Article
Biochemistry & Molecular Biology
Nandini Mani, Shuo Jiang, Alex E. Neary, Sithara S. Wijeratne, Radhika Subramanian
Summary: The microtubule cytoskeleton features subpopulations with different dynamic properties, such as stable antiparallel bundles alongside dynamic microtubules like in the anaphase spindle. By reconstituting a system of three midzone proteins, researchers found that collective regulator activity can create microenvironments of arrays with distinct dynamic properties, promoting elongation of single microtubules while stalling polymerization of crosslinked bundles. This differentiation is due to the strong rescue activity of CLASP1 and the lower binding affinity of CLASP1, allowing Kif4A to dominate at overlaps.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Sithara S. Wijeratne, Shane A. Fiorenza, Alex E. Neary, Radhika Subramanian, Meredith D. Betterton
Summary: This study reports that microtubules can physically couple motor movement in the absence of detectable short-range interactions. The long-range microtubule-mediated coupling mechanism may affect the binding kinetics and mechanochemistry of motors, providing new forms of collective motor behavior.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Himanshu Pandey, Mary Popov, Alina Goldstein-Levitin, Larisa Gheber
Summary: Kinesin-5 motor proteins play crucial roles in cell division with their specialized structural features and diverse functions. Research has shown their different mechanisms of action in dividing and non-dividing cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Marta Urbanska, Annemarie Ludecke, Wilhelm J. Walter, Antoine M. van Oijen, Karl E. Duderstadt, Stefan Diez
Summary: This study introduces a highly-parallel, microfluidics-based method for rapid collection of force-dependent motility parameters of cytoskeletal motors, significantly improving throughput. DNA-tethered beads are used to apply tunable hydrodynamic forces to stepping kinesin-1 motors, allowing for simultaneous tracking of various motility parameters of hundreds of individual molecules. The approach, applicable to other molecular systems, represents a new methodology for parallelized single-molecule force studies on cytoskeletal motors.
Article
Biology
Rachel A. Banks, Vahe Galstyan, Heun Jin Lee, Soichi Hirokawa, Athena Ierokomos, Tyler D. Ross, Zev Bryant, Matt Thomson, Rob Phillips
Summary: Active matter systems generate ordered structures and avoid equilibrium through energy consumption. However, the translation of microscopic parameters to observed mesoscopic properties remains unclear. Investigating the motor-microtubule interactions, this research explores the impact of these interactions on coherent structures. The findings provide insights into the influence of microscopic motor properties on collective motor-microtubule assemblies.
Article
Multidisciplinary Sciences
Jacob Palumbo, Ellinor Tai, Scott Forth
Summary: Microtubule networks play important roles in cellular processes, but the biophysical regulation of crosslinking protein ensembles is not well understood. This study introduces a methodology for directly probing force generation and response within crosslinked microtubule minimal networks. By using optical techniques and fluorescence microscopy, the forces and dynamics of these networks can be visualized and studied. This research provides insights into spindle assembly and function, and can be extended to study microtubule network mechanics in different cellular contexts.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Microbiology
Jing Xu, Jie Gao, Maolin Zhang, Danwei Zhang, Ming Duan, Zhenhong Guan, Yidi Guo
Summary: Rabies virus infects N2a cells in a manner dependent on microtubule integrity as well as the function of dynein and kinesin. Microtubule-depolymerizing drugs, dynein inhibitors, and disruption of kinesin function can all hinder Rabies virus infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Alexander S. Zhovmer, Alexis Manning, Chynna Smith, James B. Hayes, Dylan T. Burnette, Jian Wang, Alexander X. Cartagena-Rivera, Nikolay V. Dokholyan, Rakesh K. Singh, Erdem D. Tabdanov
Summary: The study demonstrates an antagonistic mechanical balance within the dynein-kinesin microtubular motor system, where dynein activity drives inward compaction of the microtubular network, while kinesins bundle and expand MTs into giant circular bands. This balance controls the 3D architecture, mechanics, and cell-microenvironment interactions of cells through orthogonal mechanisms of MT network reorganization.
Article
Developmental Biology
Maelys Loh, Fred Bernard, Antoine Guichet
Summary: Microtubules and their associated motors are crucial for nucleus positioning, and Kinesin-1 is found to control nuclear migration in Drosophila oocytes by modulating centrosome activity.
Article
Biology
Julia R. Torvi, Jonathan Wong, Daniel Serwas, Amir Moayed, David G. Drubin, Georjana Barnes
Summary: During mitosis, microtubules make attachments to chromosomes via a protein complex called the kinetochore, facilitating accurate chromosome segregation. This study used TIRF microscopy to observe the translocation of kinetochores on the lateral surface of microtubules and found that it is dependent on the kinesin-8 protein Kip3. The findings provide insights into the mechanism of cell division.
Article
Cell Biology
Alexandra Ciorita, Michael Bugiel, Swathi Sudhakar, Erik Schaffer, Anita Jannasch
Summary: Studying the influence of single kinesin motors on the stability of microtubules in an in vitro assay, it was found that both Kip3 and nondepolymerizing kinesin-1 further stabilized microtubules. This finding may hint at a more general stabilization mechanism and provides new insights into the molecular mechanism and regulatory function of Kip3 on microtubule length.
Review
Cell Biology
Wen Lu, Vladimir I. Gelfand
Summary: Cells are the smallest building blocks of living organisms, and often undergo cytoplasmic streaming to compensate for physical constraints in large cells. This phenomenon is driven by molecular motors and plays important roles in various cell types. Additionally, intercellular flow transports cytoplasmic materials for rapid oocyte growth.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xiaolu Xu, Sabine Hummel, Klaus Harter, Uener Kolukisaoglu, Michael Riemann, Peter Nick
Summary: The transition to terrestrial plants led to a loss of microtubule minus-end-directed dynein motors and an expansion of class-XIV kinesins. OsDLK, a class-XIV kinesin from rice, plays a role in cell elongation during development and moves into the nucleus in response to cold stress. The import of OsDLK into the nucleus might be correlated with its specific DNA binding, particularly to the Opaque2 motif, which is found in the promoter region of NtAvr9/Cf9, a transcription factor involved in cold adaptation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Isabel Garcia-Saez, Dimitrios A. Skoufias
Summary: Eg5, a motor protein involved in mitotic spindle formation, has attracted interest as a potential target for cancer chemotherapy. While most inhibitors have shown limited efficacy, one inhibitor, Arry-520, has demonstrated clinical effectiveness in multiple myeloma patients and sparked new research trends including alternative binding sites and drug synergy. The field faces challenges in drug resistance development and improving therapeutic applications for Eg5 inhibitors.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pedro Monteiro, Bongwhan Yeon, Samuel S. Wallis, Susana A. Godinho
Summary: Intracellular organelle organization in eukaryotic cells is achieved by active transport along microtubules. Centrosome amplification, commonly observed in cancer, induces a change in organelle positioning that facilitates nuclear migration. Increased tubulin acetylation differentially regulates kinesin-1-mediated organelle displacement to regulate intracellular organization.
Article
Cell Biology
Shih-Chieh Ti, Melissa C. Pamula, Stuart C. Howes, Christian Duellberg, Nicholas I. Cade, Ralph E. Kleiner, Scott Forth, Thomas Surrey, Eva Nogales, Tarun M. Kapoor
DEVELOPMENTAL CELL
(2016)
Article
Cell Biology
Melissa C. Pamula, Shih-Chieh Ti, Tarun M. Kapoor
JOURNAL OF CELL BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Elizabeth H. Kellogg, Stuart Howes, Shih-Chieh Ti, Erney Ramirez-Aportela, Tarun M. Kapoor, Pablo Chacon, Eva Nogales
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biophysics
Chad D. McCormick, Matthew S. Akamatsu, Shih-Chieh Ti, Thomas D. Pollard
BIOPHYSICAL JOURNAL
(2013)
Correction
Biochemistry & Molecular Biology
Radhika Subramanian, Shih-Chieh Ti, Lei Tan, Seth A. Darst, Tarun M. Kapoor
Article
Cell Biology
Shih-Chieh Ti, Gregory M. Alushin, Tarun M. Kapoortv
DEVELOPMENTAL CELL
(2018)
Article
Biochemistry & Molecular Biology
Shih-Chieh Ti, Thomas D. Pollard
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Chemistry, Multidisciplinary
Ralph E. Kleiner, Shih-Chieh Ti, Tarun M. Kapoor
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2013)
Article
Multidisciplinary Sciences
Shih-Chieh Ti, Christopher T. Jurgenson, Bradley J. Nolen, Thomas D. Pollard
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Article
Biochemistry & Molecular Biology
Michal Wieczorek, Linas Urnavicius, Shih-Chieh Ti, Kelly R. Molloy, Brian T. Chait, Tarun M. Kapoor
Review
Cell Biology
Shih-Chieh Ti
Summary: This article provides an overview of the importance of tubulin in cellular structures and functions, and highlights the characteristics of purified α/β-tubulin heterodimers through in vitro experiments, revealing the regulatory mechanisms of microtubule dynamics and stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Tatsuya Fukuyama, Lucan Yan, Masahito Tanaka, Megumi Yamaoka, Kei Saito, Shih-Chieh Ti, Chung-Chi Liao, Kuo-Chiang Hsia, Yusuke T. Maeda, Yuta Shimamoto
Summary: The spindle is a dynamic intracellular structure essential for chromosome segregation during cell division. It can exhibit varied shape morphologies through nonrandom, bistable self-organization paths. The emergence of different spindle shapes is influenced by both physical and molecular factors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Chung-Hsu Cheng, Yu-Hui Lo, Shu-Shan Liang, Shih-Chieh Ti, Feng-Ming Lin, Chia-Hui Yeh, Han-Yi Huang, Ting-Fang Wang
GENES & DEVELOPMENT
(2006)
Article
Multidisciplinary Sciences
YK Chen, CH Leng, H Olivares, MH Lee, YC Chang, WM Kung, SC Ti, YH Lo, AHJ Wang, CS Chang, DK Bishop, YP Hsueh, TF Wang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2004)