Article
Biochemical Research Methods
Behnam B. K. Shariati, Seyyede Sarvenaz Khatami, Mohammad Ali Ansari, Fazel Jahangiri, Hamid Latifi, Valery V. Tuchin
Summary: This paper proposes a new strategy of optical clearing called time-dependent or temporal tissue optical clearing (TTOC) to enhance the optical imaging quality. By altering the pulse width, the absorption and scattering of light in tissue can be regulated in the TTOC technique. Experimental investigations and computational simulations were conducted to study the dependence of optical properties on pulse width in gelatin-based phantom and modeled human skin tissue. The results provide insights into the penetration depth and reflectance of light in tissue for different pulse widths.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Multidisciplinary Sciences
Ruby Boateng, Nathaniel Boechat, Philipp P. Henrich, Ewelina Bolcun-Filas
Summary: Female fertility and reproductive lifespan are determined by the quality and quantity of ovarian oocyte reserve, which is influenced by three waves of oocyte elimination. Immunofluorescence and 3D visualization offer a new approach to study oocyte development. Research demonstrates that this method can quantitatively measure oocyte attrition during ovarian development in mice.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biology
Yasunari Seita, Keren Cheng, John R. McCarrey, Nomesh Yadu, Ian H. Cheeseman, Alec Bagwell, Corinna N. Ross, Isamar Santana Toro, Li-hua Yen, Sean Vargas, Christopher S. Navara, Brian P. Hermann, Kotaro Sasaki
Summary: This study successfully established a method for culturing mid-gestation mouse iPSCs on a feeder layer, which can further differentiate into cells similar to primordial germ cells (PGCLCs). Furthermore, these cells can acquire an early developmental germ cell phenotype when co-cultured with mouse testicular somatic cells.
Article
Cell Biology
Mingxia Ge, Li Hu, Hongshun Ao, Meiting Zi, Qingpeng Kong, Yonghan He
Summary: Senescent cells (SCs) accumulate with age and contribute to age-related diseases. Clearing SCs can delay aging, treat age-related diseases, and extend healthspan. Targeting the proteins in the SC anti-apoptotic pathways with small molecules can selectively kill SCs, but current senolytics still have limitations in terms of safety, specificity, and broad-spectrum activity. New strategies such as PROTAC technology, CAR T cells, and ?-galactosidase-modified prodrugs have shown promising potential in clearing SCs.
MECHANISMS OF AGEING AND DEVELOPMENT
(2021)
Article
Chemistry, Analytical
Yi-Meng Duan, Han-Wen Tian, Hua-Bin Li, Kun-Peng Wang, Shaojin Chen, Dong Sheng Guo, Zhi-Qiang Hu
Summary: Spermine, an important biomarker for cancer, can be detected and depleted using the supramolecular reporter pair PPDD@SC4AD. The complex of SC4AD and PPDD can release fluorescence in tumor cells. Moreover, the amphiphilicity of SC4AD drives the self-assembly of PPDD@SC4AD, ensuring an efficient enhanced permeability and retention effect in vivo. This study may inspire the development of new integrated approaches for cancer diagnosis and treatment.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Veterinary Sciences
Xuan Xie, Roman Franek, Martin Psenicka, Fan Chen, Vojtech Kaspar
Summary: This study optimized the cultivation conditions for germ cells in common carp, and the results showed that using hESC media and RTG2 cell line as feeder cells promoted germ cell proliferation and survival. Additionally, opening the carp from the dorsal side minimized the risk of contamination. These findings are important for common carp breeding, genetic resource conservation, and development of specific techniques.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Developmental Biology
Chun-Wei Feng, Guillaume Burnet, Cassy M. Spiller, Fiona Ka Man Cheung, Kallayanee Chawengsaksophak, Peter Koopman, Josephine Bowles
Summary: This study demonstrates the crucial role of retinoic acid responsive elements (RAREs) in initiating Stra8 expression in the fetal mouse ovary, along with the enhancement of Stra8 expression by the transcription factor DMRT1 in the presence of retinoic acid. The specific anterior-to-posterior pattern of Stra8 initiation in germ cells is faithfully recapitulated by the 2.9 kb Stra8 promoter, highlighting the importance of RAREs in regulating germ cell meiosis.
Article
Cell Biology
S. Soroush Nasseri, Erika M. J. Siren, Jayachandran N. N. Kizhakkedathu, Karen Cheung
Summary: Three-dimensional cell cultures are increasingly used as in vitro models to mimic in vivo tissues for drug screening. However, the penetration of three-dimensional microscopy techniques is limited by the light scattering of tissues. Traditional tissue clearing protocols are not suitable for small spheroids and organoids. In this study, a novel tissue clearing solution called HyClear was developed for small spheroids and organoids. This protocol allows for one-step tissue clearing and is compatible with high-throughput screening studies.
Article
Cell Biology
Maeve Ballantyne, Lorna Taylor, Tuanjun Hu, Dominique Meunier, Sunil Nandi, Adrian Sherman, Brenda Flack, John M. Henshall, Rachel J. Hawken, Mike J. McGrew
Summary: The study found that avian PGCs are not sexually restricted for functional gamete formation, as they can form functional gametes in both female and male hosts, providing new insights for the cryopreservation of poultry and other bird species using diploid stage germ cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Kaiana Recchia, Amanda Soares Jorge, Lais Vicari de Figueiredo Pessoa, Ramon Cesar Botigelli, Vanessa Cristiane Zugaib, Aline Fernanda de Souza, Daniele dos Santos Martins, Carlos Eduardo Ambrosio, Fabiana Fernandes Bressan, Naira Caroline Godoy Pieri
Summary: Follicle stimulating hormone (FSH) plays important roles in reproductive development, with disorders associated with mutations in FSH receptor and signaling pathways. The exact influence of FSH on germ cells is still unclear, but it may play a crucial role in spermatogenesis and folliculogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Political Science
John Hogan, Michael Howlett, Mary Murphy
Summary: This article examines the impact of the COVID-19 pandemic on policy ideas and routines, discussing the possibilities of policy transformations and clarifying the types of policy punctuations and their effects on policy paths.
POLICY AND SOCIETY
(2022)
Article
Biology
Weiwei Xu, Yajuan Yang, Yongze Yu, Canxin Wen, Simin Zhao, Lili Cao, Shidou Zhao, Yingying Qin, Zi-Jiang Chen
Summary: The study reveals that FAAP100 is essential for resolving transcription-replication conflicts and protecting replication forks during the proliferation of mouse primordial germ cells. Its loss leads to DNA damage and insufficient reproductive reserve. This finding is important for understanding germ cell development and genetic stability maintenance.
Editorial Material
Optics
Chris Xu
Summary: The research group has developed a skull-clearing technique that enables stable, high resolution optical imaging of the mouse brain through a transparent window for several weeks.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Muge Molbay, Zeynep Ilgin Kolabas, Mihail Ivilinov Todorov, Tzu-Lun Ohn, Ali Erturk
Summary: This review discusses the importance of tissue clearing in histological analysis of biological tissues, presenting different clearing methods and solutions to potential issues. Additionally, it compiles common problems and proposes solutions based on survey responses from researchers who employ tissue clearing techniques.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Article
Management
Evangelos Benos, Wenqian Huang, Albert Menkveld, Michalis Vasios
Summary: Fragmenting clearing across multiple central counterparties (CCPs) is costly, as global dealers cannot net positions across CCPs. This leads to the need for collateralizing both short and offsetting long positions across different CCPs, causing persistent differences in prices (CCP basis). Empirical tests using unique CCP data for interest-rate derivatives (IRDs) provide broad support for this idea and estimate daily clearing friction costs of $80 million for sellers clearing in LCH, the largest European CCP for IRDs.
MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Summer B. Thyme, Lindsey M. Pieper, Eric H. Li, Shristi Pandey, Yiqun Wang, Nathan S. Morris, Carrie Sha, Joo Won Choi, Kristian J. Herrera, Edward R. Soucy, Steve Zimmerman, Owen Randlett, Joel Greenwood, Steven A. McCarroll, Alexander F. Schier
Article
Biochemistry & Molecular Biology
Owen Randlett, Martin Haesemeyer, Greg Forkin, Hannah Shoenhard, Alexander F. Schier, Florian Engert, Michael Granato
Article
Biology
Naoya Yamaguchi, Tugba Colak-Champollion, Holger Knaut
Article
Biochemistry & Molecular Biology
Tugba Colak-Champollion, Ling Lan, Alisha R. Jadhav, Naoya Yamaguchi, Gayatri Venkiteswaran, Heta Patel, Michael Cammer, Martin Meier-Schellersheim, Holger Knaut
Article
Neurosciences
Manxiu Ma, Alexandro D. Ramirez, Tong Wang, Rachel L. Roberts, Katherine E. Harmon, David Schoppik, Avirale Sharma, Christopher Kuang, Stephanie L. Goei, James A. Gagnon, Steve Zimmerman, Shengdar Q. Tsai, Deepak Reyon, J. Keith Joung, Emre R. F. Aksay, Alexander F. Schier, Y. Albert Pan
JOURNAL OF NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Qian Lin, Jason Manley, Magdalena Helmreich, Friederike Schlumm, Jennifer M. Li, Drew N. Robson, Florian Engert, Alexander Schier, Tobias Nobauer, Alipasha Vaziri
Article
Cell Biology
Stephanie Lau, Anna Feitzinger, Gayatri Venkiteswaran, John Wang, Stephen W. Lewellis, Chad A. Koplinski, Francis C. Peterson, Brian F. Volkman, Martin Meier-Schellersheim, Holger Knaut
NATURE CELL BIOLOGY
(2020)
Editorial Material
Biochemical Research Methods
Alexander F. Schier
Article
Multidisciplinary Sciences
Tony Y-C Tsai, Mateusz Sikora, Peng Xia, Tugba Colak-Champollion, Holger Knaut, Carl-Philipp Heisenberg, Sean G. Megason
Article
Developmental Biology
Daniel S. Levic, Naoya Yamaguchi, Siyao Wang, Holger Knaut, Michel Bagnat
Summary: A simple approach for tagging proteins in zebrafish on their N or C termini with fluorescent proteins by inserting PCR-generated donor amplicons into non-coding regions of the corresponding genes has been developed, enabling the generation of endogenously tagged alleles for crucial genes in epithelial biology and organ development. This approach facilitates the generation of knock-in lines in zebrafish, paving the way for accurate quantitative imaging studies.
Article
Biology
Nathan D. Lord, Adam N. Carte, Philip B. Abitua, Alexander F. Schier
Summary: Embryos communicate instructions to cells through morphogens, which form gradients by diffusing from a source. However, natural patterning systems involve co-factors and signaling feedback, suggesting additional mechanisms are needed for signaling patterns. For the mesendoderm inducer Nodal in zebrafish embryos, the range of Nodal ligands is regulated by the EGF-CFC co-receptor Oep to control the spread and sensitivity of signaling, as confirmed by computational modeling and experimental observations.
Article
Cell Biology
Naoya Yamaguchi, Ziyi Zhang, Teseo Schneider, Biran Wang, Daniele Panozzo, Holger Knaut
Summary: This study reports a new approach to measure cell migration stresses in zebrafish embryos and suggests that collectively migrating cells may be pushed forward by their rear cells. The findings have important implications for understanding animal embryonic development and tissue motion.
NATURE CELL BIOLOGY
(2022)
Article
Ecology
Maxwell E. R. Shafer, Ahilya N. Sawh, Alexander F. Schier
Summary: Single-cell RNA sequencing of hypothalamic cells from zebrafish and Mexican tetra revealed over 75% shared cell types between the two species. Shared cell types displayed shifts in paralogue expression, while species-specific cell types were enriched for the expression of species-specific genes.
NATURE ECOLOGY & EVOLUTION
(2022)
Review
Developmental Biology
Naoya Yamaguchi, Holger Knaut
Summary: Cell-extracellular matrix interactions are essential for cell anchoring and migration, with focal adhesions (FAs) playing a key role. However, the role of FAs in vivo is still not well understood.
Article
Cell Biology
Brian A. Kinney, Arwa Al Anber, Richard H. Row, Yu-Jung Tseng, Maxwell D. Weidmann, Holger Knaut, Benjamin L. Martin
