Article
Chemistry, Multidisciplinary
Norimichi Nagano, Yuki Ichihashi, Toru Komatsu, Hiroyuki Matsuzaki, Keisuke Hata, Toshiaki Watanabe, Yoshihiro Misawa, Misa Suzuki, Shingo Sakamoto, Yu Kagami, Ayumi Kashiro, Keiko Takeuchi, Yukihide Kanemitsu, Hiroki Ochiai, Rikiya Watanabe, Kazufumi Honda, Yasuteru Urano
Summary: In this study, a fluorogenic substrate probe was developed to analyze enzymatic activity changes in tissue and plasma samples. The probe was particularly useful for studying enzyme activities in a single-molecule enzyme assay platform. We detected the activity of neurolysin in plasma samples and found higher enzyme activity in the blood samples of colorectal tumor patients. The results suggest that single-molecule neurolysin activity could be a promising blood biomarker for colorectal cancer diagnosis.
Article
Biochemistry & Molecular Biology
T. Etelainen, V Kulmala, R. Svarcbahs, M. Jantti, T. T. Myohanen
Summary: Oxidative stress is a common toxic feature in various neurodegenerative diseases, and reducing ROS production by inhibiting PREP can provide neuroprotection.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Julio C. Padovan, Thales M. H. Dourado, Gustavo F. Pimenta, Thiago Bruder-Nascimento, Carlos R. Tirapelli
Summary: High consumption of ethanol increases the risk of cardiovascular diseases, particularly arterial hypertension. Ethanol-induced hypertension is a complex event involving oxidative stress as a hallmark of vascular dysfunction. Reactive oxygen species (ROS) play a crucial role in triggering various pathological processes, including intracellular calcium accumulation, reduced bioavailability of nitric oxide, activation of mitogen-activated protein kinases, endothelial dysfunction, and loss of the anticontractile effect of perivascular adipose tissue.
Article
Multidisciplinary Sciences
Yao Li, Damir Kracun, Christopher M. Dustin, Mohamed El Massry, Shuai Yuan, Christian J. Goossen, Evan R. DeVallance, Sanghamitra Sahoo, Cynthia St Hilaire, Aditi U. Gurkar, Toren Finkel, Adam C. Straub, Eugenia Cifuentes-Pagano, Patrick J. Pagano
Summary: In an investigation into signaling triggered by aging and hyperglycemia, researchers found that NADPH Oxidase (NOX) plays a crucial role in driving cell damage, inflammation, and cellular senescence. Inhibiting NOX1 can reverse age-related impairments in blood flow and angiogenesis, as well as disrupt proinflammatory signaling associated with senescence. Targeting the NOX1-SASP signaling axis is predicted to be an effective strategy for mitigating vascular and organ system senescence.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Leticia Guadalupe Fragoso-Morales, Jose Correa-Basurto, Martha Cecilia Rosales-Hernandez
Summary: Alzheimer's disease is a major human dementia characterized by memory loss associated with oxidative stress in brain cells. Overexpression of NADPH oxidase in AD leads to the production of large amounts of reactive oxygen species, damaging brain cells and vasculature, making it a potential therapeutic target for AD.
Article
Biochemistry & Molecular Biology
Raquel Leao Neves, Alyne Marem, Bruno Carmona, Julia Galanakis Arata, Marcos Paulo Cyrillo Ramos, Giselle Zenker Justo, Fabiana Henriques Machado de Melo, Vitor Oliveira, Marcelo Yudi Icimoto
Summary: The study found that oxidative stress influences the expression and regulation of THOP in leukemia cells, which may affect the immune evasion of tumor cells. Additionally, the proteolytic activity of THOP is also associated with the cellular redox state.
Article
Biochemistry & Molecular Biology
Jiuchun Qiu, Daiqi Liu, Pengsha Li, Lingling Zhou, Lu Zhou, Xing Liu, Yue Zhang, Meng Yuan, Gary Tse, Guangping Li, Tong Liu
Summary: Oxidative stress and mitochondrial dysfunction play important roles in the development of diabetic cardiomyopathy in type 2 diabetes mellitus. This study established a type 2 diabetes model and found that inhibiting NADPH oxidase can improve ventricular remodeling and reduce the incidence of DCM.
Article
Cell Biology
Mei Xu, Yiding Zhao, Wenhao Zhang, Mengyang Geng, Qian Liu, Qian Gao, Ling Shuai
Summary: In this study, a unique rat haploid system was used to investigate the key modulators on pluripotency exiting in rat embryonic stem cells (ESCs). The findings provide an ideal tool for studying functional genomics in rats.
CELL PROLIFERATION
(2022)
Review
Cell Biology
Kazumi Takaishi, Hiroyuki Kinoshita, Shingo Kawashima, Shinji Kawahito
Summary: This article discusses the critical role of superoxide in vascular redox signaling and the physiological and pathophysiological functions of NOX in vascular smooth muscle cells. NOX is associated with cardiovascular diseases such as hypertension and diabetes, and is regulated by various stimulants.
Article
Cell Biology
Biljana Musicki, Serkan Karakus, Justin D. La Favor, Haolin Chen, Fabio H. Silva, Mikael Sturny, Barry R. Zirkin, Arthur L. Burnett
Summary: The study suggests that stimulating the production of endogenous testosterone can help reduce priapism in patients with SCD without affecting fertility. This treatment approach may work by activating TSPO, restoring phosphodiesterase 5 activity, and reducing oxidative stress levels.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Keum-Jin Yang, Won Jung Choi, Yoon-Kyung Chang, Cheol Whee Park, Suk Young Kim, Yu Ah Hong
Summary: Xanthine oxidase (XO) inhibition showed renoprotective effects in diabetic kidney disease (DKD) by inhibiting vascular endothelial growth factor (VEGF) and NADPH oxidase (NOX) and reducing oxidative stress. This study provides important insights into the therapeutic potential of XO inhibition in DKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Yuri Zilberter, Dennis R. Tabuena, Misha Zilberter
Summary: Neurodegenerative diseases (NDDs) causing cognitive impairment and dementia are difficult to treat due to a lack of understanding of their primary initiating factors. Recent research suggests that early oxidative stress caused by NADPH oxidase (NOX) activation may be a shared initiating mechanism among major sporadic NDDs. This study focuses on Alzheimer's disease (AD), Parkinson's disease (PD), and acquired epilepsy, and explores the commonalities in neuroinflammation and NOX involvement to identify therapeutic targets and gain a deeper understanding of the underlying causes of NDDs.
PROGRESS IN NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eloisa Aparecida Vilas-Boas, Lisa Nalbach, Emmanuel Ampofo, Camila Ferraz Lucena, Lea Naudet, Fernanda Ortis, Angelo Rafael Carpinelli, Bruce Morgan, Leticia Prates Roma
Summary: Modern lifestyles, lack of physical activity, poor nutrition, and high levels of saturated free fatty acids in obese individuals contribute to the increasing prevalence of obesity and type 2 diabetes. The study suggests that transient NOX2-dependent H2O2 production may be a key factor in early palmitate-induced lipotoxic effects.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Peter Benko, Katalin Gemes, Attila Feher
Summary: The metabolism and regulation of cellular polyamines play a crucial role in maintaining cell homeostasis and function. Polyamine oxidases (PAOs) generate hydrogen peroxide (H2O2) during catabolic and back-conversion processes, which has been found to be involved in various plant developmental processes and stress responses. The relationship between PAOs and NADPH oxidases (RBOHs) in controlling cellular reactive oxygen species is discussed, suggesting that these enzymes regulate each other's abundance/function indirectly through H2O2.
Review
Cell Biology
Timothy Fabisiak, Manisha Patel
Summary: Neuroinflammation and oxidative stress play important roles in the pathophysiology of epilepsy. They have significant crosstalk and influence each other through various mechanisms, affecting epileptic processes and disease outcomes. Treatment strategies targeting oxidative stress and redox regulation may help control neuroinflammation and improve patient outcomes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Emma Harper, Keith D. Rochfort, Diarmuid Smith, Philip M. Cummins
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2020)
Article
Critical Care Medicine
Robert G. Wallace, Mary-Rose Kenealy, Aidan J. Brady, Laura Twomey, Emer Duffy, Bernard Degryse, David Caballero-Lima, Niall M. Moyna, Marc-Antoine Custaud, Gerardene Meade-Murphy, Aoife Morrin, Ronan P. Murphy
Article
Physiology
Adrien Robin, Aline Auvinet, Bernard Degryse, Ronan Murphy, Marie-Pierre Bareille, Arnaud Beck, Claude Gharib, Guillemette Gauquelin-Koch, Aude Daviet, Francoise Larcher, Marc-Antoine Custaud, Nastassia Navasiolava
FRONTIERS IN PHYSIOLOGY
(2020)
Review
Pharmacology & Pharmacy
Robert G. Wallace, Keith D. Rochfort, Peter Barabas, Timothy M. Curtis, Hironori Uehara, Balamurali K. Ambati, Philip M. Cummins
Summary: The Angiopoietin-1/2 system shows potential for therapeutic intervention in a wide range of vascular pathologies, particularly through its association with endothelium. Engineered COMP-Ang1 has demonstrated greater potency in activating the Tie2 pathway compared to native Ang1, with multiple benefits reported including promotion of skeletal muscle regeneration, wound healing and angiogenesis.
VASCULAR PHARMACOLOGY
(2021)
Review
Physiology
Kristina R. Gopcevic, Eugenia Gkaliagkousi, Janos Nemcsik, Omur Acet, M. Rosa Bernal-Lopez, Rosa M. Bruno, Rachel E. Climie, Nikolaos Fountoulakis, Emil Fraenkel, Antonios Lazaridis, Petras Navickas, Keith D. Rochfort, Agne Satrauskiene, Jurate Zupkauskiene, Dimitrios Terentes-Printzios
Summary: This paper discusses the phenomenon of vascular aging resulting from various factors, and the important molecular markers associated with vascular aging. Further criteria need to be met for these molecules to be classified as biomarkers of vascular aging.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
H. Forde, C. Davenport, K. D. Rochfort, R. G. Wallace, E. Durkan, A. Agha, C. J. Thompson, W. T. Tormey, D. J. O'Gorman, P. M. Cummins, D. Smith
Summary: This study compares serum levels of TRAIL, OPG, and OPG/TRAIL ratio in individuals with type 2 diabetes mellitus (T2DM), with and without a history of cardiovascular disease (CVD), and controls, and suggests that the OPG/TRAIL ratio may serve as a biomarker for cardiovascular risk in T2DM patients.
DIABETES RESEARCH AND CLINICAL PRACTICE
(2022)
Review
Chemistry, Physical
Brian Freeland, Eanna McCarthy, Rengesh Balakrishnan, Samantha Fahy, Adam Boland, Keith D. Rochfort, Michal Dabros, Roger Marti, Susan M. Kelleher, Jennifer Gaughran
Summary: The EU emits 13.4 Mt of CO2 annually from plastic production, with the majority of plastics being produced from fossil fuel sources. In Europe, 29 Mt of plastic waste was collected in 2019, with only 32% being recycled. The life-sciences sector alone generates approximately 5.5 Mt/yr of plastic waste, most of which is disposed of by incineration. There is a growing interest in using bioplastics as a viable alternative for single-use lab consumables, but limited examples currently exist.
Review
Nutrition & Dietetics
Helena Mylise Sorensen, Keith D. Rochfort, Susan Maye, George MacLeod, Dermot Brabazon, Christine Loscher, Brian Freeland
Summary: Lactic acid bacteria (LAB) produce exopolysaccharides (EPS) during fermentation, which play an important role in fermented dairy products and have beneficial effects on human health. Understanding the parameters and conditions for EPS production from LAB is crucial for its application in the food industry.
Article
Biochemistry & Molecular Biology
N. Arul Manikandan, Ronan McCann, Dimitrios Kakavas, Keith D. Rochfort, Sithara P. Sreenilayam, Godze Alkan, Tom Stornetta, Allan Robert McGivern, Konstantinos Grintzalis, Bernd Friedrich, Greg Foley, Dermot Brabazon, Brian Freeland
Summary: Food spoilage leads to increased carbon dioxide emissions and food processing demand. This study developed anti-bacterial coatings using inkjet printing of silver nano-inks on food-grade polymer packaging, aiming to enhance food safety and reduce spoilage. Silver nanoparticles were synthesized and characterized, and all silver nanoparticles showed strong antibacterial activity against E. coli. The printed silver nano-inks on polymer reduced the bacterial cell population, and the bactericidal performance was comparable to penicillin-coated polymer. Ecotoxicity testing on daphniids showed the impact on freshwater environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Yongda Li, Keith D. Rochfort, David Collins, Konstantinos Grintzalis
Summary: The study presents a comparison of optimized protocols for the quantification of DNA using common intercalator dyes, allowing for fast and accurate detection. The methods were successfully applied to DNA detection in soil samples.
Meeting Abstract
Physiology
D. S. Kostyunina, E. Dillon, K. D. Rochfort, P. M. Cummins, P. McLoughlin
Meeting Abstract
Critical Care Medicine
D. Kostyunina, E. Dillon, K. D. Rochfort, P. M. Cummins, P. McLoughlin
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Meeting Abstract
Medicine, General & Internal
Daria Kostyunina, Eugene Dillon, Keith Rochfort, Philip Cummins, Paul McLoughlin
IRISH JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Physiology
Hannah Forde, Emma Harper, Keith D. Rochfort, Robert G. Wallace, Colin Davenport, Diarmuid Smith, Philip M. Cummins
PHYSIOLOGICAL REPORTS
(2020)
Meeting Abstract
Critical Care Medicine
D. Kostyunina, K. D. Rochfort, P. M. Cummins, P. McLoughlin
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2020)