Article
Multidisciplinary Sciences
Koen Debackere, Lukas Marcelis, Sofie Demeyer, Marlies Vanden Bempt, Nicole Mentens, Olga Gielen, Kris Jacobs, Michael Broux, Gregor Verhoef, Lucienne Michaux, Carlos Graux, Iwona Wlodarska, Philippe Gaulard, Laurence de Leval, Thomas Tousseyn, Jan Cools, Daan Dierickx
Summary: This study identifies two fusion transcripts that activate T cell receptor complex signaling and confer therapeutic vulnerability in PTCL-NOS, contributing to the understanding of this poorly characterized subgroup of PTCL at the genetic level.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Jordan F. Hastings, Sharissa L. Latham, Alvin Kamili, Madeleine S. Wheatley, Jeremy Z. R. Han, Marie Wong-Erasmus, Monica Phimmachanh, Max Nobis, Chiara Pantarelli, Antonia L. Cadell, Yolande E. I. O'Donnell, King Ho Leong, Sophie Lynn, Fan-Suo Geng, Lujing Cui, Sabrina Yan, Joanna Achinger-Kawecka, Clare Stirzaker, Murray D. Norris, Michelle Haber, Toby N. Trahair, Frank Speleman, Katleen De Preter, Mark J. Cowley, Ozren Bogdanovic, Paul Timpson, Thomas R. Cox, Walter Kolch, Jamie I. Fletcher, Dirk Fey, David R. Croucher
Summary: Gene expression noise promotes stochastic drug resistance in rare cancer cells. However, when integrated across multiple components of an apoptotic signaling network, the influence of noise leads to a higher frequency of chemoresistant neuroblastoma cells. These cells are characterized by JNK impairment and retain a memory of their resistant state even after chemotherapy treatment.
Article
Multidisciplinary Sciences
Xiangjun Liu, Shanzhao Jin, Simeng Hu, Ruoyan Li, Haihao Pan, Yi Liu, Pan Lai, Deshu Xu, Jingru Sun, Ziyang Liu, Yumei Gao, Yifan Zhao, Fengjie Liu, Yu Xiao, Yingyi Li, Yujie Wen, Zhuojing Chen, Bufang Xu, Yuchieh Lin, Menglong Ran, Qianxi Li, Shuxia Yang, Hang Li, Ping Tu, Muzlifah Haniffa, Sarah A. Teichmann, Fan Bai, Yang Wang
Summary: Cutaneous T cell lymphoma (CTCL) is a heterogeneous group of non-Hodgkin lymphoma characterized by clonal malignant T cells. This study analyzed CTCL patient samples using single-cell RNA-seq, TCR and whole-exome sequencing, revealing the molecular profiles of malignant T cells and their association with the microenvironment and clinical outcomes.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Andrea C. Pichler, Nadege Carrie, Marine Cuisinier, Samira Ghazali, Allison Voisin, Pierre-Paul Axisa, Marie Tosolini, Celine Mazzotti, Dominic P. Golec, Sabrina Maheo, Laura do Souto, Ruchan Ekren, Eve Blanquart, Lea Lemaitre, Virginie Feliu, Marie-Veronique Joubert, Jennifer L. Cannons, Camille Guillerey, Herve Avet-Loiseau, Tania H. Watts, Benoit L. Salomon, Olivier Joffre, Yenkel Grinberg-Bleyer, Pamela L. Schwartzberg, Liliana E. Lucca, Ludovic Martinet
Summary: CD137 (4-1BB) is a promising target for cancer immunotherapy. It modulates the infiltration of CD8+ exhausted T cells expressing inhibitory receptors into tumors. CD137 signaling stimulates the proliferation and differentiation of Tex precursor cells through NF-KB subunits RelA and cRel and Tox-dependent chromatin remodeling. Understanding T cell exhaustion is crucial for cancer and infectious disease treatment. CD137 is identified as a critical regulator of Tex cell expansion and differentiation with broad therapeutic potential.
Article
Cell Biology
Mette Trauelsen, Thomas K. Hiron, Da Lin, Jacob E. Petersen, Billy Breton, Anna Sofie Husted, Siv A. Hjorth, Asuka Inoue, Thomas M. Frimurer, Michel Bouvier, Chris A. O'Callaghan, Thue W. Schwartz
Summary: The study identifies that succinate regulates transcription of immune function genes by activating Gq signaling, affecting the phenotype of macrophages M2 and M1. This indicates that SUCNR1 acts as a transcriptional regulator in macrophages.
Article
Multidisciplinary Sciences
Ke Xu, Na Yin, Min Peng, Efstathios G. Stamatiades, Amy Shyu, Peng Li, Xian Zhang, Mytrang H. Do, Zhaoquan Wang, Kristelle J. Capistrano, Chun Chou, Andrew G. Levine, Alexander Y. Rudensky, Ming O. Li
Summary: The passage discusses the impact of infection on T cell immune response, highlighting the crucial role of glycolytic enzyme LDHA in regulating the PI3K-Akt-Foxo1 signaling pathway, a mechanism that serves as a key factor in controlling T cell immunity.
Article
Immunology
Tianzhuo Zhang, Qianwen Shi, Huining Gu, Biaoyi Yu, Sha Yin, Qing Ge, Xiaoning Mo, Xiaofeng Liu, Jing Huang
Summary: CCDC134 is a cytokine-like molecule that serves as a positive regulator of TCR-proximal signaling by interacting with CD3e and modulating its ubiquitination and degradation. T cell-specific deletion of Ccdc134 results in decreased mature CD4(+) and CD8(+) T cells, impaired T cell homeostasis, and attenuated response to TCR stimulation in vitro and in vivo. These findings highlight the importance of CCDC134 in T cell development and function, and its role in T cell-mediated inflammatory and antitumor responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Adam Gayoso, Zoe Steier, Romain Lopez, Jeffrey Regier, Kristopher L. Nazor, Aaron Streets, Nir Yosef
Summary: totalVI is a framework for end-to-end joint analysis of CITE-seq data which probabilistically represents data as a composite of biological and technical factors, providing a cohesive solution for common analysis tasks. It demonstrates strong performance in tasks such as dimensionality reduction, dataset integration, correlation estimation, and differential expression testing.
Article
Hematology
Yang Liu, Qi Chen, Hyun-Woo Jeong, Dong Han, Joerg Fabian, Hannes C. A. Drexler, Martin Stehling, Hans R. Schoeler, Ralf H. Adams
Summary: This study reveals that dopamine derived from sympathetic nerves directly controls the behavior of hematopoietic stem and progenitor cells through D-2 subfamily dopamine receptors. Dopamine signaling affects proliferation, frequency, and transplantation efficiency of HSPCs.
Article
Immunology
Zhi Liu, Xianting Hu, Yuqiong Liang, Jingting Yu, Huabin Li, Maxim N. Shokhirev, Ye Zheng
Summary: This study reveals the interaction between T-reg cells and HFSCs, which is mediated by the glucocorticoid receptor signaling pathway and TGF-beta 3, promoting HFSC proliferation and skin regeneration.
Article
Cell Biology
Longhui Zeng, Ivan Palaia, Andela Saric, Xiaolei Su
Summary: Phospholipase C gamma 1 (PLC gamma 1) promotes phase separation of LAT in the TCR pathway, directly cross-linking LAT through its two SH2 domains. PLC gamma 1 also protects LAT from dephosphorylation by CD45, and enhances LAT-dependent ERK activation and SLP76 phosphorylation. A nonmonotonic effect of PLC gamma 1 on LAT clustering was discovered, and computer simulations showed how cluster size is regulated by protein compositions. These results define a critical role of PLC gamma 1 in promoting phase separation of the LAT complex and TCR signal transduction.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Cell Biology
David A. Lewis, Tony Ly
Summary: CD8(+) T cells are crucial in immunity and immuno-oncology, as they undergo complex cellular growth, cell cycle entry, and differentiation to produce antigen-specific cytotoxic T lymphocytes. Some activated T cells will differentiate into memory T cells which are essential in adaptive immunity. This review explores the control of cell cycle entry in CD8(+) T cells and the interaction between these mechanisms and pathways regulating immunological phenotypes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Meagan R. Rollins, Jackson F. Raynor, Ebony A. Miller, Jonah Z. Butler, Ellen J. Spartz, Walker S. Lahr, Yun You, Adam L. Burrack, Branden S. Moriarity, Beau R. Webber, Ingunn M. Stromnes
Summary: TCR transgenic mice are a valuable tool for studying antigen-specific immune responses. The TRex methodology, which integrates TCRs specific to the self/tumor antigen mesothelin (Msln) into the Trac locus, is shown to improve the functional sensitivity of lower affinity TCRs and confer resistance to T cell functional loss. By comparing TCRs with the same specificity but different affinity, the Trac-targeted TCRs are shown to have increased avidity over transgenic TCRs while preserving physiological T cell development. Overall, the TRex methodology is an advanced tool for studying physiological antigen-specific T cell behavior.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Rebecca Newman, Pavel Tolar
Summary: In contrast to other antibody isotypes, B cells switched to IgE respond transiently and do not give rise to long-lived plasma cells (PCs) or memory B cells. IgE(+) PCs exhibit dependency on the PI3K-mTOR axis that increases protein amounts of the transcription factor IRF4, while loss of components of the calcium-calcineurin-NFAT pathway promotes IgE(+) PC differentiation.
Article
Biochemistry & Molecular Biology
Tommaso Colangelo, Patrizio Panelli, Francesco Mazzarelli, Francesco Tamiro, Valentina Melocchi, Elisabetta De Santis, Roberto Cuttano, Orazio Palumbo, Giovanni Rossi, Fabrizio Bianchi, Vincenzo Giambra
Summary: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive T-cell malignancy. Recent studies have shown that T-ALL-secreted small extracellular vesicles (sEV) contain NOTCH1-dependent microRNAs (EV-miRs), which regulate oncogenic pathways and promote the expansion and survival of highly proliferative cell subsets, thereby altering the cell heterogeneity and dynamics of leukemia.
Article
Reproductive Biology
Lu Sun, Rawad Hodeify, Shirley Haun, Amanda Charlesworth, Angus M. MacNicol, Subramaniam Ponnappan, Usha Ponnappan, Usha Ponnappan, Claude Prigent, Khaled Machaca
BIOLOGY OF REPRODUCTION
(2008)
Review
Cell Biology
Khaled Machaca
Review
Cell Biology
Nancy Nader, Rashmi P. Kulkarni, Maya Dib, Khaled Machaca
Article
Biochemistry & Molecular Biology
Shirley Haun, Lu Sun, Satanay Hubrack, David Yule, Khaled Machaca
Article
Developmental Biology
Wassim El-Jouni, Shirley Haun, Khaled Machaca
DEVELOPMENTAL BIOLOGY
(2008)
Meeting Abstract
Developmental Biology
Lu Sun, Fang Yu, Khaled Machaca
DEVELOPMENTAL BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Lu Sun, Shirley Haun, Richard C. Jones, Ricky D. Edmondson, Khaled Machaca
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Biochemistry & Molecular Biology
Fang Yu, Lu Sun, Raphael Courjaret, Khaled Machaca
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Cell Biology
Fang Yu, Lu Sun, Khaled Machaca
JOURNAL OF CELL BIOLOGY
(2010)
Article
Cell Biology
Abdelilah Arredouani, Fang Yu, Lu Sun, Khaled Machaca
JOURNAL OF CELL SCIENCE
(2010)
Article
Multidisciplinary Sciences
Lu Sun, Fang Yu, Aman Ullah, Satanay Hubrack, Arwa Daalis, Peter Jung, Khaled Machaca
Article
Multidisciplinary Sciences
Fang Yu, Lu Sun, Khaled Machaca
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2009)
Article
Cell Biology
Ghanim Ullah, Peter Jung, Khaled Machaca
Review
Cell Biology
Khaled Machaca
JOURNAL OF CELLULAR PHYSIOLOGY
(2007)
Article
Cell Biology
Dominic Tscherrig, Rajesh Bhardwaj, Daniel Biner, Jan Dernic, Daniela Ross-Kaschitza, Christine Peinelt, Matthias A. Hediger, Martin Lochner
Summary: Changes in Ca2+ levels regulate many physiological functions, and the endoplasmic reticulum is the major Ca2+ store in cells. Store-operated calcium entry (SOCE) is a refilling process that involves protein-protein interactions between Ca2+ sensing STIM in the ER and Orai proteins in the plasma membrane, forming selective Ca2+ channels. Abnormal SOCE is associated with diseases and cancers, making STIM and Orai important therapeutic targets. Novel GSK-7975A-based molecular probes have been developed for studying SOCE in different cells and settings.
Article
Cell Biology
Nicola Fameli, Cornelis van Breemen, Klaus Groschner
Summary: The study provides evidence for the importance of junctional membrane architecture in cellular function. Through modeling and prediction, it is shown that nano-scale membrane spacing plays a crucial role in junctional ER Ca2+ refilling and signaling efficiency. Additionally, interactions between Ca2+ and the phospholipid membrane surface are suggested to support interfacial Ca2+ transport and receptor targeting. This research introduces a new concept in pathophysiology.
Article
Cell Biology
Satomi Ohtsuka, Yumi Miyai, Hiroyuki Mima, Masaki Magari, Yoichi Chiba, Futoshi Suizu, Hiroyuki Sakagami, Masaki Ueno, Hiroshi Tokumitsu
Summary: This study characterized the splice variants of mouse CaMKKI3/2 and found that CaMKKI3-3 and I3-3x were functionally active and likely to be bona fide CaMKIV kinases involved in the regulation of spermiogenesis.