Article
Biochemistry & Molecular Biology
Kai Zhao, Agnes Schaefer, Zhuo Zhang, Katharina Elsaesser, Carsten Culmsee, Li Zhong, Axel Pagenstecher, Christopher Nimsky, Joerg W. Bartsch
Summary: Carbonic anhydrase CA2 is highly expressed in recurrent glioblastoma and temozolomide-resistant glioblastoma stem-like cells (GSCs), promoting cell invasion. The CA2 inhibitor brinzolamide is more effective than the pan-CA inhibitor acetazolamide in sensitizing GSCs to TMZ-induced cell death. The combined treatment of TMZ and brinzolamide induces autophagy in GBM cell lines and GSCs, suggesting a potential treatment strategy for tackling GBM chemoresistance and recurrence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Baptiste Gouyou, Katja Gruen, Anne Kerschenmeyer, Alessandra Villa, Mattia Matasci, Andrea Schrepper, Alexander Pfeil, Laura Baez, Christian Jung, P. Christian Schulze, Dario Neri, Marcus Franz
Summary: This study evaluated the therapeutic activity of F8IL9F8 in attenuating PH progression using a monocrotaline mouse model. Results showed that F8IL9F8 and ERA treatments significantly reduced systolic right ventricular pressure and improved echocardiographic parameters compared to the control group, while the KSFIL9KSF group did not show the same effects. F8IL9F8 also significantly reduced lung tissue damage and decreased leukocyte and macrophage accumulation in the lungs and right ventricles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Edikan A. Ogunnaike, Alain Valdivia, Mostafa Yazdimamaghani, Ernesto Leon, Seema Nandi, Hannah Hudson, Hongwei Du, Simon Khagi, Zhen Gu, Barbara Savoldo, Frances S. Ligler, Shawn Hingtgen, Gianpietro Dotti
Summary: The delivery of CAR-T cells within the surgical cavity using a fibrin-based gel demonstrates superior antitumor activity compared to direct inoculation into the tumor resection cavity in a model of subtotal glioblastoma resection.
Article
Cell Biology
Malgorzata Grabowska, Konrad Kuczynski, Monika Piwecka, Alicja Rabiasz, Pawel Glodowicz, Dariusz Wawrzyniak, Malgorzata Lekka, Katarzyna Rolle
Summary: Glioblastoma is a common malignant brain tumor characterized by the ability of its cells to infiltrate surrounding brain tissue. This study demonstrates that the microRNA miR-218 is involved in the regulation of extracellular matrix (ECM) components, and its overexpression leads to changes in the biomechanical properties of GBM cells. These changes include impairing migration potential and enhancing adhesive properties of the cells. Additionally, miR-218 expression level affects cell stiffness and cytoskeletal reorganization.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Chemistry, Analytical
Samantha M. M. McCabe, Gregory Q. Q. Wallace, Sian Sloan-Dennison, William J. J. Tipping, Neil C. C. Shand, Duncan Graham, Marie Boyd, Karen Faulds
Summary: This study investigates the uptake and distribution of silica coated gold nanoparticles (AuNP-SHINs) in three-dimensional multicellular tumour spheroids derived from glioblastoma cells. The results show that the localization of nanoparticles within the spheroids is influenced by factors such as size, quiescence, and hypoxia.
Article
Biotechnology & Applied Microbiology
Jie Yu, Lin Han, Feng Yang, Mingliang Zhao, Hong Zhou, Linwang Hu
Summary: The high expression of SOCS5 is associated with poor prognosis and resistance to TMZ in GBM. TMZ treatment increases the expression of SOCS5 and induces autophagy, which is mediated by SOCS5. Mechanistically, SOCS5 enhances the transcription of Bcl-2. Knockdown of SOCS5 reduces TMZ chemoresistance in GBM cells by inhibiting Bcl-2-mediated autophagy.
Article
Multidisciplinary Sciences
Eduardo Ruiz-Lopez, Ivana Jovcevska, Ruth Gonzalez-Gomez, Hector Tejero, Fatima Al-Shahrour, Serge Muyldermans, Alberto J. Schuhmacher
Summary: The lack of non-invasive biomarkers and the blood-brain barrier restrictions hinder the translation of cancer omics findings for glioblastomas. However, nanobodies emerge as a promising tool for immunotargeted applications. In this study, researchers identified ABCC3 as a suitable target for immunotargeted applications using bioinformatic analysis, and successfully isolated nanobodies that selectively recognize ABCC3 in glioblastoma.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Andrew J. Brenner, John Floyd, Lisa Fichtel, Joel Michalek, Kunal P. Kanakia, Shiliang Huang, David Reardon, Patrick Y. Wen, Eudocia Quant Lee
Summary: The study investigated the safety and efficacy of Evo in combination with bevacizumab for treating bevacizumab refractory glioblastoma patients. Results showed a median overall survival of approximately 4.6 months with a median time to disease progression of 53 days from best response. The progression free survival at 4 months (PFS-4) on Evo-Bev treatment demonstrated a statistically significant improvement.
SCIENTIFIC REPORTS
(2021)
Article
Polymer Science
Kang Xu, Long Zhang, Yuqi Gu, Huasha Yang, Baixiang Du, Hongmei Liu, Yuling Li
Summary: The synthesis of PEtOz-TMZ conjugate has shown enhanced efficacy and stability of TMZ in vivo, indicating a promising route of administration for TMZ in clinical practice.
EUROPEAN POLYMER JOURNAL
(2021)
Review
Oncology
Olga Renner, Markus Burkard, Holger Michels, Claudia Vollbracht, Tobias Sinnberg, Sascha Venturelli
Summary: Standard therapy for glioblastoma typically results in limited survival and quality of life improvements, but pharmacological ascorbate shows potential efficacy. The biological complexity and resistance of glioblastoma calls for further research to confirm ascorbate's potential as a therapeutic agent.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Alexandra M. Amen, Christof Fellmann, Katarzyna M. Soczek, Shawn M. Ren, Rachel J. Lew, Gavin J. Knott, Jesslyn E. Park, Andrew M. McKinney, Andrew Mancini, Jennifer A. Doudna, Joseph F. Costello
Summary: Mutations in the TERT promoter of glioblastomas create binding sites for GABP transcription factor complexes, leading to reactivation of TERT and maintenance of telomeres. GBM cells with TERT promoter mutations display a dependence on GABPB1L for proliferation, which can be rescued by upregulation of the protein paralogue GABPB2. Inhibition of GABPB1L in combination with chemotherapy results in reduced growth of intracranial GBM tumors by impairing DNA damage response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Tiziano Ongaro, Salvatore R. Guarino, Luigi Scietti, Martina Palamini, Sarah Wulhfard, Dario Neri, Alessandra Villa, Federico Forneris
Summary: The use of immunomodulatory agents for cancer treatment is gaining biopharmaceutical interest. Structural techniques can provide insights into the 3D arrangement of antibody-cytokine fusion proteins, which can affect their biological activity and pharmacokinetic properties. Low-resolution molecular structure characterizations are useful for quality control of immunocytokines and can guide the design and optimization of these chimeric protein reagents.
JOURNAL OF STRUCTURAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Christian T. Stackhouse, Joshua C. Anderson, Zongliang Yue, Thanh Nguyen, Nicholas J. Eustace, Catherine P. Langford, Jelai Wang, James R. Rowland, Chuan Xing, Fady M. Mikhail, Xiangqin Cui, Hasan Alrefai, Ryan E. Bash, Kevin J. Lee, Eddy S. Yang, Anita B. Hjelmeland, C. Ryan Miller, Jake Y. Chen, Yancey Gillespie, Christopher D. Willey
Summary: The key molecular regulators of acquired radiation resistance in recurrent glioblastoma (GBM) are largely unknown. In this study, patient-derived xenograft (PDX) models were generated to mimic the longitudinal evolution of patient recurrent tumors following radiation therapy. Whole-exome sequencing and bioinformatics analysis identified genomic alterations and kinomic alterations associated with acquired radiation resistance. The study also found multiple molecular routes to acquired radiation resistance and identified targetable kinases for potential therapeutic interventions. This research provides important insights into overcoming treatment resistance in GBM patients.
Article
Chemistry, Medicinal
Meng-Meng Zhang, Yan Jia, Peng Li, Yan Qiao, Ke-Li Han
Summary: The study found that lysosomal sulfatase expression is closely related to the prognosis of glioblastoma. A new probe, MNG, was developed to detect lysosomal sulfatase, and the first reported lysosomal sulfatase inhibitor, sulbactam, was found to inhibit tumor growth in GBM.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Katharina Otte, Kai Zhao, Madita Braun, Andreas Neubauer, Hartmann Raifer, Frederik Helmprobst, Felipe Ovalle Barrera, Christopher Nimsky, Joerg W. Bartsch, Tillmann Rusch
Summary: This study found that Eltanexor, a second-generation XPO1 inhibitor, has effective monotherapy effects on GBM cells and can be combined with current adjuvant therapies to provide a more effective treatment for GBM. The mechanism of Eltanexor-induced apoptosis in GBM cells may be related to increased expression of TP53-dependent genes.
Correction
Medicine, General & Internal
Elisa Gesu, Pietro Bellone, Mattia Bonzi, Giulio Andrea Bertani, Barbara Brignolo Ottolini, Paola Bosco, Giorgio Conte, Matteo Ferrari, Elisa Maria Fiorelli, Hayato Kurihara, Monica Solbiati, Luigi Piero Solimeno, Giorgio Costantino
INTERNAL AND EMERGENCY MEDICINE
(2022)
Letter
Genetics & Heredity
Katy Phelan, Luigi Boccuto, Craig M. Powell, Tobias M. Boeckers, Conny van Ravenswaaij-Arts, R. Curtis Rogers, Carlo Sala, Chiara Verpelli, Audrey Thurm, William E. Bennett, Christopher J. Winrow, Sheldon R. Garrison, Roberto Toro, Thomas Bourgeron
Summary: Phelan-McDermid syndrome, initially known as 22q13 deletion syndrome, is now recognized to be associated with SHANK3 gene. To reduce confusion, a simple classification system has been proposed, dividing PMS into PMS-SHANK3 related and PMS-SHANK3 unrelated cases.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Clinical Neurology
Giorgio Fiore, Giulio A. Bertani, Leonardo Tariciotti, Stefano Borsa, Aldo Paolucci, Lucia Taramasso, Marco Locatelli, Mauro Pluderi
Summary: Vertebral hemangioma resection is a challenging procedure for spine surgeons due to the risk of massive bleeding. Preoperative transarterial embolization is recommended to reduce bleeding risk. In this article, the authors discuss an unusual clinical and radiologic presentation following an endovascular procedure and provide an explanation for the findings.
JOURNAL OF NEUROLOGICAL SURGERY PART A-CENTRAL EUROPEAN NEUROSURGERY
(2023)
Article
Genetics & Heredity
Stefania Beretta, Laura Gritti, Luisa Ponzoni, Paolo Scalmani, Massimo Mantegazza, Mariaelvina Sala, Chiara Verpelli, Carlo Sala
Summary: The study found that genetic deletion and pharmacological inhibition of eEF2K can improve the epileptic phenotype of Dravet syndrome mice, and also reverse their motor coordination defects, memory impairments, and stereotyped behavior. This study provides a new possibility for the treatment of Dravet syndrome.
Article
Biochemistry & Molecular Biology
Asad Jamal, Tian Yuan, Stefano Galvan, Antonella Castellano, Marco Riva, Riccardo Secoli, Andrea Falini, Lorenzo Bello, Ferdinando Rodriguez Y. Baena, Daniele Dini
Summary: This article focuses on the application of infusion-based targeted drug delivery in the treatment of brain diseases such as glioblastoma multiforme. The author emphasizes the biomechanical and biochemical aspects of drug delivery and discusses the challenges and advances in medical robotics in targeted drug delivery in the brain. Additionally, the critical overview of current research and its clinical implications is provided.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Clinical Neurology
Leonardo Tariciotti, Paolo Palmisciano, Martina Giordano, Giulia Remoli, Eleonora Lacorte, Giulio Bertani, Marco Locatelli, Francesco Dimeco, Valerio M. Caccavella, Francesco Prada
Summary: This review summarizes the applications of artificial intelligence and machine learning in neurosurgical surgeries, including preoperative planning and intraoperative assistance. The review found that ML models can improve surgical team performances by reducing human errors and providing patient-tailored surgical plans.
JOURNAL OF NEUROSURGICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Stefano Musardo, Sebastien Therin, Silvia Pelucchi, Laura D'Andrea, Ramona Stringhi, Ana Ribeiro, Annalisa Manca, Claudia Balducci, Jessica Pagano, Carlo Sala, Chiara Verpelli, Valeria Grieco, Valeria Edefonti, Gianluigi Forloni, Fabrizio Gardoni, Giovanni Meli, Daniele Di Marino, Monica Di Luca, Elena Marcello
Summary: The development of new therapeutic avenues targeting early stages of Alzheimer's disease is urgently needed. Research has demonstrated that interfering with the endocytosis of ADAM10 using PEP3 can restore its postsynaptic localization and activity, thereby improving cognitive defects. However, further studies are required to evaluate the long-term efficacy and safety of PEP3 administration.
Article
Neurosciences
Luca Fornia, Marco Rossi, Marco Rabuffetti, Andrea Bellacicca, Luca Vigano, Luciano Simone, Henrietta Howells, Guglielmo Puglisi, Antonella Leonetti, Vincenzo Callipo, Lorenzo Bello, Gabriella Cerri
Summary: The study found that different regions of the parietal cortex have varying effects on hand muscle control and manipulation, suggesting the presence of different functional sectors within the parietal cortex. The task-arrest pattern may be associated with parietal sectors more closely implicated in shaping motor output.
Article
Clinical Neurology
Giorgio Fiore, Leonardo Tariciotti, Stefano Borsa, Daniele Nicoli, Luigi Schisano, Giulio Andrea Bertani, Marco Locatelli, Mauro Pluderi
Summary: The study suggests that posterior percutaneous cement-augmented screws short fixation (PASF) could be a safe and effective treatment option for severe osteoporotic vertebral burst fractures when conservative treatments have failed.
WORLD NEUROSURGERY
(2022)
Article
Biochemistry & Molecular Biology
Debora Garrido, Stefania Beretta, Stefanie Grabrucker, Helen Friedericke Bauer, David Bayer, Carlo Sala, Chiara Verpelli, Francesco Roselli, Juergen Bockmann, Christian Proepper, Alberto Catanese, Tobias M. Boeckers
Summary: Members of the Shank protein family are linked to autism spectrum disorders (ASDs). Researchers created a double knockout mouse model for Shank2 and Shank3 genes, which exhibited severe symptoms of autism and other comorbidities. By deleting Shank2 and Shank3, researchers identified the involvement of the retrosplenial area in social memory impairment, a core symptom of ASD. Neuronal activation could rescue the social impairment caused by Shank2/3 depletion.
MOLECULAR PSYCHIATRY
(2022)
Article
Health Care Sciences & Services
Matteo Marcatili, Riccardo Borgonovo, Noemi Cimminiello, Ranieri Domenico Cornaggia, Giulia Casati, Cristian Pellicioli, Laura Maggioni, Federico Motta, Chiara Redaelli, Luisa Ledda, Federico Emanuele Pozzi, Michaela Krivosova, Jessica Pagano, Roberto Nava, Fabrizia Colmegna, Antonios Dakanalis, Alice Caldiroli, Enrico Capuzzi, Beatrice Benatti, Bernardo Dell'Osso, Francesca Bertola, Nicoletta Villa, Alberto Piperno, Silvia Ippolito, Ildebrando Appollonio, Carlo Sala, Luciano Conti, Massimo Clerici
Summary: The advent of intra-nasal esketamine (ESK) has the potential to revolutionize the treatment of treatment resistant depression (TRD). However, some patients experience unstable responses and high recurrence rates. These patients require a complex multidisciplinary approach and can benefit from personalized medicine tools.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Clinical Neurology
Silvia Lanfranconi, Elisa Scola, Jennifer M. T. A. Meessen, Roberto Pallini, Giulio A. Bertani, Rustam Al-Shahi Salman, Elisabetta Dejana, Roberto Latini, Treat CCM Investigators
Summary: Background observations and preclinical models suggest that prolonged treatment with propranolol may reduce the risk of intracerebral hemorrhage in patients with familial cerebral cavernous malformations. A phase 2 pilot trial was conducted to evaluate the safety and efficacy of propranolol in reducing the incidence of symptomatic intracerebral hemorrhage or focal neurological deficit in this patient population.
Article
Clinical Neurology
Leonardo Tariciotti, Giorgio Fiore, Giorgio Carrabba, Giulio A. Bertani, Luigi Schisano, Stefano Borsa, Emanuele Ferrante, Valerio M. Caccavella, Pierpaolo Mattogno, Martina Giordano, Giulia Remoli, Giovanna Mantovani, Marco Locatelli
Summary: We developed a supervised machine learning model to predict the risk of intraoperative cerebrospinal fluid leakage during endoscopic transsphenoidal surgery for pituitary adenomas. The best performing algorithm was a random forest classifier, which showed high performance metrics in predicting surgical outcomes.
JOURNAL OF NEUROSURGICAL SCIENCES
(2023)
Review
Medicine, General & Internal
Giorgio Fiore, Edoardo Porto, Mauro Pluderi, Antonella Maria Ampollini, Stefano Borsa, Federico Giuseppe Legnani, Davide Giampiccolo, Anna Miserocchi, Giulio Andrea Bertani, Francesco DiMeco, Marco Locatelli
Summary: In this study, the effects of various drugs for pain prevention in adults undergoing craniotomy were analyzed. The results indicate that NSAIDs and acetaminophen may moderately reduce post-craniotomy pain at 24 hours, while ropivacaine scalp block may have a larger impact on pain reduction at 6 hours. NSAIDs were also found to have a significant effect on pain reduction at 12 hours. However, there is no effective treatment identified for post-craniotomy pain prevention at 48 hours.
MEDICINA-LITHUANIA
(2023)
