Article
Biochemistry & Molecular Biology
Kim Kiat Lim, Hwei Yee Teo, Yuan Yee Tan, Yi Bing Zeng, Ulysses Tsz Fung Lam, Mahesh Choolani, Ee Sin Chen
Summary: MTHFR is a key enzyme in the folate metabolic pathway, linked to various human conditions. The fission yeast gene met11(+) shows similar function to MTHFR, affecting chromosome stability and meiotic fidelity. Cooperation among Met11, Rec8, and Sgo1 is crucial for proper genetic inheritance during mitosis and meiosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ingrid Billault-Chaumartin, Laetitia Michon, Caitlin A. Anderson, Sarah E. Yde, Cristian Suarez, Justyna Iwaszkiewicz, Vincent Zoete, David R. Kovar, Sophie G. Martin
Summary: In formin-family proteins, the nucleation and elongation activities of actin filaments are located in the formin homology 1 (FH1) and FH2 domains, with varying reaction rates among different formins. This study investigates the importance of nucleation and elongation rates of formins in vivo using the formin Fus1 in Schizosaccharomyces pombe. It is found that changes in the nucleation and elongation rates have direct effects on the architecture of the actin fusion focus, and that Fus1's native high nucleation and low elongation rates are optimal for assembly of the fusion focus. A point mutant in the FH2 domain of Fus1 is also identified, which preserves the native nucleation and elongation rates in vitro but alters the function in vivo, indicating additional properties of the FH2 domain. Thus, the rates of actin assembly are tailored for assembly of specific actin structures.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Sanju Ashraf, Ye Dee Tay, David A. Kelly, Kenneth E. Sawin
Summary: A novel microtubule-independent form of nuclear movement has been discovered in fission yeast, which relies on actin cables but not actin polymerization or myosin V forces. This movement is directed towards growing cell tips and is influenced by the proximity of the nucleus to the tip. Additionally, vesicle-associated membrane proteins are found to be critical for this microtubule-independent nuclear movement.
JOURNAL OF CELL SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Hokuto Ohtsuka, Yoko Otsubo, Takafumi Shimasaki, Akira Yamashita, Hirofumi Aiba
Summary: The ecl family genes in Schizosaccharomyces pombe are positive regulators of chronological lifespan and involved in various biological processes such as nutritional starvation and stress resistance.
MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kim Kiat Lim, Nathaniel Zhi Hao Koh, Yi Bing Zeng, Jun Kai Chuan, Raechell Raechell, Ee Sin Chen
Summary: Our study identified that disruption of membrane transport, chromosome segregation, and mitochondrial oxidative phosphorylation increase cellular susceptibility towards 5-FU, while null mutants of Ino80 complex factors exhibit resistance. Modulating the function of Ino80 complex factors may potentially reverse 5-FU resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Dan-Dan Xu, Li-Lin Du
Summary: Autophagy is a process that transports cellular components to the vacuole/lysosome, playing important roles in maintaining cellular homeostasis and stress resistance. In the fission yeast, autophagy is crucial for cell survival during nutrient depletion and ER stress. Research on fission yeast autophagy machinery has revealed similarities and differences with other eukaryotic models, such as budding yeast Saccharomyces cerevisiae. Recent discoveries of selective autophagy pathways in fission yeast provide new insights into cargo selectivity in autophagy.
Review
Cell Biology
Masamitsu Sato, Yasutaka Kakui, Mika Toya
Summary: Meiosis is a specialized cell division process for gamete production in eukaryotes, with chromosomes and microtubules mutually influencing each other. Studies on the fission yeast Schizosaccharomyces pombe have revealed the organization of chromosomes, cytoskeleton, and cell cycle progression in mitosis and meiosis. Meiosis is strategically designed for faithful genetic material segregation and genetic diversity production through meiosis-specific and general factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Francisco Prieto-Ruiz, Elisa Gomez-Gil, Rebeca Martin-Garcia, Armando Jesus Perez-Diaz, Jero Vicente-Soler, Alejandro Franco, Teresa Soto, Pilar Perez, Marisa Madrid, Jose Cansado
Summary: Under aerobic respiration conditions, the fission yeast Schizosaccharomyces pombe regulates the activity of myosin II through redundant phosphorylation of its homolog Rlc1 by the kinases Pak1 and Pak2, which is essential for cytokinesis and cell growth. Additionally, the stress-activated protein kinase pathway controls the integrity of myosin II. The fine-tuned interplay between myosin II function and actin filament availability, controlled by Rlc1 phosphorylation and environmental factors, is critical for successful cytokinesis during aerobic respiration.
Article
Biochemistry & Molecular Biology
Lenka Halova, David Cobley, Mirita Franz-Wachtel, Tingting Wang, Kaitlin R. Morrison, Karsten Krug, Nicolas Nalpas, Boris Macek, Iain M. Hagan, Sean J. Humphrey, Janni Petersen
Summary: Fluctuations in TOR, AMPK and MAP-kinase signaling play a crucial role in maintaining cellular homeostasis and coordinating growth and division with environmental context. By studying the phosphorylation levels at more than 1000 sites following nitrogen stress or TOR inhibition, new targets previously not linked to nutritional or TOR control were identified. Nutritional control of gene expression, significant modulation of actin cytoskeletal components, and prevention of sexual differentiation by reduced phosphorylation of MAPKK Byr1 were observed in response to environmental stress.
Review
Microbiology
Hokuto Ohtsuka, Kazuki Imada, Takafumi Shimasaki, Hirofumi Aiba
Summary: The fission yeast S. pombe adapts to the environment and survives in nutrient-starved conditions through sexual differentiation and extension of the chronological lifespan. Spore formation in S. pombe is highly resistant to stresses and spores can survive for a long period. This minireview focuses on the molecular mechanisms of spore formation induced by starvation and highlights the presence of an effective survival mechanism.
Article
Endocrinology & Metabolism
Steffen Loke, Anna Stoll, David Machalz, Francesco Botre, Gerhard Wolber, Matthias Bureik, Maria Kristina Parr
Summary: Cytochrome P450s (CYPs) are essential enzymes in the human body, with CYP21A2 playing a key role in steroid biosynthesis. Research indicates that the interaction between the 3-oxo group and arginine-234 of the enzyme is a strict requirement in CYP21A2 substrates.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Hokuto Ohtsuka, Takafumi Shimasaki, Hirofumi Aiba
Summary: Studies have shown that sulfur plays essential roles in biological molecules and different yeast models exhibit varying sulfur metabolism pathways. Sulfur starvation induces various cellular responses in fission yeast, contributing to a better understanding of stress and lifespan regulation caused by sulfur depletion.
FEMS YEAST RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hokuto Ohtsuka, Takafumi Shimasaki, Hirofumi Aiba
Summary: This mini-review summarizes over 70 genes involved in the chronological lifespan of Schizosaccharomyces pombe and describes the signal transduction pathways affecting lifespan extension. These pathways and genes play important roles in regulating the chronological lifespan extension in S. pombe.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomas Selicky, Matus Jurcik, Barbora Mikolaskova, Alexandra Pitelova, Nina Mayerova, Miroslava Kretova, Michaela Osadska, Jan Jurcik, Roman Holic, Lenka Kohutova, Jana Bellova, Zsigmond Benko, Juraj Gregan, Silvia Bagelova Polakova, Peter Barath, Lubos Cipak, Ingrid Cipakova
Summary: This study demonstrates the important role of the Gpl1-Gih35-Wdr83 complex in regulating pre-mRNA splicing in the fission yeast S. pombe, through knock-out strategy, affinity purification, and yeast-two-hybrid assay.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
R. Sahaya Glingston, Jyoti Yadav, Jitika Rajpoot, Neha Joshi, Shirisha Nagotu
Summary: Yeast has been proven to be a valuable model system for studying viral infection and expression of viral proteins, advancing our understanding of important viruses. Through high-throughput analysis, genetic manipulation, and its compact genome, yeast provides insights into various aspects related to virus replication.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Shih-Chieh Ti, Melissa C. Pamula, Stuart C. Howes, Christian Duellberg, Nicholas I. Cade, Ralph E. Kleiner, Scott Forth, Thomas Surrey, Eva Nogales, Tarun M. Kapoor
DEVELOPMENTAL CELL
(2016)
Article
Cell Biology
Melissa C. Pamula, Shih-Chieh Ti, Tarun M. Kapoor
JOURNAL OF CELL BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Elizabeth H. Kellogg, Stuart Howes, Shih-Chieh Ti, Erney Ramirez-Aportela, Tarun M. Kapoor, Pablo Chacon, Eva Nogales
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biophysics
Chad D. McCormick, Matthew S. Akamatsu, Shih-Chieh Ti, Thomas D. Pollard
BIOPHYSICAL JOURNAL
(2013)
Article
Biochemistry & Molecular Biology
Radhika Subramanian, Shih-Chieh Ti, Lei Tan, Seth A. Darst, Tarun M. Kapoor
Correction
Biochemistry & Molecular Biology
Radhika Subramanian, Shih-Chieh Ti, Lei Tan, Seth A. Darst, Tarun M. Kapoor
Article
Cell Biology
Shih-Chieh Ti, Gregory M. Alushin, Tarun M. Kapoortv
DEVELOPMENTAL CELL
(2018)
Article
Chemistry, Multidisciplinary
Ralph E. Kleiner, Shih-Chieh Ti, Tarun M. Kapoor
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2013)
Article
Multidisciplinary Sciences
Shih-Chieh Ti, Christopher T. Jurgenson, Bradley J. Nolen, Thomas D. Pollard
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Article
Biochemistry & Molecular Biology
Michal Wieczorek, Linas Urnavicius, Shih-Chieh Ti, Kelly R. Molloy, Brian T. Chait, Tarun M. Kapoor
Review
Cell Biology
Shih-Chieh Ti
Summary: This article provides an overview of the importance of tubulin in cellular structures and functions, and highlights the characteristics of purified α/β-tubulin heterodimers through in vitro experiments, revealing the regulatory mechanisms of microtubule dynamics and stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Tatsuya Fukuyama, Lucan Yan, Masahito Tanaka, Megumi Yamaoka, Kei Saito, Shih-Chieh Ti, Chung-Chi Liao, Kuo-Chiang Hsia, Yusuke T. Maeda, Yuta Shimamoto
Summary: The spindle is a dynamic intracellular structure essential for chromosome segregation during cell division. It can exhibit varied shape morphologies through nonrandom, bistable self-organization paths. The emergence of different spindle shapes is influenced by both physical and molecular factors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Chung-Hsu Cheng, Yu-Hui Lo, Shu-Shan Liang, Shih-Chieh Ti, Feng-Ming Lin, Chia-Hui Yeh, Han-Yi Huang, Ting-Fang Wang
GENES & DEVELOPMENT
(2006)
Article
Multidisciplinary Sciences
YK Chen, CH Leng, H Olivares, MH Lee, YC Chang, WM Kung, SC Ti, YH Lo, AHJ Wang, CS Chang, DK Bishop, YP Hsueh, TF Wang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2004)