Article
Engineering, Biomedical
Omar Alheib, Lucilia P. da Silva, Alain da Silva Morais, Katia A. Mesquita, Rogerio P. Pirraco, Rui L. Reis, Vitor M. Conrelo
Summary: We developed an injectable gellan gum-based hydrogel that delivers C2C12 into a localized myopathic model. The gellan gum was biofunctionalized with a laminin-derived peptide to mimic the natural muscular extracellular matrix. Furthermore, the hydrogel was physically adjusted to replicate the mechanical properties of native tissue. This formula was utilized for the first time in the field of skeletal muscle tissue regeneration. The injectability of the hydrogel allowed for non-invasive administration, creating a reliable microenvironment that supported C2C12 with minimal inflammation. The treatment of skeletal muscle defects with the cell-laden hydrogel significantly enhanced regeneration of localized muscular trauma.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Applied
Shanyi Wu, Rongjun Xiao, Yong Wu, Laijun Xu
Summary: This review discusses the emerging advances and applications of Gellan gum (GG)-based hydrogels in tissue engineering, highlighting their potential in bone, cartilage, nerve, and retina tissue engineering. The review also explores the use of GG-based hydrogels as bioink components for 3D bioprinting.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
K. M. Kanyuck, T. B. Mills, I. T. Norton, A. B. Norton-Welch
Summary: This study investigated the swelling mechanism of high acyl gellan gum, its impact on hydrogel mechanical properties and drug release. Low ionic strength solutions caused the highest degree of swelling and slowed down glucose release, highlighting the importance of considering these changes in formulation design for functional applications.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Yong Qi, Shuyun Zhang, Yanni He, Shuanji Ou, Yang Yang, Yudun Qu, Jiaxuan Li, Wanmin Lian, Guitao Li, Junzhang Tian, Changpeng Xu
Summary: Due to its high biosafety, gellan gum (GG) hydrogel is commonly used in food and pharmaceuticals. In this study, mesoporous silica nanospheres (MMSN) and aldehyde-based methacrylated hyaluronic acid (AHAMA) were combined with sulfhydrated GG (TGG) to create a multi-network hydrogel. The resulting hydrogel exhibited good cell adhesion, high strength and elasticity, and great printability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Seongwon Lee, Joohee Choi, Jina Youn, Younghun Lee, Wooyoup Kim, Seungho Choe, Jeongeun Song, Rui L. Reis, Gilson Khang
Summary: This study investigated a ternary hydrogel composed of gellan gum, silk fibroin, and chondroitin sulfate as a biomaterial for cartilage tissue engineering. The hydrogel showed enhanced mechanical properties with higher gellan gum content, but exhibited harsh conditions for cell encapsulation at certain concentrations. The ternary hydrogel exhibited positive cell growth and expression of cartilage-specific ECM and genes, indicating its potential for promoting articular cartilage defect repair in cartilage tissue engineering.
Article
Cell & Tissue Engineering
Wooyoup Kim, Joo Hee Choi, Pilyun Kim, Jina Youn, Jeong Eun Song, Antonella Motta, Claudio Migliaresi, Gilson Khang
Summary: Recent research has focused on using biomaterials for cartilage regeneration, with gellan gum hydrogel (GG) identified as a promising material due to its water uptake ability and environmental similarity to native cartilage. However, challenges remain in modulating mechanical and cell adhesion properties. A study blending GG with silk fiber (SF) showed significant improvement in mechanical properties, making SF/GG a potential biomaterial for cartilage tissue engineering.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Engineering, Biomedical
Xiaoxue Wang, Jingliang Wu, Min Wang, Chunbo Lu, Wenfang Li, Qiao Lu, Yanying Li, Bo Lian, Bo Zhang
Summary: Recent research has focused on the development of drug-loaded hydrogels for wound healing. A carboxymethyl chitosan-gelatin hydrogel was chemically cross-linked using genipin as the cross-linking agent to deliver both substance P and DMOG. The synthesized hydrogels showed good biocompatibility and physicochemical properties, with the SP and DMOG combination promoting cell proliferation and facilitating wound healing in vivo. These findings suggest the potential application of this co-loaded drug hydrogel as a wound dressing material.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Polymer Science
Jorge Loureiro, Sonia P. Miguel, Victor Galvan-Chacon, David Patrocinio, Jose Blas Pagador, Francisco M. Sanchez-Margallo, Maximiano P. Ribeiro, Paula Coutinho
Summary: Myocardial infarction is a common cardiovascular disease and the leading cause of death worldwide. A 3D printed hydrogel, made from natural polymers, has been developed for cardiac tissue regeneration. The hydrogel, consisting of Gellan gum and Konjac glucomannan, shows excellent printability and possesses proper mechanical properties. It promotes endothelial cells' proliferation and migration, enabling the repair of injured cardiac tissue. This study provides the potential for a simple, low-cost, and efficient cardiac tissue regeneration method using a 3D printed hydrogel for myocardial infarction treatment in the future.
Article
Polymer Science
Yang Feng, Shao-Cong Dai, Khoon Lim, Yogambha Ramaswamy, Ahmad Jabbarzadeh
Summary: Polymeric PVA-based composite hydrogels were fabricated with gellan gum (GG) and their rheological and tribological properties were investigated. Increasing the concentration of GG enhanced the storage modulus of the hydrogels, making them suitable for cartilage regeneration applications. Furthermore, the addition of GG reduced the friction coefficient, increasing the chance of survival under mechanical shear stresses.
Article
Chemistry, Applied
Ziming Yang, Chen Guan, Chuang Zhou, Qingyan Pan, Zuyu He, Chao Wang, Yunhao Liu, Shuhui Song, Lijuan Yu, Yunhui Qu, Puwang Li
Summary: In this study, an active edible fresh-keeping material was developed using synthesized amphiphilic chitosan and carboxymethyl modified gellan gum. The optimal weight ratio of CMCS-g-CA/CMGG was determined as 5:2. The addition of mustard essential oil improved the water vapor and oxygen permeability of the composite films, and also enhanced the antibacterial properties against E. coli, S. aureus, and Bacillus anthracis. The CMCS-g-CA/CMGG + 2.0 mu L/mL MEO composite film effectively preserved mango fruits during 20 days of storage, making it a promising eco-friendly packaging material for fruit preservation.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Biomedical
Omar Alheib, Lucilia P. da Silva, David Caballero, Ricardo A. Pires, Subhas C. Kundu, Vitor M. Correlo, Rui L. Reis
Summary: Current therapies for skeletal muscle disorders/injuries are limited, necessitating the development of new treatments. Skeletal muscle tissue engineered platforms could provide valuable insights into the pathophysiology of skeletal muscle disorders/injuries and the efficacy of new therapies.
Article
Chemistry, Applied
Zhiping Fan, Ping Cheng, Yan Gao, Dawei Wang, Guangwei Jia, Pan Zhang, Sangeeta Prakash, Zhengping Wang, Jun Han
Summary: This research highlights the engineering of novel composite hydrogels from natural polysaccharides Salecan and Gellan using physical methods, without any structural modification or chemical crosslinking. The rheological properties of the hydrogels were investigated and key parameters were explored and compared using different models like Power Law, Herschel-Bulkley, and Arrhenius. This study reports the development of a novel composite hydrogel with ideal elasticity, injectability, good thermostability, and self-recovery, paving the way for its application in various fields.
FOOD HYDROCOLLOIDS
(2022)
Article
Biochemistry & Molecular Biology
Maryam Sahraro, Mehdi Barikani, Hamed Daemi, Payam Baei
Summary: In this study, multifunctional polyurethane nanoparticles (MPUNs) were embedded into methacrylated gellan gum to prepare stimuli-responsive hydrogels with improved mechanical properties. The optimized formulation showed excellent compression strength and ultimate strain, as well as the ability to restore its original shape after repeated compression tests. The hydrogels exhibited a dual physical and chemical crosslinking mechanism and were found to be non-toxic to human dermal fibroblast cells, making them suitable for soft tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Linyuan Liu, Duoduo Zhang, Xiaoxiao Song, Mi Guo, Ziwei Wang, Fang Geng, Xingtao Zhou, Shaoping Nie
Summary: In this study, the encapsulation of anthocyanins with Gelatin/Gellan gum was investigated to protect anthocyanins from degradation in the stomach and enhance their release in the small intestine. The results showed that Gelatin/Gellan gum = 8/2 had the most compact and stable structure, and the compound hydrogels did not inhibit the proliferation of normal rat intestinal epithelial cells (IEC-6), indicating the effectiveness and safety of this method.
FOOD HYDROCOLLOIDS
(2022)
Article
Biochemistry & Molecular Biology
Peng Wang, Zhi-gang Luo, Zhi-gang Xiao, Ahmed S. M. Saleh
Summary: Oxidized gellan gum (OGG) hydrogel beads were used as delivery systems for resveratrol. The degree of oxidation (DO) and CaCl2 concentration had significant effects on the formation and functional properties of hydrogel beads. The resveratrol encapsulation efficiency and loading capacity of OGG hydrogel beads increased with higher DO. The hydrogel beads showed a uniform spherical shape and were formed through hydrogen bonds and ionic interaction. Encapsulation of resveratrol changed its physical state from crystalline to amorphous form. Hydrogel beads fabricated with DO80 OGG and 1.0 M CaCl2 provided high protection and maintained the antioxidant capacity of resveratrol. DO and CaCl2 concentrations controlled the in-vitro release behaviors of hydrogel beads and achieved targeted release of resveratrol in the small intestine.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)