Article
Biochemistry & Molecular Biology
Julia Bauer, Nicole Cuvelier, Nada Ragab, Katja Simon-Keller, Frauke Nitzki, Natalie Geyer, Dominik S. Botermann, Dominik P. Elmer, Albert Rosenberger, Thomas A. Rando, Stefano Biressi, James A. Fagin, Dieter Saur, Christian Dullin, Hans-Ulrich Schildhaus, Walter Schulz-Schaeffer, Fritz Aberger, Anja Uhmann, Heidi Hahn
Summary: It has been found that in ERMS, the Hedgehog (HH)/GLI signaling pathway and RAS signaling pathway interact with each other, with RAS mutations able to increase proliferation and tumorigenicity by inhibiting the HH signaling pathway. In a mouse model, different RAS mutations have different effects on ERMS development.
Article
Multidisciplinary Sciences
Fernando C. Baltanas, Rosula Garcia-Navas, Pablo Rodriguez-Ramos, Nuria Calzada, Cristina Cuesta, Javier Borrajo, Rocio Fuentes-Mateos, Andrea Olarte-San Juan, Nerea Vidana, Esther Castellano, Eugenio Santos
Summary: The impact of genetic ablation of SOS1 or SOS2 was evaluated in a murine model of KRAS(G12D)-driven lung adenocarcinoma (LUAD). The study found that SOS1 plays a critical role in the development and survival of KRAS(G12D)-driven LUAD, and its ablation can significantly increase the lifespan of mice and reduce tumor burden and populations of cancer-associated cells in the tumor microenvironment.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Shigeo Otake, Yuka Itoh, Chiho Omata, Masao Saitoh, Keiji Miyazawa
Summary: E-cadherin can be downregulated in KRAS-mutated cancer cells through a novel regulatory switch involving ZEB1 and oncogenic K-Ras, which requires an external cue for temporal downregulation. This regulatory mechanism is distinct from EPCAM repression by ZEB1 and contributes to phenotypic plasticity during malignant progression.
Article
Chemistry, Analytical
Neng Ling, Huiming Liu, Junxiao Guo, Zhouliang Liang, Yibin Zhang, Hui Li, Hui Wu, Tiantian Xie, Yijun Yuan, Xiahui Li, Menglan Peng, Xianhua Wei, Long Liang, Jing Liu, Wencan Wu, Mao Ye
Summary: In this study, a DNA aptamer expression system was developed to generate DNA aptamers with functional activity in mammalian cells by mimicking retroviruses. DNA aptamers targeting intracellular Ras and membrane-bound CD71 were successfully generated in cells. The expressed Ras aptamer not only specifically bound to the intracellular Ras protein but also inhibited the phosphorylation of downstream ERK1/2 and AKT. Furthermore, by inserting the DNA aptamer expression system into a lentivirus vector, stable production of the aptamer in cells resulted in the inhibition of lung cancer cell proliferation.
ANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Dachuan Gu, Shuhua Wu, Zhenming Yu, Lanting Zeng, Jiajia Qian, Xiaochen Zhou, Ziyin Yang
Summary: This study investigates the effects of histone acetylations on the accumulation of herbivore-induced plant volatiles (HIPVs) in tea plants, specifically focusing on the regulation of CsHDA2-CsMYC2 transcriptional module. The results provide insights into the characterization of plant aromatic compounds and the understanding of epigenetic regulation in response to external stress.
HORTICULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Byeong Hyeok Choi, Ziyue Kou, Tania Marlyn Colon, Chih-Hong Chen, Yuan Chen, Wei Dai
Summary: Radil, a downstream effector of Ras, interacts strongly with KRas and GTP-bound Ras, suggesting a role in Ras activation. Overexpression of Radil leads to transient activation of MAPK signaling, while Radil knockdown weakens downstream Ras signaling, affecting cell proliferation, invasion, and migration. Radil plays a vital role in regulating Ras signaling, cell adhesion, and the epithelial-mesenchymal transition, potentially offering new opportunities for Ras-related anticancer drug development.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Cheng-Yao Chiang, Songqing Fan, Hongmei Zheng, Wenjun Guo, Zehan Zheng, Yihua Sun, Chuanqi Zhong, Juan Zeng, Shuaihu Li, Min Zhang, Tian Xiao, Duo Zheng
Summary: This study reveals the tumor-suppressive role of SETD7 in non-small cell lung cancer (NSCLC) by modulating KRAS methylation and degradation. SETD7 interacts with KRAS and methylates KRAS at lysines 182 and 184, leading to KRAS degradation and attenuation of the RAS/MEK/ERK signaling cascade.
Article
Environmental Sciences
Junjie Hu, Yan Yang, Xiaomei Lv, Zhilang Lao, Lili Yu
Summary: The study found that DDT metabolites DDA and DDMU induce DNA hypomethylation by inhibiting DNMT1 activity, leading to changes in the expression of sexual development-related genes.
ENVIRONMENTAL POLLUTION
(2021)
Article
Medicine, Research & Experimental
Junzhong Lai, Jiadi Liang, Yong Zhang, Bingchen Zhang, Jianhui Wei, Jiqiang Fan, Linqin Chen, Zhirong Chen, Qiumei Li, Dong Guo, Jizhen Lin, Qi Chen
Summary: DNA methyltransferase inhibitor Zebularine has shown advantages and enhanced therapeutic efficacy in tumour immunotherapy, but higher dosage is needed due to lack of targeted functionality. This study developed a nanodrug formulation called CS-Zeb nanoparticles, which exhibited enhanced drug release in an acidic environment. In vivo studies showed that CS-Zeb nanoparticles significantly reduced tumour burden, prolonged survival duration, and increased CD8+ T cells. The dosage of CS-Zeb nanoparticles was reduced by 70-fold compared to standalone Zebularine.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Pathology
Amy K. Kim, Selena Y. Lin, Zhili Wang, Harry Luu, James P. Hamilton, Wei Song, Ying-Hsiu Su
Summary: This study evaluated the impact of cell debris and 7-day room temperature storage on the quality and yield of transrenal DNA. The results showed that cell debris can be associated with a certain percentage of transrenal ucfDNA, while there was no significant difference in DNA degradation between room temperature and frozen samples. These findings are important for understanding the pre-analytical handling of transrenal circulating tumor DNA for HCC screening.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2023)
Article
Cell Biology
Chuan Zhang, Lu Wang, Chi Jin, Jiahui Zhou, Chaofan Peng, Yong Wang, Ziwei Xu, Dongsheng Zhang, Yuanjian Huang, Yue Zhang, Dongjian Ji, Wen Peng, Kangpeng Jin, Junwei Tang, Yifei Feng, Yueming Sun
Summary: Colorectal cancer is a common cancer worldwide, with liver metastasis being a key factor affecting prognosis. A study revealed that Lnc-LALC can regulate CRC cell metastasis ability by affecting DNA methylation status, highlighting its important role in CRLM.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Daisuke Aibara, Shogo Takahashi, Tomoki Yagai, Donghwan Kim, Chad N. Brocker, Moshe Levi, Kimihiko Matsusue, Frank J. Gonzalez
Summary: This study reveals that PPARA promotes hepatocyte proliferation through the activation of the E2F8-UHRF1-CDH1 axis, which involves epigenetic regulation. These findings provide important insights into the regulation of hepatocyte proliferation.
Article
Biology
Ian M. Ahearn, Helen R. Court, Farid Siddiqui, Daniel Abankwa, Mark R. Philips
Summary: ICMT is crucial for the efficient delivery of NRAS to the plasma membrane, as well as for its carboxyl methylation, binding with PDE6d, and flux through the Golgi.
LIFE SCIENCE ALLIANCE
(2021)
Article
Hematology
Zhi Wen, Grant Yun, Alexander Hebert, Guangyao Kong, Erik A. Ranheim, Remington Finn, Adhithi Rajagoplan, Shuyi Li, Yun Zhou, Mei Yu, Alisa Damnernsawad, Jeroen P. Roose, Joshua J. Coon, Renren Wen, Demin Wang, Jing Zhang
Summary: ETP-ALL is characterized by hyperactivation of cytokine receptor/Ras signaling, with downregulation of WT KRAS and Rasgrp1 serving as negative regulators of Ras/ERK pathway, contributing to cell proliferation in oncogenic Nras-driven ETP-like leukemia.
Article
Dentistry, Oral Surgery & Medicine
Shaoru Wang, Wei Wang, Shengchao Zhang, Fang Yang, Jing Qiu, Qingyuan Guo, Jiawei Zheng, Zhenggang Chen
Summary: The study showed that ICMT plays a crucial role in the progression of TSCC by promoting cell proliferation and tumor growth. Knockdown of ICMT induces apoptosis and cell cycle arrest, and weakens cell migration and invasion in TSCC cells. Mechanistically, ICMT deficiency disrupts the membrane targeting of K-Ras and RhoA, leading to the suppression of their downstream signaling.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yuseung Yi, Seong-Joo Heo, Jai-Young Koak, Seong-Kyun Kim
Summary: This retrospective clinical study aimed to evaluate the incidence and consequences of mechanical complications in components of internal conical connection implants and analyze the risk indicators. The results showed that molar implant-supported restorations and male patients had a higher risk of mechanical complications. Gold screws had a lower risk of screw loosening but a higher risk of fracture compared to titanium screws. Narrow-diameter implants had a higher risk of fracture.
JOURNAL OF PROSTHETIC DENTISTRY
(2023)
Article
Engineering, Electrical & Electronic
Oh-Kee Kwon, Chul-Wook Lee, Ki-Soo Kim
Summary: We present an electroabsorption modulator (EAM)-integrated distributed Bragg reflector laser diode (DBR-LD) with high data rate capabilities and wide wavelength tuning. The chip features low threshold current, large tuning range, and high static extinction ratio. Clear eye openings were observed at different data rates, demonstrating excellent modulation performance.
Article
Dentistry, Oral Surgery & Medicine
Yuseung Yi, Seong-Joo Heo, Jai-Young Koak, Seong-Kyun Kim
Summary: The study retrospectively analyzed the success of implant-assisted removable partial dentures (IARPDs) in three strategic positions in distal extension areas of Kennedy Class I and II arches. The results showed that the treatment type did not significantly affect the success rate of IARPDs, but the strategic position of the implants was associated with success rate.
JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY
(2023)
Article
Chemistry, Medicinal
Hye Ryeong Kim, Jonghwan Kim, Jae Sik Yu, Bum Soo Lee, Ki Hyun Kim, Chung Sub Kim
Summary: Dermabacter vaginalis, isolated from vaginal fluid of a Korean female in 2016, yielded a rare imidazolium metabolite, Dermazolium A. The chemical structures of Dermazolium A and five known metabolites were determined by NMR, HRMS, and MS/MS data analysis. Antibacterial tests revealed that Dermazolium A exhibited mild antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) USA300, Lacticaseibacillus paracasei subsp. paracasei KCTC 3510, and Brevibacterium epidermidis KCTC 3090 with MIC values of 41 μg/mL.
ARCHIVES OF PHARMACAL RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Eun Jin Park, Ba Wool Lee, Byeol Ryu, Hyo Moon Cho, Seong Kyun Kim, Seong-Sik Yoo, Eunhee Kim, Won Keun Oh
Summary: This study aimed to discover an anti-influenza herbal mixture and evaluate its antiviral activity against avian influenza. Three different medicinal plants with distinct medicinal purposes were selected and formulated into a combination, which showed potent inhibitory effects on the virus and reduced mortality and virus titers in chickens infected with avian influenza.
Article
Health Care Sciences & Services
Sung Shin Kim, Jun Sik Park, Dongil Choi, Yumi Shin, Kyeyeob Jo, Youngkyung Kim, Sun Ok Kim, Hyosun Kweon, Anna Lee, Young-Hyeon Bae
Summary: This study aimed to identify the optimal posture for the prevention of pressure ulcers by analyzing pressure changes applied to the pelvic region. It was found that lateral rotation combined with changes in the angles of the trunk and knees effectively prevented pressure ulcers.
TECHNOLOGY AND HEALTH CARE
(2023)
Article
Biophysics
Hyun Kyong Shon, Jin Gyeong Son, Sun Young Lee, Jeong Hee Moon, Ga Seul Lee, Kyoung-Shim Kim, Tae Geol Lee
Summary: In the study of degenerative brain diseases, changes in lipids, which are the main component of neurons, are crucial for indicating pathological changes. The researchers used ToF-SIMS with pulsed argon cluster ions to measure biomolecules, including lipids, in normal mouse brain tissue. They used OrbiSIMS, a hybrid SIMS system with an orbitrap mass analyzer, to identify the biomolecules in real tissue samples. The identification results by OrbiSIMS were confirmed by LC-MS/MS analysis of lipids extracted from mirror image tissue samples.
Article
Plant Sciences
Chen Huo, Sullim Lee, Min Jeong Yoo, Bum Soo Lee, Yoon Seo Jang, Ho Kyong Kim, Seulah Lee, Han Yong Bae, Ki Hyun Kim
Summary: Kaempferia parviflora, also known as Thai ginseng or black ginger, is a tropical medicinal plant traditionally used for treating various ailments. In this study, we isolated six methoxyflavones from the rhizomes of K. parviflora and found that two of them showed significant anti-melanogenic activity.
Article
Engineering, Biomedical
Joo-Eun Lee, Se Yun Jeong, Zijun Li, Hyun-Yi Kim, Hyun-Woo Kim, Min Jeong Yoo, Hee Joo Jang, Do-Kyun Kim, Namki Cho, Hee Min Yoo, Ki Hyun Kim
Summary: This study reports a screening platform to test the antiviral activity of natural products against SARS-CoV-2 and confirms their efficacy using lung organoids. Flavonoids from the natural product library showed strong antiviral activity against SARS-CoV-2 and did not affect cell viability. The screening platform provides a promising system for discovering novel drugs and potential therapeutics for COVID-19.
BIOMATERIALS RESEARCH
(2023)
Article
Chemistry, Medicinal
Da Eun Lee, Kun Hee Park, Joo-Hyun Hong, Seon Hee Kim, Ki-Moon Park, Ki Hyun Kim
Summary: The active constituent ursolic aldehyde isolated from the fruit extract of H. rhamnoides showed significant osteogenic activity and upregulated the expression of bone formation-related biomarkers. These findings suggest the potential of H. rhamnoides fruit extract as a nutraceutical for promoting bone health.
ARCHIVES OF PHARMACAL RESEARCH
(2023)
Article
Nutrition & Dietetics
Dahae Lee, Seulah Lee, Yoon Seo Jang, Rhim Ryoo, Jung Kyu Kim, Ki Sung Kang, Ki Hyun Kim
Summary: This study investigated the cytotoxicity of the mushroom Calvatia nipponica in human breast cancer cells and identified potential anti-tumor compounds. The results suggest that N,N-dimethyl-anthranilic acid may have anti-breast cancer activity and induce apoptosis through extrinsic and intrinsic signaling pathways.
Article
Biochemistry & Molecular Biology
Sullim Lee, Yea Jung Choi, Chen Huo, Akida Alishir, Ki Sung Kang, Il-Ho Park, Taesu Jang, Ki Hyun Kim
Summary: Human skin is composed of the epidermis and dermis, which work together to maintain the skin's tensile strength. Skin aging, however, leads to the degradation of collagen and elastin fibers, resulting in wrinkles, pigmentation, and sagging. This study investigated natural products derived from ginkgo fruit that have protective effects against TNF-alpha-induced skin aging.
Review
Plant Sciences
Grant Koher, Ajmal Khan, Gabriel Suarez-Vega, Pornphimon Meesakul, Ann-Janin Bacani, Tomomi Kohno, Xuewei Zhu, Ki Hyun Kim, Shugeng Cao, Zhenquan Jia
Summary: The plants P. albidus, P. forbesii, P. kauaiensis, and P. ruber, collectively known as mamaki, have cultural and traditional significance in Native Hawaiian medicine. Mamaki teas, particularly bottled Shaka tea, have gained popularity in Hawaii and the United States. This review consolidates current knowledge on mamaki, highlighting its potential therapeutic properties, physical characteristics, nutritional composition, and active phytochemicals, as well as recent advances in its antibacterial, antiviral, chemopreventive, anti-inflammatory, and antioxidant activities. Future prospects in this field are also discussed.
Article
Chemistry, Multidisciplinary
Arjun Prasad Timilsina, Bimal Kumar Raut, Chen Huo, Karan Khadayat, Prakriti Budhathoki, Mandira Ghimire, Rabin Budhathoki, Niraj Aryal, Ki Hyun Kim, Niranjan Parajuli
Summary: Metabolomics and molecular networking approaches have been rapidly advancing in the field of biological sciences, allowing for the systematic identification and characterization of bioactive metabolites in natural products. This study focused on evaluating the potential of four medicinal plants in inhibiting enzymatic activity and annotating secondary metabolites.
Article
Chemistry, Organic
Bum Soo Lee, Su Myung Jung, Rhim Ryoo, Sang Un Choi, Seongpil An, Ki Hyun Kim
Summary: In this study, a new dipeptide compound and three known components were isolated from the poisonous mushroom P. cornu-damae. The dipeptide compound exhibited potential anticancer activity, which is the first of its kind found in P. cornu-damae. These findings provide experimental evidence for the potential of the poisonous mushroom as a source of anticancer agents.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)