Article
Microbiology
In Young Choi, Jee-Hwan Oh, Zhiying Wang, Jan-Peter van Pijkeren
Summary: Biotherapeutic strategies, such as using engineered microbes to deliver therapeutic molecules, have great potential. However, the lack of precise tools to detect these microbes and their products hinders our ability to monitor and adjust therapeutic delivery. In this study, a bioluminescent peptide tagging system was adapted for lactic acid bacteria, which are commonly used as delivery vehicles for therapeutics. This system allowed sensitive detection of recombinant therapeutic proteins and could be used in continuous-culture bioreactor systems. Furthermore, it enabled rapid detection of administered microbes in intestinal and fecal samples.
Review
Chemistry, Applied
Jiayi Wu, Yuheng Zhang, Ling Ye, Chenglin Wang
Summary: Probiotic lactic acid bacteria EPS have potential anticancer effects by modulating tumor development through various mechanisms, and the future challenge lies in elucidating the precise structure-function relationship.
CARBOHYDRATE POLYMERS
(2021)
Article
Agriculture, Dairy & Animal Science
Adam Lee, Stephen C. C. Mansbridge, Lu Liang, Ian F. F. Connerton, Kenneth H. H. Mellits
Summary: New-born piglets that fail to thrive during suckling may suffer from lack of nutrition due to excessive piglet production values. The use of commercial milk replacers can provide nutrition but lacks milk sugars that contribute to immune system and gut microbiota development. This study investigated the effects of supplementing milk replacer with milk sugars on gut health, microbiome, and immune-protective goblet cells in piglets. The results showed that milk replacers supplemented with milk sugars improved gut health, increased beneficial gut bacteria, and enhanced the numbers of protective immune goblet cells. This suggests that milk sugars in milk replacers can play a beneficial role in the husbandry of poorly performing piglets moved to controlled environments.
Review
Food Science & Technology
Ruchi Sharma, Samira Mokhtari, Seid Mahdi Jafari, Somesh Sharma
Summary: Consumers worldwide are increasingly focusing on the link between nutrition and health, with probiotics, prebiotics, and synbiotics playing a key role in functional foods. Barley is highlighted as a potential substrate for probiotic products due to its nutrient content and ability to support Lactobacilli in the gastrointestinal tract.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Review
Food Science & Technology
Jagrani Minj, Priyanka Chandra, Catherine Paul, Rakesh Kumar Sharma
Summary: Lactic acid bacteria play crucial roles as starter cultures and probiotics in the fermentation and processing of dairy and food products, with various bio-functional properties that benefit human health. Their applications not only enhance the functionality of food products, but also have potential effects such as anti-inflammatory, antioxidant, and antimicrobial activities.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Jianlong Li, Kaidi Hu, Lu Hu, Xiaoyan Hou, Qin Li, Aiping Liu, Shujuan Chen, Xiaolin Ao, Xinjie Hu, Li He, Huaqiao Tang, Daomei Huang, Yong Yang, Likou Zou, Shuliang Liu
Summary: 3-PBA, a major degradation intermediate of pyrethroids, poses a severe threat to the ecosystem and human health due to its widespread existence in the environment. This study evaluated the adsorption capacity of L. plantarum RS20 towards 3-PBA and found that strain RS20 could be employed as a biological detoxification agent for humans and animals by eliminating 3-PBA from foods, feeds, and the digestive tract.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Dharmendra Kumar, Milan Kumar Lal, Som Dutt, Pinky Raigond, Sushil Sudhakar Changan, Rahul Kumar Tiwari, Kumar Nishant Chourasia, Vikas Mangal, Brajesh Singh
Summary: The current trend of health-conscious consumers and healthy food habits has led researchers to explore the development of food products with synbiotic benefits. This review article explores the alternative sources of probiotics and highlights various methods for making non-dairy synbiotics based on dietary fibers.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Noeli Sirini, Paula Loyeau, Maria Ruiz, Maria Stegmayer, Lorena Soto, Maria Werning, Laureano Frizzo, Vanesa Ordonez, Juana Fernandez-Lopez, Marcelo Rosmini
Summary: The viability of microencapsulated and unencapsulated Lactiplantibacillus plantarum BFL and their effects on the microbiological and physicochemical parameters of fermented sausages were studied in this research. The results showed that the inoculated sausages had lower pH values and potential pathogenic microorganism counts. Encapsulated L. plantarum BFL had better viability during storage compared to its unencapsulated state. In addition, L. plantarum BFL exhibited an antioxidant effect at 20 degrees C towards the end of storage. Overall, L. plantarum BFL could be used as a biocontroller in the meat industry due to its high viability rates.
FERMENTATION-BASEL
(2022)
Article
Immunology
Diogo Contente, Patricia Diaz-Rosales, Javier Feito, Lara Diaz-Formoso, Felix Docando, Rocio Simon, Juan Borrero, Pablo E. Hernandez, Patricia Poeta, Estefania Munoz-Atienza, Luis M. Cintas, Carolina Tafalla
Summary: Lactic Acid Bacteria (LAB) are frequently used as probiotics in aquaculture and have been shown to positively affect the growth, survival rate, and immunological status of fish. LAB produce antimicrobial peptides called bacteriocins, which have been well-studied for their antimicrobial properties. However, the direct immunomodulatory effects of bacteriocins in fish have not been extensively explored. In this study, the immunomodulatory effects of different bacteriocin-producing LAB strains were compared, and it was found that these strains had varying effects on immune functions, with nisin Z being the most potent immunomodulator.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Meyli Claudia Escobar-Ramirez, Gabriela Mariana Rodriguez-Serrano, Eduardo Zuniga-Leon, Mario Adolfo Garcia-Montes, Emmanuel Perez-Escalante, Luis Guillermo Gonzalez-Olivares
Summary: Lactic acid bacteria (LAB) exhibit resistance to sodium selenite concentrations above 100 mg/L. This study confirmed the presence of selenocysteine in Enterococcus faecium ABMC-05. The microorganism was cultured in a medium with a minimum inhibitory concentration of 184 mg/L sodium selenite, and the absorption of selenium and its conversion into selenocysteine were determined using ICP-OES and RP-HPLC, respectively. PCR analysis revealed the presence of the cysK gene and the absence of the selD and selA genes. The results demonstrate that this microorganism produces selenocysteine through a pathway independent of SelA and SelD, unlike other LAB.
FERMENTATION-BASEL
(2023)
Review
Biochemistry & Molecular Biology
Seon-Joo Park, Anshul Sharma, Hae-Jeung Lee
Summary: Overweight and obesity are global public health concerns that are increasing rapidly. Probiotics have been shown to have benefits against obesity, and postbiotics, which are bacteria-free extracts and nonviable microorganisms, are being favored over regular probiotics. This review gathers comprehensive data on the ability of postbiotics to combat obesity and discusses their anti-obesity effects in cellular, animal, and clinical studies, suggesting that postbiotics could be a promising dietary intervention for obesity prevention and management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Soyoung Park, Kandasamy Saravanakumar, Anbazhagan Sathiyaseelan, SeonJu Park, Xiaowen Hu, Myeong-Hyeon Wang
Summary: The present study isolated and identified five lactic acid bacteria from Korean kimchi, with Weissella cibaria producing a high level of exopolysaccharide (EPS) compared to other isolates. The EPS from W. cibaria was found to be composed of glucose and galactose, linked through specific monosaccharides. It exhibited strong antioxidant activity and biocompatibility, making it a promising candidate for biomedical applications.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ronaldo Rwubuzizi, Hamin Kim, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: The role of lactic acid bacteria (LAB) as probiotics in promoting human or veterinary health has attracted increasing interest. Screening approaches have been used to identify LAB strains that meet minimal requirements for safety and functionality, including natural antioxidant properties. In this study, several in vitro antioxidant assays were conducted to evaluate the antioxidant properties of different LAB strains, and some showed promising results that could potentially be applied as natural antioxidants in fermented food products.
Article
Agronomy
Mihaela Dumitru, Nicoleta Aurelia Lefter, Mihaela Habeanu, Georgeta Ciurescu, Dan C. Vodnar, Simon Elemer, Ionut Sorescu, Sergiu Emil Georgescu, Andreea Dudu
Summary: Fourteen lactic acid bacteria (LAB) strains were isolated from the intestinal tract and feces of piglets, and only 12 strains were confirmed as Limosilactobacillus fermentum, Lactobacillus acidophilus, and Lactiplantibacillus plantarum. Two strains, L. acidophilus IBNA 76 and L. plantarum IBNA 84, showed high survival rates under low pH and bile salts conditions. They also exhibited resistance to multiple antibiotics. After encapsulation using the spray-drying technique, the probiotic viability decreased but still remained promising. These findings suggest that L. acidophilus IBNA 76 and L. plantarum IBNA 84 could be considered as probiotic candidates for piglet nutrition.
Article
Biotechnology & Applied Microbiology
Buket Kunduhoglu, Seda Hacioglu
Summary: This study explored the gluten hydrolysis ability and probiotic potential of Lact. brevis KT16-2, which showed promising results in terms of surviving in adverse conditions, producing antimicrobial peptides, and exhibiting high antioxidant activity.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Gastroenterology & Hepatology
Cristina Sole, Susie Guilly, Kevin Silva, Marta Llopis, Emmanuelle Le-Chatelier, Patricia Huelin, Marta Carol, Rebeca Moreira, Nuria Fabrellas, Gloria De Prada, Laura Napoleone, Isabel Graupera, Elisa Pose, Adria Juanola, Natalia Borruel, Magali Berland, David Toapanta, Francesc Casellas, Francisco Guarner, Joel Dore, Elsa Sola, Stanislav Dusko Ehrlich, Pere Gines
Summary: Patients with cirrhosis exhibit significant alterations in gut microbiome, particularly pronounced in those with ACLF, correlated with disease stages and complications, and predictive of survival. These findings suggest a potential role of gut microbiome in disease progression and prognosis, warranting further validation in future studies.
Review
Gastroenterology & Hepatology
Julia Alvarez, Jose Manuel Fernandez Real, Francisco Guarner, Miguel Gueimonde, Juan Miguel Rodriguez, Miguel Saenz de Pipaon, Yolanda Sanz
Summary: The human body is inhabited by numerous microorganisms, especially in the intestine, which play a crucial role in various physiological processes. Some chronic diseases in developed societies are associated with dysbiosis, which may be linked to changes in microbiome transmission, use of antibiotics, and dietary habits. Therefore, maintaining diversity in the microbiota is essential for health promotion and disease prevention.
GASTROENTEROLOGIA Y HEPATOLOGIA
(2021)
Correction
Gastroenterology & Hepatology
Manuel Barreiro-de Acosta, Ignacio Marin-Jimenez, Iago Rodriguez-Lago, Francisco Guarner, Eloy Espin, Isabel Ferrer Bradley, Ana Gutierrez, Belen Beltran, Maria Chaparro, Javier P. Gisbert, Pilar Nos
GASTROENTEROLOGIA Y HEPATOLOGIA
(2021)
Article
Nutrition & Dietetics
Claudia Barber, Marianela Mego, Carlos Sabater, Fernando Vallejo, Rogger Alvaro Bendezu, Marcela Masihy, Francisco Guarner, Juan Carlos Espin, Abelardo Margolles, Fernando Azpiroz
Summary: The study compared the effects of a fibre-enriched Mediterranean diet and a Western-type diet on gut microbiota, digestive function, and sensations in healthy men. Despite minimal differences in microbiota composition between the diets, there were significant variations in microbial metabolism and metabolic pathways. Individuals with more stable microbiotas showed less noticeable effects of dietary changes.
Article
Biochemistry & Molecular Biology
Sara Notararigo, Encarnacion Varela, Anna Otal, Maria Antolin, Francisco Guarner, Paloma Lopez
Summary: Bacterial beta-glucans are polysaccharides that can protect bacteria or participate in biofilm formation and bacterial cell adhesion. This study found that the O-2-substituted (1-3)-beta-D-glucan synthesized by Pediococcus parvulus 2.6 has a similar structure to laminarin and has the ability to modulate the immune system and adhere to human enterocytes. The study also revealed that this glucan is non-toxic to cells and can regulate cytokine production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Boris Le Neve, Adrian De la Torre, Julien Tap, Adoracion Nieto Ruiz, Muriel Derrien, Aurelie Cotillard, Jean-Michel Faurie, Elizabeth Barba, Marianela Mego, Quentin Dornic, John Butler, Xavi Merino, Beatriz Lobo, Ferran Pinsach Batet, Marta Pozuelo, Javier Santos, Francisco Guarner, Chaysavanh Manichanh, Fernando Azpiroz
Summary: This study aimed to assess the effects of a fermented milk product containing probiotics on the tolerance of a healthy diet in patients with disorders of gut-brain interactions (DGBI). The results showed that the consumption of this product could reduce the sensation of flatulence and the daily number of anal gas evacuations, potentially mediated by the metabolic activity of the gut microbiota.
Review
Gastroenterology & Hepatology
Zarina Mamieva, Elena Poluektova, Valery Svistushkin, Vasily Sobolev, Oleg Shifrin, Francisco Guarner, Vladimir Ivashkin
Summary: Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder with incompletely understood etiology. Dysbiosis of the gut microbiota and antibiotic use are closely associated with the development of IBS.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Gastroenterology & Hepatology
Lissette Batista, Virginia Robles, Chaysavanh Manichanh, Laura Ruiz, Danila Guagnozzi, Ferran Pinsach, Francisco Guarner, Fernando Fernandez-Banares
Summary: Colonic lavage with PEG did not have a significant impact on the clinical remission rate, microbial diversity, and dysbiosis index in patients with active microscopic colitis. The microbial changes in microscopic colitis may be associated with stool form.
BMC GASTROENTEROLOGY
(2022)
Article
Pharmacology & Pharmacy
Nuno Correia, Francisco Guarner, Georgina Logusso
Summary: This study aimed to investigate the changes in quality of life and perception of effectiveness in Spanish consumers after purchasing and using Macrogol 4000 to treat constipation symptoms. The results showed a significant reduction in physical and psychosocial discomfort after 14 days of use, along with improved responses on effectiveness perception questionnaires.
Review
Medicine, Research & Experimental
Carlos Patricio Acosta-Rodriguez-Bueno, Ana Teresa Abreu Y. Abreu, Francisco Guarner, Mary Jean Guno, Ender Pehlivanoglu, Marcos Perez
Summary: This article reviews the characteristics and mechanisms of action of B. clausii as a probiotic, as well as clinical studies evaluating its use in the management of gastrointestinal disorders. More research evidence is needed to guide the clinical application of probiotics for gastrointestinal disorders and other conditions.
ADVANCES IN THERAPY
(2022)
Article
Nutrition & Dietetics
Claudia Barber, Carlos Sabater, Maria Angeles Avila-Galvez, Fernando Vallejo, Rogger Alvaro Bendezu, Laetitia Guerin-Deremaux, Francisco Guarner, Juan Carlos Espin, Abelardo Margolles, Fernando Azpiroz
Summary: Resistant dextrin affects gut microbiota metabolism and composition, reducing gas production and providing health benefits in blood glucose management and satiety.
Article
Gastroenterology & Hepatology
Claudia Herrera-deGuise, Encarna Varela, Guillaume Sarrabayrouse, Marta Pozuelo del Rio, Virginia Robles Alonso, Natalia Borruel Sainz, Francesc Casellas, Luis Fernando Mayorga, Chaysavanh Manichanh, Fernando Azpiroz Vidaur, Francisco Guarner
Summary: The gut microbial ecosystem in ulcerative colitis (UC) patients with long-term remission shows similarities to healthy individuals. Patients with short-term remission or disease flare exhibit reduced diversity and richness of gut microbiota, as well as an increase in potentially harmful bacteria. Patients in long-term remission have a composition of fecal bacteria similar to healthy controls, and the presence of A. muciniphila in fecal samples predicts flare-free outcomes.
INFLAMMATORY BOWEL DISEASES
(2023)
Article
Nutrition & Dietetics
Claudia Barber, Carlos Sabater, Francisco Guarner, Abelardo Margolles, Fernando Azpiroz
Summary: This study examined the fermentation and adaptation response of the gut microbiota to guar gum consumption. The results showed that guar gum is metabolized by intestinal microbiota and induces selective adaptation of microbial taxonomy and function upon continuous consumption. These findings suggest the potential interest of guar gum for novel prebiotic ingredient formulation.
FRONTIERS IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Gerard Serrano-Gomez, Luis Mayorga, Inigo Oyarzun, Joaquim Roca, Natalia Borruel, Francesc Casellas, Encarna Varela, Marta Pozuelo, Kathleen Machiels, Francisco Guarner, Severine Vermeire, Chaysavanh Manichanh
Summary: Crohn's disease (CD) is more dysbiotic than ulcerative colitis (UC), while CD relapse is associated with alterations at the species and metabolic pathway levels, providing biomarkers to discriminate IBD subtypes and predict disease activity. Our study emphasizes the importance of considering relapse-related microbial dysbiosis in IBD patients for better management and treatment strategies.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Meeting Abstract
Gastroenterology & Hepatology
Claudia Herrera-deGuise, Guillaume Sarrabayrouse, Encarna Varela, Virginia Robles, Natalia Borruel, Luis F. Mayorga, Francesc Casellas, Francisco Guarner
