sorting based on p53 expression was also demonstrated.